IF 2.6 4区医学

Toxicon Pub Date : 2024-11-16 DOI: 10.1016/j.toxicon.2024.108179

R.M.M.K. Namal Rathnayaka , P.E. Anusha Nishanthi Ranathunga , Y.N.M.P. Abeyrathne , Damsara A. Kularatne , S.A.M. Kularatne

引用次数: 0

Venom characterization of Venezuelan scorpion Tityus caripitensis 委内瑞拉蝎子 Tityus caripitensis 的毒液特征。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-14 DOI: 10.1016/j.toxicon.2024.108174

Amini Hudefe , Aurora Álvarez , Deyanell Hernández , Cecilia Castillo , Caridad Malave , Pedro Parrilla , Noraida Zerpa

{"title":"Venom characterization of Venezuelan scorpion Tityus caripitensis","authors":"Amini Hudefe , Aurora Álvarez , Deyanell Hernández , Cecilia Castillo , Caridad Malave , Pedro Parrilla , Noraida Zerpa","doi":"10.1016/j.toxicon.2024.108174","DOIUrl":"10.1016/j.toxicon.2024.108174","url":null,"abstract":"<div><div><em>Tityus caripitensis</em> is an endemic scorpion species found in the northeastern region from Venezuela, being responsible for sting accidents in this area. This study describes for the first time a biological, biochemical and electrophysiological partial characterization of <em>Tityus caripitensis</em> scorpion venom. The venom is toxic to mice with a LD50 of 20.2 μg/gr mouse. Animals experimentally envenomed with <em>Tityus caripitensis</em> venom gradually manifested clinical signs in response to sublethal doses. SDS-PAGE of the venom resulted in 7 fractions ranging in size from ∼3.5 to ≥38 kDa. The 6–8 kDa proteins could correspond to neurotoxins. In addition, the components of <em>Tityus caripitensis</em> venom were similar to those obtained in the electrophoretic profile of <em>Tityus discrepans.</em> The commercial anti- <em>Tityus discrepans</em> IgG showed reactivity against <em>Tityus caripitensis</em> venom. <em>Tityus caripitensis</em> venom could induce hematological changes such as hyperamylasemia and hyperglycemia. The venom modified voltage dependent Na <sup>+</sup> <sub>v</sub>1.4 channels and blocked K<sub>v</sub> + channels. Although <em>Tityus caripitensis</em> venom is less toxic than <em>Tityus discrepans</em>, they share molecular and antigenic components. This aspect should be considered in the application of antivenom treatment.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108174"},"PeriodicalIF":2.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of the poisonous plants of Lebanon and their effects 黎巴嫩有毒植物及其影响概述。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-13 DOI: 10.1016/j.toxicon.2024.108177

Tharwat El Zahran , Zeina Halabi , Alondra Barakat , Rony Imad El Hachem , Charbel Bou Nicolas , Sally Al Hassan , Aline Khalil

{"title":"An overview of the poisonous plants of Lebanon and their effects","authors":"Tharwat El Zahran , Zeina Halabi , Alondra Barakat , Rony Imad El Hachem , Charbel Bou Nicolas , Sally Al Hassan , Aline Khalil","doi":"10.1016/j.toxicon.2024.108177","DOIUrl":"10.1016/j.toxicon.2024.108177","url":null,"abstract":"<div><div>Poisonous plants are naturally found in the environment and are easily reachable especially by children. These plants pose significant risks ranging from mild or asymptomatic to severe and even life-threatening. Data on poisonous plants of Lebanon is scarce and scattered; therefore, there remains a significant gap in the literature concerning poisonous plants in Lebanon. This study relied on a thorough review of existing literature on poisonous plants of Lebanon and their effects. Based on our experience in the field and on leveraging available data from the literature, a list of important potentially toxic plants in Lebanon was compiled. Toxic plants in Lebanon were categorized based on their chemical properties into groups such as alkaloids; glycosides; proteins, peptides, and lectins; phenols and phenylpropanoids; terpenes and resins; carboxylic acids; and other (uncategorized). The clinical effects of these plants were discussed in detail to provide an overview of the toxicity that they can cause. This study is part of our ongoing work on poisonous plants of Lebanon. It aims to fill a gap pertaining to poisonous plant; it will benefit healthcare workers and the public at the same time. Prompt recognition of plant exposure and their manifestations will allow for better clinical management especially among emergency healthcare workers and professionals. In addition, this review will increase awareness of Lebanese public about the poisonous plants of Lebanon with the ultimate aim to prevent these toxic occurrences from the beginning.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108177"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Naja kaouthia snake venom composition and in-vitro enzymatic activities of 29 specimens in captivity: Highlighting the importance of individual variation in venom pool production 分析人工饲养的 29 条 Naja kaouthia 蛇的毒液成分和体外酶活性：强调毒液池生产中个体差异的重要性。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-13 DOI: 10.1016/j.toxicon.2024.108173

