Toxicology Mechanisms and Methods最新文献_第9页

Drug reactive metabolite-induced hepatotoxicity: A Comprehensive review 药物活性代谢物诱发的肝毒性：全面回顾

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-19 DOI: 10.1080/15376516.2024.2332613

Piyush Mahajan, Mahesh Palkar, Ravindra Babu Pingili

引用次数: 0

Quercetin induces ferroptosis by inactivating mTOR/S6KP70 pathway in oral squamous cell carcinoma 槲皮素通过灭活口腔鳞状细胞癌中的 mTOR/S6KP70 通路诱导铁变态反应

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-04 DOI: 10.1080/15376516.2024.2325989

Ya-wen Zhu, Chun-lei Liu, Xiao-mei Li, Yu Shang

引用次数: 0

Subjective Biosafety Assessment of Bisdemethoxycurcumin from the rhizomes of Curcuma longa 从莪术根茎中提取双去甲氧基姜黄素的主观生物安全性评估

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-04 DOI: 10.1080/15376516.2024.2326000

Muhammed Majeed, Sarang Bani, Anjali Pandey, Muhamad Ibrahim A, Smitha Thazhathidath

引用次数: 0

Bioinformatics analysis of differentially expressed genes related to ischemia and hypoxia in spinal cord injury and construction of miRNA-mRNA or mRNA-transcription factor interaction network. 脊髓损伤缺血缺氧相关差异表达基因的生物信息学分析及miRNA-mRNA或mrna -转录因子相互作用网络的构建。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2024-01-03 DOI: 10.1080/15376516.2023.2286363

Lijuan Zhu, Na Gao, Zhibo Zhu, Shiping Zhang, Xi Li, Jing Zhu

{"title":"Bioinformatics analysis of differentially expressed genes related to ischemia and hypoxia in spinal cord injury and construction of miRNA-mRNA or mRNA-transcription factor interaction network.","authors":"Lijuan Zhu, Na Gao, Zhibo Zhu, Shiping Zhang, Xi Li, Jing Zhu","doi":"10.1080/15376516.2023.2286363","DOIUrl":"10.1080/15376516.2023.2286363","url":null,"abstract":"Background: Previous studies show that spinal cord ischemia and hypoxia is an important cause of spinal cord necrosis and neurological loss. Therefore, the study aimed to identify genes related to ischemia and hypoxia after spinal cord injury (SCI) and analyze their functions, regulatory mechanism, and potential in regulating immune infiltration.Methods: The expression profiles of GSE5296, GSE47681, and GSE217797 were downloaded from the Gene Expression Omnibus database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to determine the function and pathway enrichment of ischemia- and hypoxia-related differentially expressed genes (IAHRDEGs) in SCI. LASSO model was constructed, and support vector machine analysis was used to identify key genes. The diagnostic values of key genes were evaluated using decision curve analysis and receiver operating characteristic curve analysis. The interaction networks of miRNAs-IAHRDEGs and IAHRDEGs-transcription factors were predicted and constructed with the ENCORI database and Cytoscape software. CIBERSORT algorithm was utilized to analyze the correlation between key gene expression and immune cell infiltration.Results: There were 27 IAHRDEGs identified to be significantly expressed in SCI at first. These genes were mostly significantly enriched in wound healing function and the pathway associated with lipid and atherosclerosis. Next, five key IAHRDEGs (Abca1, Casp1, Lpl, Procr, Tnfrsf1a) were identified and predicted to have diagnostic value. Moreover, the five key genes are closely related to immune cell infiltration.Conclusion: Abca1, Casp1, Lpl, Procr, and Tnfrsf1a may promote the pathogenesis of ischemic or hypoxic SCI by regulating vascular damage, inflammation, and immune infiltration.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"300-318"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug target genes and molecular mechanism investigation in isoflurane-induced anesthesia based on WGCNA and machine learning methods. 基于WGCNA和机器学习方法的异氟醚诱导麻醉的药物靶基因及分子机制研究

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-12-06 DOI: 10.1080/15376516.2023.2286619

