ACS Omega最新文献

{"title":"Effects of pH and MgSO4 on Lignin Redeposition and Retention during Brownstock Washing","authors":"Eashwara Raju Senthilkumar,&nbsp;Maria Sofia Suarez,&nbsp;Fredrik Wernersson Brodin,&nbsp;Tomas Vikström,&nbsp;Gunnar Henriksson,&nbsp;Olena Sevastyanova","doi":"10.1021/acsomega.6c06072","DOIUrl":"https://doi.org/10.1021/acsomega.6c06072","url":null,"abstract":"<p>Process closure in kraft pulp mills requires increasedrecyclingof washing liquors, which can alter liquor composition and promotelignin redeposition during brownstock washing. This study investigatesthe effect of pH and magnesium ions (Mg²⁺) on lignin retentionand removal from pulp fibers in controlled washing experiments. Unbleachedand bleached softwood kraft pulps were washed with industrial liquorscollected from different stages of the kraft process at a Swedishmill. Lignin redeposition and retention were assessed using kappanumber, brightness, and alkaline leaching. Within the alkaline pHrange investigated, decreasing pH was generally associated with increasedlignin retention on the fibers. NaOH addition improved lignin removal,but the effect plateaued beyond a liquor-dependent threshold. MgSO₄ addition had little effect at the mill-relevant concentrationof 1 g/L, whereas the deliberately elevated concentration of 10 g/Lincreased lignin retention. Overall, the results demonstrate thatliquor chemistry influences the balance between lignin removal andretention during brownstock washing. Because conventional mill-scaleindicators such as chemical oxygen demand (COD), dissolved solidscarryover, and wash loss were not measured, the results are interpretedspecifically in terms of lignin redeposition and retention ratherthan overall washer performance.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"68453–68463"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Transcriptomic and Lipidomic Analysis of Amphotericin B-Susceptible and Resistant Clinical Isolates of Malassezia spp","authors":"Juan Camilo Galvis-Marín,&nbsp;Fredy Alexander Tabares-Villa,&nbsp;Augusto Zuluaga-Vélez,&nbsp;Paola Rojas-Estevez,&nbsp;Mónica P. Cala,&nbsp;Adriana Marcela Celis-Ramírez,&nbsp;Juan Carlos Sepúlveda-Arias","doi":"10.1021/acsomega.6c09344","DOIUrl":"https://doi.org/10.1021/acsomega.6c09344","url":null,"abstract":"<p><em>Malassezia</em> spp. are lipid-dependentskin commensalyeasts associated with superficial infections and fungemia. AmphotericinB remains a key treatment for systemic infections despite its toxicityand decreased susceptibility. This study characterized amphotericinB susceptibility profiles and identified transcriptomic and lipidomicsignatures of polyene resistance in clinical <em>Malassezia</em> isolates. Clinical isolates of <em>M</em>. <em>sympodialis</em> and <em>M</em>. <em>furfur</em>, and reference strains(CBS 7222 and CBS 1878), were evaluated using the modified M27-A3CLSI protocol for MICs and subinhibitory concentrations. One isolateper species was susceptible (MIC 0.5–2.0 μg/mL), whileanother was resistant (MIC ≥ 16 μg/mL). Transcriptomesgenerated by <em>Oxford Nanopore</em> sequencing were analyzedusing DESeq2 (|log2FC|&gt;2, <em>p</em> &lt; 0.05). Comparisonsbetween susceptible and resistant isolates revealed few DifferentiallyExpressed Genes (DEGs) that were not enriched for ergosterol biosynthesis.Interaction hubs indicated roles in nucleotide homeostasis, translation,chromatin regulation, and stress pathways. Lipidomic analysis by HPLC-QTOF-MSdetected approximately 200 lipid species, including 55 DifferentialLipid Molecules (DLMs) for <em>M</em>. <em>sympodialis</em> and 63 for <em>M</em>. <em>furfur</em>. Althoughglobal class abundances remained unchanged, resistant clinical isolatesshowed differences in the relative abundance of selected lipid species,with increased levels of long-chain fatty acids, diacylglycerols,and ceramides. Resistant <em>M</em>. <em>sympodialis</em> showed higher cholesterol levels. Integrated analysis separatedsusceptible and resistant groups, with DEGs–DLMs correlationshighlighting networks in membrane organization, stress tolerance,and metabolism. These findings suggest that amphotericin B resistancemay be associated with metabolic alterations and changes in membranelipid composition, supporting the use of multiomics markers for detectionand therapeutic strategies.