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[GENE POOL SIMILARITIES AND DIFFERENCES BETWEEN UKRAINIANS AND RUSSIANS OF SLOBOZHANSHINA ON Y-CHROMOSOME DATA]. [乌克兰人和俄罗斯人在y染色体数据上的基因库异同]。
TSitologiia i genetika Pub Date : 2015-07-01
O M Utevska, A S Pshenichnov, Kh D Dibirova, S Rootsi, A T Agdzhoyan, M I Churnosov, E V Balanovska, L A Atramentova, O P Balanovsky
{"title":"[GENE POOL SIMILARITIES AND DIFFERENCES BETWEEN UKRAINIANS AND RUSSIANS OF SLOBOZHANSHINA ON Y-CHROMOSOME DATA].","authors":"O M Utevska,&nbsp;A S Pshenichnov,&nbsp;Kh D Dibirova,&nbsp;S Rootsi,&nbsp;A T Agdzhoyan,&nbsp;M I Churnosov,&nbsp;E V Balanovska,&nbsp;L A Atramentova,&nbsp;O P Balanovsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of the study of Y-chromosomal polymorphisms of Russian and Ukrainian population are presented for Slobozhanshina--contemporary border region, former \"Wild Field\" boundary, which was inhabited in XVII-XVIII centuries by both the Russians from the north and Ukrainians from the west. In general, Ukrainian and Russian populations of Slobozhanshchina genetically are very close, their set and frequency range of Y-chromosome haplogroups are typical for the Eastern Europe. But a detailed analysis of highly informative Y-chromosome markers showed that after 3,5 centuries of coexistence on the same historical territory, the both nations retain the ethnic specificity of their gene pools: Ukrainian populations are similar to the rest of Ukraine, and Russian populations are similar to the south of the European part of Russia. The genetic differences may be due to the spatial characteristics of marriage migration and the predominant ethnic environment.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 4","pages":"40-50"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34216980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[PREIMPLANTATION DEVELOPMENT OF HUMAN EMBRYOS WITH NUMERICAL CHROMOSOME ABNORMALITIES IN VITRO]. [体外数字染色体异常人类胚胎的着床前发育]。
TSitologiia i genetika Pub Date : 2015-07-01
O V Chaplia, J V Gontar, N M Bilko
{"title":"[PREIMPLANTATION DEVELOPMENT OF HUMAN EMBRYOS WITH NUMERICAL CHROMOSOME ABNORMALITIES IN VITRO].","authors":"O V Chaplia,&nbsp;J V Gontar,&nbsp;N M Bilko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study was focused on morphokynetic characteristics of in vitro cultured human embryos that were considered to be aneuploid or euploid according to the preimplantation genetic screening results. Among all the embryos examined only 34.2% were chromosomally balanced, while others possessed isolated or combined chromosome abnormalities. Although morphological features of cleaving pathologic and euploid embryos did not differ significantly, on the fifth day of culture chromosomally balanced specimen formed \"expanded\" blastocyst twice as frequently as abnormal ones. Moreover, development of 38.4% of aneuploid embryos was compromised before the initiation of cavitation. Thus, prolonged embryo culture advances selection of samples with the highest implantation potential for the transfer on the basis of the morphokynetic characteristics and helps to avoid additional genetic testing.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 4","pages":"51-60"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34216983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[BIOINFORMATICS COMPARISON OF HUMAN AND PLANT PHOSPHATOMES]. [人类和植物磷体的生物信息学比较]。
TSitologiia i genetika Pub Date : 2015-07-01
D A Samofalova, P A Karpov, Ya B Blume
{"title":"[BIOINFORMATICS COMPARISON OF HUMAN AND PLANT PHOSPHATOMES].","authors":"D A Samofalova,&nbsp;P A Karpov,&nbsp;Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study presents the results of bioinformatic comparison of protein phosphatases from higher plants and human phosphatom (150 sequences). Based on sequence and profile comparison with known catalytic domains, 204 plant homologues from Physcomitrella patens and Arabidopsis thaliana where selected. Clustering of joint group of plant and mammalian protein phosphatases revealed fundamental differences in plant and human phosphatomes. At the same time, it was shown significant differences in the set of protein phosphatases in P. patens, A. thaliana, and such monocots as Orysa saliva and Zea mays.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 4","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34215563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MULTIPLEX PCR ASSAY FOR DETECTION OF HUMAN SOMATOTROPIN AND INTERFERON ALPHA2b GENES IN PLANT MATERIAL. 用于检测植物材料中的人类 SOMATOTROPIN 和 INTERFERON ALPHA2b 基因的多重 PCR 分析。
