Stem Cells International最新文献

{"title":"Large-Scale Production of Secretome From Human Dental Pulp Stem Cells for Articular Cartilage Regeneration.","authors":"Lucía Bravo-Baranda, Lara Milián, María Sancho-Tello, Mauro Llop-Miguel, Irene Monleón-Guinot, Cristina Martínez-Ramos, José Manuel Morales-Tatay, María Fe Mínguez-Rey, Manuel Mata","doi":"10.1155/sci/4200051","DOIUrl":"10.1155/sci/4200051","url":null,"abstract":"<p><strong>Introduction: </strong>Articular cartilage degeneration can lead to a progressive loss of joint function and, eventually, total joint failure. The use of mesenchymal stem cells (MSCs) for cartilage regeneration has proven effective; however, the difficulty of their clinical application has prompted research into other strategies, such as using MSC secretome instead of cells themselves. Our aim was to generate a culture system to obtain large amounts of MSC secretome with a controlled and known composition and the potential to induce articular cartilage regeneration.</p><p><strong>Methods: </strong>Human dental pulp stem cells (hDPSCs) embedded in alginate/agarose beads were cultured in a bioreactor with constant agitation and periodic culture medium exchange. The secretome was collected and characterized according to known chondral markers (TGF-β1, SERPINE1, miR-140, miR-675, miR-23a, miR-204, miR-211, and miR-337-5p). Metabolomic and proteomic analyses were carried out for detailed characterization of the collected secretome. The chondrogenic potential of the collected secretome was tested in an in vitro model developed using human primary chondrocytes cultured in 3D on alginate-agarose scaffolds.</p><p><strong>Results: </strong>Our system allowed us to obtain 1200 mL of secretome from intensive cultures of hDPSCs. The cells cultured on the platform remained viable and in an active anabolic state, secreting large amounts of prochondrogenic mediators. Variability between batches of secretome was low, and the collected secretome induced primary chondrocyte differentiation in vitro.</p><p><strong>Conclusions: </strong>Genomic, metabolomic, and proteomic data indicate that hDPSCs on the platform proliferate and acquire a chondrocyte-like phenotype. These cells secrete mediators that define a microenvironment favorable for chondral regeneration. This is further supported by evidence found in the in vitro differentiation model. Our platform allows the production of large volumes of secretome with controlled composition and chondrogenic induction potential. This is a necessary preclinical study for the subsequent analysis of the secretome obtained using in vivo experimental models.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"4200051"},"PeriodicalIF":3.6,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13379900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Narrative Review of Stem Cell-Derived Exosomes for Diabetic Nephropathy.","authors":"Mo Li, Tianjing Sun, Allen Gao, Andrew Huang, Gagan Deep, Yuanyuan Zhang, Anyong Yu","doi":"10.1155/sci/4021805","DOIUrl":"10.1155/sci/4021805","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) remains a major cause of end-stage kidney disease. Stem cell-derived exosomes have emerged as a promising therapeutic strategy due to their ability to deliver bioactive molecules to damaged tissues. This narrative review, conducted in accordance with PRISMA guidelines, aims to evaluate the therapeutic potential of exosomes derived from various stem cell sources, including mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), in the context of DN. A comprehensive literature search was performed using relevant databases (e.g., PubMed, Web of Science, Scopus) to identify preclinical and clinical studies investigating the effects of stem cell-derived exosomes on DN. The identified studies were assessed for quality and methodological rigor. Priority was given to high-impact studies and those with robust experimental evidence. The selected literature was synthesized thematically to provide a coherent overview of the current state of research on exosome-based therapies for DN, highlighting current findings, and future directions. Results from preclinical studies suggest that exosomes derived from different stem cell sources can exert reno-protective effects, including reducing inflammation, fibrosis, and oxidative stress. However, comparisons between different exosome types indicate that MSCs-derived exosomes (MSC-Exos) may offer superior therapeutic benefits. While clinical trials are ongoing to evaluate the safety and efficacy of stem cell-derived exosomes in DN patients, further research is needed to optimize exosome production, delivery, and therapeutic efficacy.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"4021805"},"PeriodicalIF":3.