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A mutagenicity and cytotoxicity study of limonium effusum aqueous extracts by Allium, Ames and MTT tests. 用葱属植物、艾姆斯和MTT试验研究积液柠檬水提物的致突变性和细胞毒性。
TSitologiia i genetika Pub Date : 2015-03-01
Y Eren, A Ozata, M Konuk, D Akyil, R Liman
{"title":"A mutagenicity and cytotoxicity study of limonium effusum aqueous extracts by Allium, Ames and MTT tests.","authors":"Y Eren,&nbsp;A Ozata,&nbsp;M Konuk,&nbsp;D Akyil,&nbsp;R Liman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nowadays plants or plant extracts have become very important for alternative medicine. Plants and their extracts have many therapeutical advantages but some of them are potentially toxic, mutagenic, carcinogenic and teratogenic. Root, stem and leafparts of Limonium effusum were used in this study and this species is an endemic species for Turkey. Mutagenic and cytotoxic effects of root, stem and leaf aqueous extracts were observed with Allium, Ames and MTT tests. Allium root growth inhibition test and mitotic index studies showed that aqueous extracts have dose-dependent toxic effects. Chromosome aberration studies indicated that especially sticky chromosome, anaphase-telophase disorder and laggard chromosome anomalies were highly observed. Ames test performed with Limonium effusum root aqueous extracts, showed weak mutagenic effects in Salmonella typhimurium TA98 strain with S9. MTT test based on mitochondrial activity indicated that most of the aqueous extracts have cytotoxic effects. This study aimed to determine the possible mutagenic and cytotoxic effects of L. effusum aqueous extracts by using bacterial, plant and mammalian cells. This research showed that some low concentrations of the L. effusum extracts have inhibited cytotoxic effects but high concentrations have induced cytotoxicity. On the other hand only a weak mutagenic activity was identified by Ames test with TA98 S9(+).</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"56-65"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33351233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin N-dependent proteolytic pathway. d -氨基酸及其代谢物可能参与拟南芥半胱氨酸蛋白酶/胱抑素n依赖性蛋白水解途径。
TSitologiia i genetika Pub Date : 2015-03-01
A Gholizadeh
{"title":"The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin N-dependent proteolytic pathway.","authors":"A Gholizadeh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cysteine proteinases and their inhibitors 'cystatins' play essential roles in plant growth and development. They are involved in various signaling pathways and in the response to wide ranges of biotic and abiotic environmental stresses. To investigate their possible influence from D-amino acids or their metabolism in vivo, Arabidopsis seedlings were allowed to grow under four physicochemically different D-amino acids including D-aspartate, D-serine, D-alanine and D-phenylalanine containing media. The reverse transcription polymerase chain reaction (R T-PCR) analysis of cysteine proteinase and cystatin gene expressions showed that the addition of D-amino acid to the plant growth media considerably induce the expression of proteinase transcript while decrease the expression level of inhibitor gene in the leaf and root tissues of the test plant in overall. Based on the obtained results the potential impact of D-amino acids or their metabolism on the activity of cysteine proteinase/cystatin-dependent proteolytic apparatus as well as their possible cooperation were predicted and discussed in the plant system.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system. 水凝胶纳米颗粒包封质粒是一种合适的基因传递系统。
TSitologiia i genetika Pub Date : 2015-03-01
A Rafieei, F Riazi-Rad, M H Alimohammadian, T Gazori, S M R Fatemi, M Havaskary
{"title":"Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system.","authors":"A Rafieei,&nbsp;F Riazi-Rad,&nbsp;M H Alimohammadian,&nbsp;T Gazori,&nbsp;S M R Fatemi,&nbsp;M Havaskary","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmidcomplex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be Alg/Chi nanoparticles.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ITPA gene variant may protect against anemia induced during pegylated interferon alfa and ribavirin combination treatment in Ukrainian patients with chronic hepatitis C. ITPA基因变异可能保护乌克兰慢性丙型肝炎患者在聚乙二醇化干扰素和利巴韦林联合治疗期间引起的贫血。
TSitologiia i genetika Pub Date : 2015-03-01