Beatriz Kopel , Caroline Serino-Silva, Rebeca Barcelos Jantsch , Igor Castellar Sorila, Sávio S. Sant’Anna, Kathleen Fernandes Grego, Anita Mitico Tanaka-Azevedo

{"title":"Analysis of Naja kaouthia snake venom composition and in-vitro enzymatic activities of 29 specimens in captivity: Highlighting the importance of individual variation in venom pool production","authors":"Beatriz Kopel , Caroline Serino-Silva, Rebeca Barcelos Jantsch , Igor Castellar Sorila, Sávio S. Sant’Anna, Kathleen Fernandes Grego, Anita Mitico Tanaka-Azevedo","doi":"10.1016/j.toxicon.2024.108173","DOIUrl":"10.1016/j.toxicon.2024.108173","url":null,"abstract":"<div><div><em>Naja kaouthia</em> is a medically important snake, widely distributed throughout Southeast Asia, with a diverse venom composition. <em>N. kaouthia</em> venom is subject to significant intraspecific variation, caused by several factors, such as the wide geographic distribution of the species, sexual and ontogenetic factors. However, individual variation is a factor that has only been studied with small sample size groups and/or with pooled samples. With this in mind, this study evaluates the composition and in-vitro enzymatic activities of 29 individual venom samples from specimens born in captivity, with a similar genetic background caused by inbreeding, using SDS-PAGE under reducing conditions, RP-HPLC profiles and enzymatic activities of PLA<sub>2</sub>, LAAO and proteolytic activity over azocasein. Even in this scenario, we were able to observe significant variations in abundance and activity of PLA<sub>2</sub>. Individual variations in LAAO activity, as well as a sexual dimorphism in which males present a significantly higher LAAO activity than females were observed. Phosphodiasterase and CRiSP abundance were also found and considered to have multiple effects in the clinical manifestations of envenomation by presenting synergistic effects with other proteins from <em>N. kaouthia</em> venom. The RP-HPLC profiles were better at detecting compositional differences than SDS-PAGE profiles and better correlated with enzymatic activities, being a better technique to screen variation profiles and reinforcing the importance of individual venom analysis prior to pooling.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108173"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro anticoagulant effects of Bungarus venoms on human plasma which are effectively neutralized by the PLA2-inhibitor varespladib Bungarus 毒液对人体血浆的体外抗凝作用可被 PLA2 抑制剂 varespladib 有效中和。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-13 DOI: 10.1016/j.toxicon.2024.108178

Abhinandan Chowdhury , Bryan G. Fry , Stephen P. Samuel , Ashish Bhalla , Sakthivel Vaiyapuri , Parul Bhargava , Rebecca W. Carter , Matthew R. Lewin

{"title":"In vitro anticoagulant effects of Bungarus venoms on human plasma which are effectively neutralized by the PLA2-inhibitor varespladib","authors":"Abhinandan Chowdhury , Bryan G. Fry , Stephen P. Samuel , Ashish Bhalla , Sakthivel Vaiyapuri , Parul Bhargava , Rebecca W. Carter , Matthew R. Lewin","doi":"10.1016/j.toxicon.2024.108178","DOIUrl":"10.1016/j.toxicon.2024.108178","url":null,"abstract":"<div><div>Bungarus (krait) envenomings are well-known for their life-threatening neurotoxic effects. However, their impact on coagulation remains largely unexplored experimentally or clinically. This study, examined the effect of begins to examine venoms from four <em>Bungarus</em> species—<em>B. caeruleus, B. candidus, B. fasciatus,</em> and <em>B. flaviceps</em> on human platelet poor plasma coagulation parameters using thromboelastography and coagulation inhibition assays. <em>B. flaviceps</em> completely inhibited clotting, while <em>B. caeruleus</em> only delayed clot formation. In contrast, <em>B. candidus</em> and <em>B. fasciatus</em> did not affect clotting. Subsequent examinations into the anticoagulant biochemical mechanisms demonstrated divergent pathophysiological pathways. <em>B. caeruleus</em> venom anticoagulant effects were prevented by the addition of an excess of phospholipids, with anticoagulation thereby the result of phospholipid depletion. In contrast <em>B. flaviceps</em> anticoagulation was not affected by the addition of an excess of phospholipids. Further investigations demonstrated that <em>B. flaviceps</em> mediates its anticoagulant toxicity through the inactivation of coagulation enzymes. The anticoagulant effects of both <em>B. flaviceps</em> and <em>B. caeruleus</em> were nullified by varespladib, a phospholipase A<sub>2</sub> (PLA<sub>2</sub>) inhibitor, revealing the toxin class involved. These results uncover previously unrecognized and unexplored anticoagulant effects of <em>Bungarus</em> venoms.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108178"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of botulinum toxin A in tissue repair and regeneration 肉毒杆菌毒素 A 在组织修复和再生中的应用。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-13 DOI: 10.1016/j.toxicon.2024.108172

