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Thiosemicarbazide used after periodic acid makes methenamine silver staining of renal glomerular basement membranes faster and cleaner. 经周期酸处理后使用硫代氨基脲可使肾小球基底膜甲基胺银染色更快、更干净。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106997
I Hayashi, Y Tome, Y Shimosato
{"title":"Thiosemicarbazide used after periodic acid makes methenamine silver staining of renal glomerular basement membranes faster and cleaner.","authors":"I Hayashi,&nbsp;Y Tome,&nbsp;Y Shimosato","doi":"10.3109/10520298909106997","DOIUrl":"https://doi.org/10.3109/10520298909106997","url":null,"abstract":"<p><p>The periodic acid-methenamine silver staining technique, which is frequently used for demonstrating the renal glomerular basement membrane, requires a high degree of skill, and in some cases it may be difficult to obtain a good result. To overcome such difficulty and inconsistency, we have improved the method by performing methenamine silver staining after oxidation with periodic acid and subsequent application of thiosemicarbazide. In this procedure, this semicarbazide enhanced the reaction of methenamine silver with the glomerular basement membrane and the reaction was completed within a shorter time in comparison with the conventional method. This modification also eliminated any nonspecific reaction with the surface of the glass slide and the solution container and yielded excellent and reproducible results irrespective of the fixation method and material employed. It was also found to stain the renal glomerular basement membrane of rabbits, which is demonstrable only with difficulty by the conventional method.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"185-90"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Fluorescence of plastic embedded tissue sections after curcumin staining. 姜黄素染色后塑料包埋组织切片的荧光。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909107001
J C Stockert, P Del Castillo, P S Testillano, M C Risueño
{"title":"Fluorescence of plastic embedded tissue sections after curcumin staining.","authors":"J C Stockert,&nbsp;P Del Castillo,&nbsp;P S Testillano,&nbsp;M C Risueño","doi":"10.3109/10520298909107001","DOIUrl":"https://doi.org/10.3109/10520298909107001","url":null,"abstract":"The natural dye, curcumin (C.I. 75300) from turmeric, is obtained from the roots of Curcuma longa (Lillie 1977). Curcumin has scarcely been applied for histological work, and its fluorescence seems to have been overlooked. During the course of studies on fluorescent aluminum complexes (Del Castillo et al. 1987) we realized that this dye induces a green fluorescence of chromatin (Stockert et al. 1989). In this note we describe the fluorescence reaction of curcumin on semithin sections of olastic embedded tissues.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"207-9"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
A simple method for staining nuclei of mature and germinated maize pollen. 成熟和发芽玉米花粉核染色的简单方法。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106996
M T Chang, M G Neuffer
{"title":"A simple method for staining nuclei of mature and germinated maize pollen.","authors":"M T Chang,&nbsp;M G Neuffer","doi":"10.3109/10520298909106996","DOIUrl":"https://doi.org/10.3109/10520298909106996","url":null,"abstract":"<p><p>A sugar acetocarmine staining technique has been developed for staining the sperm and vegetative nucleus of mature and germinated maize pollen grains. This procedure is simple, stable and highly repeatable. The physiological properties of the mature maize pollen grains are first adjusted by using an in vitro germinating culture solution. This solution is 15% sucrose and contains 360 ppm calcium chloride dihydrate, and 120 ppm boric acid. One part fresh pollen grains is uniformly mixed with nine parts of the solution and left at room temperature for at least 5 hr. One part of this solution is then mixed with two parts of regular acetocarmine stain and left overnight. The color of this mixture is pinkish red or raspberry. The sugar in the mixture helps to increase color contrast between the pollen cytoplasm (light pink) and the nuclei (reddish purple), decreases the frequency of burst pollen, increases pollen expansion, stabilizes pollen figures and automatically seals the coverglass.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"181-4"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
The ultramicrotome superstage: a versatile aid for section manipulation. 超微组超级级:一个多功能的辅助切片操作。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106999
M A Gorycki
{"title":"The ultramicrotome superstage: a versatile aid for section manipulation.","authors":"M A Gorycki","doi":"10.3109/10520298909106999","DOIUrl":"https://doi.org/10.3109/10520298909106999","url":null,"abstract":"<p><p>The design and properties of a rigid, box-like device to be placed on the knife stage of a Porter-Blum MT-2 ultramicrotome are described. This \"superstage\" acts as a hand and tool rest, and a wind screen for the knife boat. The superstage easily permits tight rafts of ultrathin sections to be centrally located on grids. Additionally, it aids in placing 1 micron thick sections at the same relative location on glass slides to facilitate their examination in the light microscope.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"197-9"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106999","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13836276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new method for preparation of undecalcified bone sections. 一种制备未钙化骨切片的新方法。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909107000
D L Sterchi, J A Eurell
{"title":"A new method for preparation of undecalcified bone sections.","authors":"D L Sterchi,&nbsp;J A Eurell","doi":"10.3109/10520298909107000","DOIUrl":"https://doi.org/10.3109/10520298909107000","url":null,"abstract":"<p><p>A new method for preparation of sections of undecalcified bone is described. Samples of ovine bone were embedded in methylmethacrylate and thick-sectioned with a cutoff machine or commercial band saw. Composite slides were prepared by gluing white acrylic to glass using cyanoacrylate glue. Bone sections were glued to the composite slide and then surface polished by grinding or ultramilling. The polished surface of the section was then etched and stained. The techniques described in this paper reduce the time spent grinding or milling sections and improve resolution of surface-stained features of undecalcified bone sections.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"201-5"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13836277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Assessment of demyelination in glycol methacrylate sections: a new protocol for cresyl fast violet staining. 甲基丙烯酸乙二醇切片脱髓鞘的评估:甲酰耐紫外染色的新方案。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106993