A Kucherenko, V Pampukha, Bobrova I, L Moroz, L Livshits
{"title":"ITPA gene variant may protect against anemia induced during pegylated interferon alfa and ribavirin combination treatment in Ukrainian patients with chronic hepatitis C.","authors":"A Kucherenko,&nbsp;V Pampukha,&nbsp;Bobrova I,&nbsp;L Moroz,&nbsp;L Livshits","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was to clarify the association between the inosine triphosphate pyrophosphatase (ITPA) gene variants and PEG-IFNalpha/RBV combination treatment induced anemia in chronic hepatitis C (CHC) Ukrainian patients. The data were collected from 80 CHC patients with HCV genotype 1 infection. All study participants received standard doses of PEG-IFNalpha and RBV According to the Hb level changes patients were distributed into: case group--42 patients with combination treatment induced anemia, and control group--38 patients with no signs of anemia. Genotyping for ITPA gene rs1127354 and rs7270101 variants was performed using PCR followed by RFLP assay. Fisher's exact test was used to estimate the difference in genotype and allelic distribution. Distribution of rs 7270101 genotypes was not significantly different between groups of CHC patients with RB Vinduced anemia and without it. The frequency of rs1127354 A allele carriers was significantly higher (P < < 0,05) in group of CHC patients without anemia (23.7%) comparing to the group ofpatients with anemia (7.3%). The respective allele frequency in control group (13.2%) was almost 3-fold higher (P < 0,05) comparing to the case group (4.9%). Significant association of ITPA gene rs1127354 with protection against RB V-induced hemolytic anemia was found in Ukrainian patients with CHC infection. Rs1127354 variant may assist as a pharmacogenetic marker in HCV antiviral therapy correction for side effect avoidance..</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"38-41"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The mechanisms of Antartic vascular plants adaptation to abiotic environmental factors]. [南极维管植物对非生物环境因子的适应机制]。
TSitologiia i genetika Pub Date : 2015-03-01
I P Ozheredova, I Yu Parnikoza, O O Poronnik, I A Kozeretska, S V Demidov, V A Kunakh
{"title":"[The mechanisms of Antartic vascular plants adaptation to abiotic environmental factors].","authors":"I P Ozheredova,&nbsp;I Yu Parnikoza,&nbsp;O O Poronnik,&nbsp;I A Kozeretska,&nbsp;S V Demidov,&nbsp;V A Kunakh","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"72-9"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33351237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Influence of tissue-specific superoxide dismutase genes expression in brain cells on Drosophila melanogaster sensitivity to oxidative stress and viability]. [脑组织组织特异性超氧化物歧化酶基因表达对黑腹果蝇氧化应激敏感性和生存能力的影响]。
TSitologiia i genetika Pub Date : 2015-03-01
M V Vitushynska, N P Matiytsiv, Y Chernyk
{"title":"[Influence of tissue-specific superoxide dismutase genes expression in brain cells on Drosophila melanogaster sensitivity to oxidative stress and viability].","authors":"M V Vitushynska,&nbsp;N P Matiytsiv,&nbsp;Y Chernyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study has shown that both functional gene knockout Sodl and Sod2 and their overexpression in neurons and glial tissue increase the sensitivity of Drosophila melanogaster to oxidative stress (OS) conditions. The lowest survival rate was only 20.5% in insects with Sod2 knockout in neurons. Comparative analysis of the survival curves showed that adults with altered tissue-specific expression of the studied genes had reduced average and maximum life span. Under OS conditions induced by 5% hydrogen peroxide the life spans of wild type Oregon R and transgenic insects were significantly reduced. Altered Sod gene expression in glial tissue leads to degenerative changes in Drosophila brain at the young age. During the aging of insects and the action of pro-oxidants increasing of neurodegenerative phenotype is observed.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"21-8"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Allelic variants of the gene bamyl barley in Eastern European and central Asian areas]. [东欧和中亚地区大麦基因的等位基因变异]。
TSitologiia i genetika Pub Date : 2015-03-01
O R Stratula, R N Kalendar, Yu M Sivolap