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13366409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-329-3p in Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Promotes Bone Regeneration in Diabetic Fractures by Alleviating the SIRT3-Mediated Oxidative Stress via Inhibiting LSD1.","authors":"Teng Ma, Mochi Yang, Xiaoli Zhou, Xiaorui Hu, Daihao Wei, Weiwei Guo","doi":"10.1155/sci/6280416","DOIUrl":"10.1155/sci/6280416","url":null,"abstract":"<p><strong>Purpose: </strong>Influence of bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (Exo) on fracture healing in diabetes mellitus (DM) was investigated.</p><p><strong>Methods: </strong>Serum LSD1, SIRT3, superoxide dismutase (SOD), and malondialdehyde (MDA) were detected in patients with diabetic fractures. Under high-glucose (HG) conditions, influences of LSD1, SIRT3, BMSCs-derived Exo, and BMSCs-derived Exo harboring miR-329-3p on osteogenic differentiation and oxidative stress of MC3T3-E1 cells were assayed via ALP staining, western blotting, dichlorofluorescin diacetate (DCFH-DA) staining, and enzyme-linked immunosorbent assay (ELISA). Mice with diabetic fractures were treated by BMSCs, BMSCs-derived Exo, or a combination of BMSCs and GW4869. Western blotting and immunohistochemistry examined protein expression in fracture tissues.</p><p><strong>Results: </strong>DM patients with nonunion showed higher serum LSD1 and MDA and lower serum SIRT3 and SOD than those with normal fracture healing. In HG-induced MC3T3-E1 cells, LSD1 silencing upregulated SIRT3, RUNX2, OPG, and SOD, increased mature osteoblasts, and reduced reactive oxygen species (ROS) and MDA; SIRT3 silencing revered these results. LSD1 downregulated SIRT3, RUNX2, OPG, and SOD, reduced mature osteoblasts, and enhanced ROS and MDA in HG-induced MC3T3-E1 cells; BMSCs-derived Exo abrogated these influences. BMSCs-derived Exo harboring miR-329-3p suppressed LSD1 and ROS, upregulated SIRT3, RUNX2, and OPG, and increased mature osteoblasts in HG-induced MC3T3-E1 cells. BMSCs transplantation in diabetic fracture mice elevated SIRT3, ALP, RUNX2, OPG, and miR-329-3p and reduced LSD1 in fracture tissues; miR-329-3p silencing in BMSCs or GW4869 treatment of mice counteracted these effects.</p><p><strong>Conclusion: </strong>BMSCs-derived Exo harboring miR-329-3p induces bone regeneration in diabetic fractures by relieving the SIRT3-mediated oxidative stress via inhibiting LSD1. It has potential in treating diabetic fractures.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"6280416"},"PeriodicalIF":3.6,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: The Cotransplantation of Olfactory Ensheathing Cells With Bone Marrow Mesenchymal Stem Cells Exerts Antiapoptotic Effects in Adult Rats After Spinal Cord Injury.","authors":"Stem Cells International","doi":"10.1155/sci/9805310","DOIUrl":"10.1155/sci/9805310","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2015/516215.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9805310"},"PeriodicalIF":3.6,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid Hydrogel Inhibits Autophagy Through the miR-181a-5p/ATG5 Molecular Axis to Promote the Adipogenic Differentiation of Adipose-Derived Stem Cells.","authors":"Fengshan Gan, Fan Zheng, Qingzhu Zhou, Boyan Liu, Bin Yang, Lianzhu Ou, Wenli Huang, Xinxin Yang, Zhuo Gong, Yunyu Xiong, Xian Zhao, Jia He","doi":"10.1155/sci/6997518","DOIUrl":"10.1155/sci/6997518","url":null,"abstract":"<p><strong>Background: </strong>Soft tissue defects refer to defects in or damage to human soft tissue, which may affect the appearance, function, and health of the human body and cause physical and psychological distress to the patient. Hyaluronic acid (HA), a fillable material in the field of plastic medicine, could play a crucial role in reconstructing soft tissues and enhancing the adipogenic differentiation of adipose-derived stem cells (ADSCs).</p><p><strong>Methods: </strong>We isolated ADSCs from human subcutaneous adipose tissue and cultured them in HA hydrogel at a density of 2.1 × 10⁶/mL, followed by subcutaneous injections of ADSCs (0.2 mL, 1 × 10⁷ cells/mL with 0.2 mL of DMEM) and HA hydrogel (0.2 mL) into the dorsal side of nude mice. The expression of related genes and proteins was detected using RT‒qPCR and western blotting. The adipogenic differentiation of ADSCs and adipogenesis in nude mice were assessed using CCK-8, Oil Red O, and HE staining.</p><p><strong>Results: </strong>The results revealed an increase in the levels of miR-181a-5p and adipocyte differentiation-related proteins (PPARγ, C/EBPα, FABP4, and adiponectin) and a decrease in the levels of autophagy-related proteins (LC3II/I, Beclin-1, and ATG5) in ADSCs cocultured with HA hydrogel. The adipogenic differentiation ability of ADSCs was enhanced. From a mechanistic standpoint, HA hydrogel inhibited the expression of ATG5 by promoting the expression of miR-181a-5p, thus inhibiting autophagy, promoting the adipogenic differentiation of ADSCs, and promoting the formation of adipose tissue in vivo.