Honglei Yuan, Shengqiang Yang, Peng Han, Mingya Sun, Chao Zhou

{"title":"Drug target genes and molecular mechanism investigation in isoflurane-induced anesthesia based on WGCNA and machine learning methods.","authors":"Honglei Yuan, Shengqiang Yang, Peng Han, Mingya Sun, Chao Zhou","doi":"10.1080/15376516.2023.2286619","DOIUrl":"10.1080/15376516.2023.2286619","url":null,"abstract":"Purpose: This study sought to identify drug target genes and their associated molecular mechanisms during isoflurane-induced anesthesia in clinical applications.Methods: Microarray data (ID: GSE64617; isoflurane-treated vs. normal samples) were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were screened and hub genes were investigated using weighted correlation network analysis (WGCNA). Protein-protein interactions (PPIs) were constructed among the co-DEGs (common genes between DEGs and hub genes), followed by functional enrichment analyses. Then, three machine learning methods were used to reveal drug targets, followed by validation, nomogram analysis, and gene set enrichment analysis. Finally, an miRNA-target network was constructed.Results: A total of 686 DEGs were identified between the two groups-of which, 183 DEGs integrated with genes revealed by WCGNA were identified as co-genes. These genes, including contactin-associated protein 1 (CNTNAP1), are mainly involved in functions such as action potentials. PPI network analysis revealed three models, with the machine learning analysis exploring four drug target genes: A2H, FAM155B, SCARF2, and SDR16C5. ROC and nomogram analyses demonstrated the ideal diagnostic value of these target genes. Finally, miRNA-mRNA pairs were constructed based on the four mRNAs and associated 174 miRNAs.Conclusion: FA2H, FAM155B, SCARF2, and SDR16C5 may be novel drug target genes for isoflurane-induced anesthesia. CNTNAP1 may participate in the progression of isoflurane-induced anesthesia via its action potential function.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"319-333"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stability of cyanide in human biological samples. A systematic review, meta-analysis and determination of cyanide (GC-QqQ-MS/MS) in an authentic casework 7 years after fatal intoxication. 氰化物在人体生物样品中的稳定性。致死性中毒7年后的真实病例的系统回顾、荟萃分析和氰化物测定(gc - qq -MS/MS)

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-11-28 DOI: 10.1080/15376516.2023.2280212

Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot

{"title":"The stability of cyanide in human biological samples. A systematic review, meta-analysis and determination of cyanide (GC-QqQ-MS/MS) in an authentic casework 7 years after fatal intoxication.","authors":"Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot","doi":"10.1080/15376516.2023.2280212","DOIUrl":"10.1080/15376516.2023.2280212","url":null,"abstract":"A 30 year old man was found with no signs of life in front of the house. The cyanide concentration in blood and urine was determined five years after the man's death. What is more, a stability study was conducted for 730 days in an authentic casework blood sample. Sample preparation procedure included precipitation with methanol:water mixture, solid phase extraction (SPE) and derivatization with the use of PFB-Br (pentafluorobenzyl bromide). The sample was analyzed using GC-QqQ-MS/MS (gas chromatopraphy coupled with tandem mass spectrometry) isotope dilution method. Separation was done using a SH-RXI-5MS column (30 m x 0.25 mm, 0.25 µm). Detection of PFB-CN and PFB-13CN was achieved using a triple-quadrupole mass spectrometer with an electron ionization (EI) ion source in multiple reaction monitoring (MRM) mode. After 5 years from the man's death, cyanide concentration was: 1900 ng/mL in blood and 500 ng/mL in urine. Stability study performed in an authentic blood sample 6 and 7 years after the man's death revealed cyanide concentrations of 1898.2 ng/mL and 1618.7 ng/mL, respectively. While spectrophotometric and colorimetric methods recorded both decrease and increase in cyanide concentration over time, newer chromatographic methods mainly indicate a decrease. The studies presented in this paper seem to confirm this trend. However, in order to interpretate the results of cyanide concentration in biological material reliably, more research is still necessary.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"271-282"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138446295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple protocol for estimating the acute toxicity of unresolved polar compounds from field-weathered oils. 估算野外风化油类中未溶解极性化合物急性毒性的简单方案。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2024-02-20 DOI: 10.1080/15376516.2024.2310003

