Tropical Journal of Pharmaceutical Research最新文献_第5页

MAPK1 knockdown ameliorated immune and inflammatory abnormalities in a mouse model of refractory asthma MAPK1敲低可改善难治性哮喘小鼠模型的免疫和炎症异常

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.9

Shuang Lin, Xiaohong Yang

{"title":"MAPK1 knockdown ameliorated immune and inflammatory abnormalities in a mouse model of refractory asthma","authors":"Shuang Lin, Xiaohong Yang","doi":"10.4314/tjpr.v22i9.9","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.9","url":null,"abstract":"Purpose: To evaluate the potential molecular mechanisms involved in refractory asthma in an animal model, and the potential therapeutic effect of MAPK1 knockdown on the disease.Methods: Eighteen female Institute of Cancer Research (ICR) mice, aged 8 - 10 weeks, were randomly divided into three groups: control, asthma model and refractory asthma, with 6 mice in each group. The expression of MAPK1 was knocked down in mice using an adenoviral vector. Subsequently, the methylation levels of MAPK1 promoter in mouse lung tissue were determined using methylation assays. Hematoxylin and eosin (H&E) staining and Periodic Acid-Schiff (PAS) staining were used to determine inflammatory and histological changes in lung tissues. Levels of immune cells were determined using flow cytometry, while Western blotting was used to measure the protein expression levels of ERK1/2, JNK, MEK1/2 and p38.Results: Methylation assay results show that mean methylation level of cg11335969 locus was significantly reduced in the refractory asthma mouse model (p < 0.05). The levels of IgG1 and IgM in refractory asthmatic mice were reduced after MAPK1 knockdown. There was a significantly reduced degree of lung lesions in mice (p < 0.05), as was reflected in effectively decreased histopathological changes. Protein levels of ERK1/2, JNK, MEK1/2 and p38, and the levels of neutrophils, dendritic cells, and macrophages were significantly decreased (p < 0.05).Conclusion: There is hypermethylated modification of MAPK1 at cg11335969 site in refractory asthma mouse model. Knockdown of MAPK1 attenuates inflammation and tissue damage, and reverses abnormal immune cell numbers in refractory asthma mice. Thus, MAPK1 inhibition may be a novel strategy for ameliorating immune abnormalities in refractory asthma.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between breast cancer and osteocalcin levels, and risk of hypertension in postmenopausal women: Implications for pharmacological intervention 绝经后妇女乳腺癌与骨钙素水平和高血压风险的相关性:药物干预的意义

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.14

Qianqian Cai, Mingyue Xu, Xueyan Zheng, Qiaoyun Zhu, Xiaodan Wu

{"title":"Correlation between breast cancer and osteocalcin levels, and risk of hypertension in postmenopausal women: Implications for pharmacological intervention","authors":"Qianqian Cai, Mingyue Xu, Xueyan Zheng, Qiaoyun Zhu, Xiaodan Wu","doi":"10.4314/tjpr.v22i9.14","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.14","url":null,"abstract":"Purpose: To investigate the correlation between breast cancer (BC), osteocalcin (OC) and hypertension risk in postmenopausal women.Methods: Two hundred patients with BC and 200 non–BC women who visited The First People's Hospital of Wenling Hospital, Wenling, China from June 2018 to March 2021 were included in the study. Fifty-three patients with hypertension and 157 non-hypertensive patients were included in the hypertension studies. The enrolled postmenopausal women were divided into control, BC, hypertensive and non–hypertensive groups and risk factors for hypertension were analyzed. Pearson's correlation coefficient was performed to correlate OC levels with clinical indices. The incidence of hypertension in each group was counted and its correlation with OC levels was determined using Chi–square test.Results: Age, BMI, OC, SBP, DBP, TC, TG, PTH and FPG levels were risk factors for the progression of hypertension in postmenopausal women (OR > 1, p < 0.05). Serum levels of OC in postmenopausal women had negative correlation with age, BMI, SBP, DBP, TC, TG, PTH, 25–(OH)D3 and FPG levels (r < 0, p < 0.05), but had positive correlation with HDL–C, CTX and PINP levels (r > 0, p < 0.05). With the elevation of OC levels, the incidence of hypertension showed a decreasing trend in postmenopausal women (p < 0.05).Conclusion: Menopause is a risk factor for BC in women, while low OC levels are significantly related to elevated risk of hypertension in postmenopausal women. These findings suggest that armacological intervention to increase OC levels may have potential benefits in mitigating the risk of hypertension in postmenopausal women.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Tiaoxie Yizhi Formula combined with atomoxetine and biofeedback therapy of school-age children with attention deficit hyperactivity disorder 调泻益智方联合托莫西汀及生物反馈治疗学龄期儿童注意缺陷多动障碍的疗效

