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Simple, reliable detection of latex microspheres in high quality tissue sections. 简单，可靠的检测乳胶微球在高质量的组织切片。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107010
K S Pierce, D C Morfitt
{"title":"Simple, reliable detection of latex microspheres in high quality tissue sections.","authors":"K S Pierce, D C Morfitt","doi":"10.3109/10520298909107010","DOIUrl":"https://doi.org/10.3109/10520298909107010","url":null,"abstract":"Latex microspheres used in biological research have been visualized by light microscopy in mounts of cell suspensions, disrupted cells, or cleared tissues (Mishima et al 1987, Koonce et al 1986, LeFevre et al 1978); in unembedded coverslip monolayers (Koerten et al 1980); in fixed (Cornwall and Phillipson 1988) or unfixed (Wells et al 1988) frozen sections; in paraffin sections cleared and deparaffinized with n-butyl alcohol (Callebaut and Meeussen 1989); and in tissues embedded in resins suitable for transmission electron microscopy, such as Spurr's (Hampton et al 1987), Epon (Herzog and Miller 1979), or Ladd Low Viscosity Epon (LeFevre et al 1985). Paraffin embedding, and some plastic embedments, are impractical for demonstration of latex beads because the beads are dissolved by such organic solvents as xylene, dioxane, or chloroform (Van Furth and Diesselhoff-Den Dulk 1980), propylene oxide (Lentzen et al 1984), amyl acetate (Okada et al 1981), or toluene, the solvent in commonly used mounting media su...","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"249-51"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
A method for flat embedding thick cryostat tissue sections in plastic resin. 一种在塑料树脂中平埋低温恒温器厚组织切片的方法。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107015
M B Parr, S A Hunter, E L Parr
{"title":"A method for flat embedding thick cryostat tissue sections in plastic resin.","authors":"M B Parr, S A Hunter, E L Parr","doi":"10.3109/10520298909107015","DOIUrl":"https://doi.org/10.3109/10520298909107015","url":null,"abstract":"In many histochemical procedures tissues are often sectioned at 30-50 nm (thick sections) to maximize penetration of the substrate into the tissues. After the histochemical reaction the sections can be embedded in plastic resins for thin sectioning and transmission electron microscopy study. Recently, we have used one such procedure to study endocytosis of horseradish peroxidase into Langerhans cells in the murine vaginal epithelium (Parr and Parr 1990). This enzyme has been used extensively as a protein tracer in biologic studies because it can be localized by a simple histochemical procedure for both light and electron microscopy (Graham and Karnovsky 1966). In carrying out the histochemical procedure we encountered problems embedding the thick cryostat sections because they became coiled and twisted during processing. In this report we describe a simple technique for flat embedding thick cryostat sections in plastic resin.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"261-3"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13774948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Standardized thionin-eosin Y: a quick stain for cytology. 标准硫蛋白-伊红Y:细胞学快速染色。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107012
E K Schulte, D H Wittekind
{"title":"Standardized thionin-eosin Y: a quick stain for cytology.","authors":"E K Schulte, D H Wittekind","doi":"10.3109/10520298909107012","DOIUrl":"https://doi.org/10.3109/10520298909107012","url":null,"abstract":"Two stains long used in exfoliative cytology, the hematoxylin-eosin Y and Papanicolaou stains, have not been standardized even today. Some dozens of hematoxylin and eosin and Papanicolaou staining recipes have been recommended in the literature. Consequently, the staining pattern of hematoxylin and eosin, and Papanicolaou stained cytological material varies from laboratory to laboratory. To a certain degree this is due to batch-to-batch variations of commercial samples of the natural dye hematoxylin (C.I. 75290). The present paper describes a simple, standardized and reproducible procedure using thionin bromide to replace hematoxylin in the hematoxylin and eosin stain.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"255-6"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
The session of the 1989 annual meeting of the Biological Stain Commission held at Rochester, New York: a personal view. 1989年在纽约罗切斯特举行的生物污渍委员会年会:个人观点。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107017
R W Horobin
{"title":"The session of the 1989 annual meeting of the Biological Stain Commission held at Rochester, New York: a personal view.","authors":"R W Horobin","doi":"10.3109/10520298909107017","DOIUrl":"https://doi.org/10.3109/10520298909107017","url":null,"abstract":"","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"269-71"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elimination or reduction of wrinkles in semithin epoxy sections by vacuum drying. 通过真空干燥消除或减少半薄环氧树脂部分的皱纹。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107004
H H Crowley, B H Leichtling
{"title":"Elimination or reduction of wrinkles in semithin epoxy sections by vacuum drying.","authors":"H H Crowley,&nbsp;B H Leichtling","doi":"10.3109/10520298909107004","DOIUrl":"https://doi.org/10.3109/10520298909107004","url":null,"abstract":"<p><p>Vacuum drying, under appropriate conditions, diminishes the warping and buckling of epoxy semithin sections and enhances visualization with light microscopy. Treatment of sections with chloroform or variations in the drying times or temperatures did not reduce wrinkling.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"221-3"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A quick and standardized giemsa stain for wet-fixed cytological material. 湿固定细胞学材料的快速标准化吉姆萨染色方法。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107011
E K Schulte, D H Wittekind