K B Nguyen, M P Pender
{"title":"Assessment of demyelination in glycol methacrylate sections: a new protocol for cresyl fast violet staining.","authors":"K B Nguyen,&nbsp;M P Pender","doi":"10.3109/10520298909106993","DOIUrl":"https://doi.org/10.3109/10520298909106993","url":null,"abstract":"<p><p>A simple staining technique for nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in glycol methacrylate. One-micrometer sections are stained with 0.05% cresyl fast violet aqueous solution at 60 C for 5 min, washed with tap water and air dried. With this method the details of all nervous tissue elements are clearly demonstrated. The technique is particularly useful for assessing demyelination because the staining of axoplasm allows demyelinated axons to be well visualized.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"163-7"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106993","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
High resolution techniques for study of human centromeric heterochromatin. 人类着丝异染色质研究的高分辨率技术。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106994
L Sanchez, V Goyanes
{"title":"High resolution techniques for study of human centromeric heterochromatin.","authors":"L Sanchez,&nbsp;V Goyanes","doi":"10.3109/10520298909106994","DOIUrl":"https://doi.org/10.3109/10520298909106994","url":null,"abstract":"<p><p>A high resolution procedure for analyzing human centromeric heterochromatin is described. The combined use of decondensation agents of pericentromeric heterochromatin and electron microscopy of whole mounted chromosomes allows a more precise identification of centromere and pericentromeric regions.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"169-74"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13764634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Optimal parameters for the histochemical demonstration of acetylcholinesterase in plastic sections of rat brain. 大鼠脑塑料切片乙酰胆碱酯酶组织化学研究的最佳参数。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106995
L R Williams, M R Donald, K S Jodelis
{"title":"Optimal parameters for the histochemical demonstration of acetylcholinesterase in plastic sections of rat brain.","authors":"L R Williams,&nbsp;M R Donald,&nbsp;K S Jodelis","doi":"10.3109/10520298909106995","DOIUrl":"https://doi.org/10.3109/10520298909106995","url":null,"abstract":"<p><p>A modified method for improved preservation and optical resolution of acetylcholinesterase (AChE)-containing structures in adult rat brain is described. Optimal tissue preparation included fixation in paraformaldehyde 4%, glutaraldehyde 0.1%, and sucrose 7% in 0.1M Sorensen's phosphate buffer, pH 7.4, rinsing in buffer 50 mM with respect to NH4Cl and 2% with respect to sucrose, acetone dehydration, vacuum infiltration with LKB Historesin, and polymerization at 4 C, overnight incubation of 10 microns sections at 37 C in the AChE histochemical reaction mixture and silver intensification according to Hedreen et al. Demonstration of AChE enzyme activity in the cholinergic projection from the rat basal forebrain to the ipsilateral hippocampus exemplifies the usefulness of the technique. The method provides an excellent demonstration of AChE-positive axonal processes and enables the pharmacohistochemical visualization of cholinergic neurons. This procedure offers a convenient method for analysis of cholinergic neurons that avoids potential artifacts inherent in other AChE histochemical procedures.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"175-80"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13764635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The use of gold chloride to stain developing and adult neuromuscular junctions in the insect. 用氯化金染色昆虫发育中的和成体的神经肌肉连接处。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106992
M B Rheuben, P J Schaner
{"title":"The use of gold chloride to stain developing and adult neuromuscular junctions in the insect.","authors":"M B Rheuben,&nbsp;P J Schaner","doi":"10.3109/10520298909106992","DOIUrl":"https://doi.org/10.3109/10520298909106992","url":null,"abstract":"<p><p>Individual insect muscle fibers, whose neuromuscular junctions have been stained with a modification of Ranvier's gold chloride method, can be dissected free and mounted whole if the muscle is prefixed in aldehydes. The neuromuscular junctions along the length of the individual fibers are well delineated and can be measured and counted. Effective procedures include fixation with glutaraldehyde buffered to low pH with sodium citrate, or glutaraldehyde and paraformaldehyde combined in phosphate buffer at neutral pH, followed by exposure to citric acid and to gold chloride. The method is convenient, and could be useful for the study of arthropod neuromuscular junctions in general, since their nerve terminals do not release acetylcholine as a transmitter and cannot be stained by the more commonly used cholinesterase methods.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"155-62"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106992","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Hoechst 33342 staining coupled with conventional histological technique. Hoechst 33342染色结合常规组织学技术。
Stain technology Pub Date : 1989-07-01 DOI: 10.3109/10520298909106998
W Sawicki, S Moskalewski
{"title":"Hoechst 33342 staining coupled with conventional histological technique.","authors":"W Sawicki,&nbsp;S Moskalewski","doi":"10.3109/10520298909106998","DOIUrl":"https://doi.org/10.3109/10520298909106998","url":null,"abstract":"<p><p>Hoechst 33342 was injected either intravenously or intraperitoneally into mice which were killed 1 or 24 hr or 7, 14 or 28 days later. Various organs were fixed and paraffin embedded. Visual inspection showed that independently of the route of dye administration or survival time, distinct fluorescence of nuclei was observed in organs other than cerebral cortex. Even formic acid decalcification of bone failed to abolish the fluorescence of osteocytes. In vivo staining with Hoechst 33342 is proposed as an alternative for staining after sectioning. Cells from spleens of Hoechst 33342-injected mice or stained in vitro were injected intramuscularly into mice. Hoechst 33342-stained splenocytes could be found in deparaffinized sections at the site of injection 24 hr later.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 4","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909106998","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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