Theoretical and Applied Genetics最新文献

{"title":"An alpha/beta-hydrolase-like gene (GhABH) is identified to be responsible for fiber quality from a multi-effect quantitative trait locus (QTL) on chromosome A06 of upland cotton.","authors":"Sujun Zhang, Jingyan Wei, Liyuan Tang, Cunjing Liu, Xinghe Li, Xiao Cai, Haitao Wang, Jianhong Zhang","doi":"10.1007/s00122-025-05049-w","DOIUrl":"https://doi.org/10.1007/s00122-025-05049-w","url":null,"abstract":"<p><strong>Key message: </strong>GhABH was fine-mapped to a stable multi-effect fiber QTL on chromosome A06 through KASP genotyping and RNA-seq analysis. SNPs in GhABH showed significant associations with fiber traits. GhABH-VIGS simultaneously enhanced fiber length, strength, and fineness by regulating cell wall thickness and helical growth. Arabidopsis overexpression lines exhibited shortened root hairs and reduced stem cell wall thickness. Transcriptome profiling of GhABH-silenced fibers implicated GhNAC and other candidate genes in the GhABH regulatory network. Cotton fiber strength (FS), length (FL), and fineness/micronaire (FM) are economically critical traits, yet the genetic basis for their coordinated regulation remains unclear. Here, we identified a stable quantitative trait locus (QTL) on chromosome A06 associated with both FS and FL. Using KASP markers in a 229-line F_2:3 population derived from the near-isogenic recombinant inbred lines A_dh (exhibiting long, strong fibers) and A_dl (with short, weak fibers), we fine-mapped the QTL to an interval of 11.41 Mb containing 244 genes, including 16 genes differentially expressed during fiber development. Two candidate genes, GhABH and GhMBF1c, showed expression changes with distinct patterns during fiber development. Notably, single-nucleotide polymorphisms in GhABH showed significant associations with FS and FL, underscoring the relevance of this gene for fiber quality. Silencing GhABH in A_dh reduced the fiber diameter but enhanced helix formation and cell wall thickness, ultimately improving FL, FS, and fineness (i.e., reduction in FM). Conversely, GhABH overexpression in Arabidopsis led to shortened root hairs, thinner cell walls in the stem, and fewer vascular bundles. Transcriptomic analysis of GhABH-VIGS fibers revealed potential genes involved in the GhABH regulatory pathway. Collectively, the findings revealed that GhABH is a crucial regulator that simultaneously regulates FL, FS, and fineness and thus represents a promising target for molecular breeding to improve cotton fiber quality.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 11","pages":"268"},"PeriodicalIF":4.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mutation in CmoBADH results in the production of 2-acetyl-1-pyrroline, conferring a 'taro-like' aroma in Cucurbita moschata.","authors":"Gangjun Zhao, Liting Deng, Xiaoxi Liu, Haibin Wu, Jianning Luo, Hao Gong, Xiaoming Zheng, Xueting Wang, Zuyun Dai, Chaoqun Ni, Xiaoshan Wei, Caixia Luo, Junxing Li","doi":"10.1007/s00122-025-05050-3","DOIUrl":"https://doi.org/10.1007/s00122-025-05050-3","url":null,"abstract":"<p><strong>Key message: </strong>We mapped and cloned gene Cmobadh controlling the 'taro-like' aroma in pumpkin, enabling MAS-driven breeding of 'taro-like' aroma cultivars through utilizing the variant-derived molecular marker. 'Taro-like' aroma is a pleasant flavor characteristic of pumpkin. However, the genetic and molecular basis underlying the valuable agronomic trait remains unclear. In this study, an F₂ population was derived from a cross between two pumpkin accessions, including accession NO. 44 with 'taro-like' aroma and accession NO. 45 without 'taro-like' aroma. Genetic analysis revealed that 'taro-like' aroma was controlled by a single recessive gene. Further bulked segregant analysis and polymorphic markers mapped the locus to a 23.3-kb region on chromosome 10. The genomic assembly of NO. 44, generated using third-generation sequencing technology supplemented with Sanger sequencing, revealed a structural variation consisting of a 4,591-bp insertion within the mapped region. The variation is located within an intron of CmoCh10G001620, thereby disrupting its transcription. CmoCh10G001620 encodes betaine aldehyde dehydrogenase and functions as the candidate gene controlling the production of the 'taro-like' aroma compound 2-acetyl-1-pyrroline. Further analysis of 40 plant accessions revealed that the insertion was present in all 10 accessions exhibiting a 'taro-like' aroma, but absent in the other 30 accessions without the special aroma. These findings conclusively identified CmoCh10G001620 as a key gene that regulates 'taro-like' aroma. In addition, molecular marker-assisted selection was successfully used to develop new fragrant pumpkin cultivars that are distinct from conventional pumpkins, through targeted breeding strategies. Our results provide a theoretical foundation for further investigation into the mechanism underlying the 'taro-like' aroma, and may assist in the selection and breeding of pumpkin varieties exhibiting the trait.