Stem Cells International最新文献_第3页

Effects and Mechanisms of Extracellular Vesicles in Different Models of Acute Kidney Injury. 细胞外囊泡在不同急性肾损伤模型中的作用及机制。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1155/sci/1075016

Weidong Wang, Jingyu Wang, Dan Liao

{"title":"Effects and Mechanisms of Extracellular Vesicles in Different Models of Acute Kidney Injury.","authors":"Weidong Wang, Jingyu Wang, Dan Liao","doi":"10.1155/sci/1075016","DOIUrl":"10.1155/sci/1075016","url":null,"abstract":"Acute kidney injury (AKI) is a rapid decline in renal function caused by ischemia/reperfusion (I/R), renal toxic injury, and sepsis. While the precise molecular mechanisms underlying AKI are still under investigation, current therapeutic approaches remain insufficient. In recent years, there has been growing evidence that mesenchymal stem cells (MSCs) have great potential in accelerating renal repair after AKI in various preclinical models, while there has been extensive research on extracellular vesicles (EVs) as therapeutic mediators in AKI models, and they are considered to be superior to MSCs as new regenerative therapies. EVs are nanoparticles secreted by various types of cells under physiological and pathological conditions. EVs derived from various sources possess biomarker potential and play crucial roles in mediating cellular communication between kidney cells and other tissue cells by transmitting signal molecules. These vesicles play a direct and indirect role in regulating the pathophysiological mechanisms of AKI and contribute to the occurrence, development, treatment, and repair of AKI. In this review, we briefly outline the essential characteristics of EVs, focus on the multiple molecular mechanisms currently involved in the protection of EVs against different types of AKI, and further discuss the potential targets of EVs from different sources in the treatment of AKI. Finally, we summarized the deficiencies in the production and treatment of EVs and the current strategies for improvement.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"1075016"},"PeriodicalIF":3.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review: Advances in Mesenchymal Stem Cell Applications for Burn Wound Repair. 间充质干细胞在烧伤创面修复中的应用进展

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI: 10.1155/sci/6683745

Hui-Juan Zhang, Jing-Jie Ming, Hong-Xiao Zhang, Shao-Yi-Han Fang, Quan-Wen Liu, Hong-Yan Zhang

{"title":"A Comprehensive Review: Advances in Mesenchymal Stem Cell Applications for Burn Wound Repair.","authors":"Hui-Juan Zhang, Jing-Jie Ming, Hong-Xiao Zhang, Shao-Yi-Han Fang, Quan-Wen Liu, Hong-Yan Zhang","doi":"10.1155/sci/6683745","DOIUrl":"10.1155/sci/6683745","url":null,"abstract":"Tissue repair following skin injury is a complex process that encompasses hemostasis, inflammation, tissue cell proliferation, and structural remodeling. Mesenchymal stem cells (MSCs) are derived from the mesodermal layer of tissues and possess multidirectional differentiation potential and self-renewal capabilities. MSCs from various sources, including the bone marrow, adipose tissue, dental pulp, umbilical cord, and amniotic membrane, have demonstrated effectiveness in promoting skin injury repair. They aid in this process by fostering the formation of new blood vessels in damaged tissues, self-renewal, or transdifferentiation into skin or sweat gland cells. Moreover, MSCs promote the proliferation and migration of skin cells, reduce wound inflammation, and restore the extracellular matrix through paracrine secretion. In this paper, we review recent findings regarding MSCs and their role in burn wound repair. Additionally, we explore the potential of combining MSCs with various biomaterials for treating burn wounds and analyze clinical cases wherein MSCs were administered to patients, offering insights into ongoing research on MSC-based therapies for skin injuries.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"6683745"},"PeriodicalIF":3.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of TP Rat Pancreatic Acinar Cell Apoptosis by hucMSC-Ex Carrying hsa-miR-21-5p via PTEN/PI3K Regulation. 携带hsa-miR-21-5p的hucscs - ex通过PTEN/PI3K调控抑制TP大鼠胰腺腺泡细胞凋亡

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI: 10.1155/sci/8883585

Zhao Zhirong, Jiang Kexin, Yuan Mu, Zhou Lichen, Tan Zhen, Liang Hongyin, Dai Ruiwu

