Reviews in Aquaculture最新文献

{"title":"Nitrite Toxicity in Shrimp Aquaculture: Mechanisms, Health Impacts, and Sustainable Mitigation Strategies","authors":"Dan-Dan Bian,&nbsp;Yan-Xia Shi,&nbsp;Xue Zhang,&nbsp;Xin Liu,&nbsp;Jun-Jie Jiang,&nbsp;Xi-Rong Zhu,&nbsp;Dai-Zhen Zhang,&nbsp;Qiu-Ning Liu,&nbsp;Bao-Jian Zhu,&nbsp;Bo-Ping Tang","doi":"10.1111/raq.70062","DOIUrl":"https://doi.org/10.1111/raq.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>The rapid expansion of intensive aquaculture systems has caused escalating environmental challenges due to excessive nitrogenous waste accumulation, which surpasses the metabolic capacity of aquatic microbiota and elevates nitrite concentrations. As a pervasive environmental toxin, nitrite induces oxidative stress, disrupts metabolic homeostasis, and compromises gut microbiota and immune function in shrimp, leading to mass mortality, economic losses, and ecological disruption. These impacts threaten both aquaculture productivity and ecological sustainability. This review synthesizes three decades of research to elucidate the multifactorial mechanisms of nitrite toxicity, including genotoxicity, apoptosis, growth impairment, and dysregulated cellular signaling pathways. Key findings highlight that nitrite overload impairs mitochondrial function, dysregulates apoptosis pathways, and interferes with growth and reproductive processes, thereby undermining shrimp development and reproduction. To address these challenges, the review evaluates emerging mitigation strategies such as antioxidant-enriched feeds, probiotic modulation, and biofloc technology, which optimize nitrogen cycling, enhance detoxification capacity, restore microbial equilibrium, and improve survival rates in experimental trials. Unlike prior fragmented studies, this review integrates mechanistic insights into nitrite toxicity with scalable interventions, proposing a novel decision framework for system-specific nitrite management. This framework offers actionable recommendations for policymakers and industry stakeholders to balance aquaculture productivity with ecological sustainability. By advancing the understanding of nitrite's ecotoxicological impacts, this review charts a path toward resilient and sustainable aquaculture systems, transforming mechanistic insights into preventive protocols and offering a holistic perspective on nitrite pollution control that reconciles economic objectives with ecological sustainability.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Medicinal Herbs and Plants to Control Ciliate Infection in Finfish for Sustainable Aquaculture: A Review","authors":"Soo Ji Woo","doi":"10.1111/raq.70063","DOIUrl":"https://doi.org/10.1111/raq.70063","url":null,"abstract":"<div>\u0000 \u0000 <p>Ciliate protozoa are highly contagious pathogens causing significant economic losses in global finfish aquaculture. While conventional chemotherapeutics like formalin offer control, their overuse raises environmental and safety concerns, creating an urgent need for sustainable alternatives. This review systematically synthesizes research from the past two decades, evaluating the antiparasitic efficacy of 29 medicinal plants against major ciliate pathogens such as <i>Ichthyophthirius multifiliis</i> and <i>Cryptocaryon irritans</i>. The analysis reveals that numerous plants, particularly <i>Sophora flavescens</i>, <i>Cynanchum atratum</i>, and <i>Curcuma longa</i>, exhibit exceptional efficacy. Their bioactive compounds, including alkaloids, flavonoids, and tannins, effectively eliminate parasites by disrupting cell membranes and metabolic pathways, often with substantial safety margins compared to chemical drugs. Despite this potential, significant barriers hinder their practical application. These include a scarcity of large-scale field trials, a lack of standardized formulations leading to inconsistent results, and complex, divergent regulatory hurdles that impede commercialization. This review concludes that while medicinal plants are a highly promising tool for sustainable aquaculture, realizing their full potential requires a concerted effort to bridge the gap between laboratory research and practical, regulated application through field validation and standardized product development.