Stem Cell Reports最新文献

A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-27 DOI: 10.1016/j.stemcr.2025.102446

Iwona Szczerbinska, Kevin Andrew Uy Gonzales, Engin Cukuroglu, Muhammad Nadzim Bin Ramli, Bertha Pei Ge Lee, Cheng Peow Tan, Cheng Kit Wong, Giulia Irene Rancati, Hongqing Liang, Jonathan Göke, Huck-Hui Ng, Yun-Shen Chan

引用次数: 0

Safe CNV removal is crucial for successful hESC-RPE transplantation in wet age-related macular degeneration.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-20 DOI: 10.1016/j.stemcr.2025.102424

Ying Xue Lv, Qi You Li, Ping Duan, Min Fang Zhang, Bo Liu, Shi Ying Li, Tong Tao Zhao, Hao Wang, Yong Liu, Zheng Qin Yin

{"title":"Safe CNV removal is crucial for successful hESC-RPE transplantation in wet age-related macular degeneration.","authors":"Ying Xue Lv, Qi You Li, Ping Duan, Min Fang Zhang, Bo Liu, Shi Ying Li, Tong Tao Zhao, Hao Wang, Yong Liu, Zheng Qin Yin","doi":"10.1016/j.stemcr.2025.102424","DOIUrl":"https://doi.org/10.1016/j.stemcr.2025.102424","url":null,"abstract":"Subretinal transplantation of human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells has demonstrated therapeutic potential in macular degeneration. However, its efficiency is limited in wet age-related macular degeneration (wet AMD) due to choroidal neovascularization (CNV). To investigate the feasibility of hESC-RPE cell transplantation, we employed a surgical approach to induce retinal detachment, which allowed the removal of CNV lesions. After retinal reattachment, hESC-RPE cells were transplanted into the subretinal space. Ten patients were enrolled and divided into 2 groups. No retinal edema or CNV recurrence was observed in group 1 (7 patients without bleeding). Group 2 (3 patients with bleeding) had persistent fundus inflammation, and one patient experienced CNV recurrence. All patients were managed effectively without vision loss. These findings suggest that subretinal transplantation of hESC-RPE cells after CNV removal is safe and well tolerated; however, damage caused during CNV removal may trigger persistent inflammation and CNV recurrence. This study was registered at ClinicalTrials.gov (NCT02749734).","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102424"},"PeriodicalIF":5.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-dose radiation preferentially induces the clonal expansion of hematopoietic progenitor cells over mature T and B cells in mouse bone marrow.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-20 DOI: 10.1016/j.stemcr.2025.102423

Kengo Yoshida, Munechika Misumi, Kanya Hamasaki, Seishi Kyoizumi, Yasunari Satoh, Tatsuaki Tsuruyama, Arikuni Uchimura, Yoichiro Kusunoki

{"title":"High-dose radiation preferentially induces the clonal expansion of hematopoietic progenitor cells over mature T and B cells in mouse bone marrow.","authors":"Kengo Yoshida, Munechika Misumi, Kanya Hamasaki, Seishi Kyoizumi, Yasunari Satoh, Tatsuaki Tsuruyama, Arikuni Uchimura, Yoichiro Kusunoki","doi":"10.1016/j.stemcr.2025.102423","DOIUrl":"https://doi.org/10.1016/j.stemcr.2025.102423","url":null,"abstract":"Radiation induces clonal hematopoiesis (CH) involving high-frequency somatic mutations in hematopoietic cells. However, the effects of radiation on clonal expansion of hematopoietic progenitor cells and lymphocytes remain elusive. Here, we investigate CH mutations and T cell receptor (TCR) and B cell receptor (BCR) sequences within the bone marrow cells of mice 18 months after irradiation (3 Gy) and age-matched controls. Two to six CH mutations were identified in the irradiated mice (N = 5), while only one of the four control mice carried a CH mutation. These CH mutations detected in the bone marrow were also identified in the splenic CD11b+ myeloid cell population. Meanwhile, the cumulative size of the ten largest TCR and BCR clones, as well as their clonality, did not differ significantly between irradiated and control mice. Our findings suggest that radiation preferentially induces clonal expansion of hematopoietic progenitor cells over mature lymphocytes in the bone marrow.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102423"},"PeriodicalIF":5.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac differentiation roadmap for analysis of plasticity and balanced lineage commitment.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-20 DOI: 10.1016/j.stemcr.2025.102422

