ACS Omega最新文献

{"title":"Phlorizin Prolongs the Lifespan of <i>Caenorhabditis elegans</i> by Insulin and SIR-2.1 Regulation.","authors":"Xiaohan Zhang, Hao Wang","doi":"10.1021/acsomega.4c08725","DOIUrl":"10.1021/acsomega.4c08725","url":null,"abstract":"<p><p>Phlorizin has significant antioxidant properties and was studied using <i>Caenorhabditis elegans</i> to explore potential antiaging mechanisms. Results showed that phlorizin mitigated the harmful effects of high temperatures and hydrogen peroxide, reduced oxidative stress, increased antioxidant enzyme activity, and reduced malondialdehyde levels. Network pharmacological analysis reveals that the AKT1, INSR, and SOD2 signaling pathways play a key role in the antiaging effects of phlorizin. Its action is mediated by insulin and SIR-2.1, influencing DAF-16, SKN-1, and downstream genes in the antiaging effects. This implicates phlorizin as a promising functional food additive targeting the DAF-16 and SOD-3 axes for antiaging.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"11922-11934"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Greener and Whiter Analytical Procedure for Theobromine and Caffeine Determination in Tea Using Dimethyl Carbonate as an Extraction Solvent and Mobile Phase Constituent in Reversed-Phase Liquid Chromatography.","authors":"Oktawia Kalisz, Martina Catani, Szymon Bocian","doi":"10.1021/acsomega.4c11625","DOIUrl":"10.1021/acsomega.4c11625","url":null,"abstract":"<p><p>For the first time, dimethyl carbonate was applied as the sole organic solvent, both as an eluent in solid-phase extraction (SPE) and for the simultaneous determination of theobromine and caffeine in tea extracts using reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC). The proposed procedure has been validated and complies with the requirements of green analytical chemistry (GAC). Solid-phase extraction was used to purify the samples, achieving high recovery rates (97-101%) and precision (RSD < 2%). The method showed satisfactory sensitivity (achieving limit of quantitation values of 0.2 ng for theobromine and 0.35 ng for caffeine), although it was poorer compared to the developed conventional HPLC method using methanol, where the values reached 0.045 and 0.05 ng, respectively. The low toxicity and minimal solvent consumption of DMC contributed to a much more beneficial environmental profile of the developed method, as confirmed by AGREEprep, AGREE, and ChlorTox evaluations. Moreover, by switching from conventional HPLC to UHPLC, the remaining principles of GAC were further incorporated by reducing solvent consumption, analysis time, and energy requirements. In addition, the RGB tool highlighted the higher environmental performance of the method using DMC while not compromising analytical quality or economic practicality. The robustness and environmental benefits make this method a viable alternative for the routine analysis of tea ingredients, supporting sustainable analytical practices.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"12432-12440"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Surface Roughness and Strain Durability of Eyeglasses Frames by the 3D Printing Technology","authors":"Burak Malik Kaya*,&nbsp; and ,&nbsp;Celal Asici,&nbsp;","doi":"10.1021/acsomega.4c1059210.1021/acsomega.4c10592","DOIUrl":"https://doi.org/10.1021/acsomega.4c10592https://doi.org/10.1021/acsomega.4c10592","url":null,"abstract":"<p >Three-dimensional (3D) printer technology has developed rapidly in recent years and therefore has become the focus of attention in many areas. It has begun to be widely used in many areas in industry, medicine, biomedical, engineering, basic sciences, etc. Among these areas, the optician sector has also widely used 3D technology. Offering personalized eyeglass frame design, freedom of color, shape, and size in frames, 3D technology offers many advantages and conveniences for users and manufacturers. In this project, a 3D printer with high precision and consistency was developed, and eyeglass frames were designed and produced using acrylonitrile butadiene styrene (ABS) and polyethylene terephthalate glycol (PETG) filament types, different printing temperatures, and layer thicknesses. The surface roughness and the durability of the frames were analyzed by using an optical microscope and performing bending tests, respectively. It was observed that the lowest roughness occurred in the ABS-printed frame with 0.20 mm layer thickness at 240 °C temperature, and the highest durability of 54.7 mε obtained with the ABS-printed frames fabricated with 0.20 mm layer thickness at 235 °C temperature. Average roughness (<i>R</i>_a), root-mean-square roughness (<i>R</i>_q), and maximum height of profile (<i>R</i>_<i>z</i>) parameters were obtained to analyze surface roughness with respect to temperature change for fabricated frames using ABS and PETG filaments. Thus, the study proves that the production and optimization of customized eyeglass frames can be used not only for commercial and educational purposes in optical stores and optician programs at universities but also in industry, engineering, and daily life purposes.