The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)最新文献_第6页

Using TD-GC-MS to analyze coffee beans aromas of different roast levels 采用TD-GC-MS对不同烘焙水平的咖啡豆香气进行分析

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908860

Chia-Hui Li, L. Chieng, Irrigepalli Srinivasa Thimmaraju, D. Yao

引用次数: 5

The application of a new beam-rigid body MEMS gyroscope in the frequency-modulation mode 一种新型波束刚体MEMS陀螺仪在调频模式下的应用

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908880

S. A. M. Lajimi, G. Heppler, E. Abdel-Rahman

引用次数: 4

Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system 在集成微流控系统上使用适体/抗体测定糖化血红蛋白

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908895

K. Chang, Jing-Lun Li, Ching-Hsuan Yang, S. Shiesh, Gwo-Bin Lee

{"title":"Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system","authors":"K. Chang, Jing-Lun Li, Ching-Hsuan Yang, S. Shiesh, Gwo-Bin Lee","doi":"10.1109/NEMS.2014.6908895","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908895","url":null,"abstract":"Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"31 1","pages":"647-650"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87112241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Digital fast startup procedure for micro-machined vibratory gyroscopes using optimized fuzzy control strategy 基于优化模糊控制策略的微加工振动陀螺仪数字快速启动程序

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908792

Long Wang, Qiancheng Zhao, Minghao Nie, Dachuan Liu, Yuxian Liu, Junjie Yan, Zhenchuan Yang, G. Yan

引用次数: 0

Multi-antibody array chip for immunoassay of different-grade bladder cancer cell lysates based on DEP manipulations of nanoprobes 基于纳米探针DEP操作的不同级别膀胱癌细胞裂解物免疫分析的多抗体阵列芯片

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908889

C. Chuang, Jing-Wei Ju, Kai-Chieh Chang, Yao-Wei Huang, C. Jen

{"title":"Multi-antibody array chip for immunoassay of different-grade bladder cancer cell lysates based on DEP manipulations of nanoprobes","authors":"C. Chuang, Jing-Wei Ju, Kai-Chieh Chang, Yao-Wei Huang, C. Jen","doi":"10.1109/NEMS.2014.6908889","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908889","url":null,"abstract":"This paper develops a multi-antibody array biochip for detection of different-grade bladder cancer cell lysates based on protein expression level. Two nanoprobes modified with antibodies, Galectin-1 (Gal-1) and Lactate Dehydrogenase B (LDH-B), were immobilized in an array manner by sequent DEP manipulation steps including focusing, guiding and trapping, therefore, two different protein levels can be achieved in single chip without complex microchannel design. The structure of DEP chip consists of three electrode layers, top electrode made of Indium Tin Oxide (ITO), middle Au electrode on SU-8 surface for focusing and guiding of nanoprobes, and bottom ITO dot electrodes under SU-8 cavity for trapping of nanoprobes. In our experiments, two different grade bladder cancer cell lysates (grade I:RT4 and grade III:T24) were introduced into immunosensor, the expression levels of Gal-1 and LDH-B displayed different fluorescent responses for RT4 and T24 cell lysates. Accordingly, the fluorescence intensity of Gal-1 is higher than the value of LDH-B for T24 cell lysate; however, the response for RT4 is opposite, i.e. LDH-B > Gal-1. Consequently, determining of different-grade bladder cancer cell can be achieved by using present multi-antibody immunosensor. In addition, the DEP manipulations of nanoprobes also demonstrate the feasibility for multi-biomarker array in single chip for point-of-care diagnostics.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"39 1","pages":"626-627"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82243982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filling through-silicon vias with conductive ferromagnetic silver-iron composite 用导电铁磁银铁复合材料填充硅通孔

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908828

C. Lu, Kerwin Wang

引用次数: 0

An integrated microfluidic system for screening of peptides specific to colon cancer cells and colon cancer stem cells using the phage display technology 利用噬菌体展示技术筛选结肠癌细胞和结肠癌干细胞特异性肽的集成微流控系统

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908845

Yu-Jui Che, Huei-Wen Wu, Lein-Yu Hung, Hwan-You Chang, Kuan Wang, Gwo-Bin Lee

{"title":"An integrated microfluidic system for screening of peptides specific to colon cancer cells and colon cancer stem cells using the phage display technology","authors":"Yu-Jui Che, Huei-Wen Wu, Lein-Yu Hung, Hwan-You Chang, Kuan Wang, Gwo-Bin Lee","doi":"10.1109/NEMS.2014.6908845","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908845","url":null,"abstract":"Reagents binding specifically to target molecules are essential tools for clinical diagnosis and targeted therapy. Screening of target cell-surface specific affinity reagents with bench-top methods has some drawbacks, including time-consuming, labor-intensive and requirement of large-scale instrument. Microfluidic platforms may overcome these drawbacks because they could automate and complete the screening process within a shorter period of time. Phage display is a promising technology in selection of cell-surface specific peptides. In recent years, a subpopulation of tumor cells named cancer stem cells is believed to be the tumorigenic cells and closely associated with metastasis. A specific peptide that can recognize and differentiate cancer stem cells from the rest of cancer cell population is therefore useful for early diagnosis and targeted therapy. In this study, selection of M13 phage displayed peptides that bind with colon cancer cells and colon cancer stem cells using an integrated microfluidic system was successfully demonstrated. Compared with the traditional methods, the total selection process was shortened to 36 hours while traditional method needs almost a month. More importantly, the screening process can be automated and performed on a single microfluidic chip. The developed technique may be promising for early diagnosis of cancer and target therapeutics.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"52 1","pages":"440-443"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87538907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Super hydrophobic/super hydrophilic transparent nanostructured glass fabricated by wet etching 湿法蚀刻制备超疏水/超亲水透明纳米结构玻璃

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908771

Amarendra Kumar, Kunal Kashyap, K. Liao, M. T. Hou, J. Yeh

引用次数: 0

SOI technology-based microfiltration system for circulating tumor cells isolation and enumeration 基于SOI技术的循环肿瘤细胞分离与计数微滤系统

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908820

Wei Ma, Ronghao Zeng, Dayu Liu, Y. Zohar, Yi-Kuen Lee

引用次数: 2

Experimental investigation of 3D plasmon talbot effect 三维等离子体talbot效应的实验研究

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI: 10.1109/NEMS.2014.6908866

Yiting Yu, H. Zappe

引用次数: 1