Lisbet Sørensen, Trond R Størseth, Dag Altin, Trond Nordtug, Liv-Guri Faksness, Bjørn Henrik Hansen

{"title":"A simple protocol for estimating the acute toxicity of unresolved polar compounds from field-weathered oils.","authors":"Lisbet Sørensen, Trond R Størseth, Dag Altin, Trond Nordtug, Liv-Guri Faksness, Bjørn Henrik Hansen","doi":"10.1080/15376516.2024.2310003","DOIUrl":"10.1080/15376516.2024.2310003","url":null,"abstract":"Crude oil spilled at sea is chemically altered through environmental processes such as dissolution, biodegradation, and photodegradation. Transformation of hydrocarbons to oxygenated species increases water-solubility. Metabolites and oxidation products largely remain uncharacterized by common analytical methods but may be more bioavailable to aquatic organisms. Studies have shown that unresolved (i.e. unidentified) polar compounds ('UPCs') may constitute > 90% of the water-accommodated fraction (WAF) of heavily weathered crude oils, but still there is a paucity of information characterizing their toxicological significance in relation to other oil-derived toxicants. In this study, low-energy WAFs (no droplets) were generated from two field-weathered oils (collected during the 2010 Deepwater Horizon incident) and their polar fractions were isolated through fractionation. To allow establishment of thresholds for acute toxicity (LC50) of the dissolved and polar fraction of field collected oils, we concentrated both WAFs and polar fractions to beyond field-documented concentrations, and the acute toxicity of both to the marine copepod Acartia tonsa was measured and compared to the toxicity of the native WAF (non-concentrated). The difference in toxic units (TUs) between the total of the mixture and of identified compounds of known toxicity (polycyclic aromatic hydrocarbons [PAHs] and alkyl phenols) in both WAF and polar fractions was used to estimate the contribution of the UPC to overall toxicity. This approach identified that UPC had a similar contribution to toxicity as identified compounds within the WAFs of the field-weathered oils. This signifies the relative importance of polar compounds when assessing environmental impacts of spilled and weathered oil.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"245-255"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of genetic polymorphisms on genotoxicity in workers occupationally exposed to pesticides: a systematic review. 遗传多态性对职业农药暴露工人遗传毒性的影响:系统综述。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-11-20 DOI: 10.1080/15376516.2023.2280806

Thiago Guedes Pinto, Glenda Nicioli da Silva, Ana Claudia Muniz Renno, Daisy Maria Favero Salvadori, Daniel Araki Ribeiro

{"title":"The impact of genetic polymorphisms on genotoxicity in workers occupationally exposed to pesticides: a systematic review.","authors":"Thiago Guedes Pinto, Glenda Nicioli da Silva, Ana Claudia Muniz Renno, Daisy Maria Favero Salvadori, Daniel Araki Ribeiro","doi":"10.1080/15376516.2023.2280806","DOIUrl":"10.1080/15376516.2023.2280806","url":null,"abstract":"In a world with a rising use of pesticides, these chemicals, although designed to effectively control pests, pose potential threats to the environment and non-target organisms, including humans. Thus, this systematic review aims to investigate a possible association between genetic polymorphisms and susceptibility and genotoxicity in individuals occupationally exposed to pesticides. This review was conducted following the 2020 Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. A total of 14 carefully selected studies were thoroughly analyzed by two reviewers, who assigned scores based on previously set evaluation criteria. This study classified over half of the chosen studies as having moderate or strong quality, observing a correlation between certain genetic polymorphisms involved in xenobiotic metabolism and genotoxicity in workers exposed to pesticides. Results suggest that the genes associated with xenobiotic metabolism play a substantial role in determining individuals' susceptibility to genomic damage due to pesticide exposure, affecting both their peripheral blood and oral mucosa. This implies that individuals with specific genotypes may experience increased or decreased levels of DNA damage when exposed to these chemicals.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"237-244"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138048001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-monotonic effects of Bisphenol A Dimethacrylate on male mouse reproductive system and fertility leads to impaired conceptive performance. 双酚A二甲基丙烯酸酯对雄性小鼠生殖系统和生育能力的非单调效应导致受孕能力受损。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-11-15 DOI: 10.1080/15376516.2023.2279723

