ACS Omega最新文献

{"title":"In Vitro and In Vivo Comparison of Random versus Site-Specific Conjugation of Bifunctional Chelating Agents to the CD33-Binding Antibody for Use in Alpha- and Beta-Radioimmunotherapy","authors":"Kevin J. H. Allen,&nbsp;Connor Frank,&nbsp;Rubin Jiao,&nbsp;Mackenzie E. Malo,&nbsp;Michele Bello,&nbsp;Laura De Nardo,&nbsp;Laura Meléndez-Alafort and Ekaterina Dadachova*,&nbsp;","doi":"10.1021/acsomega.4c0945010.1021/acsomega.4c09450","DOIUrl":"https://doi.org/10.1021/acsomega.4c09450https://doi.org/10.1021/acsomega.4c09450","url":null,"abstract":"<p >Radiometal chelator conjugation is a cornerstone of radioimmunotherapy (RIT). Continued interest in selective placement of chelators remains an active topic of discussion in the field. With several simple site-specific methods being recently reported, it was of interest to investigate the benefits and potential drawbacks of the site-specific method with a full comparison to a more typical random conjugation method that is currently utilized in clinical applications. In this study, the conjugation methods were evaluated side by side to determine the utility of both methods using commercially available random and site-specific conjugation reagents by performing antigen binding; radiolabeling with ⁶⁴Cu, ¹⁷⁷Lu, and ²²⁵Ac radioisotopes; antibody-conjugate stability, cytotoxicity, in vivo distribution, pharmacokinetics analyses, and dosimetry to gather a whole data set for preclinical investigation. Evaluation revealed that both methods performed similarly during most experiments with the site-specific method, resulting in higher binding capacity of the antibody conjugate via flow cytometry. Radiolabeling was not significantly different between two methods, while stability showed that the site-specifically conjugated antibody was somewhat more stable at 37 °C in human serum over 1 week. In vitro experiments demonstrated less cell killing with the random conjugation method, while in vivo experiments showed no statistical differences in tumor uptake between conjugation methods. Dosimetry calculations were performed using the acquired PET/CT data and showed that apart from the liver, there was no significant difference in radiation doses delivered by either antibody conjugate. These results demonstrate that both methods are viable for future work, while the site-specific method offers several potential advantages and, in some cases, improved efficacy.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"50000–50011 50000–50011"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c09450","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142850234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable Transparent Conductors Based on SnO2: Theoretical and Experimental Studies of Codoping","authors":"Wenjing Qian,&nbsp;Xianghui Feng,&nbsp;Yanxue Wang,&nbsp;Ahmet Nazligul,&nbsp;Yiwen Lu,&nbsp;Mingqing Wang*,&nbsp;Wei Wu* and Kwang Leong Choy*,&nbsp;","doi":"10.1021/acsomega.4c0786010.1021/acsomega.4c07860","DOIUrl":"https://doi.org/10.1021/acsomega.4c07860https://doi.org/10.1021/acsomega.4c07860","url":null,"abstract":"<p >Transparent conducting oxides (TCOs) are widely used in modern electronics because they have both high transmittance and good conductivity, which is beneficial for many applications such as light-emitting diodes. Tailoring electronic states and hence the conductive types by design is important for developing new materials with optimal properties for TCOs. SnO₂, with a wide band gap, low cost, no toxins, and high stability, is a promising host material for TCOs. Here, we performed a set of hybrid-exchange density functional theory calculations on the two-element and three-element codoped SnO₂ by using Sr, Ta, Al, Ga, V, and Nb, which were then validated by the relevant experimental works on SnO₂. As predicted by the first-principles calculations, the controllability of the electronic states to be n- or p-type can be demonstrated experimentally by varying the relative doping concentration between donors (Ta/Nb) and acceptors (Al/Ga). One of the main advantages for these codoping methods is that the charge neutrality