{"title":"从发酵茶叶中分离出的潜在益生菌植物乳杆菌 CRM56-2 的基因组评估","authors":"Engkarat Kingkaew, Naoto Tanaka, Yuh Shiwa, Jaruwan Sitdhipol, Rattanatda Nuhwa, Somboon Tanasupawat","doi":"10.21315/tlsr2024.35.2.12","DOIUrl":null,"url":null,"abstract":"<p><p><i>Lactiplantibacillus plantarum</i> is a widely studied species known for its probiotic properties that can help alleviate serum cholesterol levels. Whole-genome sequencing provides genetic information on probiotic attributes, metabolic activities and safety assessment. This study investigates the probiotic properties of strain CRM56-2, isolated from Thai fermented tea leaves, using Whole-Genome Sequencing (WGS) to evaluate the safety, health-promoting genes and functional analysis. Strain CRM56-2 showed bile salt hydrolase (BSH) activity, assimilated cholesterol at a rate of 75.94%, tolerated acidic and bile environments and attached to Caco-2 cells. Based on ANIb (98.9%), ANIm (99.2%), and digital DNA-DNA hybridisation (98.3%), strain CRM56-2 was identified as <i>L. plantarum</i>. <i>In silico</i> analysis revealed that it was not pathogenic and contained no antibiotic-resistance genes or plasmids. <i>L. plantarum</i> CRM56-2 possessed genes linked to several probiotic properties and beneficial impacts. The genome of strain CRM56-2 suggested that <i>L. plantarum</i> CRM56-2 is non-hazardous, with potential probiotic characteristics and beneficial impacts, which could enhance its probiotic application. Consequently, <i>L. plantarum</i> CRM56-2 demonstrated excellent cholesterol-lowering activity and probiotic properties.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371405/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genomic Assessment of Potential Probiotic <i>Lactiplantibacillus plantarum</i> CRM56-2 Isolated from Fermented Tea Leaves.\",\"authors\":\"Engkarat Kingkaew, Naoto Tanaka, Yuh Shiwa, Jaruwan Sitdhipol, Rattanatda Nuhwa, Somboon Tanasupawat\",\"doi\":\"10.21315/tlsr2024.35.2.12\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Lactiplantibacillus plantarum</i> is a widely studied species known for its probiotic properties that can help alleviate serum cholesterol levels. Whole-genome sequencing provides genetic information on probiotic attributes, metabolic activities and safety assessment. This study investigates the probiotic properties of strain CRM56-2, isolated from Thai fermented tea leaves, using Whole-Genome Sequencing (WGS) to evaluate the safety, health-promoting genes and functional analysis. Strain CRM56-2 showed bile salt hydrolase (BSH) activity, assimilated cholesterol at a rate of 75.94%, tolerated acidic and bile environments and attached to Caco-2 cells. Based on ANIb (98.9%), ANIm (99.2%), and digital DNA-DNA hybridisation (98.3%), strain CRM56-2 was identified as <i>L. plantarum</i>. <i>In silico</i> analysis revealed that it was not pathogenic and contained no antibiotic-resistance genes or plasmids. <i>L. plantarum</i> CRM56-2 possessed genes linked to several probiotic properties and beneficial impacts. The genome of strain CRM56-2 suggested that <i>L. plantarum</i> CRM56-2 is non-hazardous, with potential probiotic characteristics and beneficial impacts, which could enhance its probiotic application. Consequently, <i>L. plantarum</i> CRM56-2 demonstrated excellent cholesterol-lowering activity and probiotic properties.</p>\",\"PeriodicalId\":23477,\"journal\":{\"name\":\"Tropical life sciences research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371405/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical life sciences research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21315/tlsr2024.35.2.12\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/31 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical life sciences research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21315/tlsr2024.35.2.12","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
植物乳杆菌（Lactiplantibacillus plantarum）是一种被广泛研究的益生菌，其益生菌特性有助于降低血清胆固醇水平。全基因组测序提供了有关益生菌特性、代谢活动和安全性评估的基因信息。本研究利用全基因组测序技术（WGS）对从泰国发酵茶叶中分离出来的菌株 CRM56-2 的益生特性进行了研究，以评估其安全性、健康促进基因和功能分析。菌株CRM56-2具有胆盐水解酶（BSH）活性，胆固醇同化率高达75.94%，能耐受酸性和胆汁环境，并能附着在Caco-2细胞上。根据 ANIb（98.9%）、ANIm（99.2%）和数字 DNA-DNA 杂交（98.3%），菌株 CRM56-2 被鉴定为植物乳杆菌。硅学分析表明，该菌株不具致病性，不含抗生素耐药基因或质粒。L. plantarum CRM56-2 拥有与多种益生菌特性和有益影响相关的基因。菌株 CRM56-2 的基因组表明，L. plantarum CRM56-2 不具危害性，具有潜在的益生特性和有益影响，可提高其在益生菌中的应用。因此，植物乳杆菌CRM56-2表现出卓越的降胆固醇活性和益生特性。
Genomic Assessment of Potential Probiotic Lactiplantibacillus plantarum CRM56-2 Isolated from Fermented Tea Leaves.
Lactiplantibacillus plantarum is a widely studied species known for its probiotic properties that can help alleviate serum cholesterol levels. Whole-genome sequencing provides genetic information on probiotic attributes, metabolic activities and safety assessment. This study investigates the probiotic properties of strain CRM56-2, isolated from Thai fermented tea leaves, using Whole-Genome Sequencing (WGS) to evaluate the safety, health-promoting genes and functional analysis. Strain CRM56-2 showed bile salt hydrolase (BSH) activity, assimilated cholesterol at a rate of 75.94%, tolerated acidic and bile environments and attached to Caco-2 cells. Based on ANIb (98.9%), ANIm (99.2%), and digital DNA-DNA hybridisation (98.3%), strain CRM56-2 was identified as L. plantarum. In silico analysis revealed that it was not pathogenic and contained no antibiotic-resistance genes or plasmids. L. plantarum CRM56-2 possessed genes linked to several probiotic properties and beneficial impacts. The genome of strain CRM56-2 suggested that L. plantarum CRM56-2 is non-hazardous, with potential probiotic characteristics and beneficial impacts, which could enhance its probiotic application. Consequently, L. plantarum CRM56-2 demonstrated excellent cholesterol-lowering activity and probiotic properties.
期刊介绍：
Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.
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