{"title":"A quick and standardized giemsa stain for wet-fixed cytological material.","authors":"E K Schulte,&nbsp;D H Wittekind","doi":"10.3109/10520298909107011","DOIUrl":"https://doi.org/10.3109/10520298909107011","url":null,"abstract":"The Giemsa stain is one of the most widely used staining techniques in cytology, especially in hematology. A standardized Romanowsky-Giemsa staining procedure using pure cationic azure B (C.I. 52010) and anionic eosin (C.I. 45380) has been described by Wittekind et al (1982). A revised standard Giemsa staining procedure was recently published (Wittekind and Kretschmer 1987). Usually the Romanowsky-Giemsa stain is applied to air dried and methanol fixed cytological material, e.g. blood smears and bone marrow films (ICSH 1984).","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"253-4"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Processing of free-living amoebae for transmission electron microscopy. 用于透射电子显微镜的自由生活变形虫的加工。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107014
I A Niszl, M B Markus
{"title":"Processing of free-living amoebae for transmission electron microscopy.","authors":"I A Niszl,&nbsp;M B Markus","doi":"10.3109/10520298909107014","DOIUrl":"https://doi.org/10.3109/10520298909107014","url":null,"abstract":"Ultrastructural features have proved useful in identifying and classifying free-living amoebae (Page 1985). A simple technic which facilitates embedding amoebae for transmission electron microscoov is described here.","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"259-60"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13774947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Technical considerations in evaluating the adhesion of leukocytes to aortic endothelium of the rat. 评价白细胞与大鼠主动脉内皮黏附的技术考虑。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107003
M Gabaldón, C Capdevila
{"title":"Technical considerations in evaluating the adhesion of leukocytes to aortic endothelium of the rat.","authors":"M Gabaldón,&nbsp;C Capdevila","doi":"10.3109/10520298909107003","DOIUrl":"https://doi.org/10.3109/10520298909107003","url":null,"abstract":"<p><p>Comments on techniques for characterizing leukocytes adhered to the aortic endothelium of the rat are given. Alpha-naphthyl acetate esterase positive leukocytes were studied by optical microscopy of en face intima-media preparations. Results indicate 1) 1% paraformaldehyde-2% glutaraldehyde is a better fixative than formalin-calcium or 4% paraformaldehyde with or without 1.5 mM CaCl2; the latter produces distortion of leukocytes, endothelial desquamation and enzymate inhibition, 2) washing the aorta with phosphate-buffered saline for 90 sec prior to fixation-perfusion produces a notable decrease in the number of leukocytes adhered, 3) diazotized parasaniline is better than fast blue RR salt as coupling agent in the esterase reaction, and 4) counterstaining with 1% methyl green for 1 min, before or after the esterase reaction, is not adequate because of limited contrast and the heavy staining of smooth muscle. Counterstaining with Gill's hematoxylin No. 3 for 90 sec is adequate only when done before the esterase reaction. Inhibition of endothelial esterase activity by hematoxylin decreases background, favors contrast of adhered leukocytes and makes it possible to observe nucleus-cytoplasm relations.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"211-9"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Observing transmitting tissue and other structures in the pistil using a fluorescent stain. 用荧光染色观察雌蕊中的透射组织和其他结构。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107006
L J Glicenstein, R Craig
{"title":"Observing transmitting tissue and other structures in the pistil using a fluorescent stain.","authors":"L J Glicenstein,&nbsp;R Craig","doi":"10.3109/10520298909107006","DOIUrl":"https://doi.org/10.3109/10520298909107006","url":null,"abstract":"<p><p>Using safranin O as a fluorescent stain at a wavelength range of 355-425 nm has allowed us to distinguish the transmitting tissue and the nature of this tissue in pistils of members of the Geraniaceae and Gentianaceae. Xylem, amyloplasts, and high tannin containing tissues, such as mericarp walls, were also readily differentiated.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"229-31"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An improved method for studying grass leaf epidermis. 一种改进的草叶表皮研究方法。
Stain technology Pub Date : 1989-09-01 DOI: 10.3109/10520298909107008
J D Theunissen
{"title":"An improved method for studying grass leaf epidermis.","authors":"J D Theunissen","doi":"10.3109/10520298909107008","DOIUrl":"https://doi.org/10.3109/10520298909107008","url":null,"abstract":"<p><p>Leaf epidermis of grasses is elaborate and important in the systematics of the Poaceae at subfamily and genus level. Most available techniques used in preparing leaf epidermis for microscopic studies are time-consuming and often produce preparations inadequate for studying histological detail. A combination of the hand scraping and maceration methods with modifications is proposed in this paper to prepare epidermal peels comparatively rapidly. One epidermal layer was scraped off and the mesophyll tissue removed from the epidermis to be studied by maceration in HNO3. The recovered epidermal peel was neutralized in NaOH and stained with malachite green or safranin O. Preparations made by this technique are suitable for studies of epidermal features, measurements of special structures and determinations of trichome indices. This method has been used in a study investigating intraspecific variation in southern African pasture grasses.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"64 5","pages":"239-42"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520298909107008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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