problem caused by the dopant can be circumvented. The thin films fabricated showed a low sheet resistance (down to ∼450 Ω<b>/□</b>) and a high optical transparency (above 80%). The combination of our calculations and experimental material fabrication and characterizations has shown a great potential for codoping SnO₂ for (i) the efficient processing of the integrated circuit composed of both p-type and n-type transistors (using the same target precursors during the deposition) and (ii) a good lattice matching for p–n junctions. Most importantly, our calculations, supported by the experimental works, point to a promising route to accelerate the discovery process for the alternative cost-effective and high-performance indium-free TCOs using computational material design.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49674–49682 49674–49682"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c07860","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142850050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soyasaponin Bb/Gelatin-Methacryloyl Hydrogel for Cartilage Inflammation Inhibition.","authors":"Yuhan Jiang, Tenghai Li, Bingzhang Liu, Yufeng Tian, Yixin Wang, Tian Li, Duo Zhang","doi":"10.1021/acsomega.4c07489","DOIUrl":"https://doi.org/10.1021/acsomega.4c07489","url":null,"abstract":"<p><p>The main causes of failure for cartilage tissue engineering implants are tissue integration, inflammation, and infection. The development of biomaterials with antiforeign body response (FBR) is of particular importance. Herein, we developed a hydrogel loaded with anti-inflammatory drugs to reduce the inflammatory response that follows implantation. The human chondrocytes were used for in vitro study, and cell-laden hydrogel samples were implanted with the backs of rabbits for in vivo study. Soyasaponin Bb (SsBb) as a traditional Chinese medicine could significantly (<i>P</i> < 0.05) downregulate the expression levels of inflammation-related markers including iNOS, COX-2, and IL-6 in chondrocytes induced by IL-1β through the NF-κB signaling pathway. The in vitro experiments demonstrated that a gelatin-methacryloyl (GelMA) hydrogel loaded with SsBb (SsBb/GelMA) could similarly reduce the gene and protein expression levels of inflammation-related markers (iNOS, COX-2, and IL-6). The in vivo anti-inflammatory effects of the SsBb/GelMA hydrogels were assessed by immunohistochemical staining. The results demonstrated that SsBb/GelMA hydrogels inhibited the inflammatory response and downregulated the expression of the inflammatory cytokine IL-6. Therefore, SsBb/GelMA hydrogels are promising candidates for promoting anti-inflammation and cartilage tissue regeneration of implant surfaces.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49597-49608"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing Local Structure of Angiotensin Receptor-Neprilysin Inhibitor (S086) Drug Cocrystal by Linear and Nonlinear Infrared Spectroscopies","authors":"Wenjie Xu,&nbsp;Haiyan Xu,&nbsp;Jie Yan,&nbsp;Song Li,&nbsp;Pengyun Yu,&nbsp;Juan Zhao,&nbsp;Fan Yang and Jianping Wang*,&nbsp;","doi":"10.1021/acsomega.4c0788710.1021/acsomega.4c07887","DOIUrl":"https://doi.org/10.1021/acsomega.4c07887https://doi.org/10.1021/acsomega.4c07887","url":null,"abstract":"<p >Structurally knowing the active sites of a drug is important for understanding its therapeutic functions. S086 is a novel angiotensin receptor-neprilysin inhibitor that consists of the molecular moieties of EXP3174 (the active metabolite of the angiotensin receptor blocker losartan) and sacubitril (a neprilysin inhibitor prodrug) in a 1:1 molar ratio. There are two forms of cocrystals of S086, namely, ξ-crystal and α-crystal, which were formed both via intermolecular coordination bonding to calcium ions, with the aid of internal water. The binding state of multiple carboxyl anions (COO^–) to Ca²⁺ of EXP3174 and sacubitril was examined in this study using infrared (IR) absorption spectroscopy, in which the asymmetric stretching (<i>as</i>) and symmetric stretching (<i>ss</i>) modes of the COO^– groups were used as IR probes. Ultrafast two-dimensional (2D) IR spectroscopy was utilized for spectrally assigning the origin of multiple COO^– groups by the presence or absence of interchromophore vibrational coupling. Key structural variation between the two crystal forms was found: in the unit cell of ξ-crystal, the ratio of “bridging” and “bidentate” types of COO^– binding to Ca²⁺ for four EXP3174 molecules is 2:2, while the ratio is predicted to be 3:1 in the case of α-crystal. However, in both crystals, four sacubitril molecules are believed to similarly form a “trident” type of COO^– binding to Ca²⁺. Our study demonstrates that linear and nonlinear IR spectroscopies can be used to characterize local crystal structures of drugs and reveal subtle difference between similar crystal structures.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49683–49691 49683–49691"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c07887","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142842082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Step Deposition of Chalcopyrite (CuFeS2) Thin Films","authors":"Valmar da Silva Severiano Sobrinho,&nbsp;Thercio Henrique de Carvalho Costa*,&nbsp;Michelle Cequeira Feitor,&nbsp;Maxwell Santana Libório,&nbsp;Rômulo Ribeiro Magalhães de Sousa,&nbsp;Álvaro Albueno da Silva Linhares,&nbsp;Pâmala Samara Vieira,&nbsp;Luciano Lucas Fernandes Lima,&nbsp;Cleânio da Luz Lima and Edcleide Maria Araújo,&nbsp;","doi":"10.1021/acsomega.4c0864710.1021/acsomega.4c08647","DOIUrl":"https://doi.org/10.1021/acsomega.4c08647https://doi.org/10.1021/acsomega.4c08647","url":null,"abstract":"<p >Thin films of chalcopyrite, CuFeS₂, are promising candidates for use as absorber layers in photovoltaic cells due to their low band gap and high absorbance. These films are typically deposited in two or three steps, always involving an annealing process. In this work, the CuFeS₂ film was deposited on a glass substrate in a single deposition step using the cathodic cylindrical plasma deposition (CCyPD) technique. The film samples deposited were analyzed by X-ray diffraction (XRD) and Raman spectroscopy, the film thickness was measured using the optical method, and FEG-SEM analyzed the surface structural morphology. The results showed a strong dependence on the deposition temperature for phase formation, with chalcopyrite being obtained for films deposited at 600 °C. At this temperature, a uniformly distributed film with uniform grain sizes was obtained, and the experimentally obtained band gap values of the films were consistent with the theoretical values reported in the literature, demonstrating the technique’s effectiveness and precision in producing high-quality films.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49849–49856 49849–49856"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142842573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Aqueous Stability of Anionic Surfactants in High Salinity and Temperature Conditions with SiO₂ Nanoparticles.","authors":"Mohammed H Alyousef, Muhammad Shahzad Kamal, Mobeen Murtaza, Syed Muhammad Shakil Hussain, Arshad Raza, Shirish Patil, Mohamed Mahmoud","doi":"10.1021/acsomega.4c08484","DOIUrl":"https://doi.org/10.1021/acsomega.4c08484","url":null,"abstract":"<p><p>In chemical-enhanced oil recovery (cEOR), surfactants are widely used but face significant stability challenges in high-salinity brine, where they often degrade or precipitate. Existing methods, such as adding cosurfactants, offer limited compatibility with anionic surfactants and raise economic concerns, creating a need for more robust solutions. This study introduces a novel approach to enhance the stability of anionic surfactants in extreme salinity conditions by incorporating silicon dioxide (SiO₂) nanoparticles (NPs). Our optimized formulation effectively prevents surfactant precipitation and NP aggregation, demonstrating stability in brine with salinity as high as 57,000 ppm and temperatures up to 70 °C, thus addressing the salt tolerance issues seen with conventional anionic surfactants like sodium dodecyl sulfate (SDS). To validate our formulation, we employed multiple experimental techniques, including