Induction of salt resistance of arabidopsis salicylatedeficient transformants NahG by influence of nitric oxide donor.

Abstract

The influence of treatment with the donor of nitric oxide sodium nitroprusside (SNP – 0,5 mM) on the salt resistance of Arabidopsis plants of wild-type (Col-0) and transformant with the gene of bacterial salicylatehydroxylase (NahG) was compared. The basic resistance against the salt stress (200 mM of NaCl) in transformants NahG was higher. In the conditions of salt stress these plants differed in the higher activity of antioxidant enzymes and the raised content of carbohydrates and anthocyans. The treatment with NO donor increased the salt resistance of plants of both genotypes that expressed in the decrease of growth inhibition, reduction of oxidative damages and preservation of chlorophyll pool in the leaves. After the influence of salt stress the activity of superoxide dismutase and guaiacol peroxidase in treated with SNP wild type plants and transformants was higher, than in the appropriate untreated plants. In the wild type plants, treated with NO donor, the content of proline in leaves after the salt stress was lower, than in untreated. The treatment with SNP of transformants NahG, on the contrary, led to more essential increase of proline content in leaves under the salt stress. The conclusion is made that plants of wild type Col-0 and transformant NahG have differences in the functioning of protective systems under the salt stress, and the induction of protective systems in A. thaliana plants under the influence of nitric oxide can occur without salicylate participation.