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The influence of protein kinase KIN10 gene expression on root phenotype of Arabidopsis thaliana root system in condition of energy stress. 能量胁迫条件下蛋白激酶KIN10基因表达对拟南芥根系表型的影响
TSitologiia i genetika Pub Date : 2016-07-01
E E Krasnoperova, S V Isayenkov, A I Yemets, Ya B Blume
{"title":"The influence of protein kinase KIN10 gene expression on root phenotype of Arabidopsis thaliana root system in condition of energy stress.","authors":"E E Krasnoperova,&nbsp;S V Isayenkov,&nbsp;A I Yemets,&nbsp;Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was shown phenotypic changes in the root system of seedlings Arabidopsis thaliana in transgenic lines with overexpression and suppressed gene expression of serine-threonine protein kinase KIN10 in conditions of energy shortage and under normal conditions. The normal growth and development of KIN10 overexpressing plants with in energy deficiency conditions were detected. The significant inhibition of the plant development under normal conditions for these plant lines was obsereved. The levels of KIN10 gene expression under normal conditions in different organs A. thaliana, particularly in the roots, stems, leaves and flowers were analyzed. The highest level expression of the gene was fixed in the leaves.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential expression of a cysteine proteinase and cystatin pair as side­by­side fusion forms in Escherichia coli. 半胱氨酸蛋白酶和胱抑素对在大肠杆菌中作为并排融合形式的差异表达。
TSitologiia i genetika Pub Date : 2016-07-01
A Gholizadeh
{"title":"Differential expression of a cysteine proteinase and cystatin pair as side­by­side fusion forms in Escherichia coli.","authors":"A Gholizadeh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As a basic study, the fusion expressions of two functionally related proteins were described. The side by side fusion construction, expression, purification and functional characterization of Arabidopsis papain-like cysteine proteinase (CP) and cysteine proteinase inhibitor (CPI) were successfully carried out by using an Escherichia coli expression system without affecting the recombinant bacterial growth. The purification products of two different fused constructs designated as «R1: H2N-maltose binding protein-CPI-CP-COOH and R2: H2N-maltose binding protein-CP-CPI-COOH» showed inverse enzymatic/inhibitory activities, in vitro. Analysis of the constructs by using computational tools revealed that the arrangement of CP/CPI pair in fusion forms might be the important criteria for proper tertiary organization, structural folding and functional property. The overall results showed that the C-terminally located molecule could be the active folded structure in each fusion construct. The achievements of the present work may be utilized in a specific protein engineering application such as manufacturing the novel switchable expression systems in the future.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"16-25"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sr33 & Sr35 gene homolog indentification in genomes of cereals related with Aegilops tauschii & Triticum monococcum. 与大豆和小麦相关的谷物基因组Sr33和Sr35基因同源性鉴定。
TSitologiia i genetika Pub Date : 2016-07-01
B V Ivaschuk, Ya V Pirko, A P Galkin, Ya B Blume
{"title":"Sr33 & Sr35 gene homolog indentification in genomes of cereals related with Aegilops tauschii & Triticum monococcum.","authors":"B V Ivaschuk,&nbsp;Ya V Pirko,&nbsp;A P Galkin,&nbsp;Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using bioinformatics analysis, the homologues of the genes Sr33 and Sr35 were identifed in the genomes of Triticum aestivum, Hordeum vulgare and Triticum urartu. It is known that these genes provide resistance to hightly virulent wheat stem rust races (Ug99). To identify important for resistance amino acid sites, the comparison of the founded homologues with the Sr33 and Sr35 protein sequences was performed. It was found that the sequences S5DMA6 and E9P785 are the closest homologues of RGA1e protein – a product of the Sr33 gene, and the sequences M7YFA9 (CNL-C) and F2E9R2 are the homologues of CNL9 – a product of the gene Sr35. It is assumed that the homologues of the genes Sr33 and Sr35, which derived from the wild relatives of wheat and barley, can provide resistance to various forms of a stem rust and can be used in the future breeding programs for wheat improvement.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"26-37"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The polyphasic taxonomic analysis of the strain Bacillus sp. C6 – phytopathogenic microorganisms antagonist. 芽孢杆菌C6 -植物病原微生物拮抗剂的多相分类分析。
TSitologiia i genetika Pub Date : 2016-07-01
A Yu Grabova, I V Dragovoz, L B Zelena, A N Ostapchuk, L V Avdeeva