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"12214–12223 12214–12223"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c10592","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Water-Soluble Cu(II) Complex-Immobilized Electrospun Hydrophobic Polycaprolactone Nanofiber Composites for Highly Controlled and Long-Term Release","authors":"Maira Khalid,&nbsp;Muhammad Adnan,&nbsp;Muhammad Farooq,&nbsp;Yabuta Yoshinori,&nbsp;Jeongjin Park,&nbsp;Azeem Ullah,&nbsp;Gopiraman Mayakrishnan* and Ick Soo Kim*,&nbsp;","doi":"10.1021/acsomega.4c0930510.1021/acsomega.4c09305","DOIUrl":"https://doi.org/10.1021/acsomega.4c09305https://doi.org/10.1021/acsomega.4c09305","url":null,"abstract":"<p >Water-soluble Cu complexes offer diverse applications in the biomedical field as Cu is an essential trace element for many physiological functions, including the wound healing process. Controlled delivery of such bioactive Cu complexes to the target system is a promising approach in biomedical applications. Herein, water-soluble Cu(II)–Schiff base complex-incorporated PCL nanofiber composites (PCL@C–1%, PCL@C–3%, and PCL@C–5%) were fabricated by the electrospinning process using a green solvent, acetic acid. Physicochemical properties of the resultant composite nanofibers were investigated by FE-SEM, EDS, TEM, UV–vis, FT–IR, XRD, TGA, BET, and XPS analyses. The successful incorporation of the Cu(II) complex into the PCL nanofiber was confirmed. Water contact angle (WCA) values revealed the hydrophobic nature of the PCL-composite nanofibers, which is also quite beneficial in the wound-healing process as it can create a hydrophobic barrier to prevent extra fluid absorption. To our delight, the release behavior of Cu complexes from the composite nanofibers was found to be gradual, highly controlled, and long-term release (up to 40 days). In addition, the resultant PCL composites demonstrated excellent antibacterial activity against both Gram-positive and Gram-negative bacteria. Overall, these findings provide significant insights into these Cu complex-incorporated PCL nanofiber membranes as potential antibacterial and long-term wound dressings.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 13","pages":"12961–12971 12961–12971"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c09305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MoTe2 Polymorphs: A DFT Approach to Structural, Electronic, Mechanical and Vibrational Properties","authors":"Lathifa Banu S,&nbsp;Kanimozhi Balakrishnan,&nbsp;Vasu Veerapandy,&nbsp;Nalini Vajeeston and Ponniah Vajeeston*,&nbsp;","doi":"10.1021/acsomega.5c0022610.1021/acsomega.5c00226","DOIUrl":"https://doi.org/10.1021/acsomega.5c00226https://doi.org/10.1021/acsomega.5c00226","url":null,"abstract":"<p >Molybdenum ditelluride (MoTe₂), a key member of the transition metal dichalcogenides (TMDCs) family, holds significant potential for applications in electronics, energy storage, and catalysis. Despite its importance, the range of MoTe₂ structural forms that has been explored is still limited. The primary aim of this research is to identify new stable MoTe₂ polymorphs that may exist under zero-temperature and zero-pressure conditions. This study offers an in-depth analysis of 11 different structural variations (polymorphs) of MoTe₂ using advanced computational methods based on density functional theory (DFT). By employing the Heyd-Scuseria-Ernzerhof (HSE06) hybrid functional, accurate calculations of electronic properties, such as band structure, are achieved. Bonding analysis, including charge density and electron localization, reveals consistent covalent interactions across the hexagonal and trigonal forms of MoTe₂. The study also assesses the mechanical stability of these polymorphs using elastic constants, identifying both stable and metastable forms. Additionally, phonon and thermal properties, including heat capacity and entropy, are calculated for all dynamically stable polymorphs. Raman and infrared spectra provide insights into their distinct vibrational modes. These findings help distinguish structural attributes relevant to layer-specific applications. This comprehensive investigation of MoTe₂ polymorphs uncovers new stable structures and provides crucial insights for their potential use in technological applications.