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Influence of the PCR artifacts on the genotyping efficiency by the microsatellite loci using native polyacrylamide gel electrophoresis. PCR伪影对天然聚丙烯酰胺凝胶电泳微卫星位点分型效率的影响。
TSitologiia i genetika Pub Date : 2016-05-01
R A Kulibaba, Y V Liashenko
{"title":"Influence of the PCR artifacts on the genotyping efficiency by the microsatellite loci using native polyacrylamide gel electrophoresis.","authors":"R A Kulibaba,&nbsp;Y V Liashenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The formation of different types of the artifacts in the amplification reaction with using different classes of the molecular-genetic markers (Indel and SSR) was studied. It was shown that DNA heteroduplexes formed during amplification of heterozygous samples, as fragments of target genes and microsatellite loci. During the electrophoresis in native polyacrylamide gel of the amplification products of homozygous samples for microsatellite loci it was revealed specific additional fragments that do not belong to the class of heteroduplex DNA. It was supposed, that the additional fragments belong to a special type of homoduplex DNA – non-linear homoduplexes. The analysis revealed that the formation of a non-linear homoduplex DNA takes place on the 20–25 cycle of the PCR, both at the amplification of the individual samples, and individual DNA fragments that was cut out from the gel.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"16-23"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bcl1 polymorphism of glucocorticoid receptor gene in patients with bronchial asthma with obesity. 支气管哮喘合并肥胖患者糖皮质激素受体基因Bcl1多态性研究
TSitologiia i genetika Pub Date : 2016-05-01
V V Kmyta, V Y Garbuzova, E N Prystupa, L N Prystupa
{"title":"Bcl1 polymorphism of glucocorticoid receptor gene in patients with bronchial asthma with obesity.","authors":"V V Kmyta,&nbsp;V Y Garbuzova,&nbsp;E N Prystupa,&nbsp;L N Prystupa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objective of this investigation was to analyze possible association between BclI polymorphism of glucocorticoid receptor gene and obesity in patients with bronchial asthma (BA). The study involved 188 patients with bronchial asthma and 95 apparently healthy adult individuals. Generally accepted assessments and examinations for BA diagnosis, and anthropometric, molecular-genetic and statistic methods of investigation were used in the research. It was found out that the patients with BA demonstrated higher body mass index (BMI) and higher ratio of fat centralization much more often, than the control group. Genotypes distribution for BclI polymorphism in patients with BA showed a statistically significant difference between patients with different BMI unlike the control group. Comparison of genotype frequency for BclI polymorphism in glucocorticoid receptor gene in individuals with different ratio of fat centralization in the control group and in the patients with BA separately showed statistically significant differences in the distribution of gene allelic variations only among the patients with BA. It was demonstrated that G/G genotype in the patients with visceral obesity was associated with BA.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reduction of radiation­induced nitrative stress in leucocytes and kidney cells of rats upon administration of polyphenolic complex concentrates from red wine. 红酒多酚复合物浓缩液降低大鼠白细胞和肾细胞辐射诱导的硝化应激。
TSitologiia i genetika Pub Date : 2016-05-01
M Sabadashka, N Sybirna
{"title":"Reduction of radiation­induced nitrative stress in leucocytes and kidney cells of rats upon administration of polyphenolic complex concentrates from red wine.","authors":"M Sabadashka,&nbsp;N Sybirna","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The research has shown that exposure to ionizing radiation at the dose of 30 cGy leads to the activation of NO-synthase way of nitrogen oxide synthesis, as well as to the accumulation of its stable metabolites and 3’-nitrotyrosine modified proteins in rat peripheral blood leucocytes and the renal cortical layer. NO-synthase activity was preserved at the control value through the consumption of red wine natural polyphenolic complex concentrates by the irradiated animals. The content of proteins modified by tyrosine nitration decreased in the early period of post-radiation exposure due to the influence of the investigated concentrate. Thus the ability of red wine natural polyphenolic complex concentrates to prevent adverse changes in L-arginine/NO system and, therefore, inhibit the development of nitrative stress induced by low doses of ionizing radiation has been proved experimentally.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"46-56"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of CYP21A2 gene mutations in patients from Ukraine with congenital adrenal hyperplasia. 乌克兰先天性肾上腺增生患者CYP21A2基因突变分析。
