Theoretical and Applied Genetics最新文献

{"title":"The role of N⁶-methyladenosine (m⁶A) modification in plants.","authors":"Cui-Xian Qin, Ao-Mei Li, Zhong-Liang Chen, Fen Liao, Miao Wang, You-Qiang Pan, Li Zhou, Bao-Qing Zhang, Jian-Hui Zhou, Dong-Liang Huang","doi":"10.1007/s00122-025-05042-3","DOIUrl":"https://doi.org/10.1007/s00122-025-05042-3","url":null,"abstract":"<p><p>N⁶-methyladenosine (m⁶A) modification is the most prevalent chemical modification identified in eukaryotic mRNA molecules, playing a pivotal role in plant growth, development, and responses to both biotic and abiotic stresses. m⁶A modification is co-regulated by methyltransferases and demethylases, with recognition proteins specifically binding to m⁶A modification sites to carry out more precise regulatory functions. This paper provides an overview of the discovery of m⁶A, its distribution patterns in plants, analyzes the molecular mechanisms underlying m⁶A modification, and compares the advantages and disadvantages of various detection techniques for m⁶A. Furthermore, we offer an extensive analysis of the roles of m⁶A modification in regulating plant growth and development, as well as its role in stress responses. Finally, we discuss the current challenges and propose perspective for future research. This work serves as a valuable reference for further research on plant m⁶A.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"258"},"PeriodicalIF":4.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The critical role of OsYTH10 in promoting early flowering of rice under long sunlight.","authors":"Jun Yang, Zhihao Chen, Peng Wang, Mvuyeni Nyasulu, Qin Cheng, Xiaopeng He, Jie Xu, Junru Fu, Dahu Zhou, Linjuan Ouyang, Haohua He, Jianmin Bian","doi":"10.1007/s00122-025-05041-4","DOIUrl":"https://doi.org/10.1007/s00122-025-05041-4","url":null,"abstract":"<p><p>RNA m6A modification plays a crucial role in plant growth and crop yield. Proteins that can recognize m6A modifications, known as m6A reader proteins, are essential for the regulatory functions of m6A in gene expression. Among mRNA modification, methylation of internal adenosine N6 positions (m6As) is the most prevalent. The functional impact of m6A modifications largely relies on reader proteins. In this study, we identified OsYTH10, a member of the rice YTH domain family protein, as a key player for recognizing and binding to mRNA m6A modification sites. The m6A-binding activity of OsYTH10 is mediated by its YTH structural domain. Knockout mutation of OsYTH10 results in early flowering in rice. Through FA-CLIP and m6A-seq analysis, we discovered that OsYTH10 binds to m6A in the 3'UTR region of mRNA. Our findings reveal that OsYTH10 stabilizes the mRNAs of target genes OsDTH7 and OsGI, thereby regulating the normal flowering process in rice under prolonged sunlight conditions. This study sheds light on the critical role of OsYTH10 in m6A-mediated gene regulation and its impact on flowering time in rice.Key messageA crucial RNA N6-methyladenosine (m6A) reader protein OsYTH10 in rice was identified to physically binds mRNA's 3'UTR via its YTH domain, stabilizing OsDTH7 and OsGI transcripts to accelerate flowering under long-day conditions.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"256"},"PeriodicalIF":4.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145132036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide meta-QTL approach identifies consensus loci associated with agriculturally important tomato fruit traits.","authors":"Maryam Rashidzade, Moein Shajari, Jacob Barnett, Hamid Razifard, Ana L Caicedo","doi":"10.1007/s00122-025-05033-4","DOIUrl":"https://doi.org/10.1007/s00122-025-05033-4","url":null,"abstract":"<p><strong>Key message: </strong>Using 82 different QTL mapping populations investigated in prior studies, we identified 99 high-confidence meta-QTL regions along with potential candidate genes associated with fruit quality traits in tomato. The genetic analysis of economically important quantitative traits is essential for the development of new cultivars that exhibit improved yield and quality, including disease resistance and enhanced flavor. Meta-QTL (MQTL) analyses allow for the integration of QTL data from multiple independent studies, revealing chromosomal regions consistently associated with traits of interest. In this study, we identified reliable and consistent QTLs for eight agronomically important fruit traits in the cultivated tomato through MQTL analysis of 1,438 QTLs reported in 82 independent mapping populations. Our analysis helped identify 99 consensus MQTLs, distributed across all 12 tomato chromosomes, with almost 4 times narrower confidence intervals (CI) than in the original studies. We identified the most promising candidate genes (CGs) located within each MQTL interval based on the potential function of their orthologous genes in Arabidopsis thaliana. These newly identified CGs complement previously characterized genes and candidate genes with GWAS signals from prior studies. Our research has identified consistent QTLs and CGs in unstressed conditions, offering valuable resources for enhancing fruit-related traits. We further discuss these CGs regarding their potential to improve fruit-related traits in tomato breeding programs.