Tropical life sciences research最新文献

{"title":"Thermal Relationship in Tropical Anurans from Two Contrasting Habitats Along an Elevation Gradient in Colombia","authors":"Katalina Gutiérrez Hernández, Carlos Alberto Galindo, Jorge Luis, Turriago González, Manuel Hernando Bernal Bautista, Carlos Alberto, Jorge Galindo, Luis Turriago, Gonzalez Manuel, Hernando, Carlos Alberto Galindo Martínez, Turriago González","doi":"10.21315/tlsr2024.35.1.12","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.12","url":null,"abstract":"Anurans are ectothermic organisms highly susceptible to variations in the environmental temperature that changes with elevation and between habitats in tropical mountains. The aim of this study was to evaluate the variation of body temperature (BT) of nocturnal anurans from two contrasting habitats (open and forest habitats) along an elevation gradient in Colombia. We measured the environmental temperatures (substrate and air) and BT of 135 adult frogs of 11 species from open and forest habitats at three elevational zones of an Andean Mountain. The BT had a positive and significant relationship with environmental temperatures and showed a higher thermal dependence for substrate than air temperature, which suggests that anurans are thermoconformers and potentially tigmotherms. Additionally, BT of anurans from both habitats decreased with the elevation, but species from open habitats had a higher BT than forest species. Therefore, the impact of environmental temperatures on anurans that live at a similar altitude level is not the same, as the type of habitat has a strong influence on their BT. This information is important to a better understanding of anuran thermal biology, refine conservation strategies, and to improve the predictive power of environmental data in forecasting the effects of climate change on small ectotherms such as amphibians.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140740689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Drivers Impacting Carbon Stock and Carbon Offset in a Large-Scale Rubber Plantation in the Middle South of Thailand","authors":"R. Chiarawipa, B. Somboonsuke, Sirima Wandao, Apichet Thongsong, Supet Jirakajohnkool","doi":"10.21315/tlsr2024.35.1.8","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.8","url":null,"abstract":"A large-scale rubber plantation in Southern Thailand is expected to capture a significant amount of carbon dioxide from emissions through carbon sinks in the vegetation and soil. The goal of this research is to create a carbon offset assessment for rubber plantations lasting for 30 years using a voluntary market contract approach. To evaluate the area of large-scale rubber plantations, this study evaluated major growing regions in five provinces in the middle-south region of Thailand (Nakhon Si Thammarat, Phatthalung, Songkhla, Satun and Trang) using an integrated RS-GIS technique that incorporated biomass allometric equations, soil series databases, and object-based classification. The classification of rubber plantation areas and the mapping of rubber stand ages were conducted to estimate the above-ground biomass of the rubber tree. Texture analysis was used in the rubber classification process, and NDVI was combined with texture analysis to separate vegetation areas from other land cover. Four groups of varying ages (1–6, 7–13, 14–20 and 21–30 years old) were evaluated for their capacity to generate carbon offsets. The equations of voluntary market contract revenue according to the contract method of the CCX were applied for this case study. This evaluation was used to estimate their annual value, total and net incomes in the carbon market price regarding the RGGI Allowance (RGA). Carbon offset income was then used to estimate the potential income (over a 30-year period) of the life of the contract. The results showed that the carbon stock potential of rubber plantations depended on the age of the trees and the soil carbon stock. The total carbon stock in the rubber plantations varied from 249.73 to 301.48 Mg C/ha (or equivalently 916.49 to 1,106.44 Mg CO2e/ha). Furthermore, the potential net income of the contract was estimated to be between USD5,378.32 and USD5,930.38 Mg CO2e/ha over a 30-year period according to the voluntary market contract revenue. These results suggest that the large agricultural land plot policy could create opportunities for carbon offsetting. The policy of large-scale rubber areas could be used as a tool and mechanism for farmers who are considering participating in carbon-crediting mechanisms. Then, farmers could use voluntary market contracts as a guide and foundation for their decision-making. The carbon offset credit strategy could assist Thailand in achieving its climate goals of transitioning to a low-carbon agriculture sector.