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Seriola lalandi Aquaculture With a Focus on Recirculating Aquaculture Systems: Synthesis of Existing Research and Emerging Challenges","authors":"Alexander Chong Shu-Chien,&nbsp;Alvin Setiawan,&nbsp;Mark Camara,&nbsp;Carly Wilson,&nbsp;Andrew Forsythe,&nbsp;Steve Pether,&nbsp;Dave McQueen,&nbsp;Glen Irvine,&nbsp;Yann Gublin","doi":"10.1111/raq.70059","DOIUrl":"https://doi.org/10.1111/raq.70059","url":null,"abstract":"<p>This review presents a comprehensive synthesis of research on yellowtail kingfish (<i>Seriola lalandi</i>) aquaculture, with a particular focus on production in recirculating aquaculture systems (RAS). Significant progress has been made in reproductive biology and captive spawning, which are critical for ensuring a reliable juvenile supply. Key factors influencing larval development, including temperature, photoperiod, and feeding strategies, are reviewed alongside nutritional requirements for optimal growth and health. The efficacy of alternative feed ingredients aimed at reducing dependence on fish meal and fish oil is discussed, along with issues related to digestibility, fecal waste management, and nutrient retention in RAS. The review explores studies on the <i>S. lalandi</i> microbiota, highlighting associations between microbial composition and predicted functional profiles under varying RAS parameters. Physiological traits that support the species' suitability for RAS, such as resilience to handling stress and environmental fluctuations, are evaluated with an emphasis on tolerance to carbon dioxide, ammonia, salinity, and oxygen levels. The species' thermal sensitivity and implications for metabolic performance are also addressed. Advancements in genetic research are discussed, including the development of microsatellite markers, single nucleotide polymorphisms, and linkage maps. Findings in taxonomy, phylogeography, and population genetics are reviewed with a focus on potential applications in selective breeding for production traits. The review also covers current and emerging pathogens affecting <i>S. lalandi</i>, the risk of outbreaks in RAS, and key welfare considerations. An economic outlook for yellowtail kingfish production in RAS highlights the need for integrated approaches for sustainable and cost-effective aquaculture of <i>S. lalandi</i>.</p>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/raq.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energetic Adaptations of Bivalves Under Environmental Stress: A Comprehensive Review on Bioenergetics and Aquaculture Sustainability","authors":"Chunhua Liu,&nbsp;Yueyong Shang,&nbsp;Shanza Gul,&nbsp;Menghong Hu,&nbsp;Youji Wang","doi":"10.1111/raq.70052","DOIUrl":"https://doi.org/10.1111/raq.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioenergetics, or scope for growth (SFG), is a model used to assess the health and adaptability of mollusks to external factors. It achieves this by converting physiological responses, such as clearance rate (CR), respiration rate (RR), excretion rate (ER), absorption efficiency (AE), and oxygen–nitrogen ratio (O:N ratio), into energy equivalents. This biomarker reflects the energy available for growth and reproduction, indicating an organism's potential to adapt to environmental change and revealing the energetics behind growth. It has been a significant focus in marine ecology, biology, and aquaculture of mollusks. To understand the effects of various factors on the bioenergetics of bivalves, the effects of temperature, ocean acidification (OA), salinity, dissolved oxygen (DO), trace elements (e.g., copper (Cu), zinc (Zn), cadmium (Cd)), micro/nanoparticles (MPs/NPs), harmful algae, and other factors on the physiology and energy budget of bivalves were summarized and analyszed in this paper. We found that some influencing factors (e.g., OA, trace elements, and harmful algae) serve as selective pressures that make bivalves adapt to environmental changes, and the impacts of various stressors on the SFG in bivalves are diverse. However, under hypoxia and salinity changes, exceeding bivalve tolerance can lead to metabolic disorders and reduced SFG, impacting populations and aquaculture. At the same times, we analyzed WOS literature from 1980 to 2023 using VOS-viewer software to assess how climate change and pollutants affect bivalve energy assimilation and consumption, offering insights for bivalve biology and aquaculture research.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asian Seabass (Lates calcarifer): A Versatile Species for Marine and Freshwater Aquaculture","authors":"Gen Hua Yue","doi":"10.1111/raq.70054","DOIUrl":"https://doi.org/10.1111/raq.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Aquaculture has undergone unprecedented growth in recent decades, driven by the increasing global demand for seafood. However, this expansion brings environmental challenges and underscores the need for species diversification and sustainable farming practices. Among the rising stars of aquaculture, the Asian seabass (<i>Lates calcarifer</i>) stands out as a prime candidate due to its remarkable adaptability, high market value, and strong biological traits. This review provides an in-depth exploration of eight key aspects of Asian seabass aquaculture: (1) species biology and distribution, (2) the current status of its global aquaculture, (3) nutritional value and health benefits, (4) genetic improvement through selective breeding, molecular techniques, and genome editing, (5) environmental sustainability and ecological impact, (6) disease management and health challenges, (7) market trends and economic viability, and (8) future prospects and research directions. Advances in genetics and breeding technologies have significantly enhanced key traits such as rapid growth, disease resistance, and omega-3 content, further cementing its role as a species of high commercial importance. To fully unlock its potential, ongoing research in disease prevention, climate resilience, optimized nutrition, and advanced breeding strategies is crucial. Coupled with strategic investments in farming technologies, branding, and market expansion, Asian seabass is well-positioned to become a versatile species of both marine and freshwater aquaculture.