Tropical Journal of Pharmaceutical Research最新文献_第4页

Synergistic effect of continuous care and cephalosporin antimicrobials in managing pulmonary infections in acute stroke patients: A comprehensive study 持续护理和头孢菌素抗菌药物在治疗急性脑卒中患者肺部感染中的协同作用:一项综合研究

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI: 10.4314/tjpr.v22i10.24

Lanqing W, Lirong Chen, Haiyou Liao

{"title":"Synergistic effect of continuous care and cephalosporin antimicrobials in managing pulmonary infections in acute stroke patients: A comprehensive study","authors":"Lanqing W, Lirong Chen, Haiyou Liao","doi":"10.4314/tjpr.v22i10.24","DOIUrl":"https://doi.org/10.4314/tjpr.v22i10.24","url":null,"abstract":"Purpose: To investigate potential benefits of continuous nursing combined with cephalosporin antibiotics in patients with acute cerebral infarction complicated by pulmonary infection. Methods: A total of 106 patients diagnosed with acute cerebral infarction complicated by pulmonary infection were selected for this study, and admitted to The Second Affiliated Hospital of Hainan Medical University, Haikou, China from June 2020 to June 2022. Patients were randomly divided into a control group (which received conventional care and cephalosporin antibiotics, n =53), and a study group managed with sustained nursing and cephalosporin antibiotics (n = 53). The study compared various clinical parameters between two groups before and after intervention, including time of symptom relief for fever, cough, and wet rales, as well as the National Institutes of Health Stroke Scale (NIHSS), FuglMeyer Assessment (FMA), Barthel Index (BI), Self-Perceived Burden Scale (SPBS), Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), Acute Physiology and Chronic Health Evaluation II (APACHE II) score, and severity of pulmonary infection which was assessed by CURB 65 score. Results: Compared with control group, study group had a significantly shorter clinical symptom relief time after intervention (p < 0.05). After intervention, NIHSS, SPBS, SAS, SDS, APACHE II, and CURB 65 scores in study group were significantly lower, whereas FMA and BI scores were significantly higher before intervention (p < 0.05).  Conclusion: Administration of nursing care and cephalosporin antibiotics reduces the time required for symptom relief, and improves neurological function in patients with acute cerebral infarction and pulmonary infections.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"88 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135685274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nectin-3 and Nectin-4: potential prognostic biomarkers for therapeutic targeting of cancer Nectin-3和Nectin-4:癌症靶向治疗的潜在预后生物标志物

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI: 10.4314/tjpr.v22i10.27

Aihong Yang, Yan Ge, Panpan Yang, Yuqi Xin, Chenlu Zhang, Feixue Xu, Jiao Yang

引用次数: 0

MiR-22 inhibits angiotensin II-induced aortic dissection and protects aortic vessel wall in mice by targeting MAPK14 MiR-22通过靶向MAPK14抑制血管紧张素ii诱导的小鼠主动脉夹层并保护主动脉壁

