Toxicology Mechanisms and Methods最新文献

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Obesity aggravates neuroinflammatory and neurodegenerative effects of bisphenol A in female rats. 肥胖会加重双酚 A 对雌性大鼠神经炎症和神经退行性病变的影响。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-09-01 Epub Date: 2024-05-16 DOI: 10.1080/15376516.2024.2349538
Anuradha Mangla, Poonam Goswami, Bhaskar Sharma, Suramya Suramya, Garima Jindal, Mehjbeen Javed, Mohd Anas Saifi, Suhel Parvez, Tapas Chandra Nag, Sheikh Raisuddin
{"title":"Obesity aggravates neuroinflammatory and neurodegenerative effects of bisphenol A in female rats.","authors":"Anuradha Mangla, Poonam Goswami, Bhaskar Sharma, Suramya Suramya, Garima Jindal, Mehjbeen Javed, Mohd Anas Saifi, Suhel Parvez, Tapas Chandra Nag, Sheikh Raisuddin","doi":"10.1080/15376516.2024.2349538","DOIUrl":"10.1080/15376516.2024.2349538","url":null,"abstract":"<p><p>Bisphenol A (BPA), a common plasticizer, is categorized as a neurotoxic compound. Its impact on individuals exhibits sex-linked variations. Several biological and environmental factors impact the degree of toxicity. Moreover, nutritional factors have profound influence on toxicity outcome. BPA has been demonstrated to be an obesogen. However, research on the potential role of obesity as a confounding factor in BPA toxicity is lacking. We studied the neurodegenerative effects in high-fat diet (HFD)-induced obese female rats after exposure to BPA (10 mg/L <i>via</i> drinking water for 90 days). Four groups were taken in this study - Control, HFD, HFD + BPA and BPA. Cognitive function was evaluated through novel object recognition (NOR) test. Inflammatory changes in brain, and changes in hormonal level, lipid profile, glucose tolerance, oxidative stress, and antioxidants were also determined. HFD + BPA group rats showed a significant decline in memory function in NOR test. The cerebral cortex (CC) of the brain showed increased neurodegenerative changes as measured by microtubule-associated protein-2 (MAP-2) accompanied by histopathological confirmation. The increased level of neuroinflammation was demonstrated by microglial activation (Iba-1) and protein expression of nuclear factor- kappa B (NF-КB) in the brain. Obesity also caused significant (<i>p</i> < 0.05) increase in lipid peroxidation accompanied by reduced activities of antioxidant enzymes (glutathione S-transferase, catalase and glutathione peroxidase) and decrease in reduced-glutathione (<i>p</i> < 0.05) when compared to non-obese rats with BPA treatment. Overall, study revealed that obesity serves as a risk factor in the toxicity of BPA which may exacerbate the progression of neurological diseases.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"781-794"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Farnesoid X receptor modulator 12β-(m-methyl-benzoyl)-11,12-dihydro oleanolic acid represses liver fibrosis by inhibiting ERK/p38 signaling pathways. 类arnesoid X受体调节剂12β-(甲基苯甲酰基)-11,12-二氢齐墩果酸通过抑制ERK/p38信号通路抑制肝纤维化
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-09-01 Epub Date: 2024-05-14 DOI: 10.1080/15376516.2024.2349551
Yiming Li, Yunyang Bao, Simin Guo, Yang Li, Weishuo Fang, Na Zhang, Hongwei He
{"title":"Farnesoid X receptor modulator 12β-(<i>m</i>-methyl-benzoyl)-11,12-dihydro oleanolic acid represses liver fibrosis by inhibiting ERK/p38 signaling pathways.","authors":"Yiming Li, Yunyang Bao, Simin Guo, Yang Li, Weishuo Fang, Na Zhang, Hongwei He","doi":"10.1080/15376516.2024.2349551","DOIUrl":"10.1080/15376516.2024.2349551","url":null,"abstract":"<p><p>Liver fibrosis is a common pathological process in the progression of several chronic liver diseases to cirrhosis and hepatocellular carcinoma. Therefore, the development of medications that can repress the progress of liver fibrosis is essential. We discovered that initially, 12β-(<i>m</i>-methyl-benzoyl)-11,12-dihydro oleanolic acid (12d-OA), a farnesoid X receptor (FXR) modulator, possessed potential anti-fibrotic properties. Through an in-depth study, we revealed that 12d-OA not only inhibited the expression of fibrogenic markers in the LX-2 cells and HSC-T6 cells but also exhibited significant protective effects against liver injury and liver fibrosis in bile duct ligation (BDL) rats. Further exploration of its molecular mechanism indicated that 12d-OA exerted antifibrotic activity by inhibiting the extracellular signal-regulated kinase (ERK)/stress-activated protein kinase (p38) signaling pathways. Consequently, the great effects of 12d-OA <i>in vitro</i> and <i>in vivo</i> suggest that it may be a good candidate for liver fibrosis.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"795-802"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leflunomide-induced cardiac injury in adult male mice and bioinformatic approach identifying Nrf2/NF-κb signaling interplay. 来氟米特诱导成年雄性小鼠心脏损伤以及识别 Nrf2/NF-κB 信号相互作用的生物信息学方法。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-07-01 Epub Date: 2024-03-11 DOI: 10.1080/15376516.2024.2322666
