TSitologiia i genetika最新文献_第7页

CONSTRUCTION AND APPLICATIONS OF A MYCORRHIZAL ARBUSCULAR SPECIFIC cDNA LIBRARY. 菌根丛枝特异性cDNA文库的构建与应用。

TSitologiia i genetika Pub Date : 2016-03-01

S Isayenkov, F J M Maathuis

{"title":"CONSTRUCTION AND APPLICATIONS OF A MYCORRHIZAL ARBUSCULAR SPECIFIC cDNA LIBRARY.","authors":"S Isayenkov, F J M Maathuis","doi":"","DOIUrl":"","url":null,"abstract":"To exploit the potential benefits of mycorrhizas, we need to investigate the processes that occur in these symbiotic interactions, particularly in the arbuscular compartment where nutrients are exchanged between the plant and the fungus. Progress in this area is restricted due to the intricacy and complexity of this plant-fungus interface and many techniques that have been employed successfully in other plants and animal systems cannot be used. An effective approach to study processes in arbuscules is to examine transcript composition and dynamics. We applied laser capture microdissection (LCM) to isolate approximately 3000 arbuscules from Glomus intraradices colonised Me- dicago truncatula roots. Total RNA was extracted from microdissected arbuscules and subjected to T7 RNA polymerase-based linear amplification. Amplified RNA was then usedfor construction of a cDNA library. The presence and level of enrichment of mycorrhiza-specific transcripts was determined by quantitative Real-time and conventional PCR. To improve enrichment a cDNA library subtraction was performed. Complementation of yeast mutants deficient in the uptake of.potassium, phosphate, sulphate, amino acids, ammonium and of a Mn²⁺sensitive strain, demonstrates the functionality of our cDNA library.","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"3-12"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34626647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Elie Metchnikoff: father of phagocytosis theory and pioneer of experiments in vivo]. [Elie Metchnikoff:吞噬理论之父和体内实验先驱]。

TSitologiia i genetika Pub Date : 2016-03-01

V Korzh, P Bregestovski

引用次数: 0

IDENTIFICATION OF QTLs ON CHROMOSOME 1B FOR GRAIN QUALITY TRAITS IN BREAD WHEAT (TRITICUM AESTIVUM L.). 面包小麦籽粒品质性状1B染色体qtl的鉴定

TSitologiia i genetika Pub Date : 2016-03-01

M Shahzad, S H Khan, A S Khan, M Sajjad, A Rehman, A I Khan

引用次数: 0

THE HOMOLOGOUS IDENTIFICATION OF THE STEM RUST RESISTANCE GENES RDg5, Adf3 AND RGA1 IN THE RELATIVES OF BARLEY. 大麦茎秆抗锈病基因RDg5、Adf3和RGA1的同源性鉴定。

TSitologiia i genetika Pub Date : 2016-03-01

B V Ivaschuk, D O Samofalova, Ya V Pirko, G Fedak, Ya B Blume

{"title":"THE HOMOLOGOUS IDENTIFICATION OF THE STEM RUST RESISTANCE GENES RDg5, Adf3 AND RGA1 IN THE RELATIVES OF BARLEY.","authors":"B V Ivaschuk, D O Samofalova, Ya V Pirko, G Fedak, Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"The barley genes Rpg5, RGA1 and Adf3, which provide a strong resistance to many pathotypes of stem rust, were cloned a few years ago, but it was still unclear whether their homologues were represented in wheat and in related species. The paper describes the results of a bioinformatic research to determine the homologues of Rpg5, RGA1 and Adf3 in the genomes of Triticum aestivum and several wild grasses, which breeders usually use as sources of stem rust resistance, and which are available in the genome databases. It was found that the Th. elongatum sequence Q9FEC6 and T. aestivum sequence Q43655 were the high identical homologues of the Adf3 sequence. T. urartu M8A999 sequence and T. aestivum W5FCU1 sequence were found to be the closest homologues of Rpg5 complete protein sequence, but the identity of their kinase domains were not as clear as that of the other domains. The separate Rpg5 kinase part analysis did not provide the strong evidences that its orthologs were presented in our corn species. T urartu M7ZZX9 sequence and T. aestivum W5FFP0 and W5F133 sequences were showed to be the homologues of RGA1. The analysis of the predicted active sites allowed finding out the difference between sequences of Rpg5, RGA1, Adf3 protein and their homologues.","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"21-31"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Ya.B. BLUME (on the occasion of the 60th anniversary of the birth)]. [Ya.B。[在诞辰60周年之际]。

TSitologiia i genetika Pub Date : 2016-03-01

引用次数: 0

[INHERITANCE OF EPIDERMIS PIGMENTATION IN SUNFLOWER ACHENES]. 向日葵瘦果表皮色素沉着的遗传研究。

TSitologiia i genetika Pub Date : 2016-03-01

N A Gorohivets, E V Vedmedeva

引用次数: 0

INDUCED CYTOMICTIC VARIATIONS AND SYNCYTE FORMATION DURING MICROSPOROGENESIS IN PHASEOLUS VULGARIS L. 小孢子发生过程中诱导的巨细胞变异和合胞形成。

