发布求助
文献互助 智能选刊 最新文献

Stain technology最新文献

筛选
英文 中文
Polychrome staining of epoxy semithin sections using cacodylic buffer as a stain solvent. 用羧酸缓冲液作染色溶剂对环氧树脂半薄切片进行多色染色。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106989
S Pasyk, W Bartosik, A Fabry
{"title":"Polychrome staining of epoxy semithin sections using cacodylic buffer as a stain solvent.","authors":"S Pasyk, W Bartosik, A Fabry","doi":"10.3109/10520298909106989","DOIUrl":"https://doi.org/10.3109/10520298909106989","url":null,"abstract":"Many polychromatic stains are in use for epoxy-embedded tissues (Horobin 1983). We should like to report a quick and easy polychromatic staining procedure that we find useful for routine use. Formerly the stain we used was prepared in 20 ml water and 5 ml 96% alcohol, and gave polychromatic staining only at pH 7.4 obtained by the addition of 1 N NaOH. However, the stain gave satisfactory results only for two or three days. We found that stabilization of the staining solution through the use of an ethyl alcohol-cacodylic buffer solvent increased the life of the staining solution. This was convenient because the cacodylic buffer is used in our laboratory as a component of fixatives, and is not prepared specially for the staining.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"149"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A 595 nanometer band-pass filter enhances the contrast of in situ hybridization signals on chromosome observed after biotin/avidin-alkaline phosphatase localization. 595纳米带通滤波器增强了生物素/亲和素碱性磷酸酶定位后染色体原位杂交信号的对比度。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106986
H Yasue, T Awata, S Muramatsu
{"title":"A 595 nanometer band-pass filter enhances the contrast of in situ hybridization signals on chromosome observed after biotin/avidin-alkaline phosphatase localization.","authors":"H Yasue,&nbsp;T Awata,&nbsp;S Muramatsu","doi":"10.3109/10520298909106986","DOIUrl":"https://doi.org/10.3109/10520298909106986","url":null,"abstract":"<p><p>In situ hybridization with DNA probes labeled with biotin and detected by the avidin-alkaline phosphatase/5-bromo-chloroindoxyl phosphate-nitro blue tetrazolium system has been used to localize DNA sequences in chromosomes. To observe the hybridization signals, a phase contrast microscope has often been used because of the good visibility it provides. Use of a 595 nm band pass filter with the phase contrast microscope enhances signal contrast after in situ hybridization without reducing resolution.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"139-42"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
An improved flat embedding technique for immunoelectron microscopy. 一种改进的免疫电子显微镜平面包埋技术。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106987
S M Yu, I R Schwartz
{"title":"An improved flat embedding technique for immunoelectron microscopy.","authors":"S M Yu,&nbsp;I R Schwartz","doi":"10.3109/10520298909106987","DOIUrl":"https://doi.org/10.3109/10520298909106987","url":null,"abstract":"<p><p>Immunocytochemical staining has been widely used for localizing various hormonal antigens, protein markers and putative neurotransmitters in tissues. Immunostained sections can be examined light microscopically and specific areas selected for electron microscopic study.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"143-6"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Note from the Biological Stain Commission: certification of Wright stain solution. 来自生物染色委员会的说明:莱特染色液的认证。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106991
E A Schenk, C T Willis
{"title":"Note from the Biological Stain Commission: certification of Wright stain solution.","authors":"E A Schenk,&nbsp;C T Willis","doi":"10.3109/10520298909106991","DOIUrl":"https://doi.org/10.3109/10520298909106991","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"152-3"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106991","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
An improved method of making tissue carriers for TEM and SEM fixation. TEM和SEM固定用组织载体的改进制备方法。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106990
J F Pilger
{"title":"An improved method of making tissue carriers for TEM and SEM fixation.","authors":"J F Pilger","doi":"10.3109/10520298909106990","DOIUrl":"https://doi.org/10.3109/10520298909106990","url":null,"abstract":"Hazards in fixing small pieces of tissue for electron microscopy include damage, drying, or loss. Over the years, microstrainer tissue carriers have been developed to minimize these problems. Construction materials have included glass tubing, copper grids for electron microscopy, stainless steel screen, and bolting silk (Padawer 1951, Friend 1963, Bronskill 1970). Carriers made from plastic embedding molds (e.g., BEEM capsules) with either TEM grids attached to the conical tip (Buchanan 1965) or Nitex screen cloth held to one end by a retaining ring have proven to be inexpensive and popular, though the former has a very small filtration area and in the latter small tissues may be lost or crushed between the screen cloth and the bottom rim of the carrier. This note describes a carrier in which Nitex is permanently sealed to the bottom edee of a BEEM capsule cylinder.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"150-1"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106990","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13827541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A method for preparing and staining histological sections containing titanium implants for light microscopy. 一种制备和染色光学显微镜用含钛植入物的组织学切片的方法。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106984
K Gotfredsen, E Budtz-Jörgensen, L N Jensen
{"title":"A method for preparing and staining histological sections containing titanium implants for light microscopy.","authors":"K Gotfredsen,&nbsp;E Budtz-Jörgensen,&nbsp;L N Jensen","doi":"10.3109/10520298909106984","DOIUrl":"https://doi.org/10.3109/10520298909106984","url":null,"abstract":"<p><p>An improved method for preparing and staining ground tissue-implant sections for light microscopy is presented. Undecalcified tissue blocks with titanium implants were dehydrated in an ascending series of ethanol and stained in toto with basic fuchsin. Specimens were infiltrated and embedded in methyl methacrylate and sections were prepared using a cutting-grinding-system. The polished surface was counterstained with light green or anilin blue. Light polymerizing resin was used as slide mounting medium and for mounting the coverglass. The sections obtained were 10-15 microns thick with tissue architecture which clearly differentiated structures at the tissue-implant interface. The method was very useful for computer assisted morphometric analysis.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"121-7"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106984","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 79