turbidity, ζ-potential (ZP), and hydrodynamic diameter (HDD) measurements, which confirmed the efficacy of our approach. Results indicated that an optimal SiO₂ NP concentration (0.01 wt %) significantly enhanced SDS stability, with no observed aggregation or precipitation over 7 days. High absolute ZP values (>25 mV), a small HDD (∼37 nm), and a consistent turbidity profile underscored the stability and dispersion of the formulation. This nanoparticle-based method offers a cost-effective and sustainable solution for cEOR, providing enhanced surfactant stability and improved NP dispersibility under high-salinity and high-temperature conditions, representing a valuable advancement in chemical-enhanced oil recovery technology.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49804-49815"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Step Deposition of Chalcopyrite (CuFeS₂) Thin Films.","authors":"Valmar da Silva Severiano Sobrinho, Thercio Henrique de Carvalho Costa, Michelle Cequeira Feitor, Maxwell Santana Libório, Rômulo Ribeiro Magalhães de Sousa, Álvaro Albueno da Silva Linhares, Pâmala Samara Vieira, Luciano Lucas Fernandes Lima, Cleânio da Luz Lima, Edcleide Maria Araújo","doi":"10.1021/acsomega.4c08647","DOIUrl":"https://doi.org/10.1021/acsomega.4c08647","url":null,"abstract":"<p><p>Thin films of chalcopyrite, CuFeS₂, are promising candidates for use as absorber layers in photovoltaic cells due to their low band gap and high absorbance. These films are typically deposited in two or three steps, always involving an annealing process. In this work, the CuFeS₂ film was deposited on a glass substrate in a single deposition step using the cathodic cylindrical plasma deposition (CCyPD) technique. The film samples deposited were analyzed by X-ray diffraction (XRD) and Raman spectroscopy, the film thickness was measured using the optical method, and FEG-SEM analyzed the surface structural morphology. The results showed a strong dependence on the deposition temperature for phase formation, with chalcopyrite being obtained for films deposited at 600 °C. At this temperature, a uniformly distributed film with uniform grain sizes was obtained, and the experimentally obtained band gap values of the films were consistent with the theoretical values reported in the literature, demonstrating the technique's effectiveness and precision in producing high-quality films.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49849-49856"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooling Performance of TiO2-Based Radiative Cooling Coating in Tropical Conditions","authors":"Bhrigu Rishi Mishra,&nbsp;Sreerag Sundaram and Karthik Sasihithlu*,&nbsp;","doi":"10.1021/acsomega.4c0722310.1021/acsomega.4c07223","DOIUrl":"https://doi.org/10.1021/acsomega.4c07223https://doi.org/10.1021/acsomega.4c07223","url":null,"abstract":"<p >The cooling power of radiative cooling (RC) coatings depends not only on the radiative properties of the coating but also on environmental variables. In tropical environments, the cooling performance of RC coatings deteriorates due to high humidity and high solar radiation. Previous studies focused on developing high solar-reflective coatings to achieve subambient cooling in tropical environments. However, these coatings have not demonstrated the ability to be used at a large scale, mainly due to their high cost or less durability. Herein, we test an RC paint coating composed of TiO₂ and polydimethylsiloxane (PDMS) in three different cities with high and moderate humidity levels. Though a significant reduction in the internal temperature of an RC paint-coated aluminum (Al) box is observed, compared to an uncoated Al box, in both high and moderate humidity environments, subambient cooling is not achieved. A comprehensive analysis is conducted to clarify the reasons behind the inability to attain subambient cooling.