Stem Cells International最新文献

{"title":"RETRACTION: Lithium and Copper Induce the Osteogenesis-Angiogenesis Coupling of Bone Marrow Mesenchymal Stem Cells via Crosstalk between Canonical Wnt and HIF-1α Signaling Pathways.","authors":"Stem Cells International","doi":"10.1155/sci/9765145","DOIUrl":"10.1155/sci/9765145","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2021/6662164.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9765145"},"PeriodicalIF":3.6,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Insulin Promotes the Proliferation of Human Umbilical Cord Matrix-Derived Mesenchymal Stem Cells by Activating the Akt-Cyclin D1 Axis\".","authors":"","doi":"10.1155/sci/9879105","DOIUrl":"https://doi.org/10.1155/sci/9879105","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2017/7371615.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9879105"},"PeriodicalIF":3.6,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13389634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair\".","authors":"","doi":"10.1155/sci/9798482","DOIUrl":"https://doi.org/10.1155/sci/9798482","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2019/5738510.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9798482"},"PeriodicalIF":3.6,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13389896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Transcriptomic Analysis Identifies a Key Candidate Gene Bridging Inflammation and DNA Repair in Endometriosis.","authors":"Juan Wang, Wangshu Li, Jiuxiang Feng, Zhongmin Wang","doi":"10.1155/sci/7134066","DOIUrl":"10.1155/sci/7134066","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Endometriosis is a chronic, estrogen-dependent inflammatory disorder characterized by the ectopic implantation of endometrial-like tissue. Although retrograde menstruation is highly prevalent, only a subset of women develops the disease. This epidemiologic paradox suggests that intrinsic molecular alterations in the eutopic endometrium may precondition refluxed cells to survive under inflammatory and oxidative stress.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Eutopic endometrial transcriptomes from the GSE6364 dataset (21 endometriosis patients and 16 controls) were analyzed across menstrual phases. Differential expression analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and hallmark inflammatory gene set profiling were performed. Protein-protein interaction (PPI) networks and three machine-learning algorithms maximal clique centrality (MCC), random forest (RF), and least absolute shrinkage and selection operator (LASSO) were applied to identify robust feature genes. Immune cell infiltration was estimated using CIBERSORT . Excision repair cross-complementation group 1 (ERCC1) was further evaluated via cross-species evolutionary conservation and in silico structural modeling of high-risk variants. Additionally, an exploratory stemness-related single-sample gene set enrichment analysis (ssGSEA) was conducted, and correlations between the stemness score and the feature genes were examined.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 443 differentially expressed genes (DEGs) were identified, which were significantly enriched in inflammatory cascades, including the NF-κB, Toll-like receptor, and cytokine signaling pathways. Four convergent feature genes ERCC1, SOX3, P75NTR (encoded by NGFR), and FPR1 were prioritized. Immune deconvolution revealed selective immune remodeling in the eutopic endometrium, characterized by elevated activated natural killer (NK) cells and reduced CD8&lt;sup&gt;+&lt;/sup&gt; T cells, which correlated significantly with the expression of the feature genes. ERCC1 exhibited high evolutionary conservation, and structural modeling of missense variants predicted the disruption of protein function within conserved DNA repair domains. Exploratory stemness analysis revealed no significant overall case-control difference after adjusting for menstrual phase, though the early secretory subset exhibited a marginally higher score in endometriosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;These findings highlight a stress-adaptive transcriptomic state in the eutopic endometrium driven by inflammatory signaling, selective immune remodeling, and altered DNA repair capacity. Specifically, ERCC1 may serve as a critical mechanistic link between