{"title":"[Allelic variants of the gene bamyl barley in Eastern European and central Asian areas].","authors":"O R Stratula,&nbsp;R N Kalendar,&nbsp;Yu M Sivolap","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The collections of varieties of spring barley cultivars from the Eastern European and Central Asian areas were analyzed by exonpecific PCR (EPIC) for beta-amylase genes. The endosperm beta-amylase gene (bamyl) was differentiated by the presence of 126 bp MITE insertion into intron 3 that is associated with low activity beta-amylase. The findings suggest that a low level of genetic variation for bamylgene within climatic zones is associated with individual breeding program for each climatic zone.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"11-5"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Role of transcription factors in transdifferentiation of the gastric mucosa]. 转录因子在胃粘膜转分化中的作用。
TSitologiia i genetika Pub Date : 2015-03-01
S V Vernygorodskyi, L V Degtiariova, O I Iatsyna, Ya B Blume, A I Yemets
{"title":"[Role of transcription factors in transdifferentiation of the gastric mucosa].","authors":"S V Vernygorodskyi,&nbsp;L V Degtiariova,&nbsp;O I Iatsyna,&nbsp;Ya B Blume,&nbsp;A I Yemets","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The analysis of intestinal differentiation transcription factor CDX2 in the gastric mucosa biopsies has been carried out. It was established that CDX2 by itself promoter activation pathway can obtain intestinal phenotype for gastric mucosa cells. The loss of CDX2 expression in the nuclei of metaplastic epithelium may serve as a predictor of gastric mucosa malignization.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"42-7"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33351238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Genetic diversity and mating system Pinus brutia var. Stankewiczii sukacz. in small localities of Sudak (Crimea)]. [j] .野松(Pinus brutia var. Stankewiczii sukacz)的遗传多样性和交配系统。在苏达克(克里米亚)的小地方]。
TSitologiia i genetika Pub Date : 2015-03-01
I I Korshikov, L A Kalafat, Ya G Milchevskaya
{"title":"[Genetic diversity and mating system Pinus brutia var. Stankewiczii sukacz. in small localities of Sudak (Crimea)].","authors":"I I Korshikov,&nbsp;L A Kalafat,&nbsp;Ya G Milchevskaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A comparative analysis of genetic variation at 12 polymorphic isozyme loci, and the mating system has been carried out in mature trees and their seed progeny in three small localities of Pinus brutia var. stankewiczii Sukacz. near the town of Sudak--settlement of Novyi Svet in the Crimea. We found that embryos maintain the same allelic diversity as mother plants but their observed heterozygosity is lower on the average by 37.4%. The significant deviation of genotype distribution from the theoretically expected ratios caused by the deficiency of heterozygotes was observed at 8 out of 12 loci. Multilocus estimate of outcrossing rate (t(m)) in populations varied from 68.9 to 94.9% making on the average 80.7%.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33349295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cytogenetic genome stabilization in secalotriticum (x Triticosecale derzhavinii secalotriticum rozenst., et Mittelst., s/RRAABB, 2n = 42)]. [j] .黑麦的细胞遗传学基因组稳定性[j]。， et Mittelst。[s/RRAABB, 2n = 42]。
TSitologiia i genetika Pub Date : 2015-03-01
O M Lyusikov, L A Gordei
{"title":"[Cytogenetic genome stabilization in secalotriticum (x Triticosecale derzhavinii secalotriticum rozenst., et Mittelst., s/RRAABB, 2n = 42)].","authors":"O M Lyusikov,&nbsp;L A Gordei","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The article presents the results of cytogenetic research of the genome stabilization in hexaploid secalotriticum F(1-9) (s/RRAABB, 2n = 6x = 42). Nucleo-cytoplasmic specificity and cytogenetic factors of genome stabilization were established in secalotriticum: subspecies specificity of cytotype, genotype specificity of hybrids, origin, polar centromere orientation, division and segregation type of the chromosomes in meiosis. The necessity for developing independent trends in heteroplasmic triticales breeding was substantiated.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 2","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33351236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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