{"title":"Suppression of TP Rat Pancreatic Acinar Cell Apoptosis by hucMSC-Ex Carrying hsa-miR-21-5p via PTEN/PI3K Regulation.","authors":"Zhao Zhirong, Jiang Kexin, Yuan Mu, Zhou Lichen, Tan Zhen, Liang Hongyin, Dai Ruiwu","doi":"10.1155/sci/8883585","DOIUrl":"10.1155/sci/8883585","url":null,"abstract":"Objective: The traumatic pancreatitis (TP) has an alarmingly high mortality rate. Our previous research has demonstrated that human umbilical cord mesenchymal stem cells-derived exosomes (hucMSC-Exs) could treat TP by inhibiting acinar cell apoptosis. Accordingly, the objective of this study is to unravel the intricate mechanism behind the repair of pancreatic injury in TP rats. Methods: A gene interaction network of miRNA was constructed based on the Gene Expression Omnibus (GEO) database (GSE 159814). Our investigation was divided into two groups, and appropriate controls were implemented for each group. The expression levels of inflammatory factors in each group were detected, along with the pathological damage of pancreatic tissue, the percentage of apoptotic cells, and key mRNA and protein expression levels. Results: The miRNA-mRNA gene interaction network suggests that hsa-miR-21-5p/phosphatase and tensin homolog (PTEN) are positioned at the core of this interaction network. Enzyme-linked immunosorbent assay (ELISA) and histological examination (HE) results suggest that pancreatic damage increased in the miR-21 inhibitor and EXW groups, whereas it decreased in the miR-21 activator and EXC groups compared to the EX group. PCR, western blot (WB), and TdT-mediated dUTP Nick-End Labeling (TUNEL) results indicate that hucMSC-Ex carrying hsa-miR-21-5p suppresses excessive activation of PTEN by phosphoinositide 3-kinase (PI3K), exerting therapeutic effects. Conclusion: This study has discovered that hucMSC-Ex effectively inhibits the translation of PTEN via the transported hsa-miR-21-5p, consequently affecting the PI3K/serine-threonine kinase (AKT) signaling pathway. This results in reduced inflammation and inhibition of acinar cell apoptosis by regulating pancreatic enzyme leakage, thereby providing a therapeutic effect on TP.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"8883585"},"PeriodicalIF":3.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stromal Vascular Fraction Therapy to Reduce Inflammation and Improve Cartilage Regeneration in Osteoarthritis Nude Rats. 基质血管部分疗法减轻骨关节炎裸鼠炎症和促进软骨再生。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-02-27 eCollection Date: 2025-01-01 DOI: 10.1155/sci/5356264

Xuan-Qi Zheng, Tong Wu, Minwei Zhao, Chun-Li Song

{"title":"Stromal Vascular Fraction Therapy to Reduce Inflammation and Improve Cartilage Regeneration in Osteoarthritis Nude Rats.","authors":"Xuan-Qi Zheng, Tong Wu, Minwei Zhao, Chun-Li Song","doi":"10.1155/sci/5356264","DOIUrl":"https://doi.org/10.1155/sci/5356264","url":null,"abstract":"Aims: To evaluate the efficacy of stromal vascular fraction (SVF) in treating osteoarthritis (OA). Background: OA is a common degenerative disease, the most important manifestation of which is cartilage destruction and inflammation. The SVF is a mixed group of multiple cells extracted from adipose tissue with a certain ability to promote tissue repair. However, the biological safety and efficacy of human derived SVF in treating OA have not been confirmed. Methods: Seventy-six nude rats were used in this experiment. The rat OA model was constructed with anterior cruciate ligament transection (ACLT). After 4 weeks, SVF cells were injected into the joint cavity once. After 12 weeks, the experimental animals were sacrificed and decalcified sections were subjected to hematoxylin and eosin (H&E), safranine O staining, and AP-PAS staining and immunohistochemistry for inflammation markers. Results: After surgery, the knee joint swells, pain intensifies, and the joint space narrows. The results of H&E, safranine O, and AP-PAS staining showed that the cartilage tissue was damaged in the ACLT-OA group and the treatment of SVF can reduce cartilage degradation. The numbers of ADAMTS-5-, MMP-13-, and IL-1β-positive cells significantly decreased and type II collagen-positive cells were more frequently detected in the ACLT-OA group compared with that in the control group, the treatment of SVF can reduce inflammation. Conclusion: SVF cells can be safely used to treat OA and can both effectively reduce the progression of joint inflammation and promote cartilage regeneration.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"5356264"},"PeriodicalIF":3.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway. 脂肪来源的干细胞外泌体通过miR-204-5p/TGF-β1/Smad途径促进糖尿病伤口无疤痕愈合