inflammatory pressure and impaired genomic maintenance, thereby facilitating cellular persistence and lesion establishment. Furthermore, our data indicate that this four-gene signature primarily reflects a DNA-repair-adaptive program rather than a global bu","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"7134066"},"PeriodicalIF":3.6,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13386477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal Stem Cell-Derived Exosomes as a Double-Edged Sword: Balancing Inflammation and Immunosuppression in Human Papillomavirus-Infected Tissues.","authors":"Fatemeh Poorhoseini Hanzaii, Masoud Soleimani, Mina SoufiZomorrod","doi":"10.1155/sci/2596453","DOIUrl":"10.1155/sci/2596453","url":null,"abstract":"<p><p>Human papillomavirus (HPV) infection is the most common sexually transmitted viral infection, strongly associated with chronic inflammation and cervical cancer progression in women. Persistent HPV infection leads to an inflammatory microenvironment that promotes epithelial dysplasia and immune evasion. Exosomes derived from mesenchymal stem cells (MSC-exosomes) have emerged as promising immunomodulatory and anti-inflammatory agents. This review examines current evidence on the interaction between HPV-induced inflammation and exosomal signaling, with a particular focus on the therapeutic potential of MSC-exosomes. We discuss their roles in immune regulation, miRNA delivery, suppression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling, and epithelial regeneration. This anti-inflammatory effect may also impair local immune surveillance, potentially enabling viral persistence and progression to malignancy. In this narrative review, We reviewed PubMed, Scopus, and Web of Science articles published up to 2024 on MSC-exosome interactions with immune regulation in HPV-related diseases. Evidence suggests that while the anti-inflammatory effects of MSC-exosomes may help control HPV-associated inflammation, they can also impair local immune surveillance, potentially facilitating viral persistence and progression toward malignancy. This dual activity positions MSC-exosomes as a double-edged sword in the context of HPV pathogenesis. A deeper understanding of this paradox is essential for designing safer, context-specific MSC-based therapies that balance anti-inflammatory benefits with effective antiviral immune responses. Although direct studies on MSC-exosomes in HPV infections are limited, existing models suggest that they have the capacity to attenuate inflammation and restore cervical tissue homeostasis. This article also highlights knowledge gaps and future research directions necessary to develop MSC-exosome-based therapies for HPV-related cervical diseases.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"2596453"},"PeriodicalIF":3.6,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-Scale Production of Secretome From Human Dental Pulp Stem Cells for Articular Cartilage Regeneration.","authors":"Lucía Bravo-Baranda, Lara Milián, María Sancho-Tello, Mauro Llop-Miguel, Irene Monleón-Guinot, Cristina Martínez-Ramos, José Manuel Morales-Tatay, María Fe Mínguez-Rey, Manuel Mata","doi":"10.1155/sci/4200051","DOIUrl":"10.1155/sci/4200051","url":null,"abstract":"<p><strong>Introduction: </strong>Articular cartilage degeneration can lead to a progressive loss of joint function and, eventually, total joint failure. The use of mesenchymal stem cells (MSCs) for cartilage regeneration has proven effective; however, the difficulty of their clinical application has prompted research into other strategies, such as using MSC secretome instead of cells themselves. Our aim was to generate a culture system to obtain large amounts of MSC secretome with a controlled and known composition and the potential to induce articular cartilage regeneration.</p><p><strong>Methods: </strong>Human dental pulp stem cells (hDPSCs) embedded in alginate/agarose beads were cultured in a bioreactor with constant agitation and periodic culture medium exchange. The secretome was collected and characterized according to known chondral markers (TGF-β1, SERPINE1, miR-140, miR-675, miR-23a, miR-204, miR-211, and miR-337-5p). Metabolomic and proteomic analyses were carried out for detailed characterization of the collected secretome. The chondrogenic potential of the collected secretome was tested in an in vitro model developed using human primary chondrocytes cultured in 3D on alginate-agarose scaffolds.