TSitologiia i genetika Pub Date : 2015-05-01
I M Gerasymenko, M G Mazur, Y V Sheludko, N V Kuchuk
{"title":"MULTIPLEX PCR ASSAY FOR DETECTION OF HUMAN SOMATOTROPIN AND INTERFERON ALPHA2b GENES IN PLANT MATERIAL.","authors":"I M Gerasymenko, M G Mazur, Y V Sheludko, N V Kuchuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using transgenic plants as factories for production of physiologically active human proteins arouses special concern because occasional escape of such transgenes into environment may cause health problems. Creation of plant varieties producing pharmaceutically valuable proteins should be accompanied by development of detection methods suitable for controlling the transgene behavior. Here we describe a multiplex PCR protocol for revealing of two human genes (encoding growth hormone and interferon alpha2b) that have been successfully introduced into plant genomes. The primer pair designed for detection of human growth hormone coding sequence amplifies fragments of different size from the full-length gene in the human genome and the intronless coding sequence usually used for plant transformation. Application of this primer pair may be recommended for ruling out false positive results due to sample contamination with human DNA. Such a control may be useful also in PCR analysis during establishing of transgenic plants carrying genes of human origin.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[THE ROLE OF STREPTOMYCES NOGALATER Lv65 snoaM, snoaL and snoaE GENES IN NOGALAMYCIN BIOSYNTHESIS]. 诺加霉素链霉菌Lv65、snoaL和snoaE基因在诺加霉素生物合成中的作用。
TSitologiia i genetika Pub Date : 2015-05-01
D Klymyshin, O Stephanyshyn, V Fedorenko
{"title":"[THE ROLE OF STREPTOMYCES NOGALATER Lv65 snoaM, snoaL and snoaE GENES IN NOGALAMYCIN BIOSYNTHESIS].","authors":"D Klymyshin,&nbsp;O Stephanyshyn,&nbsp;V Fedorenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of phylogenetic analysis indicate high similarity of SnoaM, SnoaL SnoaE to the cyclases involved in the biosynthesis of various antibiotics. Genes snoaM, snoaL and snoaE disruption in S. nogalater chromosome was carried on and S. nogalater MI, LI and EI strains were generated. The gene replacement events in M1, L1 and E1 were verified by Southern hybridization. Recombinant strains were characterised by lack of nogalamycin biosynthesis. Originally, M1, L1 and E1 were complemented with plasmids expressing putative cyclase genes from S. nogalater leading to restoration of nogalamycine production. The complementation results clearly indicate that obtained strains are cyclase deficient mutants. Furthermore, complementation of M1, L1 and E1 with a cyclase genes from wild-type strain is consistent with the suggested function of these enzymes.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[MORPHOFUNCTIONAL PARAMETERS OF BLOOD CELLS OF THE RAT WITH 1,2-DIMETHYLHYDRAZINE-INDUCED COLON CARCINOGENESIS]. 1,2-二甲基肼致结肠癌大鼠血细胞形态功能参数。
TSitologiia i genetika Pub Date : 2015-05-01
I V Byelinska, O V Lynchak, T V Rybalchenko, S V Yablonska, O M Bahurynska, V K Rybalchenko
{"title":"[MORPHOFUNCTIONAL PARAMETERS OF BLOOD CELLS OF THE RAT WITH 1,2-DIMETHYLHYDRAZINE-INDUCED COLON CARCINOGENESIS].","authors":"I V Byelinska,&nbsp;O V Lynchak,&nbsp;T V Rybalchenko,&nbsp;S V Yablonska,&nbsp;O M Bahurynska,&nbsp;V K Rybalchenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphofunctional parameters of blood cells of the rats with dimethylhydrazine induced colon carcinogenesis have been investigated. The reduction of Mean corpuscular hemoglobin (MCH) and Mean corpuscular hemoglobin concentration (MCHC) whereas increasing of reticulocytes number and indirect bilirubin after 20 weeks of experiment of dimethylhydrazine (DMH)-induced colon carcinogenesis indicate the hemolysis of red blood cells due to the DMH influence during tumors development. The moderate increasing of monocytes and platelets number, as well as increment of eosinophilic granulocytes number have been observed. 