TSitologiia i genetika Pub Date : 2016-05-01
S Yu Chernushyn, L A Livshits
{"title":"Analysis of CYP21A2 gene mutations in patients from Ukraine with congenital adrenal hyperplasia.","authors":"S Yu Chernushyn,&nbsp;L A Livshits","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the article the data on the distribution of CYP21A2 gene mutations (gene deletion/conversion, c.290-13C>A/G, E110Vfs, I172N, cluster of mutations I236N, V237E, M239K, V281L, Q318X, R356W) among Ukrainian patients with congenital adrenal hyperplasia of different clinical phenotypes are presented. The most common mutation in the studied group (n = 27) is the CYP21A2 gene deletion/conversion. Possible patterns of the studied mutations distribution in different populations of the world and the patients’ genotype – phenotype association are discussed.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"42-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Allelic polymorphism C-590T of the gene IL-4 as the most possible genetic marker of high predisposition to development of recurrent episodes of acute obstructive bronchitis in children. IL-4基因C-590T等位基因多态性是儿童急性阻塞性支气管炎复发易感性的最可能的遗传标记。
TSitologiia i genetika Pub Date : 2016-05-01
O Kens, N Vishtak, H Acopyan, V Bergtravm
{"title":"Allelic polymorphism C-590T of the gene IL-4 as the most possible genetic marker of high predisposition to development of recurrent episodes of acute obstructive bronchitis in children.","authors":"O Kens,&nbsp;N Vishtak,&nbsp;H Acopyan,&nbsp;V Bergtravm","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Molecular-genetic study of C-590T polymorphic locus of IL-4 gene in 31 children with recurrent episodes of acute obstructive bronchitis and 50 people who constituted the control population-based group has been carried out. It is proved that the presence of this polymorphic locus leads to three-fold increase of the risk of acute obstructive bronchitis recurrent episodes.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"30-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36769797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of substances with cryoprotective properties on surface marker CD44 in human erythrocytes. 低温保护物质对人红细胞表面标志物CD44的影响。
TSitologiia i genetika Pub Date : 2016-05-01
N G Zemlianskykh
{"title":"Effect of substances with cryoprotective properties on surface marker CD44 in human erythrocytes.","authors":"N G Zemlianskykh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study there were investigated the changes in surface marker CD44 in human erythrocytes exposed to cryoprotective media, as well as the impact of oxidative modification of membrane-cytoskeleton proteins on the CD44 characteristics under the changed physico-chemical parameters of the cellular environment. Glycerol, DMSO, sucrose, and PEG-1500 were shown to cause a decrease in CD44 expression level and amount of CD44-positive cells during prolonged exposure erythrocytes with them. That may reflect subtle adjustments in the system of protein-protein interactions in erythrocyte membrane-cytoskeleton complex, which may affect the stability of cells during cryopreservation. Extracellular substances (sucrose and PEG-1500) exhibited a more pronounced effect on the CD44 in erythrocytes in comparison with the examined substances of endocellular type. Modification of membrane-cytoskeleton proteins with oxidizing bifunctional reagent diamide enhanced the identified tendencies.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 3","pages":"66-79"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36720753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[CYTOSKELETON ORIENTATION IN THE EPIDERMAL CELLS OF ROOTS FORMED DE NOVO ON LEAF EXPLANTS UNDER CLINOROTATION]. [叶片外植体在植物化作用下重新形成的根表皮细胞的细胞骨架取向]。
TSitologiia i genetika Pub Date : 2016-03-01
I V Bulavin
{"title":"[CYTOSKELETON ORIENTATION IN THE EPIDERMAL CELLS OF ROOTS FORMED DE NOVO ON LEAF EXPLANTS UNDER CLINOROTATION].","authors":"I V Bulavin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Root anatomy, cytoskeleton orientation and cell wall thickness in cells of the roots formed de novo in vitro under clinorotation (simulated microgravity) were investigated. Structure of the embryonic roots and of the roots formed de novo in cambium cells of the leaf petiole explants was shown to be similar. Root cell differentiation in vitro under clinorotation did not differ from that in control. Changes of tubulin microtubules' orientation in the epidermis of the distal elongation zone were observed under clinorotation that seems to be associated with specific physiological properties of the cells. Under clinorotation, the tendency of cell wall thinning was detected in the root cells formed in vitro.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"58-64"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF "STRICT RESPONSE" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND MORPHOLOGICAL DIFFERENTIATION IN ACTINOMYCETES]. ppGpp分子在细菌“严格反应”的形成、抗生素的生物合成和放线菌的形态分化中的作用。
