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A new method for demonstrating argyrophil cells of the pancreas and intestines. 胰、肠嗜银细胞的一种新方法。
Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108051
W Garvey, A Fathi, F Bigelow, B Carpenter, C Jimenez
{"title":"A new method for demonstrating argyrophil cells of the pancreas and intestines.","authors":"W Garvey,&nbsp;A Fathi,&nbsp;F Bigelow,&nbsp;B Carpenter,&nbsp;C Jimenez","doi":"10.3109/10520298909108051","DOIUrl":"https://doi.org/10.3109/10520298909108051","url":null,"abstract":"<p><p>A new method for demonstrating argyrophil cells of the pancreas and intestinal tract using a combined silver and reducing solution in sections of formaldehyde fixed tissue is described. Impregnating sections in a 60 C water bath, the procedure takes about 25 min. A microwave version that takes about 5 min is also given. Results are similar to those obtained with the Grimelius method for argyrophil cells.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13625151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
A new infusion needle for perfusion fixation. 一种新型灌注固定输液针制造技术。
Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108056
J Hiraoka, W Wang
{"title":"A new infusion needle for perfusion fixation.","authors":"J Hiraoka,&nbsp;W Wang","doi":"10.3109/10520298909108056","DOIUrl":"https://doi.org/10.3109/10520298909108056","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13625147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Staining of developing neurites with coomassie blue. 发育中的神经突用考马斯蓝染色。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108044
N G Carri, T Ebendal
{"title":"Staining of developing neurites with coomassie blue.","authors":"N G Carri,&nbsp;T Ebendal","doi":"10.3109/10520298909108044","DOIUrl":"https://doi.org/10.3109/10520298909108044","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13619660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections. 组织切片中皮肤硫酸酯蛋白多糖的免疫组织化学检测技术。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108042
M Sobue, J Takeuchi, T Fukatsu, T Nagasaka, N Nakashima, T Ogura, T Katoh, K Yoshida
{"title":"Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections.","authors":"M Sobue,&nbsp;J Takeuchi,&nbsp;T Fukatsu,&nbsp;T Nagasaka,&nbsp;N Nakashima,&nbsp;T Ogura,&nbsp;T Katoh,&nbsp;K Yoshida","doi":"10.3109/10520298909108042","DOIUrl":"https://doi.org/10.3109/10520298909108042","url":null,"abstract":"<p><p>Dermatan sulfate proteoglycan chains were detected in tissue sections treated with chondroitin B-lyase (0.01 units/ml) in 20 mM Tris-HCl (pH 8.0) for 1 hr, followed by staining with antibody 9A2 specific for unsaturated uronic acid coupled to N-acetylgalactosamine-4 sulfate. In contrast, after treatment with chondroitin B-lyase, no positive staining was observed with antibodies 3B3 and 1B5 which react to the unsaturated uronic acid coupled to N-acetylgalactosamine 6-sulfate and unsaturated uronic acid coupled to N-acetylgalactosamine, respectively. The distribution of dermatan sulfate thus revealed was confirmed by comparison with that found by monoclonal antibody 6B6 which reacts with small proteoglycans carrying dermatan sulfate side chains. The localization of positive staining in fibrous connective tissues was almost identical with these two procedures.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13880939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
A simple procedure to obtain one-micrometer sections of routinely embedded paraffin material. 一个简单的程序，以获得一微米切片常规包埋石蜡材料。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108041
L C Junqueira, M D Silva, H Torloni
{"title":"A simple procedure to obtain one-micrometer sections of routinely embedded paraffin material.","authors":"L C Junqueira,&nbsp;M D Silva,&nbsp;H Torloni","doi":"10.3109/10520298909108041","DOIUrl":"https://doi.org/10.3109/10520298909108041","url":null,"abstract":"<p><p>By freezing blocks of paraffin-embedded tissues to a convenient temperature it is possible to obtain routinely 1 micron sections that can be further processed as normal thicker sections. Normal and disposable steel knives can be used and the staining time should be increased in most procedures. Gradual freezing of blocks to the temperature of dry ice is the simplest and safest way to obtain an adequate temperature. The best results were obtained using as fixative 4% paraformaldehyde in phosphate buffered saline solution.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13804572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Histological preparation of implanted biomaterials for light microscopic evaluation of the implant-tissue interaction. 植入生物材料的组织学准备，用于植入物与组织相互作用的光镜评估。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108039
E Murice-Lambert, A B Banford, R L Folger
