一种利用树脂萃取的塑料包埋材料提高荧光显微镜分辨率的方法。

Stain technology Pub Date : 1990-01-01 DOI:10.3109/10520299009105616

S D Russell

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引用次数: 3

摘要

给出了用氢氧化钠、苯、丙酮和甲醇从半薄切片中提取环氧树脂的方案。这种切片在荧光显微镜观察中优于石蜡或未切片的材料。使用ISO 3200的超快胶片(例如，柯达T-Max P3200)，在不使用抗褪色剂和不引入不可接受的照相颗粒尺寸的情况下，最大限度地减少褪色。

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A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.

A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.

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Stain technology

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