</p><p><strong>Results: </strong>Our system allowed us to obtain 1200 mL of secretome from intensive cultures of hDPSCs. The cells cultured on the platform remained viable and in an active anabolic state, secreting large amounts of prochondrogenic mediators. Variability between batches of secretome was low, and the collected secretome induced primary chondrocyte differentiation in vitro.</p><p><strong>Conclusions: </strong>Genomic, metabolomic, and proteomic data indicate that hDPSCs on the platform proliferate and acquire a chondrocyte-like phenotype. These cells secrete mediators that define a microenvironment favorable for chondral regeneration. This is further supported by evidence found in the in vitro differentiation model. Our platform allows the production of large volumes of secretome with controlled composition and chondrogenic induction potential. This is a necessary preclinical study for the subsequent analysis of the secretome obtained using in vivo experimental models.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"4200051"},"PeriodicalIF":3.6,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13379900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Narrative Review of Stem Cell-Derived Exosomes for Diabetic Nephropathy.","authors":"Mo Li, Tianjing Sun, Allen Gao, Andrew Huang, Gagan Deep, Yuanyuan Zhang, Anyong Yu","doi":"10.1155/sci/4021805","DOIUrl":"10.1155/sci/4021805","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) remains a major cause of end-stage kidney disease. Stem cell-derived exosomes have emerged as a promising therapeutic strategy due to their ability to deliver bioactive molecules to damaged tissues. This narrative review, conducted in accordance with PRISMA guidelines, aims to evaluate the therapeutic potential of exosomes derived from various stem cell sources, including mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), in the context of DN. A comprehensive literature search was performed using relevant databases (e.g., PubMed, Web of Science, Scopus) to identify preclinical and clinical studies investigating the effects of stem cell-derived exosomes on DN. The identified studies were assessed for quality and methodological rigor. Priority was given to high-impact studies and those with robust experimental evidence. The selected literature was synthesized thematically to provide a coherent overview of the current state of research on exosome-based therapies for DN, highlighting current findings, and future directions. Results from preclinical studies suggest that exosomes derived from different stem cell sources can exert reno-protective effects, including reducing inflammation, fibrosis, and oxidative stress. However, comparisons between different exosome types indicate that MSCs-derived exosomes (MSC-Exos) may offer superior therapeutic benefits. While clinical trials are ongoing to evaluate the safety and efficacy of stem cell-derived exosomes in DN patients, further research is needed to optimize exosome production, delivery, and therapeutic efficacy.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"4021805"},"PeriodicalIF":3.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13366409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-329-3p in Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Promotes Bone Regeneration in Diabetic Fractures by Alleviating the SIRT3-Mediated Oxidative Stress via Inhibiting LSD1.","authors":"Teng Ma, Mochi Yang, Xiaoli Zhou, Xiaorui Hu, Daihao Wei, Weiwei Guo","doi":"10.1155/sci/6280416","DOIUrl":"10.1155/sci/6280416","url":null,"abstract":"<p><strong>Purpose: </strong>Influence of bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (Exo) on fracture healing in diabetes mellitus (DM) was investigated.</p><p><strong>Methods: </strong>Serum LSD1, SIRT3, superoxide dismutase (SOD), and malondialdehyde (MDA) were detected in patients with diabetic fractures. Under high-glucose (HG) conditions, influences of LSD1, SIRT3, BMSCs-derived Exo, and BMSCs-derived Exo harboring miR-329-3p on osteogenic differentiation and oxidative stress of MC3T3-E1 cells were assayed via ALP staining, western blotting, dichlorofluorescin diacetate (DCFH-DA) staining, and enzyme-linked immunosorbent assay (ELISA). Mice with diabetic fractures were treated by BMSCs, BMSCs-derived Exo, or a combination of BMSCs and GW4869. Western blotting and immunohistochemistry examined protein expression in fracture tissues.