Stem Cells International最新文献

{"title":"Correction to \"Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFRβ-Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect\".","authors":"","doi":"10.1155/sci/9783731","DOIUrl":"https://doi.org/10.1155/sci/9783731","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2022/2401693.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9783731"},"PeriodicalIF":3.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Evaluation of the Suprachoroidal Space for Stem Cell Delivery in Retinal Degeneration.","authors":"Hyun Hee Seo, Eun Ju Lee, Yoon Young Kim, Seung-Yup Ku, Chang Ki Yoon","doi":"10.1155/sci/1374159","DOIUrl":"https://doi.org/10.1155/sci/1374159","url":null,"abstract":"<p><p>The route of administration is a crucial determinant of the success of stem cell-based therapies, especially in intraocular applications. It significantly influences therapeutic outcomes and the risk of adverse events. We aimed to evaluate the utility of the suprachoroidal space (SCS) as a delivery route for human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) in a rabbit model of retinal degeneration in comparison with the intravitreal (IVT) route. A custom-designed, three-dimensional-printed needle cap was employed to ensure precise hESC-MSC delivery into the SCS via a relatively less invasive approach. Green fluorescent protein (GFP)-induced hESC-MSC expression was monitored for 7 weeks to assess persistence and localization. Therapeutic potential was assessed using electroretinography (ERG) as well as histological and immunofluorescence examinations on Day 28. Flourscence signals were still detectable up to Week 2, which indicated precise delivery to the posterior segment of the eye. There were significant ERG improvements in the hESC-MSC-treated group compared with those in the phosphate-buffered saline-treated group. Furthermore, the hESC-MSC-treated group showed a significant increase in outer nuclear layer thickness and cell number, as well as upregulated rhodopsin and opsin expression. Additionally, there was minimal inflammation in SC-injected eyes compared with that in IVT-injected eyes on Day 14. Our findings highlight the potential of the SCS as an optimal route for hESC-MSC delivery. Specifically, it allows retinal targeting that significantly enhances visual function restoration while minimizing adverse events.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"1374159"},"PeriodicalIF":3.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine-Derived Stem Cells: Applications of Paracrine-Driven Regeneration and Functional Recovery in Multiorgan Systems.","authors":"Dan Li, Zhijuan Liang, Liping Wang, Yuanbin Chen, Dongkai Sun, Ye Liang","doi":"10.1155/sci/8089902","DOIUrl":"https://doi.org/10.1155/sci/8089902","url":null,"abstract":"<p><p>Stem cell therapy, a highly recognized approach in the field of regenerative medicine, is known for its potential to stimulate the self-healing process of the body using stem cells or their derivatives, in which urine-derived stem cells (USCs) stand out as a novel and promising tool in treating various diseases recently. USCs are widely believed to derive primarily from glomerular parietal epithelial cells and podocytes and can be readily isolated from urine. Owing to telomerase-associated self-renewal capacity, USCs proliferate efficiently in vitro, while their outstanding differentiation potential and paracrine effects support promising applications in tissue regeneration and functional recovery. The extracellular particles (EPs) of USCs, including various activity factors, exosomes, and other extracellular vesicles (EVs), play significant roles in the paracrine effects of USCs, which have gained recognition as pivotal factors in the therapeutic studies of USCs. USCs-EPs can affect on diseases in different organ systems through various mechanisms, in which cell differentiation, angiogenesis, and cell proliferation and migration are the three mainly affected functions. And the USCs-EPs may play dual roles based on different curative effects. Besides, the varied modification and encapsulation of USCs-EPs may promote their therapeutic effects. Up to now, USCs-EPs have been widely studied in multiorgan systems and are considered to be superior to USCs because of the rare risks of diverse genetic backgrounds, teratoma formation and immunogenicity. Although in the current preclinical stage, USCs-EPs represent a promising novel tool in stem cell therapy and warrant further exploration for their clinical potential.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"8089902"},"PeriodicalIF":3.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Identification of BMP5 as a Key Regulatory Gene for hPSCs Transcardiomyocyte Differentiation.","authors":"Hong Zhang, Simei Tu, Chang Xu, Ruoshi Wu, Jiayi Wu, Xin Huang, Xiaoqin Luo, Xiaowei Luo, Xinjian Qu","doi":"10.1155/sci/5540587","DOIUrl":"https://doi.org/10.1155/sci/5540587","url":null,"abstract":"<p><strong>Background: </strong>Human pluripotent stem cells (hPSCs)-derived cardiomyocytes have tremendous advantages in generating cell models that are suitable for the study of cardiovascular diseases and their treatment. However, there is a lack of systematic screening and analysis of important genes for inducing hPSCs differentiation into cardiomyocytes.