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI: 10.4314/tjpr.v22i10.9

Xinming Yu, Zonggang Zhao, Lili Tao, Xiao Shun, Liu Bing

{"title":"MiR-22 inhibits angiotensin II-induced aortic dissection and protects aortic vessel wall in mice by targeting MAPK14","authors":"Xinming Yu, Zonggang Zhao, Lili Tao, Xiao Shun, Liu Bing","doi":"10.4314/tjpr.v22i10.9","DOIUrl":"https://doi.org/10.4314/tjpr.v22i10.9","url":null,"abstract":"Purpose: To study the effect of miR-22 on angiotensin II-induced aortic dissection in mice, and its protective effect on aortic vessel wall, as well the involvement of MAPK-14 in these processes. Methods: A mouse aortic dissection model was established via subcutaneous implantation of angiotensin II (1 µg/kg/min) micropump in the dorsal region. The mice (n = 30) were assigned in equal numbers to 5 groups (n = 6). All injections were given via the tail vein. The miR-22 expressions in aortas of mice in each group were determined with quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Western blot assay was used to determine the expressions of MAPK-14 protein, while H&E staining was used to measure the ratio of aortic thickness-to-diameter, and contents of collagen and elastic fibers. Results: The expression of miR-22 in aorta of mice in miR-22 overexpression group was significantly higher than that in overexpression control group, but significantly lower in miR-22 inhibition mouse than in inhibition control mouse (p < 0.05). There was significantly lower protein expression of MAPK-14 in mice aorta in miR-22 overexpression mice than in overexpression control mice, but significantly upregulated in miR-22 inhibition mice, relative to that in inhibition control mice (p < 0.05). In the miR-22 overexpression mice, the ratio of membrane thickness-to-diameter was higher than the corresponding value in miR-22 inhibition mice. There were significantly higher contents of aortic elastic and collagen fibers in miR-22 overexpression mice than in overexpression control and miR-22 inhibition groups (p < 0.05). Conclusion: Overexpression of miR-22 inhibits the up-regulation of expression of its target gene mapk14, increases thickness of aortic media and aortic elasticity in mice, and increase the content of collagen fibers, thereby exerting protective effect on aortic wall structure.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"89 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135685386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tectorigenin functions as a potential drug for melanoma treatment via inhibition of cell growth, migration and aerobic glycolysis 鸢头黄素通过抑制细胞生长、迁移和有氧糖酵解而成为治疗黑色素瘤的潜在药物

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI: 10.4314/tjpr.v22i10.8

Qiao Yan, Fei Wang

{"title":"Tectorigenin functions as a potential drug for melanoma treatment via inhibition of cell growth, migration and aerobic glycolysis","authors":"Qiao Yan, Fei Wang","doi":"10.4314/tjpr.v22i10.8","DOIUrl":"https://doi.org/10.4314/tjpr.v22i10.8","url":null,"abstract":"Purpose: To investigate the role of tectorigenin (TG) in melanoma cells. Methods: Viability of A375 and A2058 melanoma cells was determined by cell counting kit (CCK-8) assay. Cell cycle progression was analyzed by 5-ethynyl-2’-deoxyuridine (EdU) staining. Migration and invasion of melanoma cells were determined by Transwell assay. The epithelial-mesenchymal transition (EMT) of melanoma cells was investigated by determining expression of E-Cadherin and Snail. Expression of glucose transporter 1 (GLUT1) and lactose dehydrogenase A (LDHA) was assessed via western blotting. Glucose and lactate production was evaluated by corresponding assay kit. The NOX4 and FOXM1 expressions were determined using western blotting.  Results: Tectorigenin inhibited proliferation, migration and invasion of A375 and A2058 melanoma cells. Tectorigenin regulated cell cycle progression by decreasing the number of cells in S-phase and significantly inhibited snail expression and increased expression of E-Cadherin (p < 0.05), thus inhibiting the EMT of A375 and A2058 cells. Tectorigenin inhibited expression of GLUT1 and LDHA, thereby leading to reduction in glucose consumption and lactate production. It also significantly inhibited expressions of NOX4 and FOXM1 (p < 0.05), indicating an inhibitory effect on the activity of NOX4/FOXM1 pathway. Conclusion: Tectorigenin inhibits aerobic glycolysis, growth and migration in melanoma cells by suppressing the activity of NOX4/FOXM1 pathway, suggesting its potential in melanoma treatment.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"89 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135685387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary study on the relationship between recurrence and quasispecies characteristics in P region of hepatitis B virus genome of chronic hepatitis B patients treated with lamivudine 拉米夫定治疗慢性乙型肝炎患者乙肝病毒基因组P区准种特征与复发关系的初步研究

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI: 10.4314/tjpr.v22i10.21

Baojian Wang, Bobin Hu, Jianning Jiang, Minghua Su, Xiaoli Wu, Shaohua Zhong, Yanxiu Liang, Shihua Li, Rong Xie