Xuan-Zhu Guo , Ya-Nan Niu , Xuan Zhou , Qiao Wei , Meng Li , Jia-Ning Xia , Yu-Qi Cui , Chao-Xin Chai , Yi-Ming Wang , Li-Ping Chen

引用次数: 0

Corrigendum to “Comparative analysis of protein profiles in skin secretions of some Rana species: Preliminary insights into antimicrobial activity” [Toxicon, 250 (2024) 108110] 对 "一些蕉属物种皮肤分泌物中蛋白质特征的比较分析：抗菌活性的初步见解" [Toxicon, 250 (2024) 108110]。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-12 DOI: 10.1016/j.toxicon.2024.108130

Ebru Tanrıverdi o, Dinçer Ayaz, Yiğit Terzi

引用次数: 0

Study repair function of mucin-2 on the tight junction protein of uterine epithelial cells under bacterial endotoxins 研究细菌内毒素作用下粘蛋白-2对子宫上皮细胞紧密连接蛋白的修复功能

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-08 DOI: 10.1016/j.toxicon.2024.108162

Dujian Yan , Mengru Zhou , Tian Tian , Chenchen Wu

{"title":"Study repair function of mucin-2 on the tight junction protein of uterine epithelial cells under bacterial endotoxins","authors":"Dujian Yan , Mengru Zhou , Tian Tian , Chenchen Wu","doi":"10.1016/j.toxicon.2024.108162","DOIUrl":"10.1016/j.toxicon.2024.108162","url":null,"abstract":"<div><div>To analysis repair function of mucin-2(MUC2) and glycoprotein particles on the tight junction protein of uterus under bacterial endotoxins. In this experiment, we showed that the thicker mucus layer of the uterus is used to prevent the translocation of endotoxin at 21d postdelivery. When endotoxin acts on the uterus to thin its mucous layer, the cells in the lamina propria of the uterus secrete a large number of glycoprotein particles at 27d postdelivery. Due to a significantly decrease in the expression of glycosyltransferase, the glycoprotein particles are incompletely glycosylation MUC2, which can interact with the cell membrane and are released in large quantities in the form of exocytosis. These glycoprotein particles can significantly repair tight junction proteins in the inter-cellular space and significantly increase the expression of Claudin-1, JAM (Junction adhesion molecule-A), E-cadherin, ZO-1(Zonula occludens-1) and desmosome proteins after endotoxin treatment. The results of the present study show that endotoxins can thin the uterine mucus layer and accelerate the release of incompletely glycosylated MUC2 from lamina propria cells. In inter-cellular spaces, MUC2 can increase its expression levels and distribution area to repair the tight junction structure of cells with larger gaps. Further strengthening of the barrier prevents endotoxin translocation by repairing the tight junction structure of uterine epithelial cells.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108162"},"PeriodicalIF":2.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual paralytic shellfish poisoning toxins in Cayuga Lake, New York 纽约卡尤加湖中的异常麻痹性贝类中毒毒素。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-07 DOI: 10.1016/j.toxicon.2024.108165

Zacharias J. Smith , Gregory L. Boyer

引用次数: 0

Bee venom and melittin: Potent key enzyme inhibitors with promising therapeutic potential 蜂毒和美乐汀：具有治疗潜力的强效关键酶抑制剂。

IF 2.6 4区医学

Toxicon Pub Date : 2024-11-05 DOI: 10.1016/j.toxicon.2024.108164

Bayram Alparslan , Murat Şentürk , Cengiz Erkan

{"title":"Bee venom and melittin: Potent key enzyme inhibitors with promising therapeutic potential","authors":"Bayram Alparslan , Murat Şentürk , Cengiz Erkan","doi":"10.1016/j.toxicon.2024.108164","DOIUrl":"10.1016/j.toxicon.2024.108164","url":null,"abstract":"<div><div>Bee venom (BV) is a versatile product with extensive applications, boasting antibacterial and anticancer properties. Within this study, we focused on isolating melittin (Mel) from <em>Apis mellifera</em> L. venom and exploring the influence of both BV and Mel on specific enzymes, namely carbonic anhydrase (CA) I, CA II, CA IX, glutathione reductase (GR), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and neuraminidase (NA). The rationale for selecting these enzymes is that their inhibitors have a particular interest in medicinal chemistry in the treatment of several diseases. BV was obtained using a poison collection apparatus, and Mel was isolated by means of High-Performance Liquid Chromatography (HPLC). All enzymes, except for CA I and CA II, were commercially sourced and of high purity, and the enzyme assays were carried out spectrophotometrically.</div><div>Our findings showed that BV inhibited the enzymes with IC<sub>50</sub> values of 0.583–3.32 ng/mL, and Mel showed an inhibition range of 0.528–3.2 ng/mL. These results underscore the potential therapeutic promise of BV and Mel as robust enzyme inhibitors, offering prospects for addressing diverse health conditions.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"252 ","pages":"Article 108164"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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