{"title":"云南脱壳小麦成株抗条锈病基因的分子鉴定与验证。yunnanense王)。","authors":"Linyu Huang, Xinyu Wang, Yan Yan, Li Long, Qiushuo Li, Yuexue Wang, Yongjing Liu, Hao Li, Zhien Pu, Wei Li, Qiantao Jiang, Jian Ma, Houyang Kang, Pengfei Qi, Qiang Xu, Yazhou Zhang, Mei Deng, Yuming Wei, Guoyue Chen, Yunfeng Jiang","doi":"10.1007/s00122-025-04906-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Three stable QTLs for adult-plant resistance to stripe rust were identified on chromosomes 2DL, 5B, and 7BS in Yunnan hulled wheat, providing durable high-level resistance through additive effects. Yunnan hulled wheat, a unique subspecies native to China, exhibits excellent adult-plant resistance (APR) to stripe rust. However, the genetic basis of stripe rust resistance in Yunnan hulled wheat remains largely unexplored. In this study, we investigated an accession from Lancang County, which has shown stable APR under field conditions for over 10 years. To identify the stripe rust gene of Lancang hulled wheat (LC), we developed 108 F<sub>5:7</sub> recombinant inbred lines (RILs) from the cross LC × Avocet S, followed by multi-environment phenotypic evaluation of APR to stripe rust. Bulked segregant exome capture sequencing and quantitative trait locus (QTL) analysis identified three stable QTLs on chromosomes 2DL (QYr.LC-2DL), 5B (QYr.LC-5B), and 7BS (QYr.LC-7BS), explaining 11.44-15.74%, 11.27-15.31%, and 12.12-20.00% of the phenotypic variance, respectively. To validate these QTLs, we constructed three independent heterogeneous inbred families (HIF) segregating for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS from the RIL populations using QTL-linked molecular markers. Further molecular mapping of these three HIF populations refined the localization of QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS to intervals between markers KP2D-617.34 and KP2D-625.65, KP5B-121.75 and KP5B-262.42, and KP7B-52.54 and KP7B-58.54, respectively. Additionally, additive effects were observed for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS, with lines carrying all three loci showing the highest resistance. Moreover, the linked markers of QYr.LC-2DL and QYr.LC-7BS can be effectively utilized for marker-assisted selection in wheat breeding programs. These findings provide valuable insights into the genetic basis of stripe rust resistance in Yunnan hulled wheat and offer germplasm for breeding wheat varieties with durable resistance.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 6","pages":"116"},"PeriodicalIF":4.4000,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization and validation of adult-plant stripe rust resistance genes in Yunnan hulled wheat (Triticum aestivum ssp. yunnanense King).\",\"authors\":\"Linyu Huang, Xinyu Wang, Yan Yan, Li Long, Qiushuo Li, Yuexue Wang, Yongjing Liu, Hao Li, Zhien Pu, Wei Li, Qiantao Jiang, Jian Ma, Houyang Kang, Pengfei Qi, Qiang Xu, Yazhou Zhang, Mei Deng, Yuming Wei, Guoyue Chen, Yunfeng Jiang\",\"doi\":\"10.1007/s00122-025-04906-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Key message: </strong>Three stable QTLs for adult-plant resistance to stripe rust were identified on chromosomes 2DL, 5B, and 7BS in Yunnan hulled wheat, providing durable high-level resistance through additive effects. Yunnan hulled wheat, a unique subspecies native to China, exhibits excellent adult-plant resistance (APR) to stripe rust. However, the genetic basis of stripe rust resistance in Yunnan hulled wheat remains largely unexplored. In this study, we investigated an accession from Lancang County, which has shown stable APR under field conditions for over 10 years. To identify the stripe rust gene of Lancang hulled wheat (LC), we developed 108 F<sub>5:7</sub> recombinant inbred lines (RILs) from the cross LC × Avocet S, followed by multi-environment phenotypic evaluation of APR to stripe rust. Bulked segregant exome capture sequencing and quantitative trait locus (QTL) analysis identified three stable QTLs on chromosomes 2DL (QYr.LC-2DL), 5B (QYr.LC-5B), and 7BS (QYr.LC-7BS), explaining 11.44-15.74%, 11.27-15.31%, and 12.12-20.00% of the phenotypic variance, respectively. To validate these QTLs, we constructed three independent heterogeneous inbred families (HIF) segregating for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS from the RIL populations using QTL-linked molecular markers. Further molecular mapping of these three HIF populations refined the localization of QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS to intervals between markers KP2D-617.34 and KP2D-625.65, KP5B-121.75 and KP5B-262.42, and KP7B-52.54 and KP7B-58.54, respectively. Additionally, additive effects were observed for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS, with lines carrying all three loci showing the highest resistance. Moreover, the linked markers of QYr.LC-2DL and QYr.LC-7BS can be effectively utilized for marker-assisted selection in wheat breeding programs. These findings provide valuable insights into the genetic basis of stripe rust resistance in Yunnan hulled wheat and offer germplasm for breeding wheat varieties with durable resistance.</p>\",\"PeriodicalId\":22955,\"journal\":{\"name\":\"Theoretical and Applied Genetics\",\"volume\":\"138 6\",\"pages\":\"116\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Theoretical and Applied Genetics\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s00122-025-04906-y\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Applied Genetics","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s00122-025-04906-y","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Molecular characterization and validation of adult-plant stripe rust resistance genes in Yunnan hulled wheat (Triticum aestivum ssp. yunnanense King).
Key message: Three stable QTLs for adult-plant resistance to stripe rust were identified on chromosomes 2DL, 5B, and 7BS in Yunnan hulled wheat, providing durable high-level resistance through additive effects. Yunnan hulled wheat, a unique subspecies native to China, exhibits excellent adult-plant resistance (APR) to stripe rust. However, the genetic basis of stripe rust resistance in Yunnan hulled wheat remains largely unexplored. In this study, we investigated an accession from Lancang County, which has shown stable APR under field conditions for over 10 years. To identify the stripe rust gene of Lancang hulled wheat (LC), we developed 108 F5:7 recombinant inbred lines (RILs) from the cross LC × Avocet S, followed by multi-environment phenotypic evaluation of APR to stripe rust. Bulked segregant exome capture sequencing and quantitative trait locus (QTL) analysis identified three stable QTLs on chromosomes 2DL (QYr.LC-2DL), 5B (QYr.LC-5B), and 7BS (QYr.LC-7BS), explaining 11.44-15.74%, 11.27-15.31%, and 12.12-20.00% of the phenotypic variance, respectively. To validate these QTLs, we constructed three independent heterogeneous inbred families (HIF) segregating for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS from the RIL populations using QTL-linked molecular markers. Further molecular mapping of these three HIF populations refined the localization of QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS to intervals between markers KP2D-617.34 and KP2D-625.65, KP5B-121.75 and KP5B-262.42, and KP7B-52.54 and KP7B-58.54, respectively. Additionally, additive effects were observed for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS, with lines carrying all three loci showing the highest resistance. Moreover, the linked markers of QYr.LC-2DL and QYr.LC-7BS can be effectively utilized for marker-assisted selection in wheat breeding programs. These findings provide valuable insights into the genetic basis of stripe rust resistance in Yunnan hulled wheat and offer germplasm for breeding wheat varieties with durable resistance.
期刊介绍：
Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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