</p><p><strong>Results: </strong>DM patients with nonunion showed higher serum LSD1 and MDA and lower serum SIRT3 and SOD than those with normal fracture healing. In HG-induced MC3T3-E1 cells, LSD1 silencing upregulated SIRT3, RUNX2, OPG, and SOD, increased mature osteoblasts, and reduced reactive oxygen species (ROS) and MDA; SIRT3 silencing revered these results. LSD1 downregulated SIRT3, RUNX2, OPG, and SOD, reduced mature osteoblasts, and enhanced ROS and MDA in HG-induced MC3T3-E1 cells; BMSCs-derived Exo abrogated these influences. BMSCs-derived Exo harboring miR-329-3p suppressed LSD1 and ROS, upregulated SIRT3, RUNX2, and OPG, and increased mature osteoblasts in HG-induced MC3T3-E1 cells. BMSCs transplantation in diabetic fracture mice elevated SIRT3, ALP, RUNX2, OPG, and miR-329-3p and reduced LSD1 in fracture tissues; miR-329-3p silencing in BMSCs or GW4869 treatment of mice counteracted these effects.</p><p><strong>Conclusion: </strong>BMSCs-derived Exo harboring miR-329-3p induces bone regeneration in diabetic fractures by relieving the SIRT3-mediated oxidative stress via inhibiting LSD1. It has potential in treating diabetic fractures.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"6280416"},"PeriodicalIF":3.6,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: The Cotransplantation of Olfactory Ensheathing Cells With Bone Marrow Mesenchymal Stem Cells Exerts Antiapoptotic Effects in Adult Rats After Spinal Cord Injury.","authors":"Stem Cells International","doi":"10.1155/sci/9805310","DOIUrl":"10.1155/sci/9805310","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2015/516215.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9805310"},"PeriodicalIF":3.6,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid Hydrogel Inhibits Autophagy Through the miR-181a-5p/ATG5 Molecular Axis to Promote the Adipogenic Differentiation of Adipose-Derived Stem Cells.","authors":"Fengshan Gan, Fan Zheng, Qingzhu Zhou, Boyan Liu, Bin Yang, Lianzhu Ou, Wenli Huang, Xinxin Yang, Zhuo Gong, Yunyu Xiong, Xian Zhao, Jia He","doi":"10.1155/sci/6997518","DOIUrl":"10.1155/sci/6997518","url":null,"abstract":"<p><strong>Background: </strong>Soft tissue defects refer to defects in or damage to human soft tissue, which may affect the appearance, function, and health of the human body and cause physical and psychological distress to the patient. Hyaluronic acid (HA), a fillable material in the field of plastic medicine, could play a crucial role in reconstructing soft tissues and enhancing the adipogenic differentiation of adipose-derived stem cells (ADSCs).</p><p><strong>Methods: </strong>We isolated ADSCs from human subcutaneous adipose tissue and cultured them in HA hydrogel at a density of 2.1 × 10⁶/mL, followed by subcutaneous injections of ADSCs (0.2 mL, 1 × 10⁷ cells/mL with 0.2 mL of DMEM) and HA hydrogel (0.2 mL) into the dorsal side of nude mice. The expression of related genes and proteins was detected using RT‒qPCR and western blotting. The adipogenic differentiation of ADSCs and adipogenesis in nude mice were assessed using CCK-8, Oil Red O, and HE staining.</p><p><strong>Results: </strong>The results revealed an increase in the levels of miR-181a-5p and adipocyte differentiation-related proteins (PPARγ, C/EBPα, FABP4, and adiponectin) and a decrease in the levels of autophagy-related proteins (LC3II/I, Beclin-1, and ATG5) in ADSCs cocultured with HA hydrogel. The adipogenic differentiation ability of ADSCs was enhanced. From a mechanistic standpoint, HA hydrogel inhibited the expression of ATG5 by promoting the expression of miR-181a-5p, thus inhibiting autophagy, promoting the adipogenic differentiation of ADSCs, and promoting the formation of adipose tissue in vivo.</p><p><strong>Conclusions: </strong>Our findings suggest that a combined culture of ADSCs and HA hydrogel may be a new method to enhance the adipogenic differentiation of ADSCs.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"6997518"},"PeriodicalIF":3.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13316802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148362468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}