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 11","pages":"265"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmHSFA2d positively regulates maize seedling heat and drought tolerance by modulating photosystem protein synthesis.","authors":"Yongyan Cao, Chunyu Zhang, Xuanxuan Chen, Tuo Zeng, Hongcheng Wang, Xuye Du, Xun Wu, Bin Zhu, Lei Gu","doi":"10.1007/s00122-025-05052-1","DOIUrl":"https://doi.org/10.1007/s00122-025-05052-1","url":null,"abstract":"<p><strong>Key message: </strong>ZmHSFA2d enhances maize heat and drought tolerance by safeguarding photosynthesis homeostasis, and its expression is directly activated by ZmbHLH124. Heat stress significantly influences plant growth, development, and productivity. Nevertheless, many maize (Zea mays) heat shock factors (HSFs) remain uncharacterized. Here, we explored the role of ZmHSFA2d in thermotolerance. ZmHSFA2d transcript levels were notably elevated under heat shock. ZmHSFA2d localized to the nucleus and displayed transactivation in yeast. Under heat shock, ZmHSFA2d-overexpressing maize seedlings exhibited greater thermotolerance, increased antioxidant enzyme activities, and reduced reactive oxygen species (ROS) and malondialdehyde (MDA) contents compared to control lines; however, these effects were reversed following the CRISPR/Cas9-mediated knockout (KO) of ZmHSFA2d. RNA-Seq analysis revealed that the expression of genes encoding ribosomal proteins, including those associated with ribosome assembly and translation progress, was significantly decreased in ZmHSFA2d-KO plants. Quantitative proteomic analysis further indicated that compared to control, the expression of key proteins [chlorophyll a/b binding proteins and NAD(P)H dehydrogenase (NDH) complex subunits] in photosystem was significantly decreased in ZmHSFA2d-KO lines. Furthermore, using Y1H, EMSA, Dual-Luciferin assays, and maize mesophyll protoplast expression, we found that ZmbHLH124, which is responsive to both heat and drought stress, directly upregulated the transcript levels of ZmHSFA2d. Additionally, ZmHSFA2d positively modulating maize drought tolerance, and compare to WT, ribosomal protein genes in ZmHSFA2d-KO plants also notably decreased under drought stress. Overall, our results indicated that the ZmHSFA2d positively regulates maize heat and drought tolerance by modulating the protein synthesis. This study contributes to the understanding of the role of HSFs in maize thermotolerance and offers useful genetic resources for the breeding of multiple stress-tolerant maize varieties.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 11","pages":"266"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype stacking to improve stability of stripe rust resistance in wheat.","authors":"Jingyang Tong, Zerihun T Tarekegn, Dilani Jambuthenne, Hannah Robinson, Madhav Pandit, Kira Villiers, Sambasivam Periyannan, Lee Hickey, Eric Dinglasan, Ben J Hayes","doi":"10.1007/s00122-025-05045-0","DOIUrl":"10.1007/s00122-025-05045-0","url":null,"abstract":"<p><strong>Key message: </strong>Genotype-by-environment interaction analysis and haplotype-level characterisation provide novel insights into the stability of stripe rust resistance. Breeding selection strategies are proposed to achieve rapid and stable genetic gains across environments. This study investigated stripe/yellow rust (YR) responses in the Vavilov wheat diversity panel evaluated across 11 field experiments conducted in Australia and Ethiopia during 2014-2021. Genotype-by-environment interaction (GEI) was analysed using a factor analytic (FA) model. Genotype-level selection was performed with overall performance (OP) and root-mean-square deviation (RMSD), which reflected average performance and stability of YR resistance across