{"title":"Preliminary study on the relationship between recurrence and quasispecies characteristics in P region of hepatitis B virus genome of chronic hepatitis B patients treated with lamivudine","authors":"Baojian Wang, Bobin Hu, Jianning Jiang, Minghua Su, Xiaoli Wu, Shaohua Zhong, Yanxiu Liang, Shihua Li, Rong Xie","doi":"10.4314/tjpr.v22i10.21","DOIUrl":"https://doi.org/10.4314/tjpr.v22i10.21","url":null,"abstract":"Purpose: To investigate the characteristics of quasispecies in the P region of hepatitis B viral (HBV) DNA of chronic hepatitis B (CHB) patients treated with lamivudine (LAM), and its effect on HBV relapse after drug withdrawal in CHB patients who met drug cessation criteria. Methods: A total of 43 patients with chronic HBV infection, who had undergone LAM therapy, were enrolled in this study. Treatment was discontinued for patients who met therapeutic criteria set by relevant Asian-Pacific regions. Polymerase chain reaction (PCR) was used to amplify the genome in P region of serum rcDNA before treatment, cccDNA during drug cessation period, and serum rcDNA at relapse. Quasispecies cloning and sequencing were performed to identify variable sites in HBV P region. Results: Mutations in P region of baseline serum rcDNA were detected in 30 CHB patients, with N/H238T (14/30), L/F/Q/R267H (12/30), V278T (12/30), D134E/I (11/30), and T222A (9/30) having highest rates. In hepatocellular cccDNA P region during drug withdrawal, most detectable mutations were L/F/Q/R267H (25/43), V278T (18/43), N/H238T (15/43), D134E/I (14/43), and T222A (11/43). During relapse, the highest detectable mutation rates in serum rcDNA P region were N/H238T (12/19), L/F/Q/R267H (10/19), T222A (10/19), and V278T (8/19). Conclusion: High mutation rates of T222A and N/H238T in P region of HBV DNA increase the risk of relapse in patients. As a result, patients are susceptible to relapse after drug withdrawal.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"88 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135685389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined sevoflurane/sufentanil anesthesia for removal of tracheal foreign bodies in pediatric surgery, and its effect on hemodynamics 七氟醚/舒芬太尼联合麻醉在小儿外科气管异物取出中的应用及其对血流动力学的影响

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.18

Huaqu Gong, Tingting Liu, Lirong Duan, Lirong Duan, Haiyang Wang

{"title":"Combined sevoflurane/sufentanil anesthesia for removal of tracheal foreign bodies in pediatric surgery, and its effect on hemodynamics","authors":"Huaqu Gong, Tingting Liu, Lirong Duan, Lirong Duan, Haiyang Wang","doi":"10.4314/tjpr.v22i9.18","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.18","url":null,"abstract":"Purpose: To determine the efficacy of sevoflurane + sufentanil combination anesthesia in pediatric surgery for removal of tracheal foreign bodies, and its effect on hemodynamics.Methods: A total of 128 children with airway foreign bodies were assigned to control and study groups, each with 64 children. The control patients received slow intravenous injection of propofol (3 mg/kg). The study group was given 5 % sevoflurane and a slow intravenous injection of sufentanil (0.3 μg/kg). Hemodynamic parameters (diastolic blood pressure, systolic blood pressure and heart rate) were recorded before induction of anesthesia, at the time of intubation, during placement of a rigid bronchoscope (when the lens was placed), during removal of foreign bodies, and when extubating. Complications in children in the two groups after the removal of airway foreign body were recorded.Results: At the times of intubation and extubation, blood pressure and heart rate were significantly increased in both groups, but appreciably lower values were seen in study group. The major complications in pediatric airway foreign body removal in two groups were vomiting, bronchospasm and holding of breath.Conclusion: In children with airway extraction, sevoflurane, in combination with sufentanil produced better anesthetic effect and less impact on hemodynamics than propofol. Moreover, the children woke up faster and had fewer complications. The combined anesthesia is safe and reliable. However, the clinical application of this combined anesthesia requires larger-sample clinical studies.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ruscogenin regulates endogenous antioxidation in dopamine neurons by activating Keap1/Nrf2/HO-1 pathway Ruscogenin通过激活Keap1/Nrf2/HO-1通路调控多巴胺神经元内源性抗氧化