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Characterisation of Three Populations of Heterobranchus longifilis from Nigeria","authors":"Alih Raphael, Olufeagba Solomon Shola Gabriel, Samuel Olabode, O. CheikyulaJoseph, A. A. Bolong, M. Ikhwanuddin, O. V. Tosin, Olufeagba Samuel Olabode, Kiraan Meristik, Keplastikan Morfologi, Ikan Keli, Populasi","doi":"10.21315/tlsr2024.35.1.9","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.9","url":null,"abstract":"This study attempted to discriminate the population of Heterobranchus longifilis in Nigeria using their morphological characteristics. Therefore, 60 sexually mature wild samples of H. longifilis (1:1 for the male and female ratio) of relatively similar size (40 cm) were collected from three eco-regions namely, Guinea Savanna (Benue River, Makurdi), Rainforest Savanna (Niger River, Onitsha) and Sahel Savanna (Rima River, Sokoto). They were transported to the hatchery unit of the Fisheries and Aquaculture Department, Joseph Sarwan Tarka University Makurdi where the morphometric data was collected. The data for 39 traditional morphometric measurements and 5 meristic counts obtained from each fish were subjected to univariate and multivariate analysis. While significant differences were observed in some parameters following univariate analysis; it was revealed that the morphometric parameters and meristic counts could not separate the fish from the different ecoregions into distinct multivariate spaces or clusters following Principal Component Analysis. Hence, this suggests that morphological parameters cannot be used to discriminate H. longifilis from the different ecoregions. Studies using molecular markers are needed to further characterise the distinctiveness of the different populations. ","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous Cytokinin Induces Callus and Protocorm-Like-Bodies (PLBs) Formation in In Vitro Root Tips of Vanilla planifolia Andrews","authors":"Li Chin, Peter G Chai, Alderson Chiew Foan, Chin, Li Chin Chai, Peter G. Alderson, Chiew Foan Chin","doi":"10.21315/tlsr2024.35.1.13","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.13","url":null,"abstract":"Vanilla is a popular flavouring essence derived from the pods of vanilla orchid plants. Due to the high demand for vanilla flavour, high yielding vanilla plantlets are necessary for establishing vanilla plantations. Clonal micropropagation is a viable technique for the mass production of high yielding vanilla plantlets. This study reports an efficient regeneration protocol by using cytokinin as the sole plant growth regulator to regenerate plantlets from the root tips of a commercial vanilla orchid species, Vanilla planifolia. Most studies to date have reported using seeds and nodes as starting explants for in vitro micropropagation of vanilla orchids. So far, regeneration from roots has not been very successful. Previous studies favoured the use of auxins only or high auxin to cytokinin ratios to induce callus, and sole cytokinins were used for direct shoot regeneration. However, it was sporadically observed in plantlets regeneration of V. planifolia that multiple shoots were regenerated from the tips of intact aerial roots submerged in media. This study therefore investigated the regeneration of excised vanilla root tips through the application of most commonly used auxins (1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid) and cytokinins (6-benzylaminopurine and thidiazuron). High auxin presence is known to promote callusing in in vitro plants. However, in this study, auxin treatment inhibits callusing in root tips. While cytokinin treatments, even at low levels, has promoted high rate of callusing. These callus cells regenerate into PLB shoots when cytokinin levels are increased to 0.5 mg/mL BAP under light conditions. The findings of the study have the potential of providing large quantity of high yielding vanilla plantlets through clonal micropropagation. ","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of Cadmium Stress in Rice Seedlings Inoculated with <i>Enterobacter tabaci</i> 4M9 (CCB-MBL 5004).","authors":"Saidu Abdullahi, Hazzeman Haris, Kamarul Zaman Zarkasi, Amir Hamzah Ghazali","doi":"10.21315/tlsr2024.35.1.6","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.6","url":null,"abstract":"<p><p>The growth of crop plants is greatly affected by the increased toxicity of metals. Luckily, certain beneficial bacteria can potentially reduce the effects of metal stress and promote the growth of the host plants. Many species of bacteria were reported as heavy metal tolerant and plant growth promoting, with very little or no report available concerning <i>Enterobacter tabaci</i> as heavy metal tolerant plant growth promoting. The present study aimed to evaluate the potential of Cadmium (Cd) tolerant <i>Enterobacter tabaci</i> 4M9 (CCB-MBL 5004) to alleviate heavy metals stress and enhance the growth of rice seedlings grown under Cd stress conditions. Rice seedlings were grown in Yoshida medium supplemented with different concentrations of Cd and inoculated with 4M9. The results showed that the inoculum tested successfully reduced oxidative stress in the seedlings by reducing the electrolyte leakage (EL) and increasing catalase (CAT) and superoxide dismutase (SOD) activities in the inoculated seedlings compared to the control counterparts. The results also revealed a significant increase in plant growth, biomass, and chlorophyll content of inoculated rice seedlings compared to the control. In general, the Cd tolerant <i>E. tabaci</i> 4M9 confers heavy metal alleviation and thereby improves the growth and survival of rice seedlings under Cd stress conditions. Therefore, the findings stated the potential of 4M9 for alleviating heavy metal stress and promoting the development of inoculated rice seedlings if accidentally grown under Cd-contaminated conditions.