{"title":"Histological preparation of implanted biomaterials for light microscopic evaluation of the implant-tissue interaction.","authors":"E Murice-Lambert,&nbsp;A B Banford,&nbsp;R L Folger","doi":"10.3109/10520298909108039","DOIUrl":"https://doi.org/10.3109/10520298909108039","url":null,"abstract":"<p><p>A technique is presented for processing implanted biomaterials with surrounding soft tissue for histological assessment of the implant-tissue interaction. Specimens are removed with the implant-tissue interface intact, fixed in formalin, dehydrated in a graded series of ethanol followed by a graded series of acetone in ethanol, and embedded in Spurr's low viscosity epoxy resin. Sections 0.5-1.0 mm thick are cut from the cured blocks using a metallurigical saw with a diamond wafer blade. After being glued to glass microscope slides, they are ground and polished to approximately 75 microns in thickness. The polished sections are treated with 95% ethanol saturated with sodium hydroxide, stained with Gill's hematoxylin and counterstained in eosin Y-phloxine B. The sodium hydroxide solution degrades the resin, allowing the stain to penetrate the tissue. By limiting the time in sodium hydroxide, the depth of staining is controlled and one is able to simulate a thin paraffin section with high resolution of the implant-soft tissue interface.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13804571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
A new technique for staining catecholic residues in biological samples. 生物样品中儿茶酚残留染色新技术。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108038
R Kalyani, K Nellaiappan
{"title":"A new technique for staining catecholic residues in biological samples.","authors":"R Kalyani,&nbsp;K Nellaiappan","doi":"10.3109/10520298909108038","DOIUrl":"https://doi.org/10.3109/10520298909108038","url":null,"abstract":"<p><p>This technique for localizing catecholic residues in biological samples is based on the condensation of Besthorn's hydrazone (3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) with quinone residues obtained by the oxidation of catechols in the presence of ammonia. The product is a dark pink MBTH-quinone compound. This method is very sensitive and positive to catechol even at the 0.05 microgram level and the final product is chemically stable.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13619658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A rapid method for removing silver from stained chromosomes. 从染色染色体上快速去除银的方法。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108043
T E Denton
{"title":"A rapid method for removing silver from stained chromosomes.","authors":"T E Denton","doi":"10.3109/10520298909108043","DOIUrl":"https://doi.org/10.3109/10520298909108043","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13619659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells. 植物细胞核DNA序列测定及核仁银染色。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108037
J Dolezel, J Cíhalíková, O V Zakchlenjuk
{"title":"Sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells.","authors":"J Dolezel,&nbsp;J Cíhalíková,&nbsp;O V Zakchlenjuk","doi":"10.3109/10520298909108037","DOIUrl":"https://doi.org/10.3109/10520298909108037","url":null,"abstract":"<p><p>A method for sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells is described. Feulgen staining is done first and nuclear DNA estimated by absorption cytophotometry. Following this, the slides are stained with AgNO3. The method has been used to study the process of nucleolar fusion in garlic (Allium sativum L.) meristem root tip cells. It was found that during interphase nucleoli rarely fused, thus most fusion must have occurred before the G1 phase of the cell cycle.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13619661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Fixative evaluation and histologic appearance of embryonic rodent tissue. 啮齿动物胚胎组织的固定物评价和组织学外观。
Stain technology Pub Date : 1989-01-01 DOI: 10.3109/10520298909108036
W B Howard, C C Willhite, R A Smart
{"title":"Fixative evaluation and histologic appearance of embryonic rodent tissue.","authors":"W B Howard,&nbsp;C C Willhite,&nbsp;R A Smart","doi":"10.3109/10520298909108036","DOIUrl":"https://doi.org/10.3109/10520298909108036","url":null,"abstract":"<p><p>Histopathologic study of early hamster embryos was carried out after fixation in Zenker's solution, alcoholic formalin, Bouin's fluid, 10% neutral buffered formalin, or 3% glutaraldehyde and staining with hematoxylin and eosin. Fixation in Zenker's fluid followed by postfixation in neutral buffered formalin provided superior preservation of normal embryonic subcellular detail as compared to the other candidate processing techniques.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13804570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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