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotonin: A Multifunctional Molecule in Crustaceans","authors":"Noah Esmaeili,&nbsp;Sunil Kadri,&nbsp;Vikas Kumar,&nbsp;Hongyu Ma","doi":"10.1111/raq.70046","DOIUrl":"https://doi.org/10.1111/raq.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Serotonin is an ancient monoamine neurotransmitter and hormone that has existed for millions of years, from prokaryotic life forms to modern humans. It is also commonly known as 5-hydroxytryptamine (5-HT). In mammals, it is well known for its actions in depression, aggression, anxiety, stress, and sexual behavior. In crustaceans, this molecule plays some vital roles in agonistic behavior, which is the main cause of economic loss in many crustacean (e.g., crayfish and crab) farms. 5-HT is commonly used to induce reproduction and can potentially be used as an alternative treatment for unilateral eyestalk ablation. This neurotransmitter has direct effects on development, molting, growth, feeding, digestion, and gut microbiota. It is also the main regulator of stress response, osmoregulation, and, to a lesser extent, energy homeostasis in crustaceans. Further, the immune and antioxidant systems and intermediary metabolism are affected by 5-HT administration. 5-HT regulates almost all physiological mechanisms in crustaceans. Antidepressant drugs that inhibit 5-HT reuptake are used for treating behavioral disorders, but their toxicological impacts on the environment are a severe threat. Despite this massive range of actions, no review has ever been conducted to provide an overview of 5-HT roles in any aquatic species. In this review, these physiological mechanisms are discussed, and suggestions for future directions for research and aquaculture are provided.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Issues of Micro-Nanoplastics in the Oyster and Mussel Aquaculture Sector","authors":"Arnaud Huvet,&nbsp;Morgane Le Gall,&nbsp;Hippolyte Gilante,&nbsp;Ika Paul-Pont","doi":"10.1111/raq.70047","DOIUrl":"https://doi.org/10.1111/raq.70047","url":null,"abstract":"<div>\u0000 \u0000 <p>Over 90% of plastic debris in marine environments consists of microplastics smaller than 5 mm. This is a growing global concern, especially given the emerging but largely unquantified presence of nanoplastics. This review synthesizes current knowledge on micro- and nanoplastic contamination in coastal areas and its effects on bivalve species, particularly oysters and mussels, whether in aquaculture or the wild. Drawing on 316 references, notably case studies of (micro)plastic waste contamination of surface waters, sediments, and animals in coastal areas, and experimental data on ingestion, fate, and impacts of micro-nanoplastics on organisms. Microplastic levels range from a few particles per cubic meter of seawater (&gt; 300 μm) to several hundred per kilogram of sediment. Between 60% and 93% of oysters and mussels are contaminated, with concentrations ranging from 0.2 to 2.9 particles per gram of tissue; over 80% of these particles are smaller than 50 μm. After ingestion, most microplastics are excreted, but smaller nanoplastics (&lt; 100 nm) can cross biological membranes and reach internal organs. Even transient exposure in the digestive tract can impair energy uptake, immune function, and reproduction. Plastic additives can also cause endocrine-like disruptions. Reducing plastic pollution in coastal environments requires coordinated action. Effective actions should include integrated and enforceable strategies, including international regulations on plastic additives, improved waste management, and the promotion of safer alternative materials.