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69193–69208"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering pH-Responsive trans-Ferulic Acid/κ-Carrageenan Beads for On-Demand Micronutrient Delivery in Plants","authors":"Hitasha Vithalani,&nbsp;Subhojit Ghosh,&nbsp;Harshil Dave,&nbsp;Kashish Agrawal,&nbsp;Mukesh Dhanka,&nbsp;Subramanian Sankaranarayanan","doi":"10.1021/acsomega.6c01871","DOIUrl":"https://doi.org/10.1021/acsomega.6c01871","url":null,"abstract":"<p>Micronutrient deficiencies in soils are a critical challengeinagriculture, particularly in acidic soil environments where nutrientavailability is strongly limited by fixation, leaching, and alteredmetal speciation. These constraints contribute to inefficient nutrientuptake and reduced crop yields. Conventional micronutrient supplementationmethods, such as bulk fertilizer application and foliar sprays, areoften inefficient, environmentally harmful, and unsustainable, underscoringthe need for smarter delivery systems tailored to soil pH conditions.In this study, we developed biodegradable, pH-responsive microbeadsfabricated from κ-carrageenan (κ-CG), sulphated polysaccharidederrived from algal biomass and trans-ferulic acid (TFA), a naturallyoccurring phenolic antioxidant with radical-scavenging activity. Theκ-carrageenan/TFA-based microbeads, designated as κ-TF-B,were synthesized through an eco-friendly process and optimized forsize, morphology, and stability. Characterization confirmed the successfulincorporation of the constituent functional groups, while swelling,degradation, and release studies demonstrated pH-responsive deliveryof essential micronutrients under acidic conditions (pH 4.0), mimickingacidic soil environments. The optimized κ-TF-B microbeads exhibitedenhanced swelling at acidic pH and sustained micronutrient releasethroughout the study period. In addition, the microbeads showed strongantioxidant activity, with a radical-scavenging efficiency of 93.4%,attributed to the presence of TFA. For plant applications, κ-TF-All,defined as the κ-TF-B microbead formulation loaded with allfour essential micronutrients (Mn²⁺, Zn²⁺, Cu²⁺, and Fe²⁺), was evaluated in soil and plant growthassays. κ-TF-All improved micronutrient availability and promotedplant growth, resulting in larger leaf area and a 3.3-fold increasein chlorophyll content in <em>Arabidopsis thaliana</em> compared with the negative control, without observable phytotoxiceffects. Collectively, these findings demonstrate the potential ofκ-TF-All microbeads as an eco-friendly and pH-responsive platformfor sustainable micronutrient delivery and improved plant growth undermicronutrient-limited conditions.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"68185–68197"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling Characteristics and Sensitivity Differences of Coal Porosity and Permeability with Stress in Different Wellfields of Fukang Mining Area","authors":"Bing Bai,&nbsp;Jienan Pan,&nbsp;Zhenzhi Wang,&nbsp;Yidong Li,&nbsp;Meng Li,&nbsp;Xuetian Du,&nbsp;Nannan Cheng","doi":"10.1021/acsomega.6c09182","DOIUrl":"https://doi.org/10.1021/acsomega.6c09182","url":null,"abstract":"<p>Quantifying how coal porosity and permeability vary witheffectivestress is essential for forecasting coalbed methane (CBM) deliverability,yet the coupled stress response of low-rank coals and its variationamong wellfields remain poorly constrained. Nine cores from threewellfields of the Fukang mining area─Kuangou (KG), Wudong (WD),and Donggou (DG)─were tested under stepwise hydrostatic loadingof 2.07–15.86 MPa while porosity and permeability were measuredsimultaneously. Both properties decrease exponentially with stress,but permeability declines far more strongly: at maximum loading, theporosity damage ratio ranges from 15.9% to 69.55%, whereas the permeabilitydamage ratio reaches 82.2–96.7%, and permeability sensitivityacross wellfields follows DG ≈ WD &gt; KG. A porosity–permeabilityratio (e = φ/k) and a relative sensitivity parameter (R) wereintroduced to quantify the storage–seepage mismatch, with erising continuously along the loading path. Fracture density, aperture,pore-throat connectivity, and mineral infilling jointly govern theheterogeneous response. The results provide quantitative criteriafor staged depressurization and fracturing design in low-rank CBMdevelopment.