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"255"},"PeriodicalIF":4.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Order from entropy: big data from FAIR data cohorts in the digital age of plant breeding.","authors":"Abhishek Gogna, Daniel Arend, Sebastian Beier, Ehsan Eyshi Rezaei, Tobias Würschum, Yusheng Zhao, Jianting Chu, Jochen C Reif","doi":"10.1007/s00122-025-05040-5","DOIUrl":"10.1007/s00122-025-05040-5","url":null,"abstract":"<p><p>Lack of interoperable datasets in plant breeding research creates an innovation bottleneck, requiring additional effort to integrate diverse datasets-if access is possible at all. Handling of plant breeding data and metadata must, therefore, change toward adopting practices that promote openness, collaboration, standardization, ethical data sharing, sustainability, and transparency of provenance and methodology. FAIR Digital Objects, which build on research data infrastructures and FAIR principles, offer a path to address this interoperability crisis, yet their adoption remains in its infancy. In the present work, we identify data sharing practices in the plant breeding domain as Data Cohorts and establish their connection to FAIR Digital Objects. We further link these cohorts to broader research infrastructures and propose a Data Trustee model for federated data sharing. With this we aim to push the boundaries of data management, often viewed as the last step in plant breeding research, to an ongoing process to enable future innovations in the field.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"257"},"PeriodicalIF":4.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RsRCO and RsLMI1 co-regulate the formation of lobed leaf in radish (Raphanus sativus L.).","authors":"Qian Yang, Lei Cui, Xiaoyao Li, Zhaoxin Peng, Haoyan Sun, Caixia Gan, Zhen Hu, Zhixiong Liu, Zhenbiao Jiao, Zhengming Qiu, Chenghuan Yan, Xiaohui Deng","doi":"10.1007/s00122-025-05044-1","DOIUrl":"https://doi.org/10.1007/s00122-025-05044-1","url":null,"abstract":"<p><strong>Key message: </strong>Adjacent two homeobox genes RsRCO and RsLMI1 co-regulate lobed leaf development in radish and their co-segregating markers are used to breed new lobed-leaf radish varieties. Leaf shape is an important agronomic trait affecting photosynthesis in plants. However, the genetic mechanism of leaf shape in radish (Raphanus sativus L.) remains largely unknown. In this study, an F₂ segregating population was constructed by crossing a lobed-leaf radish 'QZ-16' and an entire-leaf radish '55.' Using bulk segregant analysis and genomic resequencing, we identified Leaf Shape (LS) gene controlling the lobed leaf phenotype in radish. Linkage analysis fine mapped LS locus to a 52.3 kb region on chromosome Rs7. Adjacent two homeobox genes RsRCO and RsLMI1 in this region were determined as the candidate genes, and overexpression of these two genes in Arabidopsis thaliana revealed that they co-regulated the formation of lobed leaf. Furthermore, two co-segregating structure variation markers derived from RsRCO and RsLMI1 genes were developed to genotype the 174 radish accessions, and approximately 80% leaf phenotypes can be explained by the RsLMI1/RsRCO haplotype. GO and KEGG enrichment analyses revealed that the differentially expressed genes were significantly enriched in photosynthesis and phytohormone pathways. Additionally, the contents of cytokinins and auxins were significantly higher in 'QZ-16' than in '55.' Our constructed near-isogenic lobed-leaf NIL-55 line exhibited higher photosynthetic efficiency and biomass than entire-leaf '55.' Finally, a new lobed-leaf variety, 'Chuyu No.15,' was bred from the lobed-leaf cytoplasmic male sterility line 'CLA' using a combination of marker-assisted selection and traditional hybrid breeding methods. Our findings clarified the inheritance regularity of lobed leaf formation in radish and laid a theoretical foundation for further exploring molecular mechanism of leaf development.