Stem Cells International最新文献_第5页

The Neural Progenitor Cell-Associated Transcription Factor FoxG1 Regulates Cardiac Epicardial Cell Proliferation 神经祖细胞相关转录因子 FoxG1 调控心脏外膜细胞增殖

IF 4.3 3区医学

Stem Cells International Pub Date : 2024-01-11 DOI: 10.1155/2024/8601360

Lucy Pilcher, Lara Solomon, Julie A. Dragon, Dhananjay Gupta, Jeffrey L. Spees

{"title":"The Neural Progenitor Cell-Associated Transcription Factor FoxG1 Regulates Cardiac Epicardial Cell Proliferation","authors":"Lucy Pilcher, Lara Solomon, Julie A. Dragon, Dhananjay Gupta, Jeffrey L. Spees","doi":"10.1155/2024/8601360","DOIUrl":"https://doi.org/10.1155/2024/8601360","url":null,"abstract":"The epicardium is a layer of mesothelial cells that covers the surface of the heart. During development, epicardial cells undergo epithelial-to-mesenchymal transition (EMT) to form multipotent precursors that migrate into the heart and contribute to the coronary vasculature by differentiating into adventitial fibroblasts, smooth muscle cells, and endothelial cells. Epicardial cells also provide paracrine signals to cardiac myocytes that are required for appropriate heart growth. In adult hearts, a similar process of epicardial cell EMT, migration, and differentiation occurs after myocardial infarction (MI, heart attack). Pathological cardiac hypertrophy is associated with fibrosis, negative remodeling, and reduced cardiac function. In contrast, aerobic exercises such as swimming and running promote physiological (i.e., beneficial) hypertrophy, which is associated with angiogenesis and improved cardiac function. As epicardial cell function(s) during physiological hypertrophy are poorly understood, we analyzed and compared the native epicardial cells isolated directly from the hearts of running-exercised mice and age-matched, nonrunning littermates. To obtain epicardial cells, we enzymatically digested the surfaces of whole hearts and performed magnetic-activated cell sorting (MACS) with antibodies against CD104 (integrin β4). By cDNA microarray assays, we identified genes with increased transcription in epicardial cells after running exercise; these included FoxG1, a transcription factor that controls neural progenitor cell proliferation during brain development and Snord116, a small noncoding RNA that coordinates expression of genes with epigenetic, circadian, and metabolic functions. In cultured epicardial cells, shRNA-mediated FoxG1 knockdown significantly decreased cell proliferation, as well as Snord116 expression. Our results demonstrate that FoxG1 regulates epicardial proliferation, and suggest it may affect cardiac remodeling.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"85 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139421806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an m6A-Related lncRNAs Signature Predicts Tumor Stemness and Prognosis for Low-Grade Glioma Patients 开发与 m6A 相关的 lncRNAs Signature 预测低级别胶质瘤患者的肿瘤干性和预后

IF 4.3 3区医学

Stem Cells International Pub Date : 2024-01-09 DOI: 10.1155/2024/2062283

Dahua Xu, Peihu Li, Chunrui Zhang, Yutong Shen, Jiale Cai, Qingchen Wei, Meng Cao, Zhizhou Xu, Deng Wu, Hong Wang, Xiaoman Bi, Bo Wang, Kongning Li

{"title":"Development of an m6A-Related lncRNAs Signature Predicts Tumor Stemness and Prognosis for Low-Grade Glioma Patients","authors":"Dahua Xu, Peihu Li, Chunrui Zhang, Yutong Shen, Jiale Cai, Qingchen Wei, Meng Cao, Zhizhou Xu, Deng Wu, Hong Wang, Xiaoman Bi, Bo Wang, Kongning Li","doi":"10.1155/2024/2062283","DOIUrl":"https://doi.org/10.1155/2024/2062283","url":null,"abstract":"Background. Growing evidence has revealed that m6A modification of long noncoding RNAs (lncRNAs) dynamically controls tumor stemness and tumorigenesis-related processes. However, the prognostic significance of m6A-related lncRNAs and their associations with stemness in low-grade glioma (LGG) remain to be clarified. Methods. A multicenter transcriptome analysis of lncRNA expression in 1,247 LGG samples was performed in this study. The stemness landscape of LGG tumors was presented and associations with clinical features were revealed. The m6A-related lncRNAs were identified between stemness groups and were further prioritized via least absolute shrinkage and selection operator Cox regression analysis. A risk score model based on m6A-related lncRNAs was constructed and validated in external LGG datasets. Results. Based on the expression of LINC02984, PFKP-DT, and CRNDE, a risk model and nomogram were constructed; they successfully predicted the survival of patients and were extended to external datasets. Significant correlations were observed between the risk score and tumor stemness. Moreover, patients in different risk groups exhibited distinct tumor immune microenvironments and immune signatures. We finally provided several potential compounds suitable for specific risk groups, which may aid in LGG treatment. Conclusions. This novel signature presents noteworthy value in the prediction of prognosis and stemness status for LGG patients and will foster future research on the development of clinical regimens.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"9 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139408522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Angiogenesis and Its Biomarkers in Development of Targeted Tumor Therapies 血管生成及其生物标志物在开发肿瘤靶向治疗中的作用

