啮齿动物胚胎组织的固定物评价和组织学外观。

Stain technology Pub Date : 1989-01-01 DOI:10.3109/10520298909108036

W B Howard, C C Willhite, R A Smart

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引用次数: 3

摘要

采用Zenker溶液、含酒精福尔马林、Bouin液、10%中性缓冲福尔马林或3%戊二醛固定，苏木精和伊红染色，对早期仓鼠胚胎进行组织病理学研究。与其他候选处理技术相比，在Zenker氏液中固定，然后在中性缓冲福尔马林中固定，可以更好地保存正常胚胎亚细胞的细节。

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Fixative evaluation and histologic appearance of embryonic rodent tissue.

Histopathologic study of early hamster embryos was carried out after fixation in Zenker's solution, alcoholic formalin, Bouin's fluid, 10% neutral buffered formalin, or 3% glutaraldehyde and staining with hematoxylin and eosin. Fixation in Zenker's fluid followed by postfixation in neutral buffered formalin provided superior preservation of normal embryonic subcellular detail as compared to the other candidate processing techniques.

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来源期刊

Stain technology

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