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-02-19 eCollection Date: 2025-01-01 DOI: 10.1155/sci/6344844

Peijun Song, Qiu Liang, Xiuyu Ge, Danlian Zhou, Mei Yuan, Weiwei Chu, Jing Xu

{"title":"Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway.","authors":"Peijun Song, Qiu Liang, Xiuyu Ge, Danlian Zhou, Mei Yuan, Weiwei Chu, Jing Xu","doi":"10.1155/sci/6344844","DOIUrl":"10.1155/sci/6344844","url":null,"abstract":"Numerous researches have demonstrated the therapeutic potential of adipose-derived stem cell exosomes (ADSC-Exos) in promoting wound healing. In this study, we aimed to investigate the impact of ADSC-Exos on diabetic wound fibroblasts and elucidate its possible mechanisms. CCK-8, Edu, cell scratch, and Transwell tests were used to evaluate the function of ADSC-Exos on rat skin fibroblasts (RSFs) in high-glucose (HG) medium. The targeting effect of ADSC-Exo-derived microRNA (miRNA) and TGF-β1 was assessed using bioinformatic analysis and then confirmed with western blot and dual luciferase reporter assays. ADSC-Exos, miR-204-5p mimic, and anti-miR-204-5p mimic were used to stimulate RSFs, and the levels of TGF-β1/Smad pathway were analyzed by western blot. In vivo, digital photo and tissue section staining were used to evaluate the therapeutic effect of ADSC-Exos on diabetic wounds. The data showed that ADSC-Exos enhance the proliferation and migration of fibroblasts under HG conditions, reduce excessive myofibroblast differentiation and collagen deposition, and promote scarless healing of diabetic wounds. Additionally, miR-204-5p in ADSC-Exos targets TGF-β1 to inhibit p-Smad2/3, Col I, and alpha-smooth muscle actin (α-SMA), thereby reducing fibrosis. These findings suggest that ADSC-Exos have potential prospects for promoting diabetic wound healing.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"6344844"},"PeriodicalIF":3.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Different Cell Types on the Osteogenic Differentiation Process of Mesenchymal Stem Cells. 不同细胞类型对间充质干细胞成骨分化过程的影响。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI: 10.1155/sci/5551222

Zixin Wang, Lina Ren, Zhengtao Li, Qingyuan Qiu, Haonan Wang, Xin Huang, Dongyang Ma

引用次数: 0

Human iPSC Reprogramming Success: The Impact of Approaches and Source Materials. 人类iPSC重编程成功：方法和源材料的影响。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1155/sci/2223645

Tatyana Pozner, Christine Grandizio, Matthew W Mitchell, Nahid Turan, Laura Scheinfeldt

{"title":"Human iPSC Reprogramming Success: The Impact of Approaches and Source Materials.","authors":"Tatyana Pozner, Christine Grandizio, Matthew W Mitchell, Nahid Turan, Laura Scheinfeldt","doi":"10.1155/sci/2223645","DOIUrl":"10.1155/sci/2223645","url":null,"abstract":"Since their discovery, human induced pluripotent stem cells (hiPSCs) have been instrumental in biomedical research, particularly in the fields of disease modelling, drug screening and regenerative therapies. Their use has significantly increased over recent years driven by the ability of hiPSCs to provide differentiated cell models without requiring embryonic stem cells. Furthermore, the transition from integrating to non-integrating reprogramming methodologies has contributed to the increase in utilisation. This shift minimises the risk of genomic alterations, enhancing the safety and reliability of hiPSCs. However, the factors that contribute to reprogramming success are still not well understood. In this study, we conducted a comparative analysis of the most prevalent non-integrating reprogramming methods across a range of starting source materials to assess their impact on reprogramming success rates. We found that while source material does not significantly impact success rates, the Sendai virus reprogramming method yields significantly higher success rates relative to the episomal reprogramming method. Our findings offer important insights from a biobanking perspective, for which long-term reliability, integrity and reproducibility of hiPSCs are crucial.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"2223645"},"PeriodicalIF":3.8,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome Loaded in Microneedle Patch Ameliorates Renal Ischemia-Reperfusion Injury in a Mouse Model. 微针贴片负载外泌体改善小鼠肾缺血再灌注损伤模型。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-01-15 eCollection Date: 2025-01-01 DOI: 10.1155/sci/3106634

