一种结合清除、染色和注射小型哺乳动物的技术。

K M Dudzinski, N A Neff
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引用次数: 5

摘要

以小家鼠为实验对象,建立了一种小型哺乳动物软组织清除、软骨和骨骼染色、血管系统注射的联合方法。由于肌内脂肪含量相对较高,哺乳动物肌肉组织在用以前的技术“清理”后仍呈乳白色,甚至不透明。一种使用氯仿-乙醇的方法成功地使哺乳动物标本的软组织半透明,而不会因注射到循环系统中的乳胶而损坏或出血。由此产生的标本可以很好地显示骨骼系统和注射血管系统,没有不透明组织的阻塞或物理移除结缔组织的破坏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A technique for the combination of clearing, staining, and injecting small mammals.

A combined method for clearing soft tissues, staining cartilage and bone, and injecting the vascular system of small mammals was developed using Mus musculus (house mouse). Mammalian muscle tissue remains milky or even opaque after "clearing" by previous techniques due to the relatively high content of intramuscular fat. A method employing chloroform-ethanol successfully renders soft tissues of mammalian specimens translucent without damaging or bleeding color from the latex injected in the circulatory system. Resulting specimens yield an excellent view of the skeletal system and the injected vascular system without obstruction by opaque tissues or disruption by physical removal of connective tissue.

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