Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophila melanogaster.

TSitologiia i genetika Pub Date : 2016-05-01
Y Yasinskiy, L V Omelyanchuk, O V Zhuk, I A Kozeretska
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Abstract

We studied a set of Drosophila melanogaster strains that could be potentially suitable for testing a variety of mutagenic factors. Their genomes contained insertions of the enhancer trap P{lacW} in which the activity of the LacZ reporter is under the control of the reparation genes’ regulatory region. We demonstrated that the beta-galactosidase reporter, which is encoded by insertion of P{lacW} element in the gene mus209, is induced by irradiation in the cells of the salivary glands and wing imaginal discs. Despite the fact that the reporting coloration is not associated with the dose of radiation treatment, we found that the induction threshold of the reporter is different for these tissues. Thus, coloration in salivary glands is detectable after the dose of 200 rad and above, whereas the imaginal discs get colored with 500 rad and above. Thereby, multiple thresholds for induction of the reporter in the various tissues allow approximating the received dose.

利用黑腹果蝇修复基因mus209的LacZ报告活性进行诱变试验。
我们研究了一组可能适合测试各种致突变因子的黑腹果蝇菌株。它们的基因组包含增强子陷阱P{lacW}的插入,其中LacZ报告基因的活性受修复基因调控区域的控制。我们证明了β -半乳糖苷酶报告基因是通过在基因mus209中插入P{lacW}元素编码的,在唾液腺和翅膀成像盘细胞中被照射诱导。尽管报告颜色与放射治疗剂量无关,但我们发现报告者对这些组织的诱导阈值是不同的。因此,在200拉德及以上的剂量下唾液腺的颜色是可检测的,而在500拉德及以上的剂量下,成像光盘的颜色是可检测的。因此,用于在各种组织中诱导报告蛋白的多个阈值允许近似接受的剂量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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