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 13","pages":"13515–13528 13515–13528"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.5c00226","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinomenine Inhibits TOPK To Ameliorate Psoriasiform Dermatitis","authors":"Hui Lu*,&nbsp;Fanfan Zeng,&nbsp;Hongjian Gong,&nbsp;Juan Du,&nbsp;Wenqi Gao,&nbsp;Jia Xu,&nbsp;Xiaonan Cai,&nbsp;Yuan Yang* and Han Xiao*,&nbsp;","doi":"10.1021/acsomega.5c0033610.1021/acsomega.5c00336","DOIUrl":"https://doi.org/10.1021/acsomega.5c00336https://doi.org/10.1021/acsomega.5c00336","url":null,"abstract":"<p >Psoriasis is an inflammatory skin disease. Sinomenine is an alkaloid extracted from the rhizome of <i>Sinomenium acutum</i>. Sinomenine plays an important role in inhibiting skin inflammation. However, the mechanism and targets of sinomenine in psoriasis remain unclear. In this study, we found that sinomenine bound and inhibited the TOPK activity <i>in vitro</i>. Moreover, sinomenine inhibited TOPK activation <i>ex</i> and <i>in vivo</i>. Furthermore, sinomenine ameliorated the psoriasiform phenotype <i>in vitro</i> and <i>in vivo</i>. In this study, we determined that TOPK is a potential target of sinomenine to alleviate psoriatic skin inflammation and to provide new processes for clinical treatment.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 13","pages":"13537–13547 13537–13547"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.5c00336","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity Analysis of Reservoir Characteristics and Flue Gas Composition for Enhanced Oil Recovery in Heterogeneous Reservoir.","authors":"Mehdi Nassabeh, Zhenjiang You, Alireza Keshavarz, Stefan Iglauer","doi":"10.1021/acsomega.4c09775","DOIUrl":"https://doi.org/10.1021/acsomega.4c09775","url":null,"abstract":"<p><p>The success of enhanced oil recovery depends on optimizing various reservoir factors. While CO₂ and flue gas injections are common methods, they encounter challenges that require a deep understanding of reservoir characteristics and thorough analysis of pertinent parameters for effective mitigation. This study aimed to explore the synergistic relationship between flue gas compositions, reservoir characteristics, and injection rates for optimal oil recovery. Unlike prior studies that primarily examined the isolated effects of CO₂ or flue gas, this research uniquely investigates the combined impact of real-world flue gas compositions from industrial sources under heterogeneous reservoir conditions. Through extensive sensitivity analysis and simulated optimization, the study analyzed the effects of different flue gas compositions on oil recovery. The results showed that flue gases with higher concentrations of CO₂ and O₂ exhibited higher recovery factor, while the presence of water vapor had a negative impact. CO₂ injection yielded the highest recovery factor overall. The behavior of flue gases closely resembled the actual field reservoir pressure. The analysis highlighted the significant influence of reservoir temperature, porosity, permeability, and homogeneity on oil recovery and reservoir pressure. Higher temperatures showed a positive correlation with increased oil recovery for CO₂ injection, while flue gas injection demonstrated a more pronounced improvement. Additionally, decreasing porosity had a significant positive effect on oil recovery, with CO₂ injection achieving the highest recovery factor under conditions of half the initial porosity. Permeability variations had minimal impact on oil recovery and reservoir pressure, particularly in heterogeneous reservoirs with high porosity and permeability. Furthermore, improving reservoir homogeneity by reducing deviation in permeability range and porosity positively impacted oil recovery and reservoir pressure, with flue gases benefiting more from this improvement. In terms of injection rates, CO₂ injection proved more effective in maintaining reservoir pressure compared to flue gas injection, but flue gas injections demonstrated greater sensitivity to increased injection rates. Notably, flue gas 1 exhibited superior efficiency in improving oil recovery with increasing injection rates compared to other flue gases.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"12135-12151"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Surface Roughness and Strain Durability of Eyeglasses Frames by the 3D Printing Technology.","authors":"Burak Malik Kaya, Celal Asici","doi":"10.1021/acsomega.4c10592","DOIUrl":"10.1021/acsomega.4c10592","url":null,"abstract":"<p><p>Three-dimensional (3D) printer technology has developed rapidly in recent years and therefore has become the focus of attention in many areas. It has begun to be widely used in many areas in industry, medicine, biomedical, engineering, basic sciences, etc. Among these areas, the optician sector has also widely used 3D technology. Offering personalized eyeglass frame design, freedom of color, shape, and size in frames, 3D technology offers many advantages and conveniences for users and manufacturers. In this project, a 3D printer with high precision and consistency was developed, and eyeglass frames were designed and produced