26 weeks of experiment (after 6 weeks discontinuation of DMH) leads to reduction of hemoglobin and erythrocytes number, a moderate increase of monocytes number and increment of platelets number in rats blood. The anemia with thrombocytosis accompany cancer in humans and the model of DMH-induced colon cancer is appropriate not only for studies of carcinogenesis and searching of new antineoplastic drugs, but also for the development of the correctional approaches of cancer-associated changes in the hematopoietic system.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[CHROMOSOMAL ABNORMALITIES IN PATIENTS WITH INFERTILITY]. 【不孕症患者染色体异常】。
TSitologiia i genetika Pub Date : 2015-05-01
L Y Pylyp, L O Spinenko, N V Verhoglyad, O O Kashevarova, V D Zukin
{"title":"[CHROMOSOMAL ABNORMALITIES IN PATIENTS WITH INFERTILITY].","authors":"L Y Pylyp,&nbsp;L O Spinenko,&nbsp;N V Verhoglyad,&nbsp;O O Kashevarova,&nbsp;V D Zukin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To assess the frequency and structure of chromosomal abnormalities in patients with infertility, a retrospective analysis of cytogenetic studies of 3414 patients (1741 females and 1673 males), referred to the Clinic of reproductive medicine \"Nadiya\" from 2007 to 2012, was performed. Chromosomal abnormalities were detected in 2.37% patients: 2.79% in males and 1.95% in females. Balanced structural chromosomal abnormalities prevailed over numerical abnormalities and corresponded to 80.2% of all chromosomal abnormalities detected in the studied group. Sex chromosome abnormalities made up 23.5% of chromosomal pathology (19/81) and included gonosomal aneuploidies in 84% of cases (16/19) and structural abnormalities of chromosome Y in 16% of cases (3/19). The low level sex chromosome mosaicism was detected with the frequency of 0.55%. Our results highlight the importance of cytogenetic studies in patients seeking infertility treatment by assisted reproductive technologies, since an abnormal finding not only provide a firm diagnosis to couples with infertility, but also influences significantly the approach to infertility treatment in such patients.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"33-9"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[MICROSATELLITE ANALYSIS OF HOMOEOLOGOUS CHROMOSOME GROUP 5 OF INTROGRESSIVE WHEAT LINES TRITICUM AESTIVUM/AMBLYOPYRUM MUTICUM]. [小麦渐渗系第5组同源染色体微卫星分析]。
TSitologiia i genetika Pub Date : 2015-05-01
T S Iefimenko, Yu G Fedak, M Z Antonyuk, T K Ternovska
{"title":"[MICROSATELLITE ANALYSIS OF HOMOEOLOGOUS CHROMOSOME GROUP 5 OF INTROGRESSIVE WHEAT LINES TRITICUM AESTIVUM/AMBLYOPYRUM MUTICUM].","authors":"T S Iefimenko,&nbsp;Yu G Fedak,&nbsp;M Z Antonyuk,&nbsp;T K Ternovska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Introgressive hybridization of common wheat is still one of the most effective way of wheat genome enrichment with resistance genes to stresses. In this context, T genome of Amblyopyrum muticum is a promising one for such wheat improvement. Genome substitution amphidiploid Aurotica (AABBTT) with A and B subgenomes from common wheat cultivar Aurora and with T subgenome from Amblyopyrum muticum is characterized by high level of cold resistance. In wheat, this characteristic associated with chromosomes of the homoeologous group 5. Comparative microsatellite analysis of Aurora and Aurotica identified 9 SSR loci specific to D genome which produced other amplicons with Aurotica DNA spectrum. Primers for these loci were used for screening (Aurora x Aurotica)F5 progeny (234 plants) in purpose to find those individuals, which carry 5T chromosome or its fragments in their genomes. In this study were detected 24 plants with disomic substitutions 5D/5T. All of them were offspring of five F4 plants, which in turn derived from two F2 individuals. A significant number of plants contain recombinant chromosome 5D/5T. The recombination event could occur in any generation since F2.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IS THE AMPLIFICATION OF c-MYC, MLL AND RUNX1 GENES IN AML AND MDS PATIENTS WITH TRISOMY 8, 11 AND 21 A FACTOR FOR A CLONAL EVOLUTION IN THEIR KARYOTYPE? 8、11和21三体AML和MDS患者中c-MYC、MLL和RUNX1基因的扩增是其核型克隆进化的一个因素吗?