Abeer A Rahman, Ann Hegazy, Lamiaa M Elabbasy, Mohamed Z Shoaeir, Tarek M Abdel-Aziz, Awad S Abbas, Heba W Z Khella, Amira H Eltrawy, Reem Alshaman, Sheka Yagub Aloyouni, Afaf A Aldahish, Sawsan A Zaitone
{"title":"Leflunomide-induced cardiac injury in adult male mice and bioinformatic approach identifying Nrf2/NF-κb signaling interplay.","authors":"Abeer A Rahman, Ann Hegazy, Lamiaa M Elabbasy, Mohamed Z Shoaeir, Tarek M Abdel-Aziz, Awad S Abbas, Heba W Z Khella, Amira H Eltrawy, Reem Alshaman, Sheka Yagub Aloyouni, Afaf A Aldahish, Sawsan A Zaitone","doi":"10.1080/15376516.2024.2322666","DOIUrl":"10.1080/15376516.2024.2322666","url":null,"abstract":"<p><p>Leflunomide (LFND) is an immunosuppressive and immunomodulatory disease-modifying antirheumatic drug (DMARD) that was approved for treating rheumatoid arthritis. LFND-induced cardiotoxicity was not fully investigated since its approval. We investigated the cardiac injury in male mice and identified the role of nuclear factor erythroid 2-related factor 2/nuclear factor-κ B (Nrf2/NF-κB) signaling. Male albino mice were assigned into five groups as control, vehicle, and LFND (2.5, 5, and 10 mg/kg). We investigated cardiac enzymes, histopathology, and the mRNA expression of Nrf2, NF-κB, BAX, and tumor necrosis factor-α (TNF-α). The bioinformatic study identified the interaction between LFND and Nrf2/NF-κB signaling; this was confirmed by amelioration in mRNA expression (0.5- to 0.34-fold decrease in Nrf2 and 2.6- to 4.61-fold increases in NF-κB genes) and increased (1.76- and 2.625-fold) serum creatine kinase (CK) and 1.38- and 2.33-fold increases in creatine kinase-MB (CK-MB). Histopathological results confirmed the dose-dependent effects of LFND on cardiac muscle structure in the form of cytoplasmic, nuclear, and vascular changes in addition to increased collagen deposits and apoptosis which were increased compared to controls especially with LFND 10 mg/kg. The current study elicits the dose-dependent cardiac injury induced by LFND administration and highlights, for the first time, dysregulation in Nrf2/NF-κB signaling.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"639-653"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Renoprotective effect of hyperin against CdCl2 prompted renal damage by activation of Nrf-2/Keap-1 ARE pathway in male mice. 金丝桃素通过激活 Nrf-2/Keap-1 ARE 通路对雄性小鼠氯化镉肾损伤的保护作用
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-07-01 Epub Date: 2024-03-20 DOI: 10.1080/15376516.2024.2329655
Iserhienrhien O Lucky, Iyoha I Aisuhuehien, Memudu E Adejoke
{"title":"Renoprotective effect of hyperin against CdCl<sub>2</sub> prompted renal damage by activation of Nrf-2/Keap-1 ARE pathway in male mice.","authors":"Iserhienrhien O Lucky, Iyoha I Aisuhuehien, Memudu E Adejoke","doi":"10.1080/15376516.2024.2329655","DOIUrl":"10.1080/15376516.2024.2329655","url":null,"abstract":"<p><strong>Objectives: </strong>This study explored the mitigating properties of hyperin (HYP) on renotoxicity induced by cadmium chloride (CdCl<sub>2</sub>).</p><p><strong>Methods: </strong>Four groups of seven male albino mice each were used in this experiment. Group 1 served as the control, receiving no treatment. Group 2 received daily oral gavage of CdCl<sub>2</sub> at 0.3 mg/kg body weight for 28 d. Group 3 received both CdCl<sub>2</sub> (0.3 mg/kg) and HYP (100 mg/kg) daily using the same administration method. Finally, Group 4 received only HYP (100 mg/kg) daily.