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69098–69116"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chirality as a Structural Degree of Freedom in Halide Perovskites","authors":"Suhas K. T.,&nbsp;Ranjani Viswanatha","doi":"10.1021/acsomega.6c10705","DOIUrl":"https://doi.org/10.1021/acsomega.6c10705","url":null,"abstract":"<p>Chirality in halide perovskites has traditionally beenassociatedwith the incorporation of intrinsically chiral molecular components.Here, we develop a broader perspective in which chirality can emergefrom the collective structural organization of an otherwise achiralinorganic lattice. We distinguish the structural origins of chirality,including molecular or ligand-mediated interactions, cooperative latticedistortions, surfaces and interfaces, and dynamic structural fluctuations,from the thermodynamic and structural parameters that control thesestates, including strain, dimensionality, confinement, temperature,pressure, and composition. Particular emphasis is placed on distinguishingstructural distortion and inversion symmetry breaking from genuinechirality, which requires the absence of all improper symmetry operationsand sufficient spatial correlation of the handed structural state.Two halide perovskite systems, Cs₂PbI₂Cl₂ nanoplatelets and CsPbBr₃ nanorods, illustratecomplementary examples in which structural perturbations are associatedwith pronounced chiroptical responses. We further outline experimentalcriteria for establishing structural chirality and discuss opportunitiesfor selecting, switching, and coupling handed structural states tooptical, electronic, spin, and magnetic functionalities.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"67974–67986"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Photophysical Properties of Electroactive Apocynin Derivatives as Potential Luminogens and Cellular Bioimaging Agents","authors":"Camila García-Aquite,&nbsp;Luis Illicachi,&nbsp;Joel José Montalvo Acosta,&nbsp;Alejandro Ortiz,&nbsp;Homero G. San-Juan-Vergara,&nbsp;Juan D. Rodríguez,&nbsp;Walter A. Martínez-De-La-Rosa,&nbsp;Daniel Insuasty,&nbsp;Alberto Insuasty","doi":"10.1021/acsomega.6c09229","DOIUrl":"https://doi.org/10.1021/acsomega.6c09229","url":null,"abstract":"<p>This study describesthe synthesis, photophysical characterization,and bioimaging properties of a series of chalcones derived from apocynin,functionalized with electroactive groups such as phenyldimethylamine(<strong>1</strong>), triphenylamine (<strong>2</strong>), tetraphenylethylene(<strong>3</strong>), and pyrene (<strong>4</strong>). These molecules featureddonor−π–acceptor–donor (D−π–A–D)type architectures designed to promote intramolecular charge transfer(ICT) processes and notable emissive properties. Compounds <strong>1</strong> and <strong>2</strong> exhibited a marked aggregation-caused quenching(ACQ) behavior in THF/water mixtures while compounds <strong>3</strong> and <strong>4</strong> showed a marked enhancement of emission uponaggregation (AIE), associated with the restriction of intramolecularmotions in the aggregated state. DFT/TD-DFT calculations and electrochemicalstudies revealed good electronic communications between the electroactiveunits. Cellular bioimaging studies performed on compounds <strong>1–4</strong> showed that only compounds <strong>1</strong>, <strong>2</strong>, and <strong>4</strong> are good fluorescent agents exhibiting notable cellularpermeability in HEp-2 carcinoma cells. Overall, these findings demonstratethat apocynin α,β-unsaturated systems constitute versatileluminescent platforms with tunable photophysical properties, suitablefor applications in cellular bioimaging and the development of remarkablefluorescent materials.