Samin Taghavi, Somayeh Keshtkar, Mozhgan Abedanzadeh, Mehrdad Hashemi, Reza Heidari, Samira Sadat Abolmaali, Mahintaj Dara, Mahdokht Hossein Aghdaei, Alireza Sabegh, Negar Azarpira

{"title":"Exosome Loaded in Microneedle Patch Ameliorates Renal Ischemia-Reperfusion Injury in a Mouse Model.","authors":"Samin Taghavi, Somayeh Keshtkar, Mozhgan Abedanzadeh, Mehrdad Hashemi, Reza Heidari, Samira Sadat Abolmaali, Mahintaj Dara, Mahdokht Hossein Aghdaei, Alireza Sabegh, Negar Azarpira","doi":"10.1155/sci/3106634","DOIUrl":"10.1155/sci/3106634","url":null,"abstract":"Introduction: Renal dysfunction due to ischemia-reperfusion injury (IRI) is a common problem after kidney transplantation. In recent years, studies on animal models have shown that exosomes derived from mesenchymal stem cells (MSC-Exo) play an important role in treating acute kidney injury (AKI) and promoting tissue repair. The microneedle patch provides a noninvasive and targeted delivery system for exosomes. The purpose of this innovative approach is to combine MSC-Exo with microneedle patches. Method: Exosomes were isolated from MSCs, characterized, and placed in the prepared microneedle patch. Then this construct was applied to the IRI mice model. After 7 days, the gene expression of miR-34a and its targets B-cell lymphoma-2 (BCL-2) and BCL-2-associated X (BAX), along with reactive oxygen species (ROS) and lipid peroxidation (LPO) production, was investigated. Additionally, renoprotection was evaluated for measuring blood urea nitrogen (BUN) and creatinine (Cr) and histopathology detection. Results: After using microneedle patches containing exosomes, the reduction of miR-34a and BAX and enhancement of BCL-2 were observed. Moreover, treatment by this construct decreased the production of ROS, LPO, BUN, and Cr and improved tissue damage. Conclusion: The use of a microneedle patch containing exosomes is a noninvasive method that enables the release of exosomes in a slow manner. In comparison to exosome injection alone, microneedle patch-exosome treatment offers a longer and more targeted effect that improves renal IRI dysfunction and reduces tissue damage, potentially facilitating the clinical application of exosomes and improving graft survival.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"3106634"},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice. 人脐带间充质干细胞联合低剂量尼达尼布比单独使用更能减轻小鼠肺纤维化。

IF 3.8 3区医学

Stem Cells International Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.1155/sci/9445735

Huijun Qiu, Rong Zhang, Daozhu Si, Yi Shu, Jiang Liu, Yunqiu Xia, Ou Zhou, Wen Tan, Ke Yang, Daiyin Tian, Zhengxiu Luo, Enmei Liu, Lin Zou, Zhou Fu, Danyi Peng