</p><p><strong>Results: </strong>Cd exposure significantly increased kidney dysfunction markers (blood urea nitrogen and creatinine) and oxidative stress (reactive oxygen species [ROS] and malondialdehyde [MDA]). Conversely, it decreased antioxidant enzyme activities (glutathione peroxidase (GPx] and catalase [CAT]) and glutathione (GSH) levels. Nuclear factor erythroid 2-related factor 2 (Nrf-2) and antioxidant gene expression decreased, while Kelch-like ECH-associated protein 1 expression increased. Additionally, Cd exposure increased inflammatory mediators (nuclear factor kappa B, tumor necrosis factor alpha [TNF-α], interleukin-1β [IL-1β], and cyclooxygenase-2) and apoptotic markers (Bax and caspase-3), alongside decreased Bcl-2 expression and renal tissue abnormalities. Mitochondrial dysfunction manifested with diminished activities of Krebs cycle and respiratory chain enzymes, and reduced mitochondrial membrane potential. Co-treatment with HYP significantly attenuated these detrimental effects through its anti-apoptotic, antioxidant, and anti-inflammatory properties.</p><p><strong>Conclusion: </strong>HYP co-treatment significantly attenuated CdCl<sub>2</sub>-induced renal damage in mice, suggesting its potential as a protective agent against Cd-induced kidney toxicity.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"717-726"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mass spectrometric methods for evaluation of voriconazole avian pharmacokinetics and the inhibition of its cytochrome P450-induced metabolism. 评估伏立康唑禽药代动力学及其细胞色素 P450 诱导的代谢抑制的质谱方法。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-07-01 Epub Date: 2024-02-29 DOI: 10.1080/15376516.2024.2322675
Andreas F Lehner, Sharmie D Johnson, Levent Dirikolu, Margaret Johnson, John P Buchweitz
{"title":"Mass spectrometric methods for evaluation of voriconazole avian pharmacokinetics and the inhibition of its cytochrome P450-induced metabolism.","authors":"Andreas F Lehner, Sharmie D Johnson, Levent Dirikolu, Margaret Johnson, John P Buchweitz","doi":"10.1080/15376516.2024.2322675","DOIUrl":"10.1080/15376516.2024.2322675","url":null,"abstract":"<p><p>Invasive fungal aspergillosis is a leading cause of morbidity and mortality in many species including avian species such as common ravens (<i>Corvus corax</i>). Methods were developed for mass spectral determination of voriconazole in raven plasma as a means of determining pharmacokinetics of this antifungal agent. Without further development, GC/MS/MS (gas chromatography-tandem quadrupole mass spectrometry) proved to be inferior to LC/MS/MS (liquid chromatography-tandem quadrupole mass spectrometry) for measurement of voriconazole levels in treated raven plasma owing to numerous heat-induced breakdown products despite protection of voriconazole functional groups with trimethylsilyl moieties. LC/MS/MS measurement revealed in multi-dosing experiments that the ravens were capable of rapid or ultrarapid metabolism of voriconazole. This accounted for the animals' inability to raise the drug into the therapeutic range regardless of dosing regimen unless cytochrome P450 (CYP) inhibitors were included. Strategic selection of CYP inhibitors showed that of four selected compounds including cimetidine, enrofloxacin and omeprazole, only ciprofloxacin (Cipro) was able to maintain voriconazole levels in the therapeutic range until the end of the dosing period. The optimal method of administration involved maintenance doses of voriconazole at 6 mg/kg and ciprofloxacin at 20 mg/kg. Higher doses of voriconazole such as 18 mg/kg were also tenable without apparent induction of toxicity. Although most species employ CYP2C19 to metabolize voriconazole, it was necessary to speculate that voriconazole might be subject to metabolism by CYP1A2 in the ravens to explain the utility of ciprofloxacin, a previously unknown enzymatic route. Finally, despite its widespread catalog of CYP inhibitions including CYP1A2 and CYP2C19, cimetidine may be inadequate at enhancing voriconazole levels owing to its known effects on raising gastric pH, a result that may limit voriconazole solubility.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"654-668"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analyzing the impact of synthetic and natural steroids: a study of cytochrome P450 metabolism, morphological alterations through metabolomics, and histopathological Examination. 分析合成和天然类固醇的影响:细胞色素 P450 代谢、代谢组学形态改变和组织病理学检查研究。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-07-01 Epub Date: 2024-02-27 DOI: 10.1080/15376516.2024.2322006
Esat Mahmut Kocaman, Onur Şenol, Serkan Yıldırım, Muhammed Atamanalp, Sinan Özcan, İsmail Bolat, Arzu Ucar, Metin Kiliçlioğlu, Veysel Parlak, Mehmet Takkac, Gonca Alak
