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Cleaning resin sections with a glass knife for light microscopy. 用光学显微镜用玻璃刀清洗树脂切片。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108064
F Yasuzumi, A Ishida, N Okura
{"title":"Cleaning resin sections with a glass knife for light microscopy.","authors":"F Yasuzumi, A Ishida, N Okura","doi":"10.3109/10520299009108064","DOIUrl":"https://doi.org/10.3109/10520299009108064","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 2","pages":"103-4"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13508992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction. 一种利用树脂萃取的塑料包埋材料提高荧光显微镜分辨率的方法。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105616
S D Russell
{"title":"A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.","authors":"S D Russell","doi":"10.3109/10520299009105616","DOIUrl":"https://doi.org/10.3109/10520299009105616","url":null,"abstract":"<p><p>A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 5","pages":"259-61"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105616","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13429248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Modified methodology to improve flow cytometric DNA histograms from paraffin-embedded material. 改进了石蜡包埋材料流式细胞DNA直方图的方法。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105619
D D McLemore, A el Naggar, L C Stephens, J H Jardine
{"title":"Modified methodology to improve flow cytometric DNA histograms from paraffin-embedded material.","authors":"D D McLemore,&nbsp;A el Naggar,&nbsp;L C Stephens,&nbsp;J H Jardine","doi":"10.3109/10520299009105619","DOIUrl":"https://doi.org/10.3109/10520299009105619","url":null,"abstract":"<p><p>Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1%. Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 6","pages":"279-91"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13236260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Sections from double mesas obtained at the same tissue plane. 在同一组织平面上获得的双台地切片。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139922
G Ellender
{"title":"Sections from double mesas obtained at the same tissue plane.","authors":"G Ellender","doi":"10.3109/10520299009139922","DOIUrl":"https://doi.org/10.3109/10520299009139922","url":null,"abstract":"<p><p>A method for obtaining sections from two areas in the face plane of a tissue block is described. It facilitates ultrathin sectioning where virtually identical planes of section are essential but where areas of interest are too far apart to be included in a single section. Two horizontally separated mesas are prepared; sections are cut from the first with the knife rotated around its vertical axis by 2-3 degrees to provide clearance for the other. The second mesa is then sectioned with the knife rotated 4-6 degrees in the opposite direction. Similarly, by changing the vertical inclination of the block, two additional vertically separated mesas can be cut. This procedure is of great value for comparative morphometric studies of material from opposite sides of individual specimens.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 3","pages":"107-11"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009139922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13526971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Demonstration of sensory-motor innervation of postmetamorphic forelimb regenerates of Triturus alpestris (Urodela) on cryotome serial sections using horseradish peroxidase. 利用辣根过氧化物酶在冷冻切片上展示变形后的前肢再生的感觉-运动神经支配。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139924
H J Anton, A Oberemm
{"title":"Demonstration of sensory-motor innervation of postmetamorphic forelimb regenerates of Triturus alpestris (Urodela) on cryotome serial sections using horseradish peroxidase.","authors":"H J Anton,&nbsp;A Oberemm","doi":"10.3109/10520299009139924","DOIUrl":"https://doi.org/10.3109/10520299009139924","url":null,"abstract":"<p><p>A method has been developed to obtain horseradish peroxidase-treated serial sections containing spinal cord as well as bilateral ventral and dorsal roots, dorsal root ganglia and spinal nerves. Young postmetamorphic newts (Triturus alpestris) served as experimental animals. After cryotome cross sectioning the forelimb region of the trunk, slices 80 microns in thickness were mounted serially with up to 15 sections per slide. This facilitated subsequent staining manipulations and made partial loss of sections less likely.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 3","pages":"119-23"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009139924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13526972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy. 光散射扫描电镜对淋巴管的酶组织化学鉴定。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139926
S Kato