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating Nitrofuran Challenges in Farmed Prawn (Macrobrachium rosenbergii): Strengthening Safety and Export Potentials in Bangladesh","authors":"S. M. Nazmul Alam","doi":"10.1111/raq.70041","DOIUrl":"https://doi.org/10.1111/raq.70041","url":null,"abstract":"<p>Nitrofuran metabolites, including semicarbazide, pose significant risks to food safety, particularly in the aquatic industry. Bangladesh's shrimp industry faced major challenges due to nitrofuran contamination, leading to a voluntary export suspension to the European Union (EU). Despite improved hygiene practices, significant levels of nitrofuran residues were detected in prawns, leading to numerous EUs Rapid Alert System for Food and Feed (RASFF) notifications in 2009. This crisis has led to economic losses, job reductions, and damage to Bangladesh's international reputation. In response, Bangladesh implemented a comprehensive national plan, including a temporary export ban, extensive testing, and significant regulatory reforms. The government focused on improving laboratory testing capabilities, establishing new laboratories, and enhancing the monitoring of shrimp production. Additionally, extension materials and training programs were developed to promote sustainable aquaculture practices and reduce antibiotic use. The collaborative efforts between the government and industry stakeholders have improved compliance with international food safety standards, restored confidence in Bangladesh's shrimp exports, reduced RASFF notifications and non-compliant samples under the national residue control plan. These achievements emphasized the critical importance of continued vigilance and sustainability in maintaining and expand global market access for the Bangladesh's shrimp industry.</p>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/raq.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Progress of the Long-Term Preservation for Fish Embryos, Female Reproductive Cells, and Tissues","authors":"Wei Qin,&nbsp;Jiaji Pan,&nbsp;Jun Xiao,&nbsp;Hao Feng","doi":"10.1111/raq.70057","DOIUrl":"https://doi.org/10.1111/raq.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>Cryopreservation is an effective technique to preserve biomaterials for a very long time period. The long-term preservation techniques benefit the rapid development of the aquaculture industry, such as optimizing the breeding schemes. Currently, many cryopreservation attempts have been conducted on fish embryos and gametes. The cryopreservation of fish sperms is the most successful among these studies due to the simple structure of sperms. However, the cryopreservation of female gametes is developing at a much slower pace. In this work, the techniques and progress related to the cryopreservation of fish embryos, blastomeres, oocytes, testicular tissue, ovarian tissues, and primordial germ cells (PGCs) are introduced. This review first discusses the cryoprotective agents (CPAs) used in the cryopreservation or vitrification of fish embryos and gametes. Then we summarize advanced techniques including the pretreatment, rewarming methods, removal of CPAs, and assessment methods, followed by introducing the preservation results. In the end, some suggestions are raised for the future development of fish embryos, germ cells, and gonadal tissues.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Situ Biofilm Aquaculture Systems (In Situ BFSs) for Litopenaeus vannamei: A Review","authors":"Wei Zhu,&nbsp;Guangnian Yuan,&nbsp;Zhiwen Song,&nbsp;Qiufen Li,&nbsp;Chao Liu,&nbsp;Ailing Xu,&nbsp;Yazhi Luan,&nbsp;Yongxin Liu","doi":"10.1111/raq.70058","DOIUrl":"https://doi.org/10.1111/raq.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>The scale of intensive <i>Litopenaeus vannamei</i> aquaculture has increased over the past few decades. However, the inorganic nitrogen and suspended solids (SSs) generated during the aquaculture process are toxic to shrimp and have negative environmental effects. Therefore, removing these pollutants from aquaculture systems is a critical issue that must be addressed. In addition to the widely used recirculating aquaculture systems (RASs) and biofloc technology (BFTs), the addition of submerged substrates to <i>L. vannamei</i> aquaculture tanks to construct in situ biofilm aquaculture systems (in situ BFSs) is an alternative that results in favorable water quality and enables low or zero water exchange, significantly improving shrimp performance and decreasing the feed conversion ratio (FCR). This review highlights the principles, construction methods, water treatment effectiveness, and impact of in situ BFSs on <i>L. vannamei</i> growth as well as pathogen control. The selection of appropriate substrates and methods for nitrifying biofilm cultivation and substrate fixation can significantly affect water treatment efficiency, reduce the concentrations of harmful substances (SSs, ammonia and nitrite) and provide additional nutrients and habitats for <i>L. vannamei</i>, thereby increasing their survival rate and enhancing their growth performance. In addition, the integration of in situ BFSs with BFTs and algal-symbiotic systems is discussed. In this paper, the potential for further increasing the treatment capacity of in situ BFSs through sulfur autotrophic denitrification (SAD) and electric field-assisted biofilm systems is proposed. The application of these innovative technologies is expected to provide new insights and solutions for achieving sustainable development in <i>L. vannamei</i> aquaculture.</p>\u0000 </div>","PeriodicalId":227,"journal":{"name":"Reviews in Aquaculture","volume":"17 4","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}