Stem Cell Reports最新文献_第8页

Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research. 促进采用最佳做法和标准，以提高干细胞研究的质量和可重复性。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-06-12 DOI: 10.1016/j.stemcr.2025.102531

Lucia Selfa Aspiroz, Milena Mennecozzi, Laura Batlle, Barbara Corneo, Lyn Healy, Mark Kotter, Andreas Kurtz, Tenneille E Ludwig, Christine Mummery, Martin Pera, Glyn N Stacey, Carlos A Tristan, Maurice Whelan

引用次数: 0

Biomanufacturing in low Earth orbit: A paradigm shift. 近地轨道生物制造：范式转变。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-06-19 DOI: 10.1016/j.stemcr.2025.102536

Davide Marotta, Noor Ward, Steven R Bauer, Joshua Hunsberger, Jana Stoudemire, Kenneth Savin, Marc Giulianotti, Catriona H M Jamieson, Donna Roberts, Michael Roberts

引用次数: 0

Graft-derived horizontal cells contribute to host-graft synapses in degenerated retinas after retinal organoid transplantation. 在视网膜类器官移植后变性视网膜中，移植物来源的水平细胞有助于宿主-移植物突触的形成。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-06-26 DOI: 10.1016/j.stemcr.2025.102545

Mikiya Watanabe, Takayuki Yamada, Hung-Ya Tu, Taro Chaya, Satoko Okayama, Kenta Onoue, Shigenobu Yonemura, Chieko Koike, Masao Tachibana, Takahisa Furukawa, Michiko Mandai

{"title":"Graft-derived horizontal cells contribute to host-graft synapses in degenerated retinas after retinal organoid transplantation.","authors":"Mikiya Watanabe, Takayuki Yamada, Hung-Ya Tu, Taro Chaya, Satoko Okayama, Kenta Onoue, Shigenobu Yonemura, Chieko Koike, Masao Tachibana, Takahisa Furukawa, Michiko Mandai","doi":"10.1016/j.stemcr.2025.102545","DOIUrl":"10.1016/j.stemcr.2025.102545","url":null,"abstract":"Stem cell-derived retinal organoid (RO) transplantation is a promising approach for treating retinal degenerative diseases such as retinitis pigmentosa. Photoreceptors (PRs) in RO sheets form synaptic connections with host bipolar cells (BCs) and improve visual function in animal models of advanced retinal degeneration. However, the contribution of horizontal cells (HCs), which are critical for PR synapse formation in the developing retina, to host-graft synapse formation remains unclear. In this study, we used HC-depleted retinal degeneration mice (rd1) and Islet1-/- genome-edited ROs (gROs) that contain HCs but not rod BCs and showed that host HC deficiency did not alter the number of host BC-graft PR synapses after transplantation into rd1, while the restored light sensitivity was enhanced in the context of host HCs. These findings indicate that graft-derived HCs alone can support host-graft synapse formation, whereas the presence of host HCs facilitates functional recovery after gRO transplantation.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102545"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ins and outs of HSCs and VEGF-induced vascular remodeling: Toward improved HSC mobilization and engraftment. 造血干细胞和vegf诱导的血管重塑：促进造血干细胞的动员和植入。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 DOI: 10.1016/j.stemcr.2025.102569

Madeline J Caduc, Simón Méndez-Ferrer

引用次数: 0

Neuroinflammation and EIF2 Signaling Persist despite Antiretroviral Treatment in an hiPSC Tri-culture Model of HIV Infection. 在HIV感染的hiPSC三培养模型中，尽管抗逆转录病毒治疗，神经炎症和EIF2信号仍然存在。

IF 5.1 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-06-04 DOI: 10.1016/j.stemcr.2025.102549

Sean K Ryan, Michael V Gonzalez, James P Garifallou, Frederick C Bennett, Kimberly S Williams, Nathaniel P Sotuyo, Eugene Mironets, Kieona Cook, Hakon Hakonarson, Stewart A Anderson, Kelly L Jordan-Sciutto