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.23

Wanyan Yao, Jing Cai, Guanjun Liang, Mingding Li, Min Su

{"title":"Effect of Tiaoxie Yizhi Formula combined with atomoxetine and biofeedback therapy of school-age children with attention deficit hyperactivity disorder","authors":"Wanyan Yao, Jing Cai, Guanjun Liang, Mingding Li, Min Su","doi":"10.4314/tjpr.v22i9.23","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.23","url":null,"abstract":"Purpose: To investigate the effect of Tiaoxie Yizhi Formula combined with atomoxetine and biofeedback therapy on the Swanson, Nolan, and Pelham-IV Rating Scale (SNAP-IV) score as well as Clinical Global Impression (CGI) score of school-age children with attention deficit hyperactivity disorder (ADHD).Methods: Clinical data of 85 school-age children with ADHD who were admitted to Dushu Lake Hospital from April 2019 to June 2022 were analyzed retrospectively. Patients treated with biofeedback therapy and atomoxetine for 1 month were defined as control group (n = 42), while those treated with Tiaoxie Yizhi Formula in addition to what was given control group were defined as study group (n = 43). Efficacy, Traditional Chinese Medicine (TCM) symptom scores, SNAP-IV score, CGI score and cerebral electrophysiological indices were compared between the groups. The scores of the main and secondary syndromes were also compared between two groups.Results: Treatment response rate in study group was significantly higher than in control group (p < 0.05). There was no significant difference in scores of the main syndromes and secondary syndromes, SNAP-IV score or CGI score between the two groups before treatment (p > 0.05). However, scores of the two groups decreased significantly after treatment, and study group had significantly lower scores than control group (p < 0.05). There was no significant difference in sensorimotor rhythms (SMR) waves, β waves, or θ waves between the two groups before and after treatment (p > 0.05).Conclusion: Tiaoxie Yizhi Formula plus atomoxetine plus biofeedback therapy improves ADHDsymptoms more tha n biofeedback therapy and atomoxetine combination as shown by the reduction in TCM, SNAP-IV and CGI scores. However, investigations will need to be carried out using a larger sample size in order to validate these findings.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship among serum TIMP-1, sCD40L and peripheral neuropathy in type 2 diabetes 2型糖尿病患者血清TIMP-1、sCD40L与周围神经病变的关系

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.24

Zhang Yong, Bai Feng

{"title":"Relationship among serum TIMP-1, sCD40L and peripheral neuropathy in type 2 diabetes","authors":"Zhang Yong, Bai Feng","doi":"10.4314/tjpr.v22i9.24","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.24","url":null,"abstract":"Purpose: To determine the correlation of serum TIMP-1 and sCD40L with peripheral neuropathy in type 2 diabetes (T2DM), and potential targets for pharmacological intervention.Methods: The study enrolled 97 subjects with T2DM. The 41 subjects with simple diabetes were classified as T2DM group, while 56 subjects with diabetic peripheral neuropathy (DPN) were included in the DPN group. A control group consisting of 50 healthy volunteers who conducted health checks during a similar time-period was also included. Clinical data and parameters were compared among the three groups, and TIMP-1 and sCD40L levels were determined. Univariate and multivariate logistic regression analyses were conducted to identify factors affecting DPN. The diagnostic value of TIMP-1 and sCD40L in DPN was also determined.Results: Body mass index (BMI) varied among the three groups (p < 0.05), but age or gender were not significantly different (p < 0.05). Duration of diabetes was longer in DPN group than in T2DM group (p < 0.05). The study also revealed statistically significant differences in fasting blood glucose (FBS), postprandial blood glucose, and glycosylated hemoglobin among the three groups. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and soluble CD40 ligand (sCD40L) were important factors that affected the occurrence of diabetic peripheral neuropathy (p < 0.05).Conclusion: TIMP-1 and sCD40L levels reflect neurovascular injury in patients with diabetes, with potential as targets for pharmacological intervention for peripheral neuropathy. These findings espouse crucial clinical implications for early identification and treatment in type 2 diabetes.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dostarlimab: Novel paradigm shift in cancer therapy dostarlimumab:癌症治疗的新范式转变