{"title":"The polyphasic taxonomic analysis of the strain Bacillus sp. C6 – phytopathogenic microorganisms antagonist.","authors":"A Yu Grabova,&nbsp;I V Dragovoz,&nbsp;L B Zelena,&nbsp;A N Ostapchuk,&nbsp;L V Avdeeva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The polyphasic taxonomic analysis of the strain-antagonist of phytopathogenic bacteria and micromycetes was carried. It was found that the combination of culture-morphological, physiological and biochemical properties allowed to attribute the strain to the group Bacillus subtilis. It was shown that fatty acids of the cell walls of the strain were represented mainly by branched derivatives of iso- and anti iso- C15:0 and C17:0 fatty acids (85%) which was typical for the Bacillus amyloliquefaciens species. After molecular genetic analysis of the nucleotide sequence of the 16S rRNA gene and studying the profile of polymorphic nucleotides the strain was attributed to subspecies Bacillus amyloliquefaciens subsp. plantarum.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"62-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibiting effect of agmatine on genetically programmed death of leukocytes in experimental diabetes mellitus. 胍丁胺对实验性糖尿病白细胞遗传程序性死亡的抑制作用。
TSitologiia i genetika Pub Date : 2016-07-01
I V Ferents, I V Brodyak, M Ya Lyuta, V A Burda, N O Sybirna
{"title":"Inhibiting effect of agmatine on genetically programmed death of leukocytes in experimental diabetes mellitus.","authors":"I V Ferents,&nbsp;I V Brodyak,&nbsp;M Ya Lyuta,&nbsp;V A Burda,&nbsp;N O Sybirna","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study is an attempt to elucidate of agmatine effects upon leukocyte apoptosis in experimental diabetes mellitus (EDM). We demonstrated the increase in numbers of the leukocytes with both early and late signs of apoptosis at diabetes. Further changes in the morphofunctional state of the leukocytes include the increased amount of fragmented DNA, elevated apoptotic index and violated ratio of p53 to Bcl-2 proteins. Agmatine has been shown to exert direct corrective effects on leukocyte apoptosis: the content of р53 and Bcl-2 proteins was normalized, apoptotic index was decreased, the process of nuclear DNA degradation was ceased, while the amount of cells with early and late signs of apoptosis was diminished.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"50-61"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A case of prenatal detection of a de novo unbalanced complex chromosomal rearrangement involving four chromosomes. 一例产前检测的新生不平衡复杂染色体重排涉及四条染色体。
TSitologiia i genetika Pub Date : 2016-07-01
L Y Pylyp, D O Mykytenko, L O Spinenko, K V Lavrova, N V Verhoglyad, V D Zukin
{"title":"A case of prenatal detection of a de novo unbalanced complex chromosomal rearrangement involving four chromosomes.","authors":"L Y Pylyp,&nbsp;D O Mykytenko,&nbsp;L O Spinenko,&nbsp;K V Lavrova,&nbsp;N V Verhoglyad,&nbsp;V D Zukin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Complex chromosomal rearrangements are rarely observed prenatally. Genetic counceling of CCR carriers is complicated, especially in cases of de novo origin of the rearrangement. Here we present a new case of a de novo CCR involving four chromosomes observed in amniotic fluid cells of the fetus at 17 weeks of gestation. The rearrangement was characterized as an apparently balanced four-way translocation t(1;11;7;13)(~p21;~q13.5;~q32;~q22)dn by conventional cytogenetic studies. However, array-based comparative genomic hybridization revealed 5 submicroscopic heterozygous interstitial deletions on chromosome 1, 11, 7, 13 with a total loss of 21.1 Mb of genetic material in regions close to those, designated as breakpoints by conventional cytogenetic analysis. The described case clearly illustrates that high-resolution molecular genetic analysis should be combined with conventional cytogenetic techniques to exclude subtle chromosomal abnormalities in CCR cases detected prenatally.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 4","pages":"74-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophila melanogaster. 利用黑腹果蝇修复基因mus209的LacZ报告活性进行诱变试验。
TSitologiia i genetika Pub Date : 2016-05-01
Y Yasinskiy, L V Omelyanchuk, O V Zhuk, I A Kozeretska