{"title":"Analyzing the impact of synthetic and natural steroids: a study of cytochrome P450 metabolism, morphological alterations through metabolomics, and histopathological Examination.","authors":"Esat Mahmut Kocaman, Onur Şenol, Serkan Yıldırım, Muhammed Atamanalp, Sinan Özcan, İsmail Bolat, Arzu Ucar, Metin Kiliçlioğlu, Veysel Parlak, Mehmet Takkac, Gonca Alak","doi":"10.1080/15376516.2024.2322006","DOIUrl":"10.1080/15376516.2024.2322006","url":null,"abstract":"<p><p>This study focuses on the comparative metabolic profiling and effects of two steroid types: natural and synthetic, specifically 17α-methyl testosterone (17α-MT) at varying concentrations (1.5, 2, and 3 mg/kg) in rainbow trout (<i>Oncorhynchus mykiss</i>). Over a 75-day feeding trial, growth metrics, such as feed efficiency, daily specific growth, live weight gain, total weight gain, and survival rate were systematically monitored every 15 days. At the end of the feeding trial, histopathology, immunohistochemistry, and metabolome analyses were performed in the high-concentration groups (3 mg/kg natural and 3 mg/kg synthetic), in which the lowest survival rate was determined. Key findings reveal that the type of hormone significantly influences growth parameters. While some natural steroids enhanced certain growth aspects, synthetic variants often yielded better results. The metabolomic analysis highlighted significant shifts in the metabolism of tryptophan, purine, folate, primary bile acids, phosphonates, phosphinates, and xenobiotics <i>via</i> cytochrome P450 pathways. Histopathologically, the natural hormone groups showed similar testicular, hepatic, muscular, gill, cerebral, renal, and intestinal tissue structures to the control, with minor DNA damage and apoptosis observed through immunohistochemistry. Conversely, the synthetic hormone groups exhibited moderate DNA damage and mild degenerative and necrotic changes in histopathology.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"628-638"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroprotective effect of taxifolin against aluminum chloride-induced dementia and pathological alterations in the brain of rats: possible involvement of toll-like receptor 4. taxifolin对氯化铝诱发的大鼠痴呆和脑病理改变的神经保护作用:收费样受体 4 的可能参与。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-07-01 Epub Date: 2024-03-25 DOI: 10.1080/15376516.2024.2329653
Bhagawati Saxena, Pragnesh Parmar, Heena Chauhan, Pooja Singh, Ashok Kumar Datusalia, Vivek Kumar Vyas, Nagja Tripathi, Jigna Shah
{"title":"Neuroprotective effect of taxifolin against aluminum chloride-induced dementia and pathological alterations in the brain of rats: possible involvement of toll-like receptor 4.","authors":"Bhagawati Saxena, Pragnesh Parmar, Heena Chauhan, Pooja Singh, Ashok Kumar Datusalia, Vivek Kumar Vyas, Nagja Tripathi, Jigna Shah","doi":"10.1080/15376516.2024.2329653","DOIUrl":"10.1080/15376516.2024.2329653","url":null,"abstract":"<p><p>Aluminum (Al) overexposure damages various organ systems, especially the nervous system. Regularly administered aluminum chloride (AlCl<sub>3</sub>) to rats causes dementia and pathophysiological alterations linked to Alzheimer's disease (AD). Taxifolin's neuroprotective effects against AlCl<sub>3</sub>-induced neurotoxicity <i>in vitro</i> and <i>in vivo</i> studies were studied. Taxifolin (0.1, 0.3, 1, 3, and 10 μM) was tested against AlCl<sub>3</sub> (5 mM)-induced neurotoxicity in C6 and SH-SY5Y cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Additionally, neural morphology was examined by confocal microscopy. Additionally, taxifolin's mode of binding with the co-receptor of toll-like receptor 4 (TLR4), human myeloid differentiation-2 (<i>h</i>MD-2) was investigated. AlCl<sub>3</sub> (25 mg/kg/d, <i>i.p.</i>) was administered to rats for 14 d, and from the eighth day, taxifolin (1, 2, and 5 mg/kg/d, <i>i.p.</i>) was given along with AlCl<sub>3</sub>. This study assessed memory impairment using the Morris water maze, plus maze, and pole tests. This study also performed measurement of oxidant (malondialdehyde [MDA] and nitrite), antioxidant (reduced glutathione), and inflammatory (myeloperoxidase [MPO] activity, TLR4 expression) parameters in rats' brain in addition to histopathology. The docking score for taxifolin with <i>h</i>MD-2 was found to be -4.38 kcal/mol. Taxifolin treatment reduced the neurotoxicity brought on by AlCl<sub>3</sub> in both C6 and SH-SY5Y cells. Treatment with 10 μM taxifolin restored AlCl<sub>3</sub>-induced altered cell morphology. AlCl<sub>3</sub> administration caused memory loss, oxidative stress, inflammation (increased MPO activity and TLR4 expression), and brain atrophy. Taxifolin treatment significantly improved the AlCl<sub>3</sub>-induced memory impairment. Taxifolin treatment also mitigated the histopathological and neurochemical consequences of repeated AlCl<sub>3</sub> administration in rats. Thus, taxifolin may protect the brain against AD.