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Cell genetic engineering – transmission genetics of plants. 细胞基因工程--植物的传递遗传学。
TSitologiia i genetika Pub Date : 2017-03-01
N Kuchuk
{"title":"Cell genetic engineering – transmission genetics of plants.","authors":"N Kuchuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been described achievements of cell and genetic engineering that led to formation of new genetics chapter – transmission genetics. It has been analyzed results and showed new opportunities in the field of transgenomic somatic hybrids and cybrid obtaining, production of transgenic plants with agronomic pharmaceutical application, development of transplastomic plants, accu-mulation of recombinant proteins by using the transient expression of foreign genes in plants.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"40-6"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36724086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. 乌克兰冬季普通小麦品种在抗病基因储存蛋白位点和分子标记方面的多样性。
TSitologiia i genetika Pub Date : 2017-03-01
N A Kozub, I A Sozinov, A V Karelov, Ya B Blume, A A Sozinov
{"title":"Diversity of ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes.","authors":"N A Kozub,&nbsp;I A Sozinov,&nbsp;A V Karelov,&nbsp;Ya B Blume,&nbsp;A A Sozinov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Diversity of Ukrainian winter common wheat varieties was studied with respect to the storage protein loci Gli-A1, Gli-B1, Gli-D1, Glu-A1, Glu-B1, Glu-D1, Gli-A3, Gli-B5, and Gli-A6 (362 varieties) and markers for the Lr34/Yr18/Pm38/Sr57/Bdv1 gene conferring moderate resistance to a number of biotrophic pathogens, the Tsn1 gene for sensitivity to the toxins A of the necrotrophic fungi Pyrenophora triticirepentis and Stagonospora nodorum, the Tsc2 gene for sensitivity to the toxin B of P. triticirepentis, and the TDF_076_2D gene for moderate resistance to Fusarium head blight (181 varieties). Significant differences in frequencies of alleles at these marker loci between groups of varieties developed in different soil and climatic zones were revealed. The retention of a set of predominant alleles of a certain zone in different periods of breeding was confirmed. At the same time, the appearance of new allele associations in the groups of varieties of the Steppe (in particular Gli-A1g and Glu-B1al) and the Central Forest-Steppe (1AL/1RS and Glu-B1d) in the last two decades has been noted. Nonrandom associations between alleles of disease resistance genes as well as alleles of disease resistance genes and storage protein alleles were revealed</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"59-73"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36773350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolating and confirming the Mudr­inserted flanking sequences of maize. 玉米mudr插入侧翼序列的分离与鉴定。
TSitologiia i genetika Pub Date : 2017-03-01
W F Yang, Y H Tian, T T Wang, R N Wang, Y S Tao
{"title":"Isolating and confirming the Mudr­inserted flanking sequences of maize.","authors":"W F Yang,&nbsp;Y H Tian,&nbsp;T T Wang,&nbsp;R N Wang,&nbsp;Y S Tao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>MuDR exhibits the highest transposition activity and insertional mutagenesis frequency in Mutator (Mu) family. If we isolate the MuDR­insertion­specific flanking sequences (MuDRFs), it will be crucial for using Mu element­mediated mutants. The MuDR­TAIL­PCR system was constructed and optimized using a combination of MuDR­TIR­nested specific primers and 12 arbitrary degenerate (AD) primers, modified reaction system and procedure and mutant DNA templates of 87 genotypes from M2 or M2:3 families created by crossing the W22::Mu line (active MuDR donor parent) from the UniformMu population with the Zong31 (Z31) line (recipient parent). Here 129 different MuDRFs were acquired by MuDR­TAIL­PCR, accounting for 86.60 % of the total mutant­specific agarose gel bands. In addition, we confirmed the authenticity of the non­redundant flanking sequence amplifications. The amplified non­redundant flanking sequences accounted for 65.12 % of the total MuDRFs, and 88.00 % of the non­redundant MuDRFs were inserted inside the genes. These results show that the MuDR­TAIL­PCR system that we developed can be used for specifically isolating MuDRFs.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"89-90"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36724089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
125 years of virology and ascentof biotechnologies based on viral expression. 125 年病毒学和基于病毒表达的生物技术的兴起。
TSitologiia i genetika Pub Date : 2017-03-01
A Giritch, V Klimyuk, Y Gleba