引用次数: 0

BMP, MEK, and WNT inhibition with NGN2 expression for rapid generation of hiPSC-derived neurons amenable to regional patterning. 抑制BMP、MEK和WNT与NGN2的表达，快速生成符合区域模式的hipsc衍生神经元。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-06-19 DOI: 10.1016/j.stemcr.2025.102539

Carina Habich, Alexandra Kowalski, Astrid Wachter, Michaela J Heimann, Michael Wolf, Markus P Kummer, Nathalie Nicolaisen, Christopher Sliwinski, Lydia Reinhardt, Veronika Heil, Timo Lange, Christopher Untucht, Loan N Miller, Jürgen Korffmann, Daniela Geist, David Schöndorf, Heyne Lee, Lamiaa Bahnassawy, Benjamin Mielich-Süss, Melanie S Brennan, Ruven Wilkens, Julian Röwe, Ian Weidling, Rüdiger Rudolf, Mathias Hafner, Justine D Manos, Miroslav Cik, Peter Reinhardt

{"title":"BMP, MEK, and WNT inhibition with NGN2 expression for rapid generation of hiPSC-derived neurons amenable to regional patterning.","authors":"Carina Habich, Alexandra Kowalski, Astrid Wachter, Michaela J Heimann, Michael Wolf, Markus P Kummer, Nathalie Nicolaisen, Christopher Sliwinski, Lydia Reinhardt, Veronika Heil, Timo Lange, Christopher Untucht, Loan N Miller, Jürgen Korffmann, Daniela Geist, David Schöndorf, Heyne Lee, Lamiaa Bahnassawy, Benjamin Mielich-Süss, Melanie S Brennan, Ruven Wilkens, Julian Röwe, Ian Weidling, Rüdiger Rudolf, Mathias Hafner, Justine D Manos, Miroslav Cik, Peter Reinhardt","doi":"10.1016/j.stemcr.2025.102539","DOIUrl":"10.1016/j.stemcr.2025.102539","url":null,"abstract":"Human induced pluripotent stem cells (hiPSCs) are a promising tool for studying neurological diseases and developing therapies for neurodegenerative diseases. Differentiation of hiPSCs into neurons can be achieved by dual SMAD inhibition (dSMADi) or by induced neurogenin 2 (NGN2) overexpression (\"iNGN2\"). Starting directly from hiPSCs, iNGN2 shortens the time to a neuronal stage but leads to neurons partially resembling peripheral or posterior fates while dSMADi more faithfully recapitulates telencephalic development. To modify the iNGN2 approach, we applied an accelerated induction paradigm that is dependent on the inhibition of BMP, MEK, and WNT pathways (\"BMWi\"), to commit hiPSCs into a telencephalic fate before iNGN2. The resulting neurons showed strong expression of telencephalic markers, with decreased levels of peripheral and posterior marker genes compared to iNGN2 alone. The resulting telencephalic neurons are suitable for a tau aggregation assay. Furthermore, we could demonstrate that during BMWi treatment, the cells are amenable to additional regional patterning cues. This allowed the generation of neurons from different regions of the CNS and peripheral nervous system (PNS), which will significantly facilitate in vitro modeling of a range of neurodevelopmental and neurodegenerative disorders.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102539"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patient-specific and gene-corrected induced pluripotent stem cell-derived endothelial cells elucidate single-cell phenotype of pulmonary veno-occlusive disease. 患者特异性和基因校正诱导多能干细胞衍生的内皮细胞阐明了肺静脉闭塞疾病的单细胞表型。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-07-08 Epub Date: 2025-05-28 DOI: 10.1016/j.stemcr.2025.102540

Baihui Ma, Tianjiao Li, Wenke Li, Hang Yang, Qixian Zeng, Zihang Pan, Kai Wang, Qianlong Chen, Changming Xiong, Zhou Zhou

引用次数: 0

Two types of regenerative cell populations appear in acute liver injury. 两种类型的再生细胞群出现在急性肝损伤。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-08 DOI: 10.1016/j.stemcr.2025.102503

Tomomi Aoyagi, Takeshi Goya, Koji Imoto, Yuki Azuma, Tomonobu Hioki, Motoyuki Kohjima, Masatake Tanaka, Yoshinao Oda, Yoshihiro Ogawa

