Stain technology最新文献_第4页

Improvement of the quality of frozen sections from formalin fixed tissue. 福尔马林固定组织冷冻切片质量的提高。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105607

T Ishii, K Kasama, M Kondo

引用次数: 3

Standard specimens for stain calibration: application to Romanowsky-Giemsa staining. 染色校正的标准标本:用于罗曼诺夫斯基-吉姆萨染色。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108058

J N Turner, B Weir, D N Collins

{"title":"Standard specimens for stain calibration: application to Romanowsky-Giemsa staining.","authors":"J N Turner, B Weir, D N Collins","doi":"10.3109/10520299009108058","DOIUrl":"https://doi.org/10.3109/10520299009108058","url":null,"abstract":"Standardized specimens with reproducible staining properties were fabricated from extracts of biological objects (bovine liver, nucleoprotamine and defatted muscle). The standard specimens were stained with two formulations of the Romanowsky-Giemsa stain (RG), using the same azure B and eosin Y. One formulation used methanol and Sorensen's buffer and the other DMSO and Hepes buffer as solvents. The standard specimens were stained either in the composite stain or in the individual dyes dissolved in the same solvents and at the same concentration as the composite stain. Solution spectroscopy demonstrated different spectra for the two formulations with some wavelength regions varying by more than an order of magnitude. The RG spectra were also very different from those of the individual dyes dissolved at the RG concentration in the respective solvents. The stained standard specimens were analyzed by microspectrophotometry and were found to have spectra similar to those of cell smears. Furthermore, the standard specimens were shown to be a repeatable substrate for stain uptake. The transmitted light intensity from random fields of the same standardized specimen varied +/- 5%. When specimens were stained at the same time, the specimen-to-specimen variation depended on preparation conditions and the measurement wavelength, but was as good as +/- 5% for some conditions. The quantitative stain performance of both formulations was studied and compared. The standardized specimens provide a tool for the quantitative study of staining processes and specimen preparation procedures and for stain calibration.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 2","pages":"55-67"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12858870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Use of DNA fluorochromes for studying meiosis in the woody species Thryptomene calycina. 应用DNA荧光染料研究木本植物红萼花的减数分裂。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108069

D Beardsell, R B Knox, E Williams

引用次数: 2

Electron dense artefactual deposits in tissue sections: the role of ethanol, uranyl acetate and phosphate buffer. 组织切片中电子致密的人工沉积物:乙醇、醋酸铀酰和磷酸盐缓冲液的作用。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105614

J Louw, K Williams, I S Harper, S A Walfe-Coote

{"title":"Electron dense artefactual deposits in tissue sections: the role of ethanol, uranyl acetate and phosphate buffer.","authors":"J Louw, K Williams, I S Harper, S A Walfe-Coote","doi":"10.3109/10520299009105614","DOIUrl":"https://doi.org/10.3109/10520299009105614","url":null,"abstract":"The occurrence of electron dense deposits in sections of aldehyde-fixed tissue prepared for transmission electron microscopy has been attributed to a number of conflicting factors. In an attempt to clarify this, the precipitating effect of different combinations of phosphate or cacodylate buffer, glutaraldehyde, ethanol and uranyl acetate was investigated in test tubes. As a preliminary investigation the combination of phosphate buffer, ethanol and uranyl acetate was investigated in heart and kidney tissue fixed in glutaraldehyde with or without postosmication. The essential factors in the formation of electron dense deposits in these tissues appear to be phosphate buffer, ethanol, and uranyl acetate, although glutaraldehyde may contribute in some way. The nature and intensity of the deposits seem to vary with the sequence of combination of these factors. Osmium did not appear to be an essential factor in the reaction since deposits were observed in both osmicated and unosmicated tissue. To avoid such deposits, a postosmication distilled water wash for 20 to 30 min followed by en bloc staining with aqueous uranyl acetate is advised if phosphate buffer is used as a fixative vehicle or buffer wash after the primary fixative.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 5","pages":"243-50"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12868506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effects of fluorescent brighteners on growth and morphology of the red alga Antithamnion kylinii. 荧光增白剂对红藻生长和形态的影响。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105622

D J Belliveau, D J Garbary, J L McLachlan

引用次数: 8

A technique for the combination of clearing, staining, and injecting small mammals. 一种结合清除、染色和注射小型哺乳动物的技术。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139923