Rebecca R Snabel, Carla Cofiño-Fabrés, Marijke Baltissen, Verena Schwach, Robert Passier, Gert Jan C Veenstra

引用次数: 0

Autism- and intellectual disability-associated MYT1L mutation alters human cortical interneuron differentiation, maturation, and physiology.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-15 DOI: 10.1016/j.stemcr.2025.102421

Ramachandran Prakasam, Julianna Determan, Gareth Chapman, Mishka Narasimhan, Renata Shen, Maamoon Saleh, Komal Kaushik, Paul Gontarz, Kesavan Meganathan, Bilal Hakim, Bo Zhang, James E Huettner, Kristen L Kroll

{"title":"Autism- and intellectual disability-associated MYT1L mutation alters human cortical interneuron differentiation, maturation, and physiology.","authors":"Ramachandran Prakasam, Julianna Determan, Gareth Chapman, Mishka Narasimhan, Renata Shen, Maamoon Saleh, Komal Kaushik, Paul Gontarz, Kesavan Meganathan, Bilal Hakim, Bo Zhang, James E Huettner, Kristen L Kroll","doi":"10.1016/j.stemcr.2025.102421","DOIUrl":"10.1016/j.stemcr.2025.102421","url":null,"abstract":"Myelin transcription factor 1 like (MYT1L) is a neuronal transcription factor highly expressed in the developing and adult brain, and, while pathogenic MYT1L mutations cause neurodevelopmental disorders, these have not been characterized in human models of neurodevelopment. Here, we modeled the consequences of pathogenic MYT1L mutation using human stem cell-derived cortical neurons, demonstrating that MYT1L mutation alters the differentiation trajectory, increasing neuronal gene expression, morphological complexity, and synapse production. We also examined consequences of MYT1L mutation in mature cortical interneurons, identifying hallmarks of impaired neuronal identity and maturation and correspondingly altered channel expression and electrophysiological properties. Finally, by defining MYT1L genome-wide occupancy in cortical interneurons, we identified direct MYT1L targets likely to mediate these phenotypes. Together, this work elucidates new MYT1L requirements for human cortical interneuron development and demonstrates how pathogenic MYT1L mutation perturbs this developmental program, contributing to the etiology of neurodevelopmental disorders.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102421"},"PeriodicalIF":5.9,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids. 化学定义和动态点击水凝胶支持人内耳类器官的毛细胞分化。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-11 Epub Date: 2025-01-09 DOI: 10.1016/j.stemcr.2024.12.001

Matthew R Arkenberg, Mahboubeh Jafarkhani, Chien-Chi Lin, Eri Hashino

{"title":"Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids.","authors":"Matthew R Arkenberg, Mahboubeh Jafarkhani, Chien-Chi Lin, Eri Hashino","doi":"10.1016/j.stemcr.2024.12.001","DOIUrl":"10.1016/j.stemcr.2024.12.001","url":null,"abstract":"The mechanical properties in the inner ear microenvironment play a key role in its patterning during embryonic development. To recapitulate inner ear development in vitro, three-dimensional tissue engineering strategies including the application of representative tissue models and scaffolds are of increasing interest. Human inner ear organoids are a promising model to recapitulate developmental processes; however, the current protocol requires Matrigel that contains ill-defined extracellular matrix components. Here, we implement an alternative, chemically defined, dynamic hydrogel to support the differentiation of human inner ear organoids. Specifically, thiol-norbornene and hydrazide-aldehyde click chemistries are used to fabricate inner ear organoid-laden, gelatin-based scaffolds. We identify optimal formulations to support hair cell development with comparable efficiency and fidelity to Matrigel-cultured organoids. These results suggest that the chemically defined hydrogel may serve as a viable alternative to Matrigel for inner ear tissue engineering.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102386"},"PeriodicalIF":5.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From responsibility to responsibilization in stem cell research: An ethical framework. 干细胞研究从责任到责任：伦理框架。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-11 Epub Date: 2025-01-09 DOI: 10.1016/j.stemcr.2024.102389

Lars S Assen, Annelien L Bredenoord, Rosario Isasi, Morris A Fabbri, Marianna A Tryfonidou, Karin R Jongsma

引用次数: 0

Transplantation of genome-edited retinal organoids restores some fundamental physiological functions coordinated with severely degenerated host retinas. 基因组编辑的视网膜类器官移植恢复了与严重退化的宿主视网膜协调的一些基本生理功能。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-11 Epub Date: 2025-01-16 DOI: 10.1016/j.stemcr.2024.102393

