Stain technology最新文献_第2页

Vital fluorescent staining technique for microspores of Brassica napus. 甘蓝型油菜小孢子生命荧光染色技术。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105615

E B Swanson, S A Yarrow, M P Coumans, L R Erickson

引用次数: 3

Quantitative ultrastructural immunocytochemistry using a computerized image analysis system. 定量超微结构免疫细胞化学使用计算机图像分析系统。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105618

S Eneström, B Kniola

引用次数: 14

A new technique for C-banding. c带的新技术。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105609

J D Bugallo, R Teresa, M Vallejo

引用次数: 1

Visual enhancement of myelinated tissues in the central nervous system of the rat using Sudan black B. 苏丹黑B对大鼠中枢神经系统髓鞘组织的视觉增强作用。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139928

K R Olson, R K Traub

引用次数: 4

Modified Golgi method for whole rat brain. 改进的大鼠全脑高尔基法。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009139929

E R Greer, C K Jen

引用次数: 0

Cement line staining in undecalcified thin sections of cortical bone. 骨皮质未钙化薄切片的水泥线染色。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108065

S D Bain, T M Impeduglia, C T Rubin

引用次数: 31

A simple method for improved visualization of the lamellated structure of cutinized and suberized plant cell walls by electron microscopy. 一个简单的方法，提高可视化的层状结构的角质化和隐性植物细胞壁的电子显微镜。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108068

H G Heumann

引用次数: 6

A metachromatic dye-ATPase method for the simultaneous identification of skeletal muscle fiber types I, IIA, IIB and IIC. 一种同时鉴定骨骼肌纤维类型I、IIA、IIB和IIC的渐变色染料- atp酶法。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105613

R W Ogilvie, D L Feeback

{"title":"A metachromatic dye-ATPase method for the simultaneous identification of skeletal muscle fiber types I, IIA, IIB and IIC.","authors":"R W Ogilvie, D L Feeback","doi":"10.3109/10520299009105613","DOIUrl":"https://doi.org/10.3109/10520299009105613","url":null,"abstract":"The histochemical demonstration of quantitative differences in myofibrillar ATPase activity at the selective pH optima of the various types of human skeletal muscle fibers is the most widely used technique for their differentiation. The basis of the reaction is the deposition of insoluble salts of inorganic phosphate cleaved from ATP by myofibrillar ATPase(s) followed by substitution of the phosphates with less soluble chromogenic salts. Doriguzzi and associates reported using metachromatic dyes to demonstrate quantitative differences in phosphate deposition among different fiber types. Following routine ATPase histochemistry and staining with either azure A or toluidine blue, fibers with low ATPase activity (and low phosphate content) were stained metachromatically while fibers with high ATPase activity (and high phosphate content) were orthochromatic with the intensity of color proportional to the content of insoluble phosphate. The metachromasia was readily lost after immoderate washing in aqueous solutions or routine dehydration in ethanol, with consequent diminished fiber type distinction. A critical modification of this technique is reported in which incubation of frozen sections of human skeletal muscle in ATP-containing medium is carried out at room temperature (22-24 C), rather than the usual 37 C, followed by a revised washing and dehydration protocol. With these modifications, the four human skeletal muscle fiber types (types I, IIA, IIB, and IIC) can be identified rapidly and reliably in single sections, obviating the need for examination of serial sections. The tinctorial differentiation allows fiber type identification even in black and white photographs.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 5","pages":"231-41"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009105613","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12868505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 122

A simple method for the cultivation of cell monolayers for electron microscopy studies. 一种用于电镜研究的细胞单层培养的简单方法。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009105610

R M Pintó, J Jofre, A Bosch

引用次数: 2

Electron microscopic identification and morphologic preservation of enriched populations of lung cells isolated by laser flow cytometry and cell sorting: a new technique. 激光流式细胞术和细胞分选分离的肺细胞富集群体的电镜鉴定和形态学保存:一项新技术。

Stain technology Pub Date : 1990-01-01 DOI: 10.3109/10520299009108066

D P Penney, J F Leary, R A Cooper, A Paxhia

{"title":"Electron microscopic identification and morphologic preservation of enriched populations of lung cells isolated by laser flow cytometry and cell sorting: a new technique.","authors":"D P Penney, J F Leary, R A Cooper, A Paxhia","doi":"10.3109/10520299009108066","DOIUrl":"https://doi.org/10.3109/10520299009108066","url":null,"abstract":"There is increasing need to verify the identities of cell subpopulations enriched by laser flow cytometry and fluorescence-activated cell sorting (FACS). When cell subpopulations isolated from whole organs or tissues have similar characteristics (e.g., size, granularity, staining), light, phase contrast or fluorescence microscopy may not provide sufficient resolution to identify isolated cells accurately and many flow cytometric parameters (e.g., viability, fluorescence) require the cells to be live at the point of analysis where the cell transects the laser beam. In some studies, cells identified by fluorescence microscopy as a highly enriched subpopulation were found by electron microscopy to contain significant populations of other cell types. A technique, fixation-in-flow (FIF), has been developed to increase ability to correlate morphological and laser analyses of cell subpopulations. Sheath fluid is replaced by fixative, permitting fixation to be initiated immediately after laser beam analysis of live cells. This new procedure yields improved cytoarchitectural preservation of recovered cell subpopulation(s) for evaluation by transmission or scanning electron microscopy. This report presents results from applying the methodology to identify more accurately cell subpopulations of the distal lung, specifically type II pneumocytes, Clara cells and pulmonary macrophages. A modification of this procedure was employed to isolate fibroblast subpopulations from murine lung fibroblasts grown in vitro and the procedure is being used to determine the responses of cultured fibroblasts to other permutations (e.g., X-irradiation, cytokines).","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 4","pages":"165-77"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13370445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3