{"title":"125 years of virology and ascentof biotechnologies based on viral expression.","authors":"A Giritch, V Klimyuk, Y Gleba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study of viruses lasts for more than a century since their discovery in 1892. In recent decades, viruses are also being actively exploited as a biotechnological tool. Plant-virus-driven transient expression of heterologous proteins is an actively developing production platform; it is the basis of several industrial processes that are currently being used for the production of multiple recombinant proteins. Viral vectors have also become useful tools for research. Viral vectors delivered by Agrobacterium (magnifection) provide for high pro-tein yield, rapid scale up and fast manufacturing. In this review, we explore modern approaches for bio technological production of recombinant proteins in plants using viral vectors.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"19-39"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36773346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plants and microgravity: patterns of microgravity effects at the cellular and molecular levels. 植物与微重力:微重力效应在细胞和分子水平上的模式。
TSitologiia i genetika Pub Date : 2017-03-01
E L Kordium, D K Chapman
{"title":"Plants and microgravity: patterns of microgravity effects at the cellular and molecular levels.","authors":"E L Kordium,&nbsp;D K Chapman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An overview on the effects of real and simulated microgravity on certain cell components and processes, including new information obtained recently, is presented. Attention is focused on the influence of microgravity on the cytoplasmic membrane state, transcriptome and proteome, cell wall remodeling, and Ca2+-signaling in plant cells that are not specialised to gravity perception. It is emphasized the exceptional significance of the data on the organ-specific remodeling of the transcriptome and proteome in response to space flight, that discovers new advanced approaches to implement the fundamental and applied problems of plant space biology.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"47-58"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36773347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of salt resistance of arabidopsis salicylatedeficient transformants NahG by influence of nitric oxide donor. 一氧化氮供体对拟南芥水杨酸盐缺乏转化体NahG耐盐性的影响
TSitologiia i genetika Pub Date : 2017-03-01
T O Yastreb, Yu V Karpets, Yu E Kolupaev, A P Dmitriev
{"title":"Induction of salt resistance of arabidopsis salicylatedeficient transformants NahG by influence of nitric oxide donor.","authors":"T O Yastreb,&nbsp;Yu V Karpets,&nbsp;Yu E Kolupaev,&nbsp;A P Dmitriev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of treatment with the donor of nitric oxide sodium nitroprusside (SNP – 0,5 mM) on the salt resistance of Arabidopsis plants of wild-type (Col-0) and transformant with the gene of bacterial salicylatehydroxylase (NahG) was compared. The basic resistance against the salt stress (200 mM of NaCl) in transformants NahG was higher. In the conditions of salt stress these plants differed in the higher activity of antioxidant enzymes and the raised content of carbohydrates and anthocyans. The treatment with NO donor increased the salt resistance of plants of both genotypes that expressed in the decrease of growth inhibition, reduction of oxidative damages and preservation of chlorophyll pool in the leaves. After the influence of salt stress the activity of superoxide dismutase and guaiacol peroxidase in treated with SNP wild type plants and transformants was higher, than in the appropriate untreated plants. In the wild type plants, treated with NO donor, the content of proline in leaves after the salt stress was lower, than in untreated. The treatment with SNP of transformants NahG, on the contrary, led to more essential increase of proline content in leaves under the salt stress. The conclusion is made that plants of wild type Col-0 and transformant NahG have differences in the functioning of protective systems under the salt stress, and the induction of protective systems in A. thaliana plants under the influence of nitric oxide can occur without salicylate participation.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36724088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular characterization of Fusarium resistance from Elymus repens introgressed into bread wheat. 面包小麦渐渗黑麦抗镰刀菌的分子特性研究。
TSitologiia i genetika Pub Date : 2017-03-01
G Fedak, W Cao, D Wolfe, D Chi, A Xue