TSitologiia i genetika Pub Date : 2016-03-01

G Kumar, N Chaudhary

{"title":"INDUCED CYTOMICTIC VARIATIONS AND SYNCYTE FORMATION DURING MICROSPOROGENESIS IN PHASEOLUS VULGARIS L.","authors":"G Kumar, N Chaudhary","doi":"","DOIUrl":"","url":null,"abstract":"The intercellular translocation of chromatin material along with other cytoplasmic contents among the proximate meiocytes lying in close contact with each other commonly referred as cytomixis was reported during microsporogenesis in Phaseolus vulgaris L., a member of the family Fabaceae. The phenomenon of cytomixis was observed at three administered doses of gamma rays viz. 100, 200, 300 Gy respectively in the diploid plants of Phaseolus vulgaris L. The gamma rays irradiated plants showed the characteristic feature of inter-meiocyte chromatin/chromosomes transmigration through various means.such as channel formation, beak formation or by direct adhesion between the PMC's (Pollen mother cells). The present study also reports the first instance of syncyte formation induced via cytomictic transmigration in Phaseolus vulgaris L. Though the frequency of syncyteformation was rather low yet these could play a significant role in plant evolution. It is speculated that syncyte enhances the ploidy level of plants by forming 2n gametes and may lead to the production ofpolyploid plants. The phenomenon of cytomixis shows a gradual inclination along with the increasing treatment doses of gamma rays. The preponderance of cytomixis was more frequent during meiosis I as compared to meiosis II. An interesting feature noticed during the present study was the channel formation among the microspores and fusion among the tetrads due to cell wall dissolution. The impact of this phenomenon is also visible on the development of post-meiotic products. The formation of heterosized pollen grains; a deviation from the normal pollen grains has also been reported. The production of gametes with unbalanced chromosomes is of utmost importance and should be given more attention in future studies as they possess the capability of inducing variations at the genomic level and can be further utilized in the improvement of germplasm.","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 2","pages":"50-7"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GENETIC DIVERSITY OF SOME IRANIAN SWEET CHERRY (PRUNUS AVIUM) CULTIVARS USING MICROSATELLITE MARKERS AND MORPHOLOGICAL TRAITS. 利用微卫星标记和形态性状分析伊朗甜樱桃品种的遗传多样性。

TSitologiia i genetika Pub Date : 2016-01-01

A Farsad, M Esna-Ashari

{"title":"GENETIC DIVERSITY OF SOME IRANIAN SWEET CHERRY (PRUNUS AVIUM) CULTIVARS USING MICROSATELLITE MARKERS AND MORPHOLOGICAL TRAITS.","authors":"A Farsad, M Esna-Ashari","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to characterize 23 important Iranian sweet cherry (Prunus avium) cultivars collected from different provinces of Iran and 1 foreign cultivar, which was used as control, considered for breeding programs by using 21 microsatellite markers and 27 morphological traits. In sweet cherry (Prunus avium) accessions, leaf, fruit, and stone morphological characters were evaluated during two consecutive years. The study revealed a high variability in the set of evaluated sweet cherry accessions. The majority of important correlations were determined among variables representing fruit and leaf size and variables related to color. Cluster analysis distinguished sweet cherry accessions into two distinct groups. Principal component analysis (PCA) of qualitative and quantitative morphological parameters explained over 86.59% of total variability in the first seven axes. In PCA, leaf traits such as leaf length and width, and fruit traits such as length, width, and weight, and fruit flesh and juice color were predominant in the first two components, indicating that they were useful for the assessment of sweet cherry germplasm characterization. Out of 21 SSR markers, 16 were polymorphic, producing 177 alleles that varied from 4 to 16 alleles (9.35 on average) with a mean heterozygosity value of 0.82 that produced successful amplifications and revealed DNA polymorphisms. Allele size varied from 95 to 290 bp. Cluster analyses showed that the studied sweet cherry genotypes were classified intofive main groups based mainly on their species characteristics and SSR data. In general, our results did not show a clear structuring of genetic variability within the Iranian diffusion area of sweet cherry, so it was not possible to draw any indications on regions of provenance delimitation. The results of this study contribute to a better understanding of sweet cherry genetic variations in Iran, thus making for more efficient programs aimed at preserving biodiversity and more rational planning of the management of reproductive material.","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 1","pages":"12-25"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34442855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PIXEL SENSIBLE LOCAL BAND ANALYSIS IN MICROSCOPIC CHROMOSOME IMAGES USING CSPA. 基于cspa的显微染色体图像的像素敏感局部带分析。

TSitologiia i genetika Pub Date : 2016-01-01

D Somasundaram

引用次数: 0

B CHROMOSOMES IN ANGIOSPERM--A REVIEW. 被子植物B染色体研究进展。

TSitologiia i genetika Pub Date : 2016-01-01

A K Datta, A Mandal, D Das, S Gupta, A Saha, R Paul, S Sengupta

引用次数: 0