{"title":"Two types of regenerative cell populations appear in acute liver injury.","authors":"Tomomi Aoyagi, Takeshi Goya, Koji Imoto, Yuki Azuma, Tomonobu Hioki, Motoyuki Kohjima, Masatake Tanaka, Yoshinao Oda, Yoshihiro Ogawa","doi":"10.1016/j.stemcr.2025.102503","DOIUrl":"10.1016/j.stemcr.2025.102503","url":null,"abstract":"The liver has a robust regenerative capacity. However, the mechanisms underlying this process remain unclear. Numerous studies on liver regeneration have been previously conducted using partial hepatectomy models, which may not fully represent acute liver injury with inflammation and necrosis. This is commonly observed in the majority of clinical cases. In this study, we conducted a single-cell RNA sequencing (RNA-seq) analysis of liver regeneration in acetaminophen-treated mice using publicly available data. We found that two distinct populations of regenerative cells simultaneously appeared within the same regenerative process. The two populations significantly differed in terms of cell morphology, differentiation, localization, proliferation rate, and signal response. Moreover, one of the populations was induced by contact with necrotic tissue and demonstrated a higher proliferative capacity with a dedifferentiated feature. These findings provide new insights into liver regeneration and therapeutic strategies for liver failure.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102503"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and lineage tracing of a mouse adipose depot reveal properties conserved with human supraclavicular brown adipose tissue. 小鼠脂肪库的表征和谱系追踪揭示了人类锁骨上棕色脂肪组织的保守特性。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-22 DOI: 10.1016/j.stemcr.2025.102509

Liang Li, Brian J Feldman

引用次数: 0

Stem cell-based embryo models: The 2021 ISSCR stem cell guidelines revisited. 基于干细胞的胚胎模型：2021年ISSCR干细胞指南重新审视。

IF 5.1 2区医学

Stem Cell Reports Pub Date : 2025-06-10 DOI: 10.1016/j.stemcr.2025.102514

Amander T Clark, Heidi Cook-Andersen, Sarah Franklin, Rosario Isasi, Debra J H Mathews, Vincent Pasque, Peter J Rugg-Gunn, Patrick P L Tam, Hongmei Wang, Jan J Zylicz, Janet Rossant

{"title":"Stem cell-based embryo models: The 2021 ISSCR stem cell guidelines revisited.","authors":"Amander T Clark, Heidi Cook-Andersen, Sarah Franklin, Rosario Isasi, Debra J H Mathews, Vincent Pasque, Peter J Rugg-Gunn, Patrick P L Tam, Hongmei Wang, Jan J Zylicz, Janet Rossant","doi":"10.1016/j.stemcr.2025.102514","DOIUrl":"10.1016/j.stemcr.2025.102514","url":null,"abstract":"Human stem cell-based embryo models (SCBEMs) are a research technology with the potential to facilitate our understanding of human embryogenesis, improve assisted reproductive technology outcomes, elucidate the causes of early pregnancy failure, and provide a clearer understanding of the developmental origins of disease. Given that human SCBEMs are designed to model specific phenotypic features and developmental processes of human embryos, they raise distinct concerns from other stem cell models, such as organoids. The International Society for Stem Cell Research (ISSCR) Guidelines for Stem Cell Research and Clinical Translation, published in 2021, made recommendations for research oversight of SCBEMs and established different categories of review based on involvement of embryonic and extraembryonic lineages. However, recent progress has enabled unexpected ways to create increasingly complex models, as well as more efficient means of doing so without including all major extraembryonic lineages. A working group was tasked by the ISSCR executive to undertake a thorough reexamination of the guidelines in the light of these advances. The three main recommendations of the working group are that all research involving organized 3-dimensional human SCBEMs (1) should be subject to appropriate review, (2) must have a clear scientific rationale, and (3) must be subject to limited timelines. The proposed modifications to the ISSCR guidelines are intended to bring more clarity to the field, help guide the deliberations of researchers, oversight committees and other relevant stakeholders, and ensure continued public confidence.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":"20 6","pages":"102514"},"PeriodicalIF":5.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0