一种制备未钙化骨切片的新方法。

D L Sterchi, J A Eurell
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引用次数: 29

摘要

介绍了一种制备非钙化骨切片的新方法。将羊骨样品包埋在甲基丙烯酸甲酯中,并用切断机或商用带锯进行厚切片。采用氰基丙烯酸酯胶将白色丙烯酸树脂粘接到玻璃上,制备复合玻片。骨段粘在复合载玻片上,然后通过研磨或超磨抛光表面。然后对该部分的抛光表面进行蚀刻和染色。本文描述的技术减少了研磨或研磨切片所花费的时间,并提高了未钙化骨切片表面染色特征的分辨率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A new method for preparation of undecalcified bone sections.

A new method for preparation of sections of undecalcified bone is described. Samples of ovine bone were embedded in methylmethacrylate and thick-sectioned with a cutoff machine or commercial band saw. Composite slides were prepared by gluing white acrylic to glass using cyanoacrylate glue. Bone sections were glued to the composite slide and then surface polished by grinding or ultramilling. The polished surface of the section was then etched and stained. The techniques described in this paper reduce the time spent grinding or milling sections and improve resolution of surface-stained features of undecalcified bone sections.

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