{"title":"Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy.","authors":"S Kato","doi":"10.3109/10520299009139926","DOIUrl":"https://doi.org/10.3109/10520299009139926","url":null,"abstract":"<p><p>The walls of lymphatics are characterized by strong 5'-nucleotidase activity, whereas those of blood capillaries reveal significantly lower or no activity. Alkaline phosphatase activity, on the other hand, is markedly higher in blood capillaries than in lymphatic vessels. On the basis of such characteristics, lymphatics and blood capillaries were distinguished histochemically in rat stomach using 5'-nucleotidase-alkaline phosphatase double staining. The distribution and intensity of lead-demonstrated 5'-nucleotidase activity in lymphatic vessels could be determined by comparing the images of the same histochemically stained cryostat section as seen by light and backscattered image scanning electron microscopy. The specificity of the 5'-nucleotidase reaction was obtained by inhibiting nonspecific alkaline phosphatase by including L-tetramisole in the 5'-nucleotidase incubation medium. The products of the 5'-nucleotidase activity were deposited on the outer surface of the plasma membrane of the lymphatic endothelial cells.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 3","pages":"131-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009139926","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13526974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 37
Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L. 利用对苯二胺在花生组织中定位植物脂质。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108062
A K Bal
{"title":"Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L.","authors":"A K Bal","doi":"10.3109/10520299009108062","DOIUrl":"https://doi.org/10.3109/10520299009108062","url":null,"abstract":"<p><p>p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need no further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 2","pages":"91-4"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12858871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Stabilization of pre-epithelial mucus gel in cryostat sections from rat colon with celloidin. 用纤维素蛋白稳定大鼠结肠低温切片上皮前黏液凝胶。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108067
L Szentkuti, A Eggers
{"title":"Stabilization of pre-epithelial mucus gel in cryostat sections from rat colon with celloidin.","authors":"L Szentkuti,&nbsp;A Eggers","doi":"10.3109/10520299009108067","DOIUrl":"https://doi.org/10.3109/10520299009108067","url":null,"abstract":"Gastrointestinal mucus occurs partly as a stable, translucent water-insoluble gel adherent to the mucosal surface, and partly in a water soluble form in the lumen (Allen and Carroll 1985). The adherent pre-epithelial mucus gel (PMG) has been shown in unfixed sections of rat stomach as a continuous layer of median thickness 80 μm (Kerss et al. 1982), and 145 μm (Sandzen et al. 1988), respectively.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 4","pages":"179-81"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12864010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Chlorotetracycline induced fluorescence of antherozoids in ferns. 氯四环素诱导蕨类植物拟花类的荧光。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139925
J Singh, S Devi, M Gupta
{"title":"Chlorotetracycline induced fluorescence of antherozoids in ferns.","authors":"J Singh,&nbsp;S Devi,&nbsp;M Gupta","doi":"10.3109/10520299009139925","DOIUrl":"https://doi.org/10.3109/10520299009139925","url":null,"abstract":"<p><p>A quick method based on chlorotetracycline induced fluorescence of the antherozoids is described. This method was found suitable for studying the number and duration of motility of antherozoids in toxicity studies where male fertility is a significant character.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 3","pages":"125-30"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009139925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13526973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of 5'-adenylylimidodiphosphate-hydrolyzing enzyme activity in rabbit taste bud cells using X-ray microanalysis. 用x射线微量分析鉴定兔味蕾细胞中5′-腺苷酸二磷酸水解酶的活性。
Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108059
N Asanuma
{"title":"Identification of 5'-adenylylimidodiphosphate-hydrolyzing enzyme activity in rabbit taste bud cells using X-ray microanalysis.","authors":"N Asanuma","doi":"10.3109/10520299009108059","DOIUrl":"https://doi.org/10.3109/10520299009108059","url":null,"abstract":"<p><p>X-ray microanalysis has been used to characterize the enzyme activity hydrolyzing the ATP analogue 5'-adenylylimidodiphosphate (AMP-PNP) in taste bud cells. Rabbit foliate papillae fixed with paraformaldehyde and glutaraldehyde were incubated cytochemically with AMP-PNP as the substrate and lead ion as capture agent. The reaction product which appeared on the microvilli of taste bud cells was examined using an energy dispersive X-ray microanalyzer connected to an analytical electron microscope. The X-ray spectrum thus obtained was compared with that obtained from the product obtained from the demonstration of ATPase activity. Comparison of the phosphorus/lead ratios in the two products showed that twice as much phosphorus was released from an AMP-PNP molecule by the activity in question compared with that released from an ATP molecule by ATPase activity. This indicates that the enzyme hydrolyzes AMP-PNP into AMP and imidodiphosphate and that the enzyme is adenylate cyclase or ATP pyrophosphohydrolase, which possesses a similar hydrolytic property, but not ATPase or alkaline phosphatase, which hydrolyzes AMP-PNP into ADP-NH2 and orthophosphate. This paper provides an example of the use of X-ray microanalysis as a tool for enzyme distinction. The method is applicable to a variety of enzymes and tissues.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 2","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13316169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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