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.32

Ali Alquraini

{"title":"Dostarlimab: Novel paradigm shift in cancer therapy","authors":"Ali Alquraini","doi":"10.4314/tjpr.v22i9.32","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.32","url":null,"abstract":"Cancer is one of the leading causes of death worldwide. The rising global death from cancer requires novel approaches for treating various types of cancer. Numerous malignancies are now being treated more effectively by immunotherapy. Dostarlimab is an example of an immune checkpoint inhibitor (ICI) that works by blocking the programmed cell death protein-1 receptor (PD-1). Dostarlimab has shifted the paradigm of treatment of cancer from using conventional therapies to a new, promising approach. This new approach increases the options of therapies and chances to achieve a higher response rate. Dostarlimab is a novel antibody, that prevents the binding of ligands to PD-1 on T-cells. Dostarlimab has demonstrated encouraging outcomes in the treatment of various cancers such as endometrial cancer, rectal cancer, and non–small cell lung cancer (NSCLC). The cure rate with dostarlimab in some types of cancer, such as rectal cancer, is 100 %. This review presents a recent understanding of the use of dostarlimab in clinical trials and opens up the doors for clinicians and investigators about future possibilities of using dostarlimab either alone or combined with other anticancer medications to treat various cancers.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of ultrasound-guided subacromial bursa steroid injection for treating shoulder pain in post-stroke hemiparetic patients 超声引导肩峰下囊类固醇注射治疗脑卒中后偏瘫患者肩痛的疗效观察

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.15

Sunbin Chen, Shibin Lin, Minghui Li, Siwei Li, Kefeng Yang

{"title":"Efficacy of ultrasound-guided subacromial bursa steroid injection for treating shoulder pain in post-stroke hemiparetic patients","authors":"Sunbin Chen, Shibin Lin, Minghui Li, Siwei Li, Kefeng Yang","doi":"10.4314/tjpr.v22i9.15","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.15","url":null,"abstract":"Purpose: To assess the efficacy of ultrasound-guided subacromial bursa (SAB) steroid injection for treating hemiplegic shoulder pain (HSP) after stroke (CS), as well as its impact on joint mobility, and inflammatory mediators.Methods: Seventy-two (72) patients with CS-related HSP were divided into two groups: study and control groups. The study group received ultrasound-guided SAB injection of triamcinolone acetonide in addition to conventional treatment, while control group received only conventional rehabilitation. Both groups received 4 weeks of treatment and were assessed using pain visual analogue scale (VAS) and passive range of motion (PROM) indices before treatment and at 1, 4, and 12 weeks after treatment. Additionally, the Fugl-Meyer motor function (FMA-U) scale score, activities of daily living (ADL) score, modified Barthel index (MBI), serum IL-1β, IL-6, nitric oxide (NO) levels, and incidence of adverse reactions were assessed before and after 4 weeks of treatment.Results: The study group had a significantly higher total efficacy/effectiveness (86.11 %) compared to control group (53.89 %). Both groups showed initial decreases and subsequent increases in VAS and PROM from week 1 to week 12 after treatment. However, the study group had significantly lower VAS scores, angle of forward flexion, abduction, and external rotation at all time points compared to control group (p < 0.05). At week 4, the study group had significantly higher FMA-U, ADL, and MBI scores, as well as significantly lower IL-1β, IL-6, and NO levels when compared to control group (p < 0.05).Conclusion: Ultrasound-guided SAB steroid injection improves the efficacy of HSP after CS, improves joint mobility, and reduces serum levels of IL-1β, IL-6, and NO. Further large-sample studies with increased sample sizes and longer investigation periods are needed to validate these findings.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of allogeneic platelet-rich plasma on the healing of ulcer wound surface in streptozotocin-induced diabetic rats 异体富血小板血浆对链脲佐菌素诱导的糖尿病大鼠溃疡创面愈合的影响