</p><p><strong>Conclusions: </strong>Our findings suggest that a combined culture of ADSCs and HA hydrogel may be a new method to enhance the adipogenic differentiation of ADSCs.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"6997518"},"PeriodicalIF":3.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13316802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148362468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Evaluation of Mechanical Fat Processing Devices: Impact on Adipocytes and Adipose-Derived Stem Cells.","authors":"Sheila Veronese, Riccardo Ossanna, Sara Ghazanfar Tehrani, Lorena Torroni, Patricija Kasilovska, Mario Goisis, Giamaica Conti, Domenico Amuso, Antonio Scarano, Andrea Sbarbati","doi":"10.1155/sci/9150101","DOIUrl":"10.1155/sci/9150101","url":null,"abstract":"<p><p>In the fat grafting procedure, the new European Regulations and the necessity to perform minimal manipulation processing require the verification of security and performance levels of each device and, in this particular context, functional and tissue responses. The aim of this study is to evaluate the integrity of adipocytes and adipose-derived stromal/stem cells (ADSCs) after their processing using two new mechanical devices operating with a closed system. In this study, two new fat processing systems were assessed. We also considered the biological results of the processing and compared them with the results obtained from Coleman's procedure, the golden standard procedure in fat processing. Histological evaluations revealed the preservation of the fat morphology after both processing types, with similar cellular yields of the extracted ADSCs. The adopted techniques enable the isolation of ADSCs with robust differentiation potential toward adipogenic, chondrogenic, and osteogenic lineages. Furthermore, adipose tissue samples exhibit high efficiency in extracellular vesicle secretion, indicating a promising potential for therapeutic and regenerative applications. The results suggest that the new systems allow the preservation of fat morphology, stemness, and regenerative potential. The novel processing technique proposed by the authors consists of a closed-loop mechanical system that combines continuous filtration and emulsification of adipose tissue through saline washing, aimed at removing biological debris and residual pharmacological agents.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9150101"},"PeriodicalIF":3.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13319391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148369697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Naringin Stimulates Osteogenic Differentiation of Rat Bone Marrow Stromal Cells via Activation of the Notch Signaling Pathway.","authors":"Stem Cells International","doi":"10.1155/sci/9852061","DOIUrl":"10.1155/sci/9852061","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2016/7130653.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"9852061"},"PeriodicalIF":3.6,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13307187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Derivation and Transcriptional Characterization of Mouse Extra-Embryonic Endoderm Stem Cell Lines Generated by Somatic Cell Nuclear Transfer.","authors":"Shuaipeng Li, Shu Wei, Guomeng Li, Mei Hu, Jiangwei Lin, Wandong Bao","doi":"10.1155/sci/1968738","DOIUrl":"10.1155/sci/1968738","url":null,"abstract":"<p><p>Somatic cell nuclear transfer (SCNT) holds great promise for regenerative medicine and agriculture, but its application is severely hampered by low efficiency, primarily attributable to aberrant epigenetic reprogramming. Although embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) have been successfully derived from cloned embryos, an in vitro counterpart of the primitive endoderm (PrE) lineage has remained unavailable. To address this gap, this study reports the first successful establishment of extra-embryonic endoderm stem cell lines (XENs) from mouse SCNT-derived blastocysts (NT-XENs). Under conventional culture conditions, NT-XENs were generated from hybrid B6D2F1 blastocysts at a high efficiency of 55%, statistically comparable to that of fertilization-derived XEN lines (FD-XENs, 50%), whereas derivation from inbred C57BL/6J SCNT-derived blastocysts was markedly lower (12.5%). Immunofluorescence and NanoString multiplex gene expression profiling confirmed that NT-XENs robustly expressed specific marker genes for PrE/XENs (e.g., <i>Gata4</i>, <i>Gata6</i>, and <i>Sox17</i>), while exhibiting negligible or absent expression of pluripotency and trophoblast markers. Based on NanoString assay data, NT-XENs and FD-XENs shared highly similar gene expression patterns, yet also exhibited some nonnegligible differences, exemplified by the differentially expressed genes (DEGs) <i>Pecam1</i>, <i>Gtl2</i>, <i>Thbd</i>, and <i>Xlr3b</i>. These differences raise a preliminary hypothesis that the NT-XENs might exhibit a slight transcriptional propensity toward a more differentiated state, and potentially reflect lingering traces of SCNT-associated epigenetic errors, such as localized dysregulation of imprinted genes and X-linked transcripts. In summary, this study successfully establishes NT-XEN cell lines, providing a valuable in vitro model for investigating the reprogramming scenarios of PrE lineage in SCNT and the mechanisms underlying developmental failure of cloned embryos.