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"703-716"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A trend over time study of hepatic Farnesoid-X-activated receptor and its downstream targets modulation by valproic acid in mice 丙戊酸对小鼠肝脏法尼类固醇-X-激活受体及其下游靶点调节作用的随时间变化趋势研究
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-06-18 DOI: 10.1080/15376516.2024.2364192
Amir Saamaan Fattahi, Azadeh Khalili, Seyed Ali Hashemi, Parvaneh Najafizadeh, Roham Mazloom, Sara Khodayar, Gholamreza Bayat
{"title":"A trend over time study of hepatic Farnesoid-X-activated receptor and its downstream targets modulation by valproic acid in mice","authors":"Amir Saamaan Fattahi, Azadeh Khalili, Seyed Ali Hashemi, Parvaneh Najafizadeh, Roham Mazloom, Sara Khodayar, Gholamreza Bayat","doi":"10.1080/15376516.2024.2364192","DOIUrl":"https://doi.org/10.1080/15376516.2024.2364192","url":null,"abstract":"Valproic acid (VA) is a broad-spectrum anticonvulsant agent that acts through several molecular mechanisms to control different types of seizures. The main concern of the drug is its liver toxicity...","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":"16 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering molecular basis of pesticide-induced recurrent pregnancy loss: insights from transcriptomics analysis. 解密农药诱发复发性妊娠失败的分子基础:转录组学分析的启示。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-06-01 Epub Date: 2024-01-31 DOI: 10.1080/15376516.2024.2307975
Muhammad Luthfi, R B Pandey, Yong-Chao Su, Pornthep Sompornpisut
{"title":"Deciphering molecular basis of pesticide-induced recurrent pregnancy loss: insights from transcriptomics analysis.","authors":"Muhammad Luthfi, R B Pandey, Yong-Chao Su, Pornthep Sompornpisut","doi":"10.1080/15376516.2024.2307975","DOIUrl":"10.1080/15376516.2024.2307975","url":null,"abstract":"<p><p>Recent studies have revealed a notable connection between pesticide exposure and Recurrent Pregnancy Loss (RPL), yet the precise molecular underpinning of this toxicity remains elusive. Through the alignment of Differentially Expressed Genes (DEGs) of healthy and RPL patients with the target genes of 9 pesticide components, we identified a set of 12 genes responsible for RPL etiology. Interestingly, biological process showed that besides RPL, those 12 genes also associated with preeclampsia and cardiovascular disease. Enrichment analysis showed the engagement of these genes associated with essential roles in the molecular transport of small molecules, as well as the aldosterone-regulated sodium reabsorption, endocrine and other factor-regulated calcium reabsorption, mineral absorption, ion homeostasis, and ion transport by P-type ATPases. Notably, the crosstalk targets between pesticide components played crucial roles in influencing RPL results, suggesting a role in attenuating pesticide agents that contribute to RPL. It is important to note that non-significant concentration of the pesticide components observed in both control and RPL samples should not prematurely undermine the potential for pesticides to induce RPL in humans. This study emphasizes the complexity of pesticide induced RPL and highlights avenues for further research and precautionary measures.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"527-544"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139642997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evening primrose oil enriched with gamma linolenic acid and D/L-alpha tocopherol acetate attenuated carbon tetrachloride-induced hepatic injury model in male rats via TNF-α, IL-1β, and IL-6 pathway. 富含γ-亚麻酸和D/L-α-生育酚醋酸酯的月见草油通过TNF-α、IL1 β和IL6途径减轻了四氯化碳诱导的雄性大鼠肝损伤模型。
IF 3.2 4区 医学
Toxicology Mechanisms and Methods Pub Date : 2024-06-01 Epub Date: 2024-01-09 DOI: 10.1080/15376516.2023.2301357
Heba Nageh Gad El-Hak, Safaa M Kishk, Heba M A Abdelrazek
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