Toxicology Mechanisms and Methods最新文献_第10页

Investigation of the effects of Theranekron and Sorafenib treatments on carcinogenesis, apoptosis and biochemical profile in hepatocellular carcinoma in rats 研究 Theranekron 和 Sorafenib 治疗对大鼠肝细胞癌的癌变、凋亡和生化指标的影响

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-04-05 DOI: 10.1080/15376516.2024.2332909

Serdar Vanli, Firuze Kurtoglu, Beyza S. Alan, Gokhan Akcakavak, Ozgur Ozdemir

引用次数: 0

Flow-through imaging and automated analysis of oil-exposed early stage Atlantic cod (Gadus morhua) 对暴露于油中的早期大西洋鳕鱼（Gadus morhua）进行流动成像和自动分析

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-04-04 DOI: 10.1080/15376516.2024.2338389

David R. Williamson, Emlyn J. Davies, Martin Ludvigsen, Bjørn Henrik Hansen

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Isoflurane pretreatment protects against myocardial ischemia/reperfusion injury via mediating lncRNA CASC15/miR-542-3p axis 异氟烷预处理通过介导lncRNA CASC15/miR-542-3p轴防止心肌缺血再灌注损伤

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-04-04 DOI: 10.1080/15376516.2024.2327057

Xiaoyi Wang, Yueping Wang, Yawei Yuan, Long Wang, Dawei Zhang

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Does the accounting of the local symmetry fragments in quasi-SMILES improve the predictive potential of the QSAR models of toxicity towards tadpoles? 在准 SMILES 中考虑局部对称性片段是否能提高 QSAR 模型对蝌蚪毒性的预测潜力？

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-04-04 DOI: 10.1080/15376516.2024.2332617

Alla P. Toropova, Andrey A. Toropov, Alessandra Roncaglioni, Emilio Benfenati

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Drug reactive metabolite-induced hepatotoxicity: A Comprehensive review 药物活性代谢物诱发的肝毒性：全面回顾

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-19 DOI: 10.1080/15376516.2024.2332613

Piyush Mahajan, Mahesh Palkar, Ravindra Babu Pingili

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Quercetin induces ferroptosis by inactivating mTOR/S6KP70 pathway in oral squamous cell carcinoma 槲皮素通过灭活口腔鳞状细胞癌中的 mTOR/S6KP70 通路诱导铁变态反应

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-04 DOI: 10.1080/15376516.2024.2325989

Ya-wen Zhu, Chun-lei Liu, Xiao-mei Li, Yu Shang

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Subjective Biosafety Assessment of Bisdemethoxycurcumin from the rhizomes of Curcuma longa 从莪术根茎中提取双去甲氧基姜黄素的主观生物安全性评估

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-04 DOI: 10.1080/15376516.2024.2326000

Muhammed Majeed, Sarang Bani, Anjali Pandey, Muhamad Ibrahim A, Smitha Thazhathidath

引用次数: 0

Bioinformatics analysis of differentially expressed genes related to ischemia and hypoxia in spinal cord injury and construction of miRNA-mRNA or mRNA-transcription factor interaction network. 脊髓损伤缺血缺氧相关差异表达基因的生物信息学分析及miRNA-mRNA或mrna -转录因子相互作用网络的构建。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2024-01-03 DOI: 10.1080/15376516.2023.2286363

Lijuan Zhu, Na Gao, Zhibo Zhu, Shiping Zhang, Xi Li, Jing Zhu

{"title":"Bioinformatics analysis of differentially expressed genes related to ischemia and hypoxia in spinal cord injury and construction of miRNA-mRNA or mRNA-transcription factor interaction network.","authors":"Lijuan Zhu, Na Gao, Zhibo Zhu, Shiping Zhang, Xi Li, Jing Zhu","doi":"10.1080/15376516.2023.2286363","DOIUrl":"10.1080/15376516.2023.2286363","url":null,"abstract":"Background: Previous studies show that spinal cord ischemia and hypoxia is an important cause of spinal cord necrosis and neurological loss. Therefore, the study aimed to identify genes related to ischemia and hypoxia after spinal cord injury (SCI) and analyze their functions, regulatory mechanism, and potential in regulating immune infiltration.Methods: The expression profiles of GSE5296, GSE47681, and GSE217797 were downloaded from the Gene Expression Omnibus database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to determine the function and pathway enrichment of ischemia- and hypoxia-related differentially expressed genes (IAHRDEGs) in SCI. LASSO model was constructed, and support vector machine analysis was used to identify key genes. The diagnostic values of key genes were evaluated using decision curve analysis and receiver operating characteristic curve analysis. The interaction networks of miRNAs-IAHRDEGs and IAHRDEGs-transcription factors were predicted and constructed with the ENCORI database and Cytoscape software. CIBERSORT algorithm was utilized to analyze the correlation between key gene expression and immune cell infiltration.Results: There were 27 IAHRDEGs identified to be significantly expressed in SCI at first. These genes were mostly significantly enriched in wound healing function and the pathway associated with lipid and atherosclerosis. Next, five key IAHRDEGs (Abca1, Casp1, Lpl, Procr, Tnfrsf1a) were identified and predicted to have diagnostic value. Moreover, the five key genes are closely related to immune cell infiltration.Conclusion: Abca1, Casp1, Lpl, Procr, and Tnfrsf1a may promote the pathogenesis of ischemic or hypoxic SCI by regulating vascular damage, inflammation, and immune infiltration.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"300-318"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug target genes and molecular mechanism investigation in isoflurane-induced anesthesia based on WGCNA and machine learning methods. 基于WGCNA和机器学习方法的异氟醚诱导麻醉的药物靶基因及分子机制研究