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.8

Yueze Chen, Xiaowei Liu, Kesu Hu

{"title":"Effect of allogeneic platelet-rich plasma on the healing of ulcer wound surface in streptozotocin-induced diabetic rats","authors":"Yueze Chen, Xiaowei Liu, Kesu Hu","doi":"10.4314/tjpr.v22i9.8","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.8","url":null,"abstract":"Purpose: To evaluate the effect of allogeneic platelet-rich plasma (PRP) on ulcer wound surface in streptozotocin-induced diabetic rats.Methods: A total of 20 male Sprague-Dawley (SD) rats were procured as skin ulcer as well as diabetes models, and randomly divided into control and PRP groups, respectively. Their wound surfaces were smeared with 0.9 % normal saline or an equal concentration of allogeneic PRP, respectively, and the pathological changes in the wound tissues were examined with the aid of hematoxylin-eosin (H&E) staining. Also, the expression levels of transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in wound tissues were determined using enzyme-linked immunosorbent assay (ELISA).Results: Both purulent exudation and swelling of the wound surface were milder in the PRP group than in the control group, and the PRP group showed greater redness on the wound surface and more pronounced signs of epithelialization of the wound surface and its margins (p < 0.05). Wound healing rate in PRP group was higher than in the control group while the number of fibroblasts and new microvessels in the wound surface in the PRP group were greater than in the control group, accompanied by slighter inflammatory response compared to the control group. Furthermore, the PRP group expressed lower MMP-2, MMP-2/TIMP-2 ratio, and higher TGF-β1 and TIMP-2 levels than in the control group (p < 0.05).Conclusion: Allogeneic PRP treatment contributes to the healing of ulcer wound surface in diabetic rats, a process mediated by TGF-β1/MMP-2/TIMP-2 signaling pathway. Thus, PRP is a potential therapeutic agent for the management of diabetic ulcer wounds.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic effect of Sijunzi decoction on ulcerative colitis: A study based on in vitro functional experiments 四君子汤治疗溃疡性结肠炎的体外功能实验研究

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.12

Guobao Zhang, Wei Li

{"title":"Therapeutic effect of Sijunzi decoction on ulcerative colitis: A study based on in vitro functional experiments","authors":"Guobao Zhang, Wei Li","doi":"10.4314/tjpr.v22i9.12","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.12","url":null,"abstract":"Purpose: To investigate the efficacy of Sijunzi decoction (SJZD) against ulcerative colitis (UC) in vitro, and to unravel the probable mechanism of action.Methods: SEC-6 cells were exposed to lipopolysaccharide (LPS) in order to establish an ulcerative colitis model, and then treated with SJZD. CCK-8 assay was employed to evaluate cell viability, while cell apoptosis was determined by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to assess inflammation factors, viz, TNF-α, IL-1β and IL-6, whereas the expression levels of Bax, Bcl-2 and peroxisome proliferatoractivated receptors (PPARs) were evaluated by Western blot.Results: SJZD doses of 80 and 160 mg/L increased cell viability in LPS-induced SEC-6 cells, while Bcl2 and Bax expressions were regulated, and apoptosis inhibited at these doses (p < 0.05), indicating that SJZD attenuated cell apoptosis. Inflammation was also repressed by SJZD, based on the reduced expressions of TNF-α, IL-1β and IL-6 (p < 0.05). Furthermore, SJZD significantly increased PPARα level, thus enhancing cell viability, inhibiting apoptosis as well as inhibiting inflammation (p < 0.05).Conclusion: SJZD lowers cell damage, and inhibits cell apoptosis and inflammation through the activation of PPARα pathway, thus suggesting that SJZD is a potential therapeutic candidate for the treatment of ulcerative colitis.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Procyanidin A1 acts as an antioxidant stressor in gestational diabetes 原花青素A1在妊娠糖尿病中作为抗氧化应激因子