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"254"},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmCDC7 is involved in coordinating seed size and quality in soybean.","authors":"Ce Yang, Bingbing Li, Hongqiang Yu, Yan Wang, Zhenghong An, Manying Chen, Chaoying He","doi":"10.1007/s00122-025-05039-y","DOIUrl":"https://doi.org/10.1007/s00122-025-05039-y","url":null,"abstract":"<p><strong>Key message: </strong>We revealed essential roles of GmCDC7 in modulating seed size/weight and seed protein/oil content in soybean, presenting potential new targets for improving yield and quality of soybean and other crops. Seed size/weight is a critical factor determining crop yield; however, a limited number of genes regulating this trait have been characterized in soybean. In this study, we identified a Glycine max CELL DIVISION CYCLE 7 (GmCDC7) and revealed its essential roles in seed development. The putative GmCDC7 was highly conserved in both sequences and structure across various species. GmCDC7 transcripts were detectable in multiple tissues, with peak expression occurring during early seed development, while the GmCDC7 proteins were predominantly localized within the nucleus. CRISPR/Cas9-mediated knockout of GmCDC7 led to a significant increase in seed size and 100-seed weight, while overexpression of this gene resulted in a reduction in both seed size and weight. Further cytological analysis demonstrated that GmCDC7 promoted cell expansion and inhibited cell proliferation in seeds. Notably, the gene-edited gmcdc7 mutants showed a substantial increase in protein content alongside a reduction in oil content in seeds. Correspondingly, transcriptomic analyses revealed that GmCDC7 may significantly influence multifaceted regulatory pathways related to cell cycle-related activities, storage protein accumulation, and lipid transport and metabolism during seed development. These findings suggest that GmCDC7 plays pivotal roles in modulating seed size/weight and quality, offering new gene resources and insights into biotechnological strategies for soybean breeding.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"253"},"PeriodicalIF":4.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145087445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomics-enabled dissection of sea wheatgrass genome for advancing wheat genetic resources.","authors":"Dilkaran Singh, Qijun Zhang, Ghana Challa, Elias M Elias, Steven S Xu, Wanlong Li","doi":"10.1007/s00122-025-05021-8","DOIUrl":"10.1007/s00122-025-05021-8","url":null,"abstract":"<p><p>Wheat production is challenged by biotic and abiotic stresses. Alien gene transfer is an effective approach to tackle such challenges. We previously showed that sea wheatgrass (SWG; Thinopyrum junceiforme (2n = 2x = 28; J₁J₂) is an untapped resource possessing resistance to an array of pests and abiotic stress. However, the transfer of these important traits has been hindered by the lack of genomic resources and a clear picture of its genome constitution. Using multi-color genomic in situ hybridization, we distinguished the SWG sub-genomes and corroborated that the J₁ sub-genome is closely related to the E genome of Th. elongatum and the J genome of Th. bessarabicum and the J₂ sub-genome to the V genome of Dasypyrum villosum. Meanwhile, we developed a draft SWG genome assembly and 127 SWG-specific DNA markers covering the 14 SWG chromosomes. Screening a population of 466 BC₂F₁ and BC₂F₂ individuals, derived from backcrosses of wheat-SWG amphiploid to wheat, by the SWG-specific markers led to selection of 72 plants putatively carrying one or two SWG chromosomes. The genome painting analysis of the 72 plants eventually identified a set of 37 wheat-SWG chromosome addition lines covering all the 14 pairs of SWG chromosomes and two compensating Robertsonian translocations (RobTs). While the wheat-SWG chromosome addition lines and RobTs are invaluable genetic resources for wheat improvement via chromosome engineering, our results showed the power of genome-specific markers in combination with genome painting in dissection of a polyploid genome and implicated the origin of a group of important polyploid grasses.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"252"},"PeriodicalIF":4.2,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12446121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homoeologous pairing and recombination features conferred by Aegilops mutica gene system.","authors":"Mingrui Yang, Wen Xiang, Yingjian Zhang, Ziyue Yuan, Guifeng Lu, Wenjuan Yang, Yunfeng Jiang, Ming Hao, Chaolan Fan, Lianquan Zhang, Lin Huang, Shunzong Ning, Bo Jiang, Xuejiao Chen, Xue Chen, Dengcai Liu, Zhongwei Yuan","doi":"10.1007/s00122-025-05037-0","DOIUrl":"https://doi.org/10.1007/s00122-025-05037-0","url":null,"abstract":"<p><p>Aegilops mutica (TT, 2n = 2x = 14) possesses a gene system that counteracts the suppression of homoeologous pairing and recombination by Ph1 gene. In this study, the counteracting effects of the genomes ABT, ABTR, AABBTT, AABBDDTT, and AABBDT were investigated. All harbored the Ph1 gene but exhibited homoeologous pairing. The counteracting effects depend on the constituents of the genome involved. The absence of homologous genomes favored homoeologous pairing, as haploid plants with genomes ABT and ABTR exhibited higher levels of homoeologous pairing than did their corresponding amphidiploid plants with AABBTT. Plants with AABBDT genomes showed higher pairing levels of chromosomes in D-T genomes, but