Stem Cell Reports最新文献_第10页

Large-scale analysis of loss of chromosome Y in human pluripotent stem cells: Implications for Turner syndrome and ribosomopathies. 人类多能干细胞Y染色体缺失的大规模分析：对特纳综合征和核糖体病的影响。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-03 DOI: 10.1016/j.stemcr.2025.102471

Roni Sarel-Gallily, Keith M Gunapala, Nissim Benvenisty

引用次数: 0

Identifying enabling strategies for effective public dialogue in human embryo research. 确定在人类胚胎研究中进行有效公众对话的有利策略。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-05-01 DOI: 10.1016/j.stemcr.2025.102498

Matilda Beckett, Sarah Franklin, Peter J Rugg-Gunn

引用次数: 0

Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells. 参与成纤维细胞重编程为造血祖细胞的竞争动态基因调控网络。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-03 DOI: 10.1016/j.stemcr.2025.102473

Samiyah Shafiq, Kiyofumi Hamashima, Laura A Guest, Ali H Al-Anbaki, Fabio M R Amaral, Daniel H Wiseman, Valerie Kouskoff, Georges Lacaud, Yuin-Han Loh, Kiran Batta

{"title":"Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells.","authors":"Samiyah Shafiq, Kiyofumi Hamashima, Laura A Guest, Ali H Al-Anbaki, Fabio M R Amaral, Daniel H Wiseman, Valerie Kouskoff, Georges Lacaud, Yuin-Han Loh, Kiran Batta","doi":"10.1016/j.stemcr.2025.102473","DOIUrl":"10.1016/j.stemcr.2025.102473","url":null,"abstract":"Direct reprogramming of somatic cells offers a potentially safer therapeutic approach to generate patient-specific hematopoietic cells. However, this strategy is limited by stochasticity of reprogramming. Investigating the gene regulatory networks involved during reprogramming would help generate functional cells in adequate numbers. To address this, we developed an inducible system to reprogram fibroblasts to hematopoietic progenitor cells by ectopically expressing the two transcription factors SCL and LMO2. Transcriptome and epigenome analysis at different stages of reprogramming revealed uniform silencing of fibroblast genes and upregulation of the hemogenic endothelial program. Integrated analysis suggested that the transcription factors FLI1, GATA1/2, and KLF14 are direct targets of SCL/LMO2, which subsequently induce the hematopoietic program. Single-cell RNA sequencing revealed conflicting and competing fate decisions at intermediate stages of reprogramming. Inhibiting signaling pathways associated with competing neuronal fate enhanced reprogramming efficiency. In conclusion, this study identifies early/intermediate reprogramming events and associated pathways that could be targeted to improve reprogramming efficiency.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102473"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise promotes the functional integration of human stem cell-derived neural grafts in a rodent model of Parkinson's disease. 在帕金森病啮齿动物模型中，运动促进人类干细胞来源的神经移植物的功能整合。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-24 DOI: 10.1016/j.stemcr.2025.102480

Niamh Moriarty, Tyra D Fraser, Cameron P J Hunt, Georgia Eleftheriou, Jessica A Kauhausen, Lachlan H Thompson, Clare L Parish

{"title":"Exercise promotes the functional integration of human stem cell-derived neural grafts in a rodent model of Parkinson's disease.","authors":"Niamh Moriarty, Tyra D Fraser, Cameron P J Hunt, Georgia Eleftheriou, Jessica A Kauhausen, Lachlan H Thompson, Clare L Parish","doi":"10.1016/j.stemcr.2025.102480","DOIUrl":"10.1016/j.stemcr.2025.102480","url":null,"abstract":"Human pluripotent stem cell (hPSC)-derived dopamine neurons can functionally integrate and reverse motor symptoms in Parkinson's disease models, motivating current clinical trials. However, dopamine neuron proportions remain low and their plasticity inferior to fetal tissue grafts. Evidence shows exercise can enhance neuron survival and plasticity, warranting investigation for hPSC-derived neural grafts. We show voluntary exercise (wheel running) significantly increases graft plasticity, accelerating motor recovery in animals receiving ectopic, but not homotopic, placed grafts, suggestive of threshold requirements. Plasticity was accompanied by increased phosphorylated extracellular signal-regulated kinase (ERK+) cells in the graft (and host), reflective of mitogen-activated protein kinase (MAPK)-ERK signaling, a downstream target of glial cell-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), proteins that were also elevated. Verifying improved graft integration was the increase in cFos+ postsynaptic striatal neurons. These findings have direct implications for the adoption of physical therapy-based approaches to enhance neural transplantation outcomes in future Parkinson's disease clinical trials.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102480"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells. MIXL1在人诱导多能干细胞内胚层分化中的激活。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-24 DOI: 10.1016/j.stemcr.2025.102482

Pierre Osteil, Sarah Withey, Nicole Santucci, Nader Aryamanesh, Ignatius Pang, Nazmus Salehin, Jane Sun, Annie Qin, Jiayi Su, Hilary Knowles, Xiucheng Bella Li, Simon Cai, Ernst Wolvetang, Patrick P L Tam

引用次数: 0

Language and labels from the lab: Definitions in the stem cell-based embryo model debate. 来自实验室的语言和标签：基于干细胞的胚胎模型辩论中的定义。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102477

Hafez Ismaili M'hamdi

引用次数: 0

CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia. CD37在急性髓性白血病中通过整合素介导的信号传导调节白血病干细胞的自我更新。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102476

Jinyuan Lu, Lixin Lv, Xiaoxue Tian, Zheng Li, Yuting Ma, Nannan Li, Jian Wang, Guangming Wang, Yu Zeng, Wenjun Zhang, Jun Xu, Aibin Liang

{"title":"CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia.","authors":"Jinyuan Lu, Lixin Lv, Xiaoxue Tian, Zheng Li, Yuting Ma, Nannan Li, Jian Wang, Guangming Wang, Yu