Homa Darmani, Maysoon M A Alkhatib

{"title":"Non-monotonic effects of Bisphenol A Dimethacrylate on male mouse reproductive system and fertility leads to impaired conceptive performance.","authors":"Homa Darmani, Maysoon M A Alkhatib","doi":"10.1080/15376516.2023.2279723","DOIUrl":"10.1080/15376516.2023.2279723","url":null,"abstract":"As an estrogenic agent, Bisphenol A Dimethacrylate (Bis-DMA) may incite alterations in both the reproductive tract and the neuroendocrine axis, and thus have the potential to affect the proper development, maturity and conceptive performance in animals. We investigated the consequences of 14 weeks of exposure to different concentrations of Bis-DMA on male mouse conceptive performance. Male mice were exposed to Bis-DMA (0, 0.1 mg/L, 1.0 mg/L or 10 mg/L) via drinking water, and the effects on fertility, reproductive organ weights, reproductive hormone levels, sperm counts and testicular histology were assessed. We clearly demonstrate that prolonged exposure of male mice to Bis-DMA negatively affects fertility and reproduction causing significant reductions in sperm counts, non-monotonic effects on serum LH and testosterone levels, increased seminal vesicle weights, lower number of embryonic implantations and viable fetuses, as well as, increased embryonal resorptions in females mated by Bis-DMA treated males. Furthermore, Bis-DMA caused abnormalities in testicular infrastructure with atrophic seminiferous tubules exhibiting intraepithelial vacuolization and disorganization, loss and shedding of germ cells into the lumen, and presence of apoptotic cells. Our data collectively suggest that Bis-DMA adversely affects male fertility and reproduction by interference with normal hormone signaling in the testis, inducing changes in testicular infrastructure and ultimately leading to impaired reproductive function and fertility.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"262-270"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental pollutant sodium omadine: toxic effects in zebra fish (Danio rerio). 环境污染物奥马丁钠:对斑马鱼的毒性作用。

IF 3.2 4区医学

Toxicology Mechanisms and Methods Pub Date : 2024-03-01 Epub Date: 2023-11-14 DOI: 10.1080/15376516.2023.2279717

İlknur Yılmaz Sezer, Gülsüm Koçak, Rabia Tural, Aysel Çağlan Günal, Aylin Sepici Dinçel

{"title":"Environmental pollutant sodium omadine: toxic effects in zebra fish (Danio rerio).","authors":"İlknur Yılmaz Sezer, Gülsüm Koçak, Rabia Tural, Aysel Çağlan Günal, Aylin Sepici Dinçel","doi":"10.1080/15376516.2023.2279717","DOIUrl":"10.1080/15376516.2023.2279717","url":null,"abstract":"In recent years one of the most striking results of over-population and consumption activities in the world is the rapid increase in environmental pollutants. Environmental pollutants, one of the harmful consequences of technological and modern life, threaten the health of people and other living organisms. In this study, we aimed to determine the effects of sodium omadine (NaOM) on superoxide dismutase enzyme (SOD) activity as an antioxidant and on 8-OHdG levels as oxidative DNA damage in zebrafish. Zebrafish, obtained from the aquarium fish producer, were stocked in experimental aquariums to ensure their adaptation period to the experimental conditions 15 days before the experiment. The fish were exposed to 1 ug/L and 5 ug/L concentrations of NaOM for 24, 72, and 96 h. SOD enzyme activity (U/100 mg tissue) and 8-OHdG (pg/100 mg tissue) were measured using commercial kits. The statistically significant differences in tissue SOD levels and data for DNA damage between the groups were determined as time and dose-dependent (p < 0.05). Biocidal products are environmental pollutants that cause changes in antioxidant enzyme activities, especially in non-target organisms. Marine pollution and the degradation of ecosystems directly affect people, and the results of the study offer awareness of health problems, environmental pollution, and marine pollution.","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"256-261"},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107592334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0