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.13

Mengni Zhu, Liping Liu

{"title":"Procyanidin A1 acts as an antioxidant stressor in gestational diabetes","authors":"Mengni Zhu, Liping Liu","doi":"10.4314/tjpr.v22i9.13","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.13","url":null,"abstract":"Purpose: To evaluate the mechanism and effect of Procyanidin A1 (PCA1) in gestational diabetes mellitus (GDM).Methods: Human trophoblast cell line HTR-8/Svneo was treated with 25 mM glucose for 24 h, and the effect of PCA1 on HTR-8/SVneo cell viability and proliferation was determined by CCK-8 assay and EdU proliferation kit. The expression of BAX, Bcl-2, cleaved Caspase-3 and cleaved caspase-9 was determined by western blotting. Cell apoptosis was assessed by Annexin V-FITC/ PI staining. Cellular generation of reactive oxygen species (ROS) was determined by ROS assay kit while superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were determined by the corresponding assay kits. The effect of PCA1 on Nrf2/HO-1 pathway was evaluated by determining the expression of Keap1, Nfr2, and HO-1.Results: Procyanidin A1 (PCA1) increased the viability of high glucose (HG) -induced HTR-8/SVneo cells and promoted cell proliferation. Furthermore, PCA1 significantly inhibited HG-induced BAX, cleaved caspase-3, and cleaved caspase-9 expression, resulting in further reduction in HG-induced cell apoptosis. High glucose (HG) induced a significant increase in intracellular ROS levels, and this HGinduced oxidative stress was inhibited by PCA1. Furthermore, PCA1 activated Nrf2/HO-1 pathway and this was responsible for its proliferative, anti-apoptosis and anti-oxidative effects.Conclusion: Procyanidin A1 promotes proliferation, and inhibits apoptosis and oxidative stress induced by high glucose in trophoblast cells by activating Nrf2/HO-1 signaling pathway. Therefore, it is a potential drug for the treatment of GDM.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of activating blood circulation and removing blood stasis on recurrence of alcoholic acute pancreatitis, and the possible underlying mechanism 活血化瘀对酒精性急性胰腺炎复发的影响及可能的机制

4区医学

Tropical Journal of Pharmaceutical Research Pub Date : 2023-10-08 DOI: 10.4314/tjpr.v22i9.11

Haicheng Dong, Weixing Ying, Shifeng Zhu

{"title":"Effect of activating blood circulation and removing blood stasis on recurrence of alcoholic acute pancreatitis, and the possible underlying mechanism","authors":"Haicheng Dong, Weixing Ying, Shifeng Zhu","doi":"10.4314/tjpr.v22i9.11","DOIUrl":"https://doi.org/10.4314/tjpr.v22i9.11","url":null,"abstract":"Purpose: To determine the effect of activating blood circulation and removing blood stasis on the recurrence of alcoholic acute pancreatitis (AAP), and its possible mechanism.Methods: Sixty healthy rats were selected and assigned to control, model and treatment groups (n =20/group). Treatment group was given Taohong Siwu decoction, while control and model groups were given abdominal injection of 0.9 % normal saline. Serum levels of amylase, pancreatic histopathological score, col Ⅰ content, mRNA expression levels of TGF-β 1, TβRII, and Smad3, as well as sPLA2 activity, were determined.Results: Serum amylase, pancreatic histopathological score and col Ⅰ content were significantly reduced in treatment group, relative to model group, while relative mRNA expressions of TGF- β 1, TβRII and Smad3 at each time point were significantly lower than those in the model group (p < 0.05). Serum PLA2 activity and sPLA2 - π a mRNA expression were significantly lower than those in the model group (p < 0.05).Conclusion: Activation of blood circulation and removal of blood stasis significantly prevents the relapse of AAP in rats through regulation of PLA2 activity and TGF-β1/TβRII/Smad3 signaling pathway. Cohort studies in humans would be required to confirm the application of Taohong Siwu decoction to prevent recurrence of AAP.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0