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.3

Kai Shi, Bing Wang

{"title":"Ruscogenin regulates endogenous antioxidation in dopamine neurons by activating Keap1/Nrf2/HO-1 pathway","authors":"Kai Shi, Bing Wang","doi":"10.4314/tjpr.v22i9.3","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.3","url":null,"abstract":"Purpose: To investigate the effect of ruscogenin (RUS) on cell viability, lipid peroxidation and mitochondrial dysfunction in a Parkinson’s disease (PD) model.Methods: The neuroblastoma cell line SH-SY5Y was modified with 1-methyl-4-phenylpyridine (MPP+) to establish a PD model. RUS (1 or 10 μM) was used to treat MPP+ induced SH-SY5Y cells. Cell viability was assessed using CCK-8 assay. The concentrations of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GPx) were determined by enzyme-linked immunosorbent assay (ELISA). ATP production, Ca2+ concentration and JC-1 were quantified using commercial kits. The expression levels of tyrosine hydroxylase (TH), Keap1, Nrf2 and HO-1 were evaluated by western blot analysis.Results: RUS protected cell viability, reduced LDH production, and elevated TH expression in MPP+- modified SH-SY5Y cells. RUS promoted the release of SOD, CAT and GPx, but suppressed MDA production. Furthermore, RUS enhanced ATP metabolism, decreased Ca2+ leakage and maintained mitochondrial function. RUS also repressed Keap1 expression but increased Nrf2 and HO-1 levels.Conclusion: RUS enhances cell viability while alleviating cytotoxicity, lipid peroxidation and mitochondrial dysfunction in dopamine neurons through the activation of Keap1/Nrf2/HO-1 signaling pathway. Thus, RUS is a promising therapeutic candidate for PD treatment.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"213 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meta-analysis of the effect of losartan relative to other angiotensin receptor antagonists on serum uric acid level 氯沙坦相对于其他血管紧张素受体拮抗剂对血清尿酸水平影响的meta分析

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.31

Jinjie Zhou, Yongwei Zhang, Lin Cheng

{"title":"Meta-analysis of the effect of losartan relative to other angiotensin receptor antagonists on serum uric acid level","authors":"Jinjie Zhou, Yongwei Zhang, Lin Cheng","doi":"10.4314/tjpr.v22i9.31","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.31","url":null,"abstract":"Purpose: To systematically analyze the disparity in uric acid reduction between losartan and other angiotensin receptor antagonists.Methods: A computer-based search was conducted in databases including EMBASE, PubMed, Wanfang, CNKI, Ovid MEDLINE, and Web of Science to identify original literature comparing the impact of losartan with other angiotensin receptor antagonists on blood uric acid levels. Utilizing the Cochrane Collaboration bias risk tool, a quality assessment of the included randomized controlled studies was conducted.Results: A total of 12 publications were obtained, consisting of 6 randomized controlled studies and 6 cohort studies. Five of the twelve publications assessed the impact of losartan compared to valsartan on blood uric acid levels. The heterogeneity analysis yielded I2 = 98 % (p < 0.01), indicating substantial variability among the studies. Findings indicated that losartan was more effective than valsartan in reducing blood uric acid levels (SMD = -3.26, 95 % CI (-5.01 to 1.51), p < 0.05). Four publications investigated the impact of losartan versus telmisartan on blood uric acid levels. The results revealed that losartan had a greater effect in reducing blood uric acid levels compared to telmisartan (SMD = -1.77, 95 % CI (-3.411 to -0.13), p < 0.05).Conclusion: Among the angiotensin receptor antagonists used as antihypertensive drugs, losartan stands out for its significant ability to reduce uric acid levels. This finding provides a strong evidencebase for making clinical medication decisions.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FXR1 knockdown inhibits the malignant behavior of colorectal cancer by suppressing epithelial-tomesenchymal transition FXR1敲低通过抑制上皮-间质转化抑制结直肠癌的恶性行为

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.1

Qindan Du, Jiayao Chen, Honglei Li, Yixin Bian, Xiaoying Wang, Chen YQ, Xiaosong Ge