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"1968738"},"PeriodicalIF":3.6,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13291796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148333274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotection After Intracerebral Hemorrhage: Apoptosis, Neurogenesis, and Prospects of Stem Cell Therapy.","authors":"Ziwei Wang, Jing Tian, Ying Kong","doi":"10.1155/sci/4696038","DOIUrl":"10.1155/sci/4696038","url":null,"abstract":"<p><p>This comprehensive review examines the interconnected roles of apoptosis, neurogenesis, and stem cell therapy in neuroprotection and neurorestoration following intracerebral hemorrhage (ICH). We emphasize that these processes should be interpreted within a unified secondary injury cascade rather than as independent topics. Following ICH, hematoma toxicity, erythrocyte lysis, iron overload, oxidative stress, inflammation, blood-brain barrier disruption, and perihematomal edema interact to activate multiple regulated cell death pathways. Apoptosis remains important, but ferroptosis and other inflammatory cell-death programs are also increasingly relevant to ICH research. The endogenous neurogenic response following ICH is analyzed, highlighting both its potential and its marked limitations in spontaneous recovery, particularly the poor survival and functional integration of newborn cells. We then examine how stem cell therapy may bridge antiapoptotic neuroprotection and proneurogenic neurorestoration through paracrine signaling, microenvironment modulation, and limited cell replacement. Current diagnostic advances and clinical trial designs for stem cell therapy in ICH are reviewed, but we also discuss major translational barriers, including weak distinction between rodent and human evidence, limited cell survival, delivery-route constraints, immune compatibility, source heterogeneity, and tumorigenicity. Finally, we propose that future progress will require stage-specific and clinically translatable strategies rather than isolated pathway targeting. This integrated framework may help refine therapeutic priorities for ICH patients.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"4696038"},"PeriodicalIF":3.6,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13280664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148296204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amentoflavone Suppresses Stemness of Retinoblastoma Cell via Targeting Smoothened (SMO) Protein.","authors":"Cuiping Li, Xin Yu, Xiangfen Kong, Qian Zheng, Yu Pan, Dengmei Qi","doi":"10.1155/sci/7261941","DOIUrl":"10.1155/sci/7261941","url":null,"abstract":"<p><p>Retinoblastoma (RB), a malignant intraocular tumor, represents a critical public health challenge, particularly for pediatric populations, underscoring the urgent need for innovative therapeutic approaches and novel drug development. In this study, amentoflavone (AMF) was identified as a potential agent against RB cancer stem cells (RBCSCs). While CCK8 assays revealed moderate anti-proliferative effects of AMF on RB cell lines, migration and invasion assays demonstrated its potent ability to suppress cancer cell motility. Tumorsphere formation assays further indicated that AMF significantly reduces RB cell stemness. qRT-PCR analysis showed that AMF downregulates expression of stem cell markers (CD44, CD133, Oct4, and Nanog) in a dose-dependent manner. In vivo studies confirmed that AMF inhibits tumor metastasis and prolongs survival in RB mouse models. To elucidate its mechanism of action, RNA sequencing, molecular docking, and surface plasmon resonance (SPR) were employed. These analyses revealed that AMF directly targets smoothened (SMO) to disrupt the SHh signaling pathway, thereby suppressing RB stem cell (RBSC) self-renewal and tumor progression. This work uncovers a previously unreported mechanism by which AMF hinders RB development and highlights its potential as a promising therapeutic candidate for this aggressive pediatric cancer.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2026 ","pages":"7261941"},"PeriodicalIF":3.6,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}