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-12-06 DOI: 10.1080/15376516.2023.2286619

Honglei Yuan, Shengqiang Yang, Peng Han, Mingya Sun, Chao Zhou

{"title":"Drug target genes and molecular mechanism investigation in isoflurane-induced anesthesia based on WGCNA and machine learning methods.","authors":"Honglei Yuan, Shengqiang Yang, Peng Han, Mingya Sun, Chao Zhou","doi":"10.1080/15376516.2023.2286619","DOIUrl":"10.1080/15376516.2023.2286619","url":null,"abstract":"Purpose: This study sought to identify drug target genes and their associated molecular mechanisms during isoflurane-induced anesthesia in clinical applications.Methods: Microarray data (ID: GSE64617; isoflurane-treated vs. normal samples) were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were screened and hub genes were investigated using weighted correlation network analysis (WGCNA). Protein-protein interactions (PPIs) were constructed among the co-DEGs (common genes between DEGs and hub genes), followed by functional enrichment analyses. Then, three machine learning methods were used to reveal drug targets, followed by validation, nomogram analysis, and gene set enrichment analysis. Finally, an miRNA-target network was constructed.Results: A total of 686 DEGs were identified between the two groups-of which, 183 DEGs integrated with genes revealed by WCGNA were identified as co-genes. These genes, including contactin-associated protein 1 (CNTNAP1), are mainly involved in functions such as action potentials. PPI network analysis revealed three models, with the machine learning analysis exploring four drug target genes: A2H, FAM155B, SCARF2, and SDR16C5. ROC and nomogram analyses demonstrated the ideal diagnostic value of these target genes. Finally, miRNA-mRNA pairs were constructed based on the four mRNAs and associated 174 miRNAs.Conclusion: FA2H, FAM155B, SCARF2, and SDR16C5 may be novel drug target genes for isoflurane-induced anesthesia. CNTNAP1 may participate in the progression of isoflurane-induced anesthesia via its action potential function.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"319-333"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stability of cyanide in human biological samples. A systematic review, meta-analysis and determination of cyanide (GC-QqQ-MS/MS) in an authentic casework 7 years after fatal intoxication. 氰化物在人体生物样品中的稳定性。致死性中毒7年后的真实病例的系统回顾、荟萃分析和氰化物测定(gc - qq -MS/MS)

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-11-28 DOI: 10.1080/15376516.2023.2280212

Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot

{"title":"The stability of cyanide in human biological samples. A systematic review, meta-analysis and determination of cyanide (GC-QqQ-MS/MS) in an authentic casework 7 years after fatal intoxication.","authors":"Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot","doi":"10.1080/15376516.2023.2280212","DOIUrl":"10.1080/15376516.2023.2280212","url":null,"abstract":"A 30 year old man was found with no signs of life in front of the house. The cyanide concentration in blood and urine was determined five years after the man's death. What is more, a stability study was conducted for 730 days in an authentic casework blood sample. Sample preparation procedure included precipitation with methanol:water mixture, solid phase extraction (SPE) and derivatization with the use of PFB-Br (pentafluorobenzyl bromide). The sample was analyzed using GC-QqQ-MS/MS (gas chromatopraphy coupled with tandem mass spectrometry) isotope dilution method. Separation was done using a SH-RXI-5MS column (30 m x 0.25 mm, 0.25 µm). Detection of PFB-CN and PFB-13CN was achieved using a triple-quadrupole mass spectrometer with an electron ionization (EI) ion source in multiple reaction monitoring (MRM) mode. After 5 years from the man's death, cyanide concentration was: 1900 ng/mL in blood and 500 ng/mL in urine. Stability study performed in an authentic blood sample 6 and 7 years after the man's death revealed cyanide concentrations of 1898.2 ng/mL and 1618.7 ng/mL, respectively. While spectrophotometric and colorimetric methods recorded both decrease and increase in cyanide concentration over time, newer chromatographic methods mainly indicate a decrease. The studies presented in this paper seem to confirm this trend. However, in order to interpretate the results of cyanide concentration in biological material reliably, more research is still necessary.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"271-282"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138446295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0