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Web-Building Spiders in Gua Kelam, Perlis State Park, Malaysia.","authors":"Johan Ariff Mohtar, Khadijah Hanim Abdul Rahman, Saktheswaran Nyanasilan, Nurul Ain Harmiza Abdullah, Fadhilah Mohamad","doi":"10.21315/tlsr2024.35.1.5","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.5","url":null,"abstract":"<p><p>A cave represents a subterranean ecosystem that harbours a myriad of unique, peculiar, and secluded flora and fauna. These biotas have evolved with a wide range of ecological adaptations that allow them to thrive in harsh environments with limited light. Gua Kelam 1 constitutes part of the Gua Kelam limestone caves system in the Nakawan Range of Perlis State Park, Malaysia. Previous observations indicated that it harbours a plethora of spider species; however, their existence is still elusive as speleobiological studies remain unexplored. Herein, we identified the cavernicolous spiders found in the dark zone areas of Gua Kelam 1 through a complementary approach based on morphology and DNA barcoding. From the morphological analysis, we described three web-building spiders of JTKK2 and JTKK3 groups down to the species-level to belong to <i>Nephilengys malabarensis</i>, and <i>Orsinome vethi</i> except for <i>Pholcus</i> sp. from JTKK4 individuals. The molecular analysis of the cytochrome oxidase-I (COI) genes of JTKK2 and JTKK3 individuals showed that they exhibited a high degree similarity with <i>N. malabarensis</i> (98.3%), and <i>O. vethi</i> (100.0%), respectively except for JTKK4 individuals with only 91.4% homology with <i>P. kuhapimuk</i>. Phylogenetic analysis also generated a congruent tree, in which the identified species are well nested within the family Araneidae, Tetragnathidae, and Pholcidae. By this integral approach, the three spiders were determined as <i>N. malabarensis</i>, <i>O. vethi</i>, and <i>Pholcus</i> sp. These spiders are originally epigean in their habitat but uniquely thrive in Gua Kelam 1.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Bacteria Induce Thiamine (Vitamin B1) Production in Oil Palm (<i>Elaeis guineensis</i>).","authors":"Nur Asna Faiqah Johari, Aisamuddin Ardi Zainal Abidin, Nur Farhah Nabihan Ismail, Zetty Norhana Balia Yusof","doi":"10.21315/tlsr2024.35.1.1","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.1","url":null,"abstract":"<p><p>Thiamine or vitamin B1 is a micronutrient that has a crucial function in all living organisms and involved in several biochemical reactions. Concerning the capability of thiamine in inducing plant health, a study was carried out by applying bacterial endophytes (<i>Pseudomonas aeruginosa</i> and <i>Burkholderia cepacia</i> cultures) in four-month-old oil palm seedlings (<i>Elaeis guineensis</i>) via soil drenching technique to evaluate the effect towards thiamine. Spear leaves were sampled day 0 to 14 to analyse the expression of gene coding for the first two enzymes thiamine biosynthesis pathway, THI4 and THIC via qPCR analysis. The gene expression by qPCR showed a significant increase of up to 3-fold while high-performance liquid chromatography (HPLC) analysis for quantification of thiamine and its derivatives accumulated ~ 20-fold in total thiamine when compared to control seedlings. However, concentration of thiamine metabolites was negatively correlated with the expression of THIC and THI4 gene transcripts suggesting post-transcriptional regulation mediated by an RNA regulatory element, a thiamine pyrophosphate (TPP) riboswitch. Our findings demonstrated that the application of bacterial endophytes affected thiamine biosynthesis and enhanced overall thiamine content. This might increase the plant's resistance towards stress and would be useful in oil palm maintenance for maximum yield production.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In silico</i> EST-SSR Identification and Development through EST Sequences from <i>Metroxylon sagu</i> Rottb. for Genetic Diversity Analysis.","authors":"Devit Purwoko, Siti Zulaeha, Teuku Tajuddin, Farida Rosana Mira, Maharani Dewi Solikhah, Gemilang Rahmadara, Nurul Fitri Hanifah, Rusmanto","doi":"10.21315/tlsr2024.35.1.2","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.2","url":null,"abstract":"<p><p>Sago plant (<i>Metroxylon sagu</i> Rottb.) is one of the most carbohydrate-producing plants in the world. Microsatellites or simple sequence repeats (SSRs) play an important role in the genome and are used extensively compared to other molecular markers. For the first time, we are exploiting data expressed sequence tags (EST) of sago plants to identify and characterise markers in this species. EST data about sago plants are obtained through