K M Dudzinski, N A Neff

引用次数: 5

Inhibition reactivation myofibrillar ATPase technique for demonstration of three fiber types in a single cryostat muscle section. 抑制再激活肌纤维atp酶技术在一个单一的低温冷冻肌肉切片中显示三种纤维类型。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108061

V Horák, S Matolín

引用次数: 4

Determination of plasma cell labelling index with bromodeoxyuridine using a double immunoenzymatic technique. 双免疫酶法测定溴脱氧尿苷浆细胞标记指数。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105605

M Ffrench, J P Magaud, P Duhaut, P A Bryon, J J Viala

引用次数: 7

Effect of sodium hexanitrocobaltate (III) decomposition on its staining of intracellular potassium ions. 六硝基钴酸钠(III)分解对细胞内钾离子染色的影响。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105603

D B Green, S M Dodge, J R Lee, G Tallman

{"title":"Effect of sodium hexanitrocobaltate (III) decomposition on its staining of intracellular potassium ions.","authors":"D B Green, S M Dodge, J R Lee, G Tallman","doi":"10.3109/10520299009105603","DOIUrl":"https://doi.org/10.3109/10520299009105603","url":null,"abstract":"The effect was examined of the chemical decomposition of the potassium stain sodium hexanitrocobaltate (III) (SHC), on its ability to produce stain granules of consistent size that could be used to estimate the K+ contents of stomatal guard cells. Stomata in detached epidermis from leaves of Vicia faba (fava bean) were stimulated to accumulate K+ by treating them with fusicoccin. Stomatal apertures and the fraction of guard cell area covered by K+ precipitate granules (K+ score) were measured by digitizing photographic enlargements, and K+ scores were correlated with the age of stain that had been stored either in open or closed containers. The ability of stain aged in open containers to produce consistent fractional cell coverage was compared to 1) the ability of identically treated stain to precipitate K+ from solutions of KCI, and to 2) the kinetics of decomposition of SHC. It was found that the fractional coverage of guard cells of stomata opened to the same apertures decreased with a first order rate constant of 2.3 x 10(-5)/sec. The mass of precipitate formed by treatment of KCl solutions was unchanged for 2 hr after initial preparation of the SHC, and decreased thereafter with a first order rate constant of 1.0 x 10(-5)/sec. When stored in tightly sealed containers, nearly 100 hr were required for an occasionally opened bottle of SHC to decay to the same efficacy as a solution left open to the air for 8 hr.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 1","pages":"15-24"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105603","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12858867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Cytophotometric differentiation of high elevation spruces: physiological and ecological implications. 高海拔云杉的细胞光度分化:生理和生态意义。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105602

G P Berlyn, J L Royte, A O Anoruo

{"title":"Cytophotometric differentiation of high elevation spruces: physiological and ecological implications.","authors":"G P Berlyn, J L Royte, A O Anoruo","doi":"10.3109/10520299009105602","DOIUrl":"https://doi.org/10.3109/10520299009105602","url":null,"abstract":"Red and black spruce and their hybrids can be determined by morphological indices; however, the criteria are somewhat subjective and increasingly difficult to use at higher elevations. Although the chromosome number is identical (2n = 24), red spruce has twice as much nuclear DNA (48 pg) than black spruce (24 pg) and thus the species and their hybrids can also be separated by cytophotometry. This is relevant to spruce decline studies because black spruce is much more resistant to high elevation environmental stresses, both natural and anthropogenic. It also has implications for the effect of climatic changes on the composition of high elevation spruce-fir forests because red spruce can outcompete black spruce under more mesic conditions. Four elevation transects sampling spruce on the east and west sides of Mount Washington (New Hampshire) and Camels Hump (Vermont) and a single transect on the southwest side of Whiteface Mountain (New York) were made to investigate the degree of hybridization and introgression between these two species. A positive correlation was found between increased elevation and increased black spruce genes on Mount Washington and Camels Hump. Pure black spruce was found on Mount Washington from 1356 m to 1582 m. No pure black or red spruce was found on Camels Hump although the proportion of red spruce alleles was significantly greater on Camels Hump. All trees sampled at all elevations on Whiteface Mountain were pure red spruce. Thus the proportion of black spruce alleles in high elevation spruce populations decreases from east to west. This closely parallels the increase in spruce decline which increases from east to west.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13508989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34