Lymphocyte membrane antigens in glycol methacrylate embedded tissue. 甲基丙烯酸乙二醇包埋组织中的淋巴细胞膜抗原。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106982
V Glezerov, B Dorsett
{"title":"Lymphocyte membrane antigens in glycol methacrylate embedded tissue.","authors":"V Glezerov,&nbsp;B Dorsett","doi":"10.3109/10520298909106982","DOIUrl":"https://doi.org/10.3109/10520298909106982","url":null,"abstract":"<p><p>The use of formalin or Michel's solution either alone or in combination with acetone, and acetone, methanol or ethanol alone as fixatives, and glycol methacrylate as embedding medium were evaluated for their suitability in procedures to detect lymphocyte membrane antigens by OKT and Leu monoclonal antibodies in human tonsils. No staining was detected in sections fixed in 70% or absolute ethanol and embedded in glycol methacrylate with either the direct immunofluorescence or avidin-biotin methods. Fixation in Michel's solutions plus acetone at room temperature revealed staining by both. Neither method resulted in staining after fixation in Michel's solution plus acetone at 4 C presumably due to the slow action of the fixative. Staining was enhanced using a combination of primary and secondary biotinylated antibodies. Dual staining allowed concurrent detection of two antigens in the same section. Glycol methacrylate embedding is a possible replacement for ultracold storage in the preservation of tissue for immunofluorescent staining.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"105-12"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A versatile new mineralized bone stain for simultaneous assessment of tetracycline and osteoid seams. 一种多功能的新矿化骨染色剂，用于同时评估四环素和类骨接缝。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106985
A R Villanueva, K D Lundin
{"title":"A versatile new mineralized bone stain for simultaneous assessment of tetracycline and osteoid seams.","authors":"A R Villanueva,&nbsp;K D Lundin","doi":"10.3109/10520298909106985","DOIUrl":"https://doi.org/10.3109/10520298909106985","url":null,"abstract":"<p><p>A versatile mineralized bone stain (MIBS) for demonstrating osteoid seams and tetracycline fluorescence simultaneously in thin or thick undecalcified sections has been developed. Bone specimens are fixed in 70% ethanol, but 10% buffered formalin is permissible. Depending upon one's preference, these specimens can be left unstained or be prestained before plastic embedding. Osteoid seams are stained green to jade green, or light to dark purple. Mineralized bone matrix is unstained or green. Osteoblast and osteoclast nuclei are light to dark purple, cytoplasm varies from slightly gray to pink. The identification of osteoid seams by this method agrees closely with identification by in vivo tetracycline uptake using the same section from the same biopsy. The method demonstrates halo volumes, an abnormal, lacunar, low density bone around viable osteocytes in purple. This phenomenon is commonly seen in vitamin D-resistant rickets, fluorosis, renal osteodystrophy, hyperparathyroidism, and is sometimes seen in fluoride treated osteoporotic patients. In osteomalacic bone, most osteoid seams are irregularly stained as indicated by the presence of unmineralized osteoid between mineralized lamellae. The method has been used effectively in staining new bone formation in hydroxyapatite implants and bone grafts. Old, unstained, plastic embedded undecalcified sections are stained as well as fresh sections after removal of the coverslip. This stain also promises to be valuable in the study of different metabolic bone diseases from the point of view of remodeling, histomorphometry, and pathology.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"129-38"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 98
Thiéry test in combination with a uranyl acetate-lead citrate staining. 硫胺嘧啶试验结合醋酸铀酰-柠檬酸铅染色。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106988
M Weber
{"title":"Thiéry test in combination with a uranyl acetate-lead citrate staining.","authors":"M Weber","doi":"10.3109/10520298909106988","DOIUrl":"https://doi.org/10.3109/10520298909106988","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"147-9"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106988","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Effect of cytochrome c concentration on the ultrastructural appearance of bovine nasal cartilage proteoglycans. 细胞色素c浓度对牛鼻软骨蛋白聚糖超微结构外观的影响。
Stain technology Pub Date : 1989-05-01 DOI: 10.3109/10520298909106983
P Front, C Dauguet, D R Mitrovic
{"title":"Effect of cytochrome c concentration on the ultrastructural appearance of bovine nasal cartilage proteoglycans.","authors":"P Front,&nbsp;C Dauguet,&nbsp;D R Mitrovic","doi":"10.3109/10520298909106983","DOIUrl":"https://doi.org/10.3109/10520298909106983","url":null,"abstract":"<p><p>Bovine nasal cartilage proteoglycan monomers were studied by Kleinschmidt and Zahn's molecular spreading technique as modified by Rosenberg et al. By decreasing the cytochrome c concentration in the epiphase to 2 micrograms per 100 microliters we were able on nitrocellulose-coated grids routinely to obtain highly contrasted and well spread proteoglycan monomers with a characteristic brush-like appearance and, sometimes, a clearly distinguishable hyaluronic acid binding region. Previously, a hyaluronic acid binding region has only been observed routinely in spread proteoglycan aggregates, and a brush-like structure of proteoglycan monomers on carbon-coated grids, but with considerably less precision due to the poor contrast of the molecules. Molecular spreading was further improved by decreasing the cytochrome c concentration in the epiphase to less than 2 micrograms per 100 microliters, but contrast was reduced making visualization of molecular details difficult.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 3","pages":"113-9"},"PeriodicalIF":0.0,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106983","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13699837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信