IF 4.3 3区医学

Stem Cells International Pub Date : 2024-01-04 DOI: 10.1155/2024/9077926

Anchal Pathak, Ajay Kumar Pal, Subhadeep Roy, Mukesh Nandave, Keerti Jain

引用次数: 0

Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid-Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis 间充质干细胞通过调节 Ekt/Akt/TRIM29 轴加速醋酸诱导的慢性胃溃疡的恢复

IF 4.3 3区医学

Stem Cells International Pub Date : 2024-01-03 DOI: 10.1155/2024/6202123

Feiyue Zhao, Zhibin Fan, Ruikang Jia, Qichao Liu, Menglei Wang, Jianliang Sui, Huiyun Liu

{"title":"Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid-Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis","authors":"Feiyue Zhao, Zhibin Fan, Ruikang Jia, Qichao Liu, Menglei Wang, Jianliang Sui, Huiyun Liu","doi":"10.1155/2024/6202123","DOIUrl":"https://doi.org/10.1155/2024/6202123","url":null,"abstract":"Chronic gastric ulcer (CGU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Mesenchymal stem cells (MSCs) have been proven to have beneficial therapeutic effects in many human diseases. Here, a CGU model induced by acetic acid in mice was used to evaluate the repair effects and potential mechanism of human umbilical cord-derived MSCs (hUC-MSCs) and hUC-MSCs derived conditioned medium (hUC-MSC-CM). We found that hUC-MSCs and hUC-MSC-CM treatment significantly repaired morphological characteristics of CGU, improved proliferation and decreased apoptosis of gastric cells, and promoted the generation of new blood vessels in granulation tissues. In addition, we could detect the homing of MSCs in gastric tissue, and MSCs may differentiate into Lgr5-positive cells. As well as this, in vitro experiments showed that hUC-MSC-CM could promote cell proliferation, stimulate cell cycle progression, and reduce the incidence of apoptosis. The transcriptome of cells and the iTRAQ proteome of gastric tissues suggest that MSCs may play a therapeutic role by increasing the expression of TRIM29. Additionally, it was found that knocking down TRIM29 significantly decreased the ameliorative effects of hUC-MSC-CM on cell apoptosis. As a result of further molecular experiments, it was found that TRIM29 is capable of phosphorylating Erk/Akt in specific cell type. As a whole, it appears that hUC-MSCs can be an effective therapeutic approach for promoting gastric ulcer healing and may exert therapeutic effects in the form of paracrine and differentiation into gastric cells.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"207 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139082617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose Tissue-Derived Extracellular Vesicles: A Promising Biomarker and Therapeutic Strategy for Metabolic Disorders 脂肪组织衍生的细胞外囊泡：代谢紊乱的有望生物标记物和治疗策略

IF 4.3 3区医学

Stem Cells International Pub Date : 2023-12-26 DOI: 10.1155/2023/9517826

Wenhui Liu, Tianyan Liu, Qingyu Zhao, Junqiu Ma, Jiajia Jiang, Hui Shi

引用次数: 0

Retracted: Scutellarin Loaded on Ultradeformable Nanoliposome Scutellarin EDTMP (S-UNL-E) Promotes Osteogenesis in Osteoporotic Rats 撤回：负载在超可塑纳米脂质体黄芩苷 EDTMP（S-UNL-E）上的黄芩苷促进骨质疏松大鼠的骨生成

IF 4.3 3区医学

Stem Cells International Pub Date : 2023-12-20 DOI: 10.1155/2023/9851693

Stem Cells International

引用次数: 0

Retracted: Exploring the Mechanism of Wenshen Huatan Quyu Decotion for PCOS Based on Network Pharmacology and Molecular Docking Verification. 撤回：基于网络药理学和分子对接验证的温神化瘀解毒丸治疗多囊卵巢综合征的机制探索