environments, respectively. Genomic estimated breeding values (GEBV) for these traits were calculated and compared with those from a multi-trait GBLUP model with average performance represented by the mean GEBV across environments and stability by the standard deviation of GEBV across environments. The FA-based and multi-trait GBLUP GEBV had high correlations. Haplotypes with large effects on OP and RMSD were identified using the local GEBV method. Favourable haplotypes were then used for stacking in breeding simulations, using the Vavilov collection as a base. Compared to truncation selection, optimal haplotype selection (OHS) using an artificial intelligence (AI)-based algorithm achieved longer-term genetic gains for both OP and RMSD (after many generations) by initially selecting founder parents that maximised favourable haplotypes. Simulations using YR responses from diverse environments that mimicked fluctuating environmental conditions across seasons were conducted to evaluate strategies for selection of YR resistance that is stable across years. Strategies which gave most weight to OP, but some weight to RMSD were optimal in these conditions, and substantially reduced variation of performance across years. This study provides useful information for breeding cultivars with both high YR resistance and high stability of resistance across environments.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 11","pages":"267"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Or-gene-based co-dominant markers and their utilization in marker-assisted breeding for ß-carotene biofortification in different maturity groups of cauliflower.","authors":"Shrawan Singh, Vinay Verma, Bhagchand Shivran, Rahul Kumar Meena, Anamika Chandel, Sandhya Saroha, Navinder Saini","doi":"10.1007/s00122-025-05047-y","DOIUrl":"https://doi.org/10.1007/s00122-025-05047-y","url":null,"abstract":"<p><p>β-Carotene biofortified crops are the most effective and sustainable components in food-based approaches to tackle vitamin A deficiencies in public. Orange cauliflower (Or gene) is one such options; however, co-dominant marker(s) are an essential requirement to deal with a semi-dominant Or gene for foreground selection in marker-assisted breeding. Thus, the present study aimed to develop and validate co-dominant marker(s) for the Or gene and deploy them for rapid recovery of homozygous OrOr progenies in three different maturity groups of Indian cauliflower. Consequently, three crosses from the early (PA/PKVA-1_HM), mid-early (DC 325/PKVA-1_HM) and mid-late maturity (DC 244/PKVA-1_HM) groups were advanced to F₂, F_2:3, BC₂F₁ and BC₁F₂. The segregation pattern of these populations demonstrated the impact of a single semi-dominant Or gene on petiole base pigmentation and curd colour at maturity. The homozygous OrOr genotype was linked to curd deformity. However, these traits were stage-dependent. Two candidate gene markers, namely CFOr-123 and CFOr-346, were designed utilizing the Or-gene BAC sequencing data. These markers were validated in F₂ population and proved useful in distinguishing homozygous and heterozygous individuals in F₂ and BC₁F₂. Carotenoid content was highest in DC 244/ PKVA-1_HM followed by DC 325/ PKVA-1_HM and PA/ PKVA-1_HM. A total of 16 promising homozygous F_2:3 progenies were developed in tropical cauliflower backgrounds coupled with two Or-gene-based co-dominant markers, which are crucial in managing the ß-carotene biofortification programme of cauliflower.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"264"},"PeriodicalIF":4.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting seed composition QTL from wild soybean: fine-mapping, candidate gene identification, and evaluation of introgression effects on agronomic performance.","authors":"Elizabeth De Meyer, Anser Mahmood, Mariola Usovsky, Jason Gillman, Bing Yang, Jared Decker, Qijian Song, Clinton Meinhardt, Michael Dennigmann, Asheesh K Singh, George Graef, Eliana Monteverde, Andrew Scaboo","doi":"10.1007/s00122-025-05048-x","DOIUrl":"10.1007/s00122-025-05048-x","url":null,"abstract":"<p><strong>Key message: </strong>Seed composition QTL from wild soybean were confirmed and validated in two genetic backgrounds across multiple environments, candidate genes were identified, and agronomic performance of backcross introgression lines was evaluated. Through selection for soybean yield, breeders have inadvertently