lower pairing levels of chromosomes in D-A/B and T-A/B genomes. The T genome chromosomes more likely to pair with wheat chromosomes in ABTR haploids, whereas the R genome chromosomes were less involved in homoeologous pairing. Interestingly, the T genome showed a higher pairing level with A and D than with B. The most striking feature of the Ae. mutica gene system is that it can induce a high frequency of chromosomal translocations. Of the analyzed F₂ plants derived from the F₁ hybrids with the AABBDT genome, one harbored twelve translocation chromosomes. Unexpectedly, a triple translocation between the A/B-T-D chromosome and two insertional translocations was observed. In practice, its strong ability to induce homoeologous recombination makes the Ae. mutica gene system a new tool for transferring gene from wild relatives to wheat.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"251"},"PeriodicalIF":4.2,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and histological characterization of a dwarf mutant in melon (Cucumis melo L.) reveals potential for breeding semi-dwarf cultivars.","authors":"Zhuoxi Han, Wanning Zheng, Yang Li, Qingqing Ou, Haipeng Zhao, Han Zhao, Ju Xu, Sen Chai, Xueyong Yang, Zhonghua Zhang, Jinjing Sun","doi":"10.1007/s00122-025-05038-z","DOIUrl":"10.1007/s00122-025-05038-z","url":null,"abstract":"<p><strong>Key message: </strong>A missense mutation from proline to leucine at position of 141 in the CmTUB8 protein causes dwarfism in melon. The dwarfing trait is of considerable agronomic importance, as it enables increased planting density and reduced management costs. In melon (Cucumis melo L.), a high-value cucurbit crop, semi-dwarf plant architecture is particularly beneficial for lowering labor requirements in greenhouse trellis cultivation. In this study, a stably inherited dwarf mutant was identified from an ethyl methanesulfonate (EMS)-mutagenized population of thin-skinned inbred line ssp. agrestis '13C'. Using bulked segregant analysis sequencing in combination with MutMap and molecular marker validation, a key missense mutation in the CmTUB8 gene was identified, which completely co-segregates with the dwarf phenotype. This mutation leads to the substitution of proline with leucine at position 141 in the CmTUB8 protein. Histological analysis showed that homozygous mutant stems have significantly reduced vascular bundle size and smaller parenchyma cells. Heterozygous plants exhibited a semi-dwarf phenotype intermediate between the wild-type and homozygous mutants, indicating potential utility in breeding. These findings provide valuable genetic resources and a theoretical basis for the development of dwarf melon cultivars.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"250"},"PeriodicalIF":4.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmWAK3-mediated cell wall remodeling and stomatal dynamics modulate drought tolerance in maize seedlings.","authors":"Qian Yang, Xiaocui Yan, Nan Wang, Tinashe Zenda, Anyi Dong, Xiuzhen Zhai, Yuan Zhong, Mengyun Kou, Zaifeng Li, Huijun Duan","doi":"10.1007/s00122-025-05019-2","DOIUrl":"10.1007/s00122-025-05019-2","url":null,"abstract":"<p><strong>Key message: </strong>ZmWAK3 negatively regulates drought tolerance in maize seedlings by modulating cell wall remodeling and stomatal dynamics, and is directly regulated by the ZmWRKY44 transcription factor. Drought, a significant abiotic stress affecting maize production in key growing regions, necessitates a deeper understanding of its regulatory mechanisms to develop drought-resistant varieties and ensure yield stability. WAKs are pivotal receptor kinases in cell wall signaling, mediating extracellular-to-intracellular communication and participating in diverse processes, including cell expansion, stress adaptation, and pathogen defense. However, the role of WAKs in the drought response remains poorly elucidated. Functional analysis of ZmWAK3 through overexpression and mutant lines has revealed its negative regulatory role in maize drought tolerance. It was found that ZmWAK3 reduces pectin content by increasing polygalacturonase activity, thereby promoting cell wall relaxation. Furthermore, ZmWAK3 was observed to regulate stomatal aperture. Additionally, we demonstrated that the transcription factor ZmWRKY44 directly activates ZmWAK3 expression by binding to a W-box cis-element within its promoter. Crucially, we identified a drought-associated InDel locus within ZmWAK3 and developed the functional marker ZmWAK3-177, which effectively distinguishes drought-tolerant alleles and serves as a practical tool for marker-assisted selection in breeding programs. Collectively, these results reveal a novel mechanism of ZmWAK3 in drought stress response and provide actionable genetic resources for improving maize drought resilience.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"249"},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}