面包小麦籽粒品质性状1B染色体qtl的鉴定

TSitologiia i genetika Pub Date : 2016-03-01
M Shahzad, S H Khan, A S Khan, M Sajjad, A Rehman, A I Khan
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引用次数: 0

摘要

本研究考虑了小麦籽粒品质性状、遗传多样性和标记性状关联分析的重要性。尽管研究中包含的基因型是结构化的,但在各种种子性状中发现了大量的遗传多样性。多态性程度高,范围为2 ~ 13个,平均每个位点有6.5个等位基因。用30个非连锁ssr检测群体结构,将92个基因型群体划分为3个亚群体。在1B染色体上发现了广泛的LD范围,有42个1B染色体特异性SSRs。使用混合线性模型确定标记-性状关联,其中,基于非连锁标记计算的群体结构和亲缘关系与1B个特定标记和性状数据进行协变。蛋白质、面筋含量、试验重量、面包和chapati制作品质等5个性状的8个qtl。引物WMC419 (32 cM)的蛋白质含量、试重、面包品质和Glu-B1显著相关;WMC128 (30 cM)、WMC419 (32 cM);WMC818 (17 cM)和WMC416 (44 cM)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IDENTIFICATION OF QTLs ON CHROMOSOME 1B FOR GRAIN QUALITY TRAITS IN BREAD WHEAT (TRITICUM AESTIVUM L.).

The present study was designed considering the importance of grain quality traits, genetic diversity and marker-trait association analysis in wheat. A significant amount of genetic diversity was found for various seed traits though the genotypes included in the study were found structured. The extent of polymorphism was high with a range of 2-13 alleles and average of 6.5 alleles per locus. Population structure was detected with 30 unlinked SSRs that divided the population of 92 genotypes in three sub-populations. Extensive LD extent was found on chromosome 1B with 42 SSRs specific for 1B chromosome. Marker-trait associations were determined using mixed linear model, where, population structure and kinship calculated on the basis of unlinked markers were covariated with 1B specific markers and traits data. Eight QTLs for five traits including protein, gluten contents, test weight bread and chapati making quality. Protein content, test weight, bread quality and Glu-B1 were found significantly associated with primers WMC419 (32 cM); WMC128 (30 cM), WMC419 (32 cM); WMC818 (17 cM) and WMC416 (44 cM), respectively.

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