</p><p><strong>Methods: </strong>Gene Expression Omnibus (GEO) databases were used to explore the key genes involved in hPSCs differentiation into cardiomyocytes. Then, the induction of human embryonic stem cells (hESCs) differentiation into cardiomyocytes was verified by alkaline phosphatase (ALP) detection, real-time PCR, western blotting and immunofluorescence experiments. Furthermore, BMP5 overexpression and CRISPER/Cas9-mediated knockout of BMP5 were performed to determine the important role of BMP5 in the differentiation of hESCs into cardiomyocytes.</p><p><strong>Results: </strong>Through differential expression analysis of high-throughput sequencing datasets from four stages of hPSCs differentiation into cardiomyocytes (undifferentiated hPSCs, mesoderm cells, early cardiomyocytes, and cardiomyocytes), 10 key differentially expressed genes (DEGs) were ultimately identified. Weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) network analysis subsequently revealed that BMP5 interacted with the transcriptional regulator NKX2-5. During the induction of hESCs differentiation into cardiomyocytes, the expression of BMP5 was significantly increased in the mesoderm, early cardiomyocyte, and cardiomyocyte stages compared with that in the hESCs stage. After overexpression of BMP5 in hESCs and induction of cell differentiation into cardiomyocytes, the number of myocardial beats and the expression levels of cardiomyocyte marker proteins were significantly greater than those in nontransfected hESCs-derived cardiomyocytes. CRISPR/Cas9-mediated knockout of BMP5 in ESCs and then cells were induced to differentiate towards cardiomyocytes, transcriptome sequencing results revealed a significant reduction in the expression levels of genes related to myocardial development.</p><p><strong>Conclusion: </strong>The role of BMP5 in the differentiation of hESCs into cardiomyocytes was explored, and findings demonstrated that BMP5 promoted the differentiation of hESCs into cardiomyocytes.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"5540587"},"PeriodicalIF":3.3,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes Derived From Mesenchymal Stem Cells Regulating Myotube Cell Atrophy via NF-κB Signaling Pathway.","authors":"Yibing Ke, Yongping Wang","doi":"10.1155/sci/3606738","DOIUrl":"https://doi.org/10.1155/sci/3606738","url":null,"abstract":"<p><p>Bone marrow mesenchymal stem cells (BMSCs), with their potential for multidifferentiation and self-replication, are considered effective for repairing damaged tissues. BMSCs can repair and replace damaged tissues by differentiating into effective cells and secreting some cytokines that inhibit inflammation and promote tissue repair. Recent studies suggest that BMSCs' main role in tissue repair may involve the secretion of exosomes (BMSC-Exos). Basic research has shown that exosomes can have significant effects on orthopedic diseases such as osteoarthritis, muscle tissue injury, and fractures by stimulating regeneration and reducing inflammation. However, the effect of exosomes on tumor necrosis factor (TNF-α)-induced muscle atrophy remains unclear. Therefore, this study investigated the mechanism underlying the protective effect of BMSC-Exos on TNF-α-induced C2C12 myotube atrophy. Treatment with TNF-α (20 ng/mL) for 48 h significantly reduced myotube viability and diameter, which were subsequently reversed by treatment with BMSC-Exos. BMSC-Exos treatment suppressed the expression of E3 ubiquitin ligases, including Atrogin‑1/muscle atrophy F‑box and muscle ring‑finger protein‑1 (MuRF-1). Furthermore, it increased the protein expression levels of myoblast determination protein-1 (MyoD) in TNF-α-induced myotubes. BMSC-Exos also decreased the nuclear translocation of nuclear factor-κB (NF-κB) by inhibiting the phosphorylation of κB inhibitors. These findings indicate that BMSC-Exos may protect against TNF-α-induced myotube atrophy by inhibiting the proinflammatory NF-κB pathway.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"3606738"},"PeriodicalIF":3.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13102087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Administration of Melatonin in Diabetic Retinopathy Is Effective and Improves the Efficacy of Mesenchymal Stem Cell Treatment.","authors":"Stem Cells International","doi":"10.1155/sci/9763520","DOIUrl":"10.1155/sci/9763520","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/6342594.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9763520"},"PeriodicalIF":3.3,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: The Effect of Mesenchymal Stem Cell-Derived Exosomes and miR17-5p Inhibitor on Multicellular Liver Fibrosis Microtissue.","authors":"Stem Cells International","doi":"10.1155/sci/9804587","DOIUrl":"https://doi.org/10.1155/sci/9804587","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2023/8836452.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9804587"},"PeriodicalIF":3.3,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Neurogenic Niche: Interactions Among Vessels, Glia, and Neural Stem Cells.","authors":"Khodakaram Jahanbin","doi":"10.1155/sci/8440764","DOIUrl":"https://doi.org/10.1155/sci/8440764","url":null,"abstract":"<p><p>Adult neurogenesis, the generation of new neurons in the adult brain, acts as a fundamental driver of neural plasticity within specialized microenvironments. The integrity of the hippocampal subgranular zone, essential for pattern separation and mood regulation, relies on a functional syncytium formed by the vasculature, glial cells, and neural stem cells (NSCs). This review delineates the architecture of this system, detailing how the vascular pillar provides angiocrine support via vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor (BDNF), while the glial pillar-comprising astrocytes and