TSitologiia i genetika Pub Date : 2015-05-01
S Angelova, B Spassov, V Nikolova, I Christov, N Tzvetkov, M Simeonova
{"title":"IS THE AMPLIFICATION OF c-MYC, MLL AND RUNX1 GENES IN AML AND MDS PATIENTS WITH TRISOMY 8, 11 AND 21 A FACTOR FOR A CLONAL EVOLUTION IN THEIR KARYOTYPE?","authors":"S Angelova,&nbsp;B Spassov,&nbsp;V Nikolova,&nbsp;I Christov,&nbsp;N Tzvetkov,&nbsp;M Simeonova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of our study was 1) to define if the amplification of c-MYC, MLL and RUNX1 genes is related to the progressive changes of the karyotype in patients with AML and MDS with trisomy 8, 11 and 21 (+8, +11 and +21) in bone marrow and 2) can that amplification be accepted as part of the clonal evolution (CE). Karyotype analysis was performed in 179 patients with AML or MDS with the different chromosomal aberrations (CA) aged 16-81. The findings were distributed as follow: initiating balanced CA (n = 60), aneuploidia (n = 55), unbalanced CA (n = 64). Amplification of c-MYC, MLL and RUNX1 genes by means of fluorescence in situ hybridization (FISH) was found in 35% (7 out of 20) of AML and MDS patients with +8, +11 u +21 as single CA in their karyotype; in 63.6% of pts (7 out of 11)--with additional numerical or structural CA and in 75% (9 out of 12)--with complex karyotype. We assume that the amplification of the respective chromosomal regions in patients with +8, +11 and +21 is related to CE. Considering the amplification as a factor of CE, we established 3 patterns of karyotype development depending on the type of the initiating CA in it. Significant statistical differences were found between the three patterns regarding the karyotype distribution in the different stages of progression (p < 0.001).</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[IDENTIFICATION OF MUTATION IVS1-5(G > C) OF THE β-HEMOGLOBIN GENE (Hbβ) BY RDBH-METHOD IN PATIENTS WITH β-THALASSEMIA IN AZERBAIJAN]. 用rdbh法鉴定阿塞拜疆β-地中海贫血患者β-血红蛋白基因(Hbβ)突变IVS1-5(G > C)。
TSitologiia i genetika Pub Date : 2015-05-01
G Akbarova
{"title":"[IDENTIFICATION OF MUTATION IVS1-5(G > C) OF THE β-HEMOGLOBIN GENE (Hbβ) BY RDBH-METHOD IN PATIENTS WITH β-THALASSEMIA IN AZERBAIJAN].","authors":"G Akbarova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The hematological and molecular-genetics analyses of patients with suspected β-thalassemia were done by the RDBH StripAssay. The complete blood evaluation (HB, MCH, MCV, MCHC, RBC, Hct, HbA2, HbF), monitoring of serum iron and ferritin, molecular analysis--RDBH (Reverse Dot-Blot Hybridization StripAssay) were done. Two persons were carriers of the beta-thalassemic trait as β+ thalassemia minor IVS1-5(G > > C)/wt. Mutation IVS1-5 (G > C) in the compound with a mutation IVS1-110 (G > A) or mutation IVS1-6 (T > C) determines the development of β-thalassemia intermedia. RDBH-method is easy and economical method in molecular diagnosis of β-thalassemia, if hematological parameters are incorrect.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"49 3","pages":"40-4"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33871887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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