the EST database on the National Center for Biotechnology Information (NCBI) website. We obtained data of 458 Kb (412 contig) with a maximum and minimum length of 1,138 and 124 nucleotides, respectively. We successfully identified 820 perfectly patterned SSR using Phobos 3.3.12 software. The type characterisation of EST-SSR was dominated by tri-nucleotides 36% (294), followed by hexa-nucleotides 24% (202), tetra-nucleotides 15% (120), penta-nucleotides 13% (108) and di-nucleotides 12% (96). The most frequency of SSR motifs in each type is AG, AAG and AAAG. Analysis of synteny on the EST sequence with the online application Phytozome found that sequences were distributed on 12 <i>Oryza sativa</i> chromosomes with a likeness percentage between 63% to 100% and e-value between 0 to 0.094. We developed the primer and generated 19 primers. Furthermore, we validated 7 primers that all generated polymorphic alleles. To our knowledge, this report is the first identification and characterisation of EST-SSR for sago species and these markers can be used for genetic diversity analysis, marker assisted selection (MAS), cultivar identification, kinship analysis and genetic mapping analysis.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity, Encounter Rate and Detection of Non-Volant Nocturnal Mammals on Two Malaysian Islands.","authors":"Priscillia Miard, Foo Kai Xin, Sapphire Hampshire, Nik Fadzly Nik Rosely, Henry Bernard, Nadine Ruppert","doi":"10.21315/tlsr2024.35.1.4","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.4","url":null,"abstract":"<p><p>Nocturnal mammals constitute a crucial component of tropical faunal diversity, but not much is known about the effects of anthropogenic disturbance on the habitat use and detectability of these species. We investigated which habitat and environmental variables impact the detectability of non-volant nocturnal arboreal mammals across varying habitat types at two tropical islands with different levels of anthropogenic development in Malaysia. We conducted night transect line and point count surveys following pre-existing paths in Penang Island and Langkawi Island between 2019 and 2020. We used a head torch with red filter and a thermal imaging device (FLIR) to enhance animal detection success. We calculated the encounter rates (individual km^-1) for each species as a proxy for abundance. Overall, we detected 17 species, but did not find higher species diversity in intact forested environments compared to disturbed areas. Encounter rates of the most observed species were influenced by 'time after sunset' on the highly developed island of Penang, whereas on the rural island of Langkawi, detection was higher in sites with better canopy connectivity. Different species of non-volant nocturnal arboreal mammals use their respective habitats differently and thus, are differently impacted by varying levels of anthropogenic activities. Our results provided baseline data on the diversity, encounter rate, and detectability of these highly elusive species, which can also help to further improve methodologies for the detection of nocturnal wildlife.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agro-Industrial Wastes as Potential Substrates for Rhamnolipid Production by <i>Pseudomonas aeruginosa</i> USM-AR2.","authors":"Mohd Shafiq Nasir, Ahmad Ramli Mohd Yahya, Nur Asshifa Md Noh","doi":"10.21315/tlsr2024.35.1.3","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.3","url":null,"abstract":"<p><p>Rhamnolipid has gained much attention in various fields owing to its distinctive functional properties compared to conventional chemical surfactants, which are mostly derived from petroleum feedstock. Production cost is one of the main challenges in rhamnolipid production, particularly when using refined substrates. One possible solution is to use agro-industrial wastes as substrates for rhamnolipid production. This is a promising strategy due to their abundance and commercially low value, while simultaneously alleviating an agro-industrial waste management problem in the environment. This study aims to evaluate agro-industrial wastes from local crops as possible low-cost alternative substrates for rhamnolipid production by a local isolate, <i>Pseudomonas aeruginosa</i> USM-AR2. Various liquid wastes, namely sugarcane molasses, rice washing water, overly mature coconut (OMC) water, empty fruit bunch (EFB) steam effluent, palm sludge oil (PSO) and palm oil mill effluent (POME) were screened as the main carbon source supplementing mineral salt medium (MSM) in the fermentation of <i>P. aeruginosa</i> USM-AR2. Batch fermentation was carried out in a shake flask system, agitated at 200 rpm and incubated at room temperature, 27 ± 2°C for 120 h. Among the substrates tested, PSO exhibited the highest biomass at 20.78 g/L and rhamnolipid production at 1.07 g/L. This study has shown the potential of agro-industrial wastes in Malaysia as an alternative resource for rhamnolipid production, transforming them into value added products, while reducing the amount of wastes discharged into the environment.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}