microglia-orchestrates metabolic homeostasis and immune surveillance. The dynamic regulation of this local ecosystem by systemic factors, including physical exercise and the gut-brain axis, is also explored. Furthermore, the breakdown of this alliance is examined as a pathological hub in aging, Alzheimer's disease (AD), and chronic stress. Crucially, the text addresses the significant translational gap between rodent models and human physiology. The ongoing controversy regarding the persistence of adult human neurogenesis is critically evaluated, attributing conflicting data to methodological variables such as postmortem interval (PMI) and fixation kinetics. Additionally, the risks of maladaptive plasticity, where aberrant neurogenesis contributes to conditions like epilepsy, are discussed. Finally, future directions involving high-resolution omics and imaging are highlighted, emphasizing that therapeutic strategies must navigate the complex biological risks of neural repair.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"8440764"},"PeriodicalIF":3.3,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-Box Binding Protein 1 Regulates Osteogenesis of Periodontal Ligament Cells During Aging by Modulating P53 Signaling Pathway.","authors":"Youli Zheng, Mengru Zhai, Xijing Bai, Kaiqi Yu, Jia Li, Zheng Zhang","doi":"10.1155/sci/2846096","DOIUrl":"https://doi.org/10.1155/sci/2846096","url":null,"abstract":"<p><strong>Background and objective: </strong>Aging impairs the osteogenic differentiation of periodontal ligament stem cells (PDLSCs), but the mechanism is unclear. X-box binding protein 1 (XBP1), a transcription factor affecting cell senescence, may be involved. This study focuses on the role and mechanism of XBP1 in PDLSCs' osteogenic differentiation during aging.</p><p><strong>Methods: </strong>Young PDLSCs (Y-PDLSCs) and elderly PDLSCs (E-PDLSCs) were obtained. XBP1 expression was quantified using both real-time polymerase chain reaction (RT-PCR) and western blotting (WB). Lentiviral infection was employed to modulate XBP1 expression, with pLVX-XBP1 utilized for upregulation and shXBP1 for downregulation. The degree of senescence in PDLSCs was determined using β-galactosidase staining and cell cycle analysis. PDLSCs' osteogenic differentiation was assessed through WB, alkaline phosphatase (ALP), and alizarin red staining. The Gene Transcription Regulation Database (GTRD) and microarray analysis was used to identify XBP1's target genes and potential pathways. Additionally, PFTα was applied to inhibit the P53/P21 pathway in PDLSCs.</p><p><strong>Results: </strong>E-PDLSCs had higher β-galactosidase expression, suppressed cell cycle, and decreased osteogenic differentiation compared to Y-PDLSCs. XBP1 expression in PDLSCs decreased with aging, and upregulation of XBP1 by pLVX-XBP1 partially reversed this aging process. Osteogenic induction increased XBP1 expression in PDLSCs, and pLVX-XBP1 facilitated the osteogenic differentiation of these cells. The target genes of XBP1 were enriched in the P53/P21 pathway, which was highly expressed in P9 PDLSCs but showed lower expression following XBP1 overexpression. Moreover, inhibiting the P53/P21 pathway by PFTα could partially reverse the inhibitory effect of shXBP1 on PDLSCs' osteogenic differentiation.</p><p><strong>Conclusions: </strong>XBP1 plays a pivotal role in the regulation of PDLSCs' osteogenic differentiation during the aging process, at least in part, through the modulation of the P53/P21 pathway.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"2846096"},"PeriodicalIF":3.3,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13067754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Scaffold-Based 3D Culture Model Including Selected Osteoprogenitors for Bone Regeneration With Controlled Morphology.","authors":"Thi Hue Mai, Shousaku Itoh, Takumi Kagioka, Makato Abe, Mikako Hayashi","doi":"10.1155/sci/1860064","DOIUrl":"https://doi.org/10.1155/sci/1860064","url":null,"abstract":"<p><p>We previously identified \"highly purified osteoprogenitors\" (HipOPs) as a promising model for studying bone organ regeneration in murine systems. However, achieving proper integration of the bone formed by HipOPs into the well-organized host bone requires better morphological control. In this study, we developed a 3D culture model by culturing a HipOP-seeded gelatin sponge on an osteoblast (OB) layer to regulate the morphology of regenerated bone. In vitro studies revealed significant upregulation of genes associated with bone formation, response to stimuli, cell communication, and vascularization in the HipOP population after 48 h of coculture. Moreover, the HipOPs exhibited concentration-dependent responses, with stronger effects observed in regions closer to the OB layer. By day 7, the sponge areas in contact with the OB layer contained more cells with enhanced osteogenic differentiation capacity. When transplanted subcutaneously into immunodeficient mice for 8 weeks, the 3D culture samples formed bone organs comprising bone and bone marrow tissue. Notably, cortical bone appeared only in regions where the sponge had contacted the OB layer, with a gradual decrease in bone density observed outward from that area. Our 3D culture model successfully generated hierarchically structured bone organs, offering significant potential for reconstructing well-integrated bone in defective regions.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"1860064"},"PeriodicalIF":3.3,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13066912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}