reduced seed protein content and increased oil due to phenotypic and genetic correlations between these three traits. Therefore, identifying alleles that increase protein without adversely affecting oil and yield is of interest for breeders and the entire soybean value chain. Previously, a G. max × G. soja population was used to map a protein-associated region to ~ 4.6 Mbp on chromosome (Chr) 14. The G. soja allele significantly increased protein 6.5-7.2 g kg^-1, without significantly decreasing oil. Additionally, two oil quantitative trait loci (QTL) were reported on Chrs 8 and 14. In this study, we aimed to confirm the Chr 14 protein QTL, evaluate QTL effects on seed composition and agronomic performance, and further fine-map to identify candidate genes. We validated and fine-mapped the Chr 14 protein QTL to a 0.6 Mbp region in a different genetic background, where the G. soja allele significantly increased protein by 9.3 g kg^-1. Further, we confirmed the Chr 14 oil QTL linked to the protein QTL and the Chr 8 oil QTL. Chr 14 protein QTL effects on agronomic traits were evaluated in a backcross population across eight environments. The QTL significantly increased protein content, without significantly impacting oil, maturity, or plant height. While the QTL impacted yield and lodging, its effect and significance varied within environments. The candidate genes identified for these three validated seed composition QTL, along with additional molecular markers developed, offer valuable resources for improving seed composition in soybean breeding programs.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"263"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic prediction-aided incorporation of genetic resources into elite breeding: lessons from a collaborative multiparental design in flint maize.","authors":"Dimitri Sanchez, Sarah Ben Sadoun, Tristan Mary-Huard, Cyril Bauland, Carine Palaffre, Bernard Lagardère, Delphine Madur, Valérie Combes, Stéphane Melkior, Laurent Bettinger, Alain Murigneux, Laurence Moreau, Alain Charcosset","doi":"10.1007/s00122-025-05034-3","DOIUrl":"10.1007/s00122-025-05034-3","url":null,"abstract":"<p><strong>Key message: </strong>A public private cooperative mating design between elite maize inbred lines and diversity donors shows that genomic prediction holds great promise to improve the use of genetic resources. Genetic diversity is essential for plant breeding, enabling long-term gains and adaptation to climate change and new agronomical practices. Breeders can access diverse genetic resources to enhance elite germplasm and introduce new favorable variations. The limited performance of genetic resources may hamper their use. To overcome this, a bridging population can be implemented to evaluate and select progenies from crosses between diversity donors and elite lines before their introduction in breeding programs. The choice of such crosses can be dealt with the usefulness criterion (UC), which determines its ability to produce transgressive individuals. This paper investigates the use of genome-wide marker effects to predict (i) the performance of individuals derived from crosses between donors and elite lines and (ii) the UC of crosses not observed yet. It also compares donor introduction strategies based on the UC or the H criterion, which considers the genome-wide donor-elite complementarity. We used a flint maize collaborative multi-parental BC1-S2 population, consisting in materials from six breeding companies and one public institute crossed to different donors. The 20 crosses had contrasted means and genetic variances, and most of them presented transgressive individuals above the elite parent. Results emphasize the importance of half-siblings derived from the elite line parent of the predicted cross to efficiently predict progeny performances or the UC. They also showed that using the H criterion appears promising to select iteratively donors that best complement initial elite materials. The paper concludes with guidelines for implementing a bridging population using genome-wide marker-based predictions.