{"title":"Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophila melanogaster.","authors":"Y Yasinskiy,&nbsp;L V Omelyanchuk,&nbsp;O V Zhuk,&nbsp;I A Kozeretska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We studied a set of Drosophila melanogaster strains that could be potentially suitable for testing a variety of mutagenic factors. Their genomes contained insertions of the enhancer trap P{lacW} in which the activity of the LacZ reporter is under the control of the reparation genes’ regulatory region. We demonstrated that the beta-galactosidase reporter, which is encoded by insertion of P{lacW} element in the gene mus209, is induced by irradiation in the cells of the salivary glands and wing imaginal discs. Despite the fact that the reporting coloration is not associated with the dose of radiation treatment, we found that the induction threshold of the reporter is different for these tissues. Thus, coloration in salivary glands is detectable after the dose of 200 rad and above, whereas the imaginal discs get colored with 500 rad and above. Thereby, multiple thresholds for induction of the reporter in the various tissues allow approximating the received dose.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"12-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic marking of glume colour in Triticum spelta L. var. caeruleum using gliadins. 麦胶蛋白对小麦颖片颜色的遗传标记。
TSitologiia i genetika Pub Date : 2016-05-01
N A Kozub, I A Sozinov, A K Niniyeva, Ye V Tverdokhleb, Ya B Blume, R L Boguslavskii
{"title":"Genetic marking of glume colour in Triticum spelta L. var. caeruleum using gliadins.","authors":"N A Kozub,&nbsp;I A Sozinov,&nbsp;A K Niniyeva,&nbsp;Ye V Tverdokhleb,&nbsp;Ya B Blume,&nbsp;R L Boguslavskii","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic control of dark color of glumes was studied in Triticum spelta L. var. caeruleum accessions using gliadins as genetic markers. F2 and BC1 plants from crosses between the spelt accessions and common wheat varieties with white glumes served as the material for the investigation. Analysis of segregation for glume colour fitted the monogenic control of the character. Using electrophoretical analysis of gliadin of seeds from the hybrid plants it was revealed that in the T. spelta var. caeruleum accessions the allele Gli-A1j* is linked to the allele for dark (black) glumes at the Rg-A1 locus.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"24-9"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of Tlr2, Defa, Muc2 genes in rat duodenal upon long-term gastric hypacidity and with multiprobiotic correction. 长期胃酸过低及多种益生菌校正后大鼠十二指肠Tlr2、Defa、Muc2基因的表达
TSitologiia i genetika Pub Date : 2016-05-01
A S Dranitsina, K O Dvorshchenko, O O Morgaienko, L L Ostapchenko
{"title":"Expression of Tlr2, Defa, Muc2 genes in rat duodenal upon long-term gastric hypacidity and with multiprobiotic correction.","authors":"A S Dranitsina,&nbsp;K O Dvorshchenko,&nbsp;O O Morgaienko,&nbsp;L L Ostapchenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Expression of Tlr2, Muc2, Defa genes in rat duodenal upon long-term gastric hypacidity (hypochlorhydria) and with multiprobiotic correction was investigated. Increasing of Tlr2, Muc2, Defa expression on the background of the intensification of lipid peroxidation in rat duodenal villus and crypt epithelial cells upon gastric hypoacidity was shown. The mRNAs patterns of genes mentioned above were shown to decrease or approach to control values as well as the level of lipid peroxidation products under the treatment of hypoacidic rats with the multiprobiotic. The data obtained may indicate involvement of genes Tlr2, Muc2, Defa in development of inflammation in the duodenum due to dysbiotic changes under conditions of prolonged hypochlorhydria.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"57-65"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variation of microsatellite loci in Vincetoxicum Wolf. species in the south-east of Ukraine. 灰狼微卫星位点的变异。乌克兰东南部的一种。
TSitologiia i genetika Pub Date : 2016-05-01
A E Demkovych, A Z Glukhov, S N Privalikhin, V M Ostapko, I V Makogon, Ya V Pirko, Ya B Blume
{"title":"Variation of microsatellite loci in Vincetoxicum Wolf. species in the south-east of Ukraine.","authors":"A E Demkovych,&nbsp;A Z Glukhov,&nbsp;S N Privalikhin,&nbsp;V M Ostapko,&nbsp;I V Makogon,&nbsp;Ya V Pirko,&nbsp;Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The genetic diversity of 13 Vincetoxicum Wolf species distributed in Ukraine was investigated using 4 of the 8 nuclear microsatellite markers, previously developed for Vincetoxicum atratum from Japan. The number of alleles ranged from 8 to 25. The expected heterozygosities were 0,690–0,938 and observed ones ranged from 0,205 to 0,806. In general, the level of genetic variation in studied representatives of the genus Vincetoxicum from Ukraine proved to be comparable with that of Vincetoxicum atratum. The microsatellite loci Vinc5, Vinc104, Vinc123, Vinc124 can be successfully used to assess intra- and interspecific polymorphisms of species of the genus Vincetoxicum Wolf in Ukraine.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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