一种多功能的新矿化骨染色剂,用于同时评估四环素和类骨接缝。

A R Villanueva, K D Lundin
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引用次数: 98

摘要

开发了一种多功能矿化骨染色(MIBS),用于同时在薄或厚未钙化切片上显示类骨接缝和四环素荧光。骨标本固定在70%的乙醇中,但10%的缓冲福尔马林是允许的。根据个人喜好,这些标本可以不染色,也可以在塑料包埋前进行染色。骨样接缝呈绿色至翡翠绿色,或浅至深紫色。矿化后的骨基质无色或呈绿色。成骨细胞和破骨细胞细胞核为浅紫色至深紫色,细胞质为浅灰色至粉红色。用这种方法鉴定类骨缝与用同一活检组织的同一切片进行体内四环素摄取鉴定非常一致。该方法显示晕状体积,紫色为活骨细胞周围的异常腔隙低密度骨。这种现象常见于维生素d抗性佝偻病、氟中毒、肾性骨营养不良、甲状旁腺功能亢进,有时也见于氟化物治疗的骨质疏松症患者。在骨软化性骨中,由于矿化片之间存在未矿化的类骨,大多数类骨接缝呈不规则染色。该方法已在羟基磷灰石种植体和骨移植体的新骨形成染色中得到了有效的应用。旧的,未染色的,塑料嵌入的未钙化的部分被染色,以及在去除盖盖后的新鲜部分。该染色也有望从重塑、组织形态学和病理学的角度研究不同的代谢性骨疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A versatile new mineralized bone stain for simultaneous assessment of tetracycline and osteoid seams.

A versatile mineralized bone stain (MIBS) for demonstrating osteoid seams and tetracycline fluorescence simultaneously in thin or thick undecalcified sections has been developed. Bone specimens are fixed in 70% ethanol, but 10% buffered formalin is permissible. Depending upon one's preference, these specimens can be left unstained or be prestained before plastic embedding. Osteoid seams are stained green to jade green, or light to dark purple. Mineralized bone matrix is unstained or green. Osteoblast and osteoclast nuclei are light to dark purple, cytoplasm varies from slightly gray to pink. The identification of osteoid seams by this method agrees closely with identification by in vivo tetracycline uptake using the same section from the same biopsy. The method demonstrates halo volumes, an abnormal, lacunar, low density bone around viable osteocytes in purple. This phenomenon is commonly seen in vitamin D-resistant rickets, fluorosis, renal osteodystrophy, hyperparathyroidism, and is sometimes seen in fluoride treated osteoporotic patients. In osteomalacic bone, most osteoid seams are irregularly stained as indicated by the presence of unmineralized osteoid between mineralized lamellae. The method has been used effectively in staining new bone formation in hydroxyapatite implants and bone grafts. Old, unstained, plastic embedded undecalcified sections are stained as well as fresh sections after removal of the coverslip. This stain also promises to be valuable in the study of different metabolic bone diseases from the point of view of remodeling, histomorphometry, and pathology.

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