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69127–69142"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Kinetic Relaxation Spectroscopy for Discrimination of Volatile Organic Mixtures via Finite-Size Surface Gratings","authors":"Narimane Hassaine,&nbsp;Luca Moretti,&nbsp;Riccardo Castagna,&nbsp;Davide Bizzaro,&nbsp;Oriano Francescangeli,&nbsp;Costanzo Di Perna,&nbsp;Andrea Di Donato,&nbsp;Daniele E. Lucchetta","doi":"10.1021/acsomega.6c08719","DOIUrl":"https://doi.org/10.1021/acsomega.6c08719","url":null,"abstract":"<p>Discrimination ofchemically similar volatile organiccompounds(VOCs) in gas mixtures remains a challenge for traditional opticalrefractive index sensors. In this work, we used a surface holographicgrating relaxation technique to monitor the transport kinetics ofVOCs. By analyzing the decay of the diffraction efficiency, we extracttwo independent relaxation time constants. These constants arise fromthe interplay between the local diffusion of the gas molecules throughthe material and the geometric aperture losses of the sensing surface(diffusion of molecules in air underneath the grating sensor). Theseparameters provide a unique kinetic fingerprint for individual species.Mapping these values in a 2D inverse-time state space reveals distinct,nonoverlapping clusters. Furthermore, in the presence of multicomponentgas molecules, by mapping the relaxation lifetimes of pure volatilesand their binary mixtures, we identify a predictable transition inthe state space. This approach enables the discrimination betweenindividual volatile compounds and mixtures (e.g., methanol-ethanol)and, in principle, allows their respective concentrations to be quantifiedand monitored over time with a high degree of sensitivity. This dual-parameterapproach uses the finite-size effects of the probe beam, traditionallyconsidered an experimental limitation, to enhance sensor selectivity,offering a robust framework for miniaturizing complex gas-phase analysis.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69186–69192"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Design and Synthesis of Hybrid PEGylated Metal Monopicolinate Cyclam Ligands for Biomedical Applications”","authors":"Fatima Aouidat,&nbsp;Zakaria Halime,&nbsp;Rosalba Moretta,&nbsp;Ilaria Rea,&nbsp;Stefania Filosa,&nbsp;Stella Donato,&nbsp;Rosarita Tatè,&nbsp;Luca de Stefano,&nbsp;Raphaël Tripier,&nbsp;Jolanda Spadavecchia","doi":"10.1021/acsomega.6c11010","DOIUrl":"https://doi.org/10.1021/acsomega.6c11010","url":null,"abstract":"","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69409–69409"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryogenically Deposited Plasmonic Au Nanoparticle Films for Photothermal Treatment of Lung Cancer Cells","authors":"Vagif Nevruzoglu,&nbsp;Murat Tomakin,&nbsp;Nazlıhan Aztopal,&nbsp;Melih Manir,&nbsp;Tugba Bayazıt,&nbsp;Fatma Kurul,&nbsp;Bilge Can,&nbsp;Arif E. Cetin","doi":"10.1021/acsomega.6c06589","DOIUrl":"https://doi.org/10.1021/acsomega.6c06589","url":null,"abstract":"<p>In this study, Au nanoparticle-coated glass substrateswere fabricatedby a cryogenic nonequilibrium deposition method at substrate temperaturesof 300 and 230 K for plasmonic photothermal therapy applications.Field-emission scanning electron microscopy analysis revealed thatthe Au films deposited at 300 and 230 K consisted of nanoparticleassemblies with size distributions of approximately 7–17 nmand 8–10 nm, respectively. The significantly narrower particlesize distribution observed at 230 K could be attributed to the emergenceof a morphology consistent with the proposed soliton-mediated nonequilibriumgrowth mechanism characteristic of cryogenic deposition conditions.Atomic force microscopy measurements demonstrated that the averagesurface roughness decreased from 5.27 nm for the 300 K sample to 1.55nm for the 230 K sample, confirming the formation of a more homogeneousnanoparticle architecture under cryogenic growth conditions. Opticalcharacterization showed that both Au