using acrylonitrile butadiene styrene (ABS) and polyethylene terephthalate glycol (PETG) filament types, different printing temperatures, and layer thicknesses. The surface roughness and the durability of the frames were analyzed by using an optical microscope and performing bending tests, respectively. It was observed that the lowest roughness occurred in the ABS-printed frame with 0.20 mm layer thickness at 240 °C temperature, and the highest durability of 54.7 mε obtained with the ABS-printed frames fabricated with 0.20 mm layer thickness at 235 °C temperature. Average roughness (<i>R</i> _a), root-mean-square roughness (<i>R</i> _q), and maximum height of profile (<i>R</i> _<i>z</i> ) parameters were obtained to analyze surface roughness with respect to temperature change for fabricated frames using ABS and PETG filaments. Thus, the study proves that the production and optimization of customized eyeglass frames can be used not only for commercial and educational purposes in optical stores and optician programs at universities but also in industry, engineering, and daily life purposes.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"12214-12223"},"PeriodicalIF":3.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Apparent Permeability Model for Shale Matrix Derived under the Framework of the Energy Conservation Law","authors":"Shuai Chen*,&nbsp;Xulin Peng and Haoran Xu,&nbsp;","doi":"10.1021/acsomega.4c0835510.1021/acsomega.4c08355","DOIUrl":"https://doi.org/10.1021/acsomega.4c08355https://doi.org/10.1021/acsomega.4c08355","url":null,"abstract":"<p >With the rapid development of fracturing technology, unconventional oil and gas resources, such as shale oil and gas, are gradually becoming mainstream energy consumption. However, owing to the complex microstructure in shale reservoirs, it is still a challenge to accurately describe the gas transport characteristics in shale and predict the changes in its permeability. Gas transport in the shale matrix does not always fall into the continuum hypothesis of the Navier–Stokes equation; namely, when the Knudsen number increases, the proportion of the continuum flow is smaller. The gas extraction process is a nonlinear process under the alternate influence of different transport mechanisms and different microscale effects. In this work, the capillary tube space is divided into three different flow zones, and under the law of energy conservation, this study first establishes a bulk gas transport model of shale, which couples continuum flow and Knudsen diffusion. Also, based on the bulk gas transport model, an apparent permeability model of shale is developed by further considering the influence of surface diffusion, gas adsorption, and effective stress. In the apparent permeability model, this paper develops a slip velocity model, which is composed of gas coverage degree as a weight coefficient coupling both Knudsen diffusion velocity and surface diffusion velocity. It is found that the results calculated by the model fit well with the real observation. Moreover, compared with other apparent permeability models, the accuracy of the proposed model improves.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"11861–11872 11861–11872"},"PeriodicalIF":3.7,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of Research in Titanium-Based Lithium-Ion Sieves for the Extraction of Lithium from Salt Lake Brine","authors":"Yunyun Gao*,&nbsp;Yanxia Sun,&nbsp;Li Chen,&nbsp;Yao Tan,&nbsp;Rui Fan,&nbsp;Chunxi Hai and Hongjun Zhang,&nbsp;","doi":"10.1021/acsomega.4c0888610.1021/acsomega.4c08886","DOIUrl":"https://doi.org/10.1021/acsomega.4c08886https://doi.org/10.1021/acsomega.4c08886","url":null,"abstract":"<p >The lithium resource is a crucial national strategic asset, and China’s salt lakes are abundant in lithium. Efficient extraction of lithium from these salt lakes holds immense significance. Among the various methods available for the extraction of lithium from salt lakes, the adsorption method stands out due to its high selectivity, economic viability, and advanced process maturity. The key lies in developing high-performance adsorption materials, with lithium titanium oxide (LTO)-type lithium-ion sieves (LIS) being considered promising candidates. Therefore, we have concentrated on the structure and adsorption mechanism of titanium-based ion sieves and the preparation methods of different titanium oxide precursors in this review. Moreover, two modification methods, i.e., ion doping and surface coating, are discussed. Lastly, the insufficiency of current research is proposed, and the optimization and application of titanium-based LIS are prospected.</p>","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 12","pages":"11643–11657 11643–11657"},"PeriodicalIF":3.7,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08886","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}