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"262"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL mapping using a high-density SNP map and development of KASP markers for MYMIV resistance in mungbean (Vigna radiata L.).","authors":"Chayanika Lahkar, Ranjit Kaur Gill, Sandeep Kaur Dhaliwal, Parteek Kumar, Deepika Narang, Dharminder Bhatia, Asmita Sirari, Satinder Kaur","doi":"10.1007/s00122-025-05046-z","DOIUrl":"https://doi.org/10.1007/s00122-025-05046-z","url":null,"abstract":"<p><p>Mungbean yellow mosaic India virus (MYMIV) poses a severe threat to mungbean (Vigna radiata L.) cultivation, leading to substantial yield losses across tropical and subtropical regions. The present study was aimed to identify genomic location(s) governing MYMIV resistance in the resistant source of mungbean, ML1808. Inheritance studies from the crossSML 1082 × ML 1808in mungbean indicated the presence of a single dominant gene governing MYMIV trait in both the crosses. Leveraging genotyping-by-sequencing platform, a high-density linkage map was constructed in mungbean using F₂ individuals derived from cross SML 1082 × ML 1808.The map contained 2638 single nucleotide polymorphism (SNP) markers distributed in 11 linkage groups and spanned for 1924.279 cM length with an average marker distance of 0.729 cM. The individual linkage group ranged from 129.3 to 207.6 cM in length, with 102-395 SNP markers per chromosome. The QTL analysis using the genetic map and F₃ and F₄ phenotyping data identified a major QTL, designated as qMYMIV3_3 at 5 cM position on chromosome 3, common in both the populations. This QTL mapped for MYMIV resistance in mungbean variety ML1808 harbor possible candidate genes for controlling MYMIV resistance. Linkage mapping using KASP (Kompetitive Allele-Specific Polymorphism) markers developed from potent candidate genes involved in disease resistance identified the 380 kb genomic region linked with MYMIV. The two KASP markers closely associated with MYMIV, viz. Vrad305 and Vrad308 originated from RPL19 (ribosomal protein L19-1) and WRKY transcription factor genes, respectively. The constructed genetic linkage map and QTL/gene (s) along with the closely linked markers for MYMIV resistance identified in this study will be important for marker-assisted breeding programs.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"261"},"PeriodicalIF":4.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic basis and role of exotic accessions in cultivated cotton fiber quality improvement.","authors":"Ankush Sharma, Mingrui Xu, Deepak Vitrakoti, Jinesh D Patel, Peng W Chee, Andrew H Paterson","doi":"10.1007/s00122-025-05043-2","DOIUrl":"10.1007/s00122-025-05043-2","url":null,"abstract":"<p><strong>Key message: </strong>Exotic Gossypium accessions still harbor QTL‑validated alleles that, combined with CRISPR pyramiding and genomic selection, can break the entrenched fiber length-strength trade‑off. Cotton's four independent domestications twice in diploids and twice in allotetraploids offer a natural experiment in fiber improvement. Synthesizing three decades of data, we chart how polyploidy, selection and modern breeding have repeatedly reshaped the Gossypium genome. More than 15,000 quantitative trait locus (QTL) and genome wide association mapping studies (GWAS) hits converge on a handful of chromosomal \"hotspots\"; new MAGIC, NAM, NIL and long-read resources now narrow these peaks to < 200 kb, resolving causal genes such as GhHOX3, GhZF14 and GhMYB7. Multi-omics evidence links auxin, ethylene, gibberellin, brassinosteroid and strigolactone signaling to HDZIP IV, MYB, bHLH/HLH and ERF networks that drive fiber initiation, extreme cell elongation and cellulose deposition. Population genomics shows that ~ 40% of favorable fiber alleles are fixed in elite Gossypium hirsutum, yet wild diploids and landraces still harbor variants that could break the length strength trade-off. We propose a three-step roadmap genomic selection, CRISPR gene pyramiding and accelerated introgression to expand cotton's genetic base and deliver fibers suited to sustainable textile demands.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"260"},"PeriodicalIF":4.2,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A high‑throughput pipeline for phenotyping, object detection and quantification of leaf trichomes.","authors":"Andrea González-Muñoz, Dai-Jie Wu, Ana B Perera-Rodríguez, Mohamed Rekik, Silvio Giancola, Brande B H Wulff, Catherine Gardener","doi":"10.1007/s00122-025-05035-2","DOIUrl":"10.1007/s00122-025-05035-2","url":null,"abstract":"","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 10","pages":"259"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}