{"title":"抑制再激活肌纤维atp酶技术在一个单一的低温冷冻肌肉切片中显示三种纤维类型。","authors":"V Horák, S Matolín","doi":"10.3109/10520299009108061","DOIUrl":null,"url":null,"abstract":"<p><p>An inhibition reactivation technique was used for histochemical staining of human skeletal muscle sections. Myofibrillar ATPase activity was inhibited by sodium hydroxymercuribenzoate (2.5 mM in 0.1 M Tris-HCl buffer, pH 7.2-7.5, 30 min) and successively reactivated by cysteine which was added to incubation solution (10 mM cysteine-HCl, 2.5 mM ATP-disodium salt, 50 mM potassium chloride and 27 mM calcium chloride in barbital buffer, pH 9.4, 35 min at 37 C). This technique allows the distinction of three fiber categories with different staining intensities in single cross-section. Dark, intermediate and light fibers correspond to IIB, I, and IIA types, respectively. Storage of air dried sections in the freezer at -20 C for one month had no influence on staining characteristics.</p>","PeriodicalId":21924,"journal":{"name":"Stain technology","volume":"65 2","pages":"85-9"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10520299009108061","citationCount":"4","resultStr":"{\"title\":\"Inhibition reactivation myofibrillar ATPase technique for demonstration of three fiber types in a single cryostat muscle section.\",\"authors\":\"V Horák, S Matolín\",\"doi\":\"10.3109/10520299009108061\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An inhibition reactivation technique was used for histochemical staining of human skeletal muscle sections. Myofibrillar ATPase activity was inhibited by sodium hydroxymercuribenzoate (2.5 mM in 0.1 M Tris-HCl buffer, pH 7.2-7.5, 30 min) and successively reactivated by cysteine which was added to incubation solution (10 mM cysteine-HCl, 2.5 mM ATP-disodium salt, 50 mM potassium chloride and 27 mM calcium chloride in barbital buffer, pH 9.4, 35 min at 37 C). This technique allows the distinction of three fiber categories with different staining intensities in single cross-section. Dark, intermediate and light fibers correspond to IIB, I, and IIA types, respectively. Storage of air dried sections in the freezer at -20 C for one month had no influence on staining characteristics.</p>\",\"PeriodicalId\":21924,\"journal\":{\"name\":\"Stain technology\",\"volume\":\"65 2\",\"pages\":\"85-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/10520299009108061\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Stain technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10520299009108061\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stain technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10520299009108061","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
采用抑制再激活技术对人体骨骼肌切片进行组织化学染色。肌纤维atp酶活性被羟汞苯甲酸钠(2.5 mM在0.1 M Tris-HCl缓冲液中,pH 7.2-7.5, 30分钟)抑制,随后被半胱氨酸(10 mM半胱氨酸- hcl, 2.5 mM atp -二钠盐,50 mM氯化钾和27 mM氯化钙在巴比土缓冲液中,pH 9.4, 35分钟,37℃)重新激活。该技术允许在单个横剖面上区分具有不同染色强度的三种纤维。暗光纤、中光纤和光光纤分别对应IIB、I和IIA类型。风干切片在-20℃的冷冻室中保存一个月对染色特性没有影响。
Inhibition reactivation myofibrillar ATPase technique for demonstration of three fiber types in a single cryostat muscle section.
An inhibition reactivation technique was used for histochemical staining of human skeletal muscle sections. Myofibrillar ATPase activity was inhibited by sodium hydroxymercuribenzoate (2.5 mM in 0.1 M Tris-HCl buffer, pH 7.2-7.5, 30 min) and successively reactivated by cysteine which was added to incubation solution (10 mM cysteine-HCl, 2.5 mM ATP-disodium salt, 50 mM potassium chloride and 27 mM calcium chloride in barbital buffer, pH 9.4, 35 min at 37 C). This technique allows the distinction of three fiber categories with different staining intensities in single cross-section. Dark, intermediate and light fibers correspond to IIB, I, and IIA types, respectively. Storage of air dried sections in the freezer at -20 C for one month had no influence on staining characteristics.