Mikiya Watanabe, Takayuki Yamada, Chieko Koike, Masayo Takahashi, Masao Tachibana, Michiko Mandai

{"title":"Transplantation of genome-edited retinal organoids restores some fundamental physiological functions coordinated with severely degenerated host retinas.","authors":"Mikiya Watanabe, Takayuki Yamada, Chieko Koike, Masayo Takahashi, Masao Tachibana, Michiko Mandai","doi":"10.1016/j.stemcr.2024.102393","DOIUrl":"10.1016/j.stemcr.2024.102393","url":null,"abstract":"We have previously shown that the transplantation of stem cell-derived retinal organoid (RO) sheets into animal models of end-stage retinal degeneration can lead to host-graft synaptic connectivity and restoration of vision, which was further improved using genome-edited Islet1-/- ROs (gROs) with a reduced number of ON-bipolar cells. However, the details of visual function restoration using this regenerative therapeutic approach have not yet been characterized. Here, we evaluated the electrophysiological properties of end-stage rd1 retinas after transplantation (TP-rd1) and compared them with those of wild-type (WT) retinas using multi-electrode arrays. Notably, retinal ganglion cells (RGCs) in TP-rd1 retinas acquired light sensitivity comparable to that of WT retinas. Furthermore, RGCs in TP-rd1 retinas showed light adaptation to a photopic background and responded to flickering stimuli. These results demonstrate that transplantation of gRO sheets may restore some fundamental physiological functions, possibly coordinating with the remaining functions in retinas with end-stage degeneration.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102393"},"PeriodicalIF":5.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective activation of FZD2 and FZD7 reveals non-redundant function during mesoderm differentiation. FZD2和FZD7的选择性激活揭示了在中胚层分化过程中的非冗余功能。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-11 Epub Date: 2025-01-16 DOI: 10.1016/j.stemcr.2024.102391

Rony Chidiac, Andy Yang, Elli Kubarakos, Nicholas Mikolajewicz, Hong Han, Maira P Almeida, Pierre E Thibeault, Sichun Lin, Graham MacLeod, Jean-Philippe Gratton, Jason Moffat, Stephane Angers

{"title":"Selective activation of FZD2 and FZD7 reveals non-redundant function during mesoderm differentiation.","authors":"Rony Chidiac, Andy Yang, Elli Kubarakos, Nicholas Mikolajewicz, Hong Han, Maira P Almeida, Pierre E Thibeault, Sichun Lin, Graham MacLeod, Jean-Philippe Gratton, Jason Moffat, Stephane Angers","doi":"10.1016/j.stemcr.2024.102391","DOIUrl":"10.1016/j.stemcr.2024.102391","url":null,"abstract":"During gastrulation, Wnt-β-catenin signaling dictates lineage bifurcation generating different mesoderm cell types. However, the specific role of Wnt receptors in mesoderm specification remains elusive. Using selective Frizzled (FZD) and LRP5/6 antibody-based agonists, we examined FZD receptors' function during directed mesoderm differentiation of human pluripotent stem cells (hPSCs). We found that FZD2 and FZD7 receptors are expressed at the membrane of hPSCs and that their activation triggers β-catenin signaling with different kinetics, thereby influencing mesoderm patterning choices. Specifically, FZD7 activation enhances both paraxial and lateral mesoderm differentiation, whereas FZD2 activation favors paraxial mesoderm. Mechanistically, FZD2 activation promotes sustained Wnt-β-catenin levels, guiding hPSCs differentiation toward paraxial mesoderm, while blocking lateral mesoderm. In contrast, FZD7 activation kinetics display similar initial activation but more dampening of β-catenin signaling, permitting lateral mesoderm induction in addition to paraxial mesoderm specification. Our findings reveal non-redundant roles for FZD2 and FZD7 in mesoderm specification, offering leverage for precise directed differentiation outcomes.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102391"},"PeriodicalIF":5.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient, non-viral arrayed CRISPR screening platform for iPSC-derived myeloid and microglia models.

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-02-11 DOI: 10.1016/j.stemcr.2025.102420

Sonja Meier, Anne Sofie Gry Larsen, Florian Wanke, Nicolas Mercado, Arianna Mei, Livia Takacs, Eva Suszanna Mracsko, Ludovic Collin, Martin Kampmann, Filip Roudnicky, Ravi Jagasia

引用次数: 0