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49494–49502 49494–49502"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c07223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142842083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic Nanocarriers for pH/GSH/NIR Triple-Responsive Drug Release and Synergistic Therapy in Tumor Cells","authors":"Di Zhang,&nbsp;Wanyu Wei,&nbsp;Tianxiang Xie,&nbsp;Xue Zhou,&nbsp;Xu He,&nbsp;Jie Qiao,&nbsp;Rui Guo*,&nbsp;Gang Jin* and Ningbo Li*,&nbsp;","doi":"10.1021/acsomega.4c0826710.1021/acsomega.4c08267","DOIUrl":"https://doi.org/10.1021/acsomega.4c08267https://doi.org/10.1021/acsomega.4c08267","url":null,"abstract":"<p >In this study, the mesoporous Fe₃O₄ nanodrug carriers containing disulfide bonds (CHO-SMNPs) were successfully synthesized and characterized. Doxorubicin (DOX) was loaded onto the CHO-SMNPs as a model drug and gatekeeper through the formation of imine bonds with the aldehyde groups on the surface of the mesoporous materials. This drug carrier demonstrates effective drug release triggered by pH, glutathione (GSH), and near-infrared (NIR) light, along with satisfactory photothermal conversion efficiency under NIR irradiation at 808 nm. Furthermore, CHO-SMNPs exhibit excellent blood compatibility and biodegradability. They also show good biocompatibility and efficient cellular uptake in HeLa and MCF-7 cancer cells. Most importantly, the CHO-SMNPs/DOX has shown significant effectiveness in killing both HeLa and MCF-7 cancer cells. Consequently, CHO-SMNPs/DOX presents substantial potential as a magnetic-targeted, pH/GSH/NIR triple-triggered drug delivery system for synergistic chemo-photothermal therapy in tumor treatment.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49749–49758 49749–49758"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142842677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic Dissolution Products of Lithium-, Strontium-, and Boron-Substituted Silicate Glasses Influence the Viability and Proliferation of Adipose Stromal Cells, Fibroblasts, Urothelial and Endothelial Cells","authors":"Inari Lyyra*,&nbsp;Mari Isomäki,&nbsp;Heini Huhtala,&nbsp;Minna Kellomäki,&nbsp;Susanna Miettinen,&nbsp;Jonathan Massera and Reetta Sartoneva,&nbsp;","doi":"10.1021/acsomega.4c0658710.1021/acsomega.4c06587","DOIUrl":"https://doi.org/10.1021/acsomega.4c06587https://doi.org/10.1021/acsomega.4c06587","url":null,"abstract":"<p >While bioactive glasses (BaGs) have been studied mainly for bone applications, studies have also shown their potential for soft tissue engineering. Incorporating therapeutic ions, such as lithium (Li⁺), strontium (Sr²⁺), and boron (B³⁺) into the BaGs, has been found to promote angiogenesis and wound healing. However, a systematic study on the impact of Li⁺, Sr²⁺, B³⁺, and the other ions in the BaGs, has not been conducted on a wide range of cells. Although the interactions between the BaGs and cells have been studied, it is difficult to compare the results between studies and conclude the impact of BaGs between cell types due to the variability of culture conditions, cells, and materials. We aim to evaluate the dissolution behavior of Li-, Sr-, and B-substituted BaGs and the effects of their ionic dissolution products on the viability, proliferation, and morphology of multiple cell types: human adipose stromal cells (hASCs), human lung fibroblasts (cell line WI-38), human urothelial cells (hUCs), and human umbilical vein endothelial cells (HUVECs). In the dissolution study, the B-substituted glasses induced a higher increase in pH and released more ions than the silicate glasses. The undiluted BaG extracts supported the viability and proliferation of all the other cell types except the hUCs. Diluting the BaG extracts to 1:10 restored the viability of hUCs but induced distinctive morphological changes. Diluting the extracts more (1:100) almost fully restored the hUC morphology. To conclude, the ionic dissolution products of Li-, Sr-, and B-substituted BaGs seem beneficial for hASCs, WI-38, hUCs, and HUVECs, but attention must be paid to the ion concentrations.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"9 50","pages":"49348–49367 49348–49367"},"PeriodicalIF":3.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c06587","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142850321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}