Stain technology最新文献_第9页

Staining of different tissues in thick epoxy resin-impregnated sections of human fetuses. 人类胎儿厚环氧树脂浸渍切片中不同组织的染色。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108049

H Fritsch

引用次数: 73

A new, rapid, microwave-stimulated method of staining melanocytic lesions. 一种新的、快速的、微波刺激的黑素细胞病变染色方法。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108050

A S Leong, P Gilham

引用次数: 21

Improving stain penetration into epoxy resin sections for light microscopy. 改善染色渗透到环氧树脂切片光学显微镜。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108054

H H Crowley

引用次数: 2

A high pH discontinuous buffer system for resolution of isozymes in starch gel electrophoresis. 淀粉凝胶电泳中同工酶的高pH不连续缓冲系统。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108047

P Chippindale

{"title":"A high pH discontinuous buffer system for resolution of isozymes in starch gel electrophoresis.","authors":"P Chippindale","doi":"10.3109/10520298909108047","DOIUrl":"https://doi.org/10.3109/10520298909108047","url":null,"abstract":"A Tris-citrate pH 9.5 gel/borate pH 8.2 electrode discontinuous buffer system for starch gel electrophoresis of proteins was developed to resolve iso- and allozymes of aspartate aminotransferase in frogs (Hyla crucifer). This buffer system also enhanced resolution of NADP-dependent malate dehydrogenase and the L-lactate dehydrogenase-A locus in this species. It provided good resolution of NAD-dependent malate dehydrogenase in esocid fishes, and esterases, glycerol-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, and S-aconitate hydratase in ambystomatid salamanders. Variation suppressed by other buffers was revealed by this buffer for some enzyme encoding loci, while at other loci, this buffer suppressed electromorph variability. The concentration of tris(hydroxymethyl)aminomethane in gels made with this buffer was much higher than in pH 8.7 \"Poulik\" gels, but running characteristics of the two gel types were similar. Gels made with this new buffer were less prone to splitting and \"warping\" than Poulik gels, and were easier to handle. When screening a given taxon for enzyme variability, tests using multiple buffers are essential to maximize the amount of electrophoretically detectable variation.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 2","pages":"61-4"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13939483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Paraffin sections of nervous tissue supravitally stained with methylene blue: a new, reliable and simple fixation technique. 亚甲基蓝染色神经组织上石蜡切片:一种新的、可靠的、简单的固定技术。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108052

T Müller

引用次数: 3

Method for the preservation of polystyrene latex beads in tissue sections. 组织切片中聚苯乙烯乳胶珠的保存方法。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108055

M Callebaut, C Meeussen

引用次数: 9

A simplified aluminum stain in paraffin sections of bone from hemodialysis patients. 血液透析患者骨石蜡切片的简化铝染色。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108046

Y Ohtsuki, T Yamaguchi, H Sonobe, K Takahashi, K Hayashi, A Takenaka, H Hashimoto, K Kuwabara, T Miyamoto, N Terao

{"title":"A simplified aluminum stain in paraffin sections of bone from hemodialysis patients.","authors":"Y Ohtsuki, T Yamaguchi, H Sonobe, K Takahashi, K Hayashi, A Takenaka, H Hashimoto, K Kuwabara, T Miyamoto, N Terao","doi":"10.3109/10520298909108046","DOIUrl":"https://doi.org/10.3109/10520298909108046","url":null,"abstract":"A new simplified method has been devised for staining aluminum and has been tested in paraffin sections of bone from 60 patients who have undergone hemodialysis. Iliac crest bone fragments were fixed in 20% phosphate-buffered formalin for less than a day and demineralized at room temperature in 10% phosphate-buffered formalin containing 5% formic acid for only 2 to 3 hr. Four-micron paraffin sections, accompanied by positive controls, were stained with Maloney's aluminum stain, the Berlin blue reaction for iron, dylon or Congo red for amyloid and von Kossa's reaction for calcium. Aluminum and iron were demonstrated particularly at the mineralizing front of bony tissues; aluminum in 52 cases, iron in 45. Dylon staining also gave positive results in 52 cases. It is important in determining whether aluminum deposition is present that the von Kossa reaction remains positive even after demineralization. This method may be more useful for demonstrating aluminum in bony tissues than the complicated and time-consuming resin-embedding method currently used.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 2","pages":"55-9"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13625148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Activated charcoal as a adsorbent of oxidized 3,3'-diaminobenzidine in peroxidase cytochemistry. 活性炭在过氧化物酶细胞化学中对氧化3,3′-二氨基联苯胺的吸附剂。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108053

A. Ros Barceló, R. Muñoz, F. Sabater

引用次数: 5

Transmission electron microscopic study of maize pachytene chromosome 6. 玉米粗线体6号染色体的透射电镜研究。

Stain technology Pub Date : 1989-03-01 DOI: 10.3109/10520298909108048

D L Shen, M Wu

{"title":"Transmission electron microscopic study of maize pachytene chromosome 6.","authors":"D L Shen, M Wu","doi":"10.3109/10520298909108048","DOIUrl":"https://doi.org/10.3109/10520298909108048","url":null,"abstract":"Cytoplasm-free chromosomes are frequently obtained in meiotic chromosome spreads prepared from mildly-fixed maize microsporocytes. These chromosomes are suitable for detailed structural analysis using a published electron microscopic technique. In the electron micrograph, the knobs and heterochromatin regions that have been used for karyotype analyses in the light microscope are clearly visible. Therefore, the electron microscopic map can be easily aligned with the traditional cytological map. In addition to these prominent structural features, numerous electron-dense bands also are observed. To determine whether the bands can be used as markers for the identification of each chromosomal subregion, the banding pattern of chromosome 6 is analyzed. Chromosome 6 is frequently associated with the nucleolus and can be easily recognized. We observed that at the zygotene stage in prophase I, electron-dense regions are detected on each homolog of the synapsing chromosome. During synapsis, the electron-dense regions on both homologs are brought into register to form more conspicuous bands. At the early pachytene stage, the banding pattern is stable and reproducible. Chromosome 6 contains eight dark bands, 19 medium bands and 14 light bands. The bands can be used as intrachromosomal markers for regional assignment of genes in detailed in situ hybridization mapping or cytogenetic studies. As the pachytene stage progresses, condensation of the chromosome bivalents is accompanied by fusion of adjacent bands.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 2","pages":"65-73"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909108048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13939484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Activated charcoal as a adsorbent of oxidized 3,3'-diaminobenzidine in peroxidase cytochemistry. 活性炭在过氧化物酶细胞化学中对氧化3,3′-二氨基联苯胺的吸附剂。

Stain technology Pub Date : 1989-03-01

A Ros Barceló, R Muñoz, F Sabater

引用次数: 0