{"title":"Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice.","authors":"Huijun Qiu, Rong Zhang, Daozhu Si, Yi Shu, Jiang Liu, Yunqiu Xia, Ou Zhou, Wen Tan, Ke Yang, Daiyin Tian, Zhengxiu Luo, Enmei Liu, Lin Zou, Zhou Fu, Danyi Peng","doi":"10.1155/sci/9445735","DOIUrl":"10.1155/sci/9445735","url":null,"abstract":"Pulmonary fibrosis (PF) is a lethal pathological change of fibrotic interstitial lung diseases (ILDs) with abundant fibroblasts proliferation after severely or continually alveolar epithelial cells (AECs) injury. Barely therapies are helpful for PF. Here we use bleomycin intratracheally injection to model PF with or without human umbilical cord-mesenchymal stem cells (hUC-MSCs) and/or nintedanib intervention. RNA-Seq followed with real-time PCR and western blot were used to find out the specific possible mechanisms of the effects of hUC-MSC and nintedanib on PF. Immunostaining, cell counting kit-8 (CCK-8), and 5-bromo-2'-deoxyuridine (BrdU) incorporation assay were used to detect the cell proliferation in vivo or in vitro separately. We found that hUC-MSCs alone had prophylactic, but not therapeutic effects on bleomycin induced mouse PF. Nevertheless, the combination therapy of hUC-MSCs and low-dose nintedanib significantly improved survival and reversed lung fibrosis in PF model mice. The factors secreted by hUC-MSCs have promotional effects on the proliferation both of fibroblasts and AECs. Nintedanib could hamper the facilitation of fibroblasts caused by hUC-MSCs without influence on AECs proliferation, which might be related with the inhibition on FGFR, PDGFR, and VEGFR activities. Our study indicated that the combination therapy of hUC-MSCs and nintedanib should be a promising strategy for PF.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2025 ","pages":"9445735"},"PeriodicalIF":3.8,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noggin Combined With Human Dental Pulp Stem Cells to Promote Skeletal Muscle Regeneration. Noggin联合人牙髓干细胞促进骨骼肌再生。

IF 3.8 3区医学

Stem Cells International Pub Date : 2024-12-28 eCollection Date: 2024-01-01 DOI: 10.1155/sci/2812390

Meng-Han Zhang, Li-Ming Yu, Wei-Hua Zhang, Jia-Jia Deng, Bing-Jing Sun, Mei-Hua Chen, Wei Huang, Jiao Li, Hua He, Xin-Xin Han, Yue-Hua Liu

{"title":"Noggin Combined With Human Dental Pulp Stem Cells to Promote Skeletal Muscle Regeneration.","authors":"Meng-Han Zhang, Li-Ming Yu, Wei-Hua Zhang, Jia-Jia Deng, Bing-Jing Sun, Mei-Hua Chen, Wei Huang, Jiao Li, Hua He, Xin-Xin Han, Yue-Hua Liu","doi":"10.1155/sci/2812390","DOIUrl":"https://doi.org/10.1155/sci/2812390","url":null,"abstract":"A proper source of stem cells is key to muscle injury repair. Dental pulp stem cells (DPSCs) are an ideal source for the treatment of muscle injuries due to their high proliferative and differentiation capacities. However, the current myogenic induction efficiency of human DPSCs hinders their use in muscle regeneration due to the unknown induction mechanism. In this study, we treated human DPSCs with Noggin, a secreted antagonist of bone morphogenetic protein (BMP), and discovered that Noggin can effectively promote myotube formation. We also found that Noggin can accelerate the skeletal myogenic differentiation (MyoD) of DPSCs and promote the generation of Pax7+ satellite-like cells. Noggin increased the expression of myogenic markers and the transcriptional and translational abundance of satellite cell (SC) markers in DPSCs. Moreover, BMP4 inhibited Pax7 expression and activated p-Smad1/5/9, while Noggin eliminated BMP4-induced p-Smad1/5/9 in DPSCs. This finding suggests that Noggin antagonizes BMP by downregulating p-Smad and facilitates the MyoD of DPSCs. Then, we implanted Noggin-pretreated DPSCs combined with Matrigel into the mouse tibialis anterior muscle with volumetric muscle loss (VML) and observed a 73% reduction in the size of the defect and a 69% decrease in scar tissue. Noggin-treated DPSCs can benefit the Pax7+ SC pool and promote muscle regeneration. This work reveals that Noggin can enhance the production of satellite-like cells from the MyoD of DPSCs by regulating BMP/Smad signaling, and these satellite-like cell bioconstructs might possess a relatively fast capacity for muscle regeneration.","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2024 ","pages":"2812390"},"PeriodicalIF":3.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0