{"title":"Molecular characterization of Fusarium resistance from Elymus repens introgressed into bread wheat.","authors":"G Fedak,&nbsp;W Cao,&nbsp;D Wolfe,&nbsp;D Chi,&nbsp;A Xue","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A cross was made of Elymus repens onto the wheat cultivar Crocus and BC1 progeny advanced to BC1F7 by single seed descent. Sixteen lines were selected based on agronomic performance and evaluated in an FHB epiphytotic nursery. Eight lines with FHB resistance were selected. Based on GISH analysis, line P1142-3-1-5 had 42 chromosomes with one pair of chromosomes showing telomeric translocations on both arms. This chromosome was identified as 3D by using SSR markers. An evaluation of lines with single translocations revealed that FHB resistance was contributed by the translocation on the long arm of chromosome 3D. That line has minimal linkage drag and should be amenable to applications in breeding for disease resistance.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"74-8"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36724090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Double helix of the journal «Сytology and genetics»: fity years later. 《Сytology与遗传学》杂志的双螺旋结构:五十年后。
TSitologiia i genetika Pub Date : 2017-03-01
Ya B Blume
{"title":"Double helix of the journal «Сytology and genetics»: fity years later.","authors":"Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This survey paper contains a brief analysis of publications included in current issue of scientific journal «Cytology and Genetics» dedicated to its 50th anniversary. These papers reflect scientific achievements of their authors in the field of genetics and cell biology and underine the potential of these two biological disciplines, forming «double helix» of the journal.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 2","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36773348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphogenesis in vitro in inbreds of maize Lancaster heterotic group. 玉米兰开斯特杂种群近交系离体形态发生研究。
TSitologiia i genetika Pub Date : 2017-01-01
K V Derkach, O E Abraimova, T M Satarova
{"title":"Morphogenesis in vitro in inbreds of maize Lancaster heterotic group.","authors":"K V Derkach,&nbsp;O E Abraimova,&nbsp;T M Satarova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The genotypic features of morphogenesis and regeneration in vitro for five maize inbreds of perspective breeding Lancaster heterotic group compared to representatives of others heterotic groups – PLS61, A188 and Chi31 were studied. It was identified that the ratio of such types of morphogenesis as organogenesis and embryoidogenesis in callus culture was determined by the explant genotype and the concentration of sucrose in the medium for callusogenesis. The frequency of embryoidogenesis as the most effective type of morphogenesis for further regeneration in Lancaster inbreds averaged about 40.0 ± 12.8 %, while for other heterotic groups it was only 14.0 ± 4.0 %. For Lancaster heterotic group sucrose at the concentration of 30 g/l in the medium for callusogenesis provided further regeneration through embryoidogenesis at the level of 26.5 ± 15.4 %, but sucrose at the concentration of 60 g/l provided it at 57.7 ± 19.8 %. For inbreds which represent other heterotic groups sucrose content in the medium for callusogenesis did not affect further regenaration, the level of embryoidogenesis at 30 and 60 g/l sucrose amounted 11.0 ± 7.0 and 15.0 ± 4.8 % correspondingly.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36773344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel Alui-polymorphism in the fourth intron of the chicken growth hormone gene. 鸡生长激素基因第4内含子的alui多态性。
TSitologiia i genetika Pub Date : 2017-01-01
R A Kulibaba, Y V Liashenko, P S Yurko
{"title":"A novel Alui-polymorphism in the fourth intron of the chicken growth hormone gene.","authors":"R A Kulibaba,&nbsp;Y V Liashenko,&nbsp;P S Yurko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A novel AluI-polymorphism in the fourth intron of chicken growth hormone gene was shown. It was detected the cytosine to thymine transition in the restriction site for AluI. Primers, that flanking the 460 bp fragment of the fourth intron, containing a polymorphic restriction site for AluI, was designed. The nucleotide sequence fragments amplified polymorphic variants was determined. Using designed primers was analyzed the genetic structure of populations of White Plymouth Rock, Poltava Clay, Rhode Island Red and Borkovskaya Barvistaya chicken breeds. It was found that growth hormone gene (by AluI-polymorphism in the fourthintron) was polymorphic in all experimental populations. Frequencies of alleles C and T in chicken population of White Plymouth Rock breed were 0,14 and 0,86; Rhode Island Red – 0,3 and 0,7; Poltava Clay – 0,04 and 0,96; Borkovskaya Barvistaya – 0,08 and 0,92 respectively. The tendency to increase egg production and egg weight of chicken with C/C genotype, as well as meat quality (live weight, carcass weight, weight of pectoral muscles) of chickens with genotype T/T of Rhode Island Red chicken breed was shown.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"51 1","pages":"76-7"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36713529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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