TSitologiia i genetika Pub Date : 2016-03-01
D Klymyshin, O Stephanyshyn, V Fedorenko
{"title":"[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF \"STRICT RESPONSE\" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND MORPHOLOGICAL DIFFERENTIATION IN ACTINOMYCETES].","authors":"D Klymyshin,&nbsp;O Stephanyshyn,&nbsp;V Fedorenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Strict response is a pleiotropic physiological response of cells caused by lack of aminoacetylated tRNAs. Experimentally, this response occurs due to the lack of amino acids in the environment and the limitation of tRNA aminoacylation even in the presence of the corresponding amino acids in the cell. Many features of this response indicate its dependence on the accumulation of ppGpp molecules. There is a correlation between the growth rate of actinomycetes and biosynthesis of their secondary metabolites. Introduction of additional relA gene copies of ppGpp synthetase can affect the production of antibiotics in streptomycetes. The article presents the authors' own experimental data, dedicated to the influence of heterologous relA gene expression in Streptomyces nogalater cells.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"65-74"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34563601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A HIGH FREQUENCY OF HERITABLE CHANGES IN NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER IN UKRAINE. 乌克兰黑腹果蝇自然种群的高频率遗传变化。
TSitologiia i genetika Pub Date : 2016-03-01
I A Kozeretska, S V Serga, I Kunda-Pron, O V Protsenko, S V Demydov
{"title":"A HIGH FREQUENCY OF HERITABLE CHANGES IN NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER IN UKRAINE.","authors":"I A Kozeretska,&nbsp;S V Serga,&nbsp;I Kunda-Pron,&nbsp;O V Protsenko,&nbsp;S V Demydov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Spontaneous mutations are thought to have a stable rate for a given species. If non-adaptive, they appear at low frequencies and are governed by drift. However, environmental factors have been reported to cause spread of non-adaptive mutations in populations, governed by mechanisms, such as genetic assimilation. In the present study, we report a simultaneous appearance of a mutant and apparently non-adaptive C2 vein in Drosophila melanogaster at higher than expected frequencies in several distant populations, which excludes the role of drift or selection as the cause of the reported mutation frequencies. We discuss explanations of the phenomenon, including the role of externalfactors, such as temperature, in the possible genetic assimilation of the trait.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"32-5"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[GENETIC VARIABILITY OF MATERNAL PLANTS AND SEED EMBRYOS OF KOCH PINE POPULATIONS (PINUS KOCHIANA KLOTZSCH EX KOCH) IN CRIMEA]. [克里米亚科氏松种群(pinus kochiana klotzsch ex Koch)母系植物和种子胚胎的遗传变异]。
TSitologiia i genetika Pub Date : 2016-03-01
I I Korshykov, L O Kalafat, O M Vynogradova, D Y Podgornyi
{"title":"[GENETIC VARIABILITY OF MATERNAL PLANTS AND SEED EMBRYOS OF KOCH PINE POPULATIONS (PINUS KOCHIANA KLOTZSCH EX KOCH) IN CRIMEA].","authors":"I I Korshykov,&nbsp;L O Kalafat,&nbsp;O M Vynogradova,&nbsp;D Y Podgornyi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Comparative studies of genetic variability were undertaken for 12 allozyme loci selections of trees and embryos of seed, and also for the crossing systems in five populations of Koch pine of (Pinus kochiana Klotzsch ex Koch) in Crimea. It was shown that in seed embryos the allelic variety peculiar to the maternal plants was restored, however the level of the available (H₀) heterozygosity was considerably lower, 0.286 and 0.189 respectively. For the embryos unlike the trees, in the majority of the analyzed loci the considerable divergence was specific in the actual distribution of genotypes from the theoretically expected according to Hardy- Weinberg law. The proportion of cross pollination at the unilocal (t(s)) estimation varied from 0.384 to 0.673 in the populations, while at the multilocal ones (t(m)) it was 0.639-0.841.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"36-43"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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