nanoparticle films exhibitedsurface plasmon resonance near 600 nm. However, the cryogenicallyfabricated film produced at 230 K displayed a substantially strongerplasmonic response, which was attributed to its enhanced structuraluniformity and improved interparticle plasmonic coupling. To evaluatetheir photothermal performance, human lung adenocarcinoma A549 cellsand normal bronchial epithelial BEAS-2B cells were cultured on bare-glassand Au nanoparticle-coated substrates and subsequently exposed to∼600 nm irradiation using a 450 mW light source. Morphologicaland fluorescence-based analyses revealed pronounced photothermal-inducedcytotoxicity in A549 cells cultured on Au-coated surfaces, includingreduced cell density, disruption of cell–cell interactions,membrane damage, and loss of cellular organization. In contrast, BEAS-2Bcells largely preserved their morphology, adhesion behavior, and membraneintegrity under identical treatment conditions. The strongest cytotoxicresponse was observed for the Au nanoparticle films fabricated at230 K. These findings suggest that cryogenic nonequilibrium depositioncan improve nanoparticle uniformity and plasmonic performance, withthe 230 K Au nanoparticle films producing the most pronounced photothermalresponse under the investigated conditions. In the preliminary biologicalevaluation, A549 cells exhibited a greater photothermal response thanBEAS-2B cells under identical experimental conditions. These proof-of-conceptfindings highlight the potential of cryogenically fabricated plasmonicnanostructures for further investigation in photothermal biomedicalapplications.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"68565–68578"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Ethanol Degradation Using Geobacter metallireducens–Shewanella oneidensis Coculture Bioanodes in Single-Chamber Microbial Electrolysis Cells","authors":"Avinash Jukanti,&nbsp;Vamshi Krishna Bommagani,&nbsp;Abhishiktha Chiliveru,&nbsp;Alex Schechter,&nbsp;Rivka Cahan","doi":"10.1021/acsomega.6c10207","DOIUrl":"https://doi.org/10.1021/acsomega.6c10207","url":null,"abstract":"<p>Ethanol-rich effluents from biorefineries, distilleries,and fermentationindustries are abundant but remain difficult to valorize because alcoholoxidation rarely aligns with the extracellular electron-transfer (EET)capacity of single exoelectrogenic species. A coculture bioanode combines <em>Geobacter metallireducens</em> (GM), which carries high-fluxc-type cytochrome EET but modest alcohol dehydrogenase activity, with <em>Shewanella oneidensis</em> MR-1 (SO). The coculture wasoperated in single-chamber microbial electrolysis cells (MECs) for82 days. The MEC was enriched first with sodium acetate (SA, 1600mg L^–1) and then with the transitional feed (800mg L^–1 SA + 5 mM ethanol, followed by 5 to 20 mMethanol). The GM+SO coculture outperformed both monocultures in current,with the hierarchy GM+SO &gt; GM &gt; SO preserved across every substratephase. LSV current density reached 1.66 ± 0.27 A m^–2 in GM+SO under 5 mM ethanol, representing 2.7- and 8-fold enhancementsover GM and SO, respectively. GM+SO achieved the fastest degradationrate when ethanol was used as the carbon source, at 4.68 ± 0.55mM d^–1; it retained ethanol-removal capacity at20 mM when GM collapsed under substrate inhibition. End point 16SrRNA sequencing showed that <em>Geobacter</em> and <em>Shewanella</em> accounted for 45% and 20% of reads in the GM+SObiofilm. PICRUSt2 predicted greater potential for alcohol and aldehydedehydrogenases and c-type cytochrome functions in the coculture, consistentwith a hypothesized pathway in which both partners oxidize ethanolto acetate and <em>Geobacter</em> dominates electron deliveryto the anode. Thus, the benefit of coinoculation depends on substrateand loading, and substrate removal alone is a poor guide to partnerselection for ethanol-treating bioanodes.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"11 44","pages":"69348–69361"},"PeriodicalIF":5.2,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149375087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}