{"title":"FXR1 knockdown inhibits the malignant behavior of colorectal cancer by suppressing epithelial-tomesenchymal transition","authors":"Qindan Du, Jiayao Chen, Honglei Li, Yixin Bian, Xiaoying Wang, Chen YQ, Xiaosong Ge","doi":"10.4314/tjpr.v22i9.1","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.1","url":null,"abstract":"Purpose: To determine the role of Fragile X-related protein-1 (FXR1) in colon cancer progression and its relationship to patients’ survival.Methods: A total of 164 colorectal cancer (CRC) patients, admitted to the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China between 2006 and 2008, were included in this study. Immunohistochemistry was used to semi-quantitatively analyze the intensity and extent of immunological staining of diaminobenzidine-stained paraffin blocks of CRC samples. The study also retrieved COAD mRNA and patients’ clinical data from TCGA and cultured human colon cancer cell lines (SW480, SW620, HCT8, HCT116, and Caco2) in RPMI 1640 medium to assess the propensity of CRC cells to proliferate, invade the tumorigenicity in BALB/c nude mice.Results: The prognosis of CRC patients was inversely linked with the expression of FXR1. Additionally, FXR1 knockdown in CRC cells reduced cellular growth, colony development and tumorigenesis. After presenting BALB/c nude mice with tumors in FXR1 knockdown, the cells displayed higher E-cadherin levels (p < 0.01) as well as decreased TGF-1 (p < 0.01) and N-cadherin levels (p < 0.001).Conclusion: Fragile X-related protein-1 is an oncogene in colon cancer and its knockdown inhibits HCT116 cells from behaving malignantly. Thus, FXR1 is a potential treatment option for CRC.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGF-β promotes proliferation and inhibits apoptosis of liver cancer Huh-7 cells by regulating MiR-182/CADM1 TGF-β通过调控MiR-182/CADM1促进肝癌Huh-7细胞增殖，抑制凋亡

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.4

An Wang, Yucheng Huang, Xiaoping Yang

{"title":"TGF-β promotes proliferation and inhibits apoptosis of liver cancer Huh-7 cells by regulating MiR-182/CADM1","authors":"An Wang, Yucheng Huang, Xiaoping Yang","doi":"10.4314/tjpr.v22i9.4","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.4","url":null,"abstract":"Purpose: To investigate the mechanism of liver cancer cell tolerance to the antiproliferative effect of transforming growth factor beta (TGF-β) based on miRNA levels.Methods: MiRNA microarray and quantitative reverse transcription-polymerase chain reaction (qRTPCR) were used to identify differentially expressed miRNAs in liver cancer Huh-7 cells treated with TGF-β. The effect of these miRNAs on patient survival was analyzed using Kaplan-Meier Plotter. Involvement of Smad2/Smad3 in TGF-β-induced miR-182 expression was determined using shRNA knockdown and qRT-PCR. Dose-dependent effect of TGF-β on miR-182 expression was investigated in Huh-7 cells and mouse primary liver cells using qRT-PCR. The effect of miR-182 on Huh-7 cell proliferation and apoptosis was studied using CFSE and Annexin V/PI assay. Direct targets of miR-182 in Huh-7 cells were identified using a luciferase reporter gene assay, while the influence of Recombinant Cell Adhesion Molecule 1 (CADM1) overexpression on Huh-7 cell proliferation and apoptosis treated with miR-182 was examined using lentivirus experiments, and CFSE and Annexin V/PI assays.Results: The expression levels of hsa-miR-181a, hsa-miR-182, hsa-miR-483, and hsa-miR-143 were significantly higher in serum samples from liver cancer patients (p < 0.05). Survival analysis showed that low expression of hsa-miR-182 and high expression of hsa-miR-483 increased the survival rate of liver cancer patients. Furthermore, TGF-β increased miR-182 expression in Huh-7 cells, but not in mouse primary liver cancer cells. The MiR-182 promoted Huh-7 cell proliferation and inhibited apoptosis. It targeted CADM1 mRNA 3'-UTR, decreasing CADM1 expression. Overexpression of MiR-182 significantly reduced cell proliferation and increased apoptosis in Huh-7 cells with CADM1 overexpression (p < 0.05).Conclusion: Transforming growth factor beta (TGF-β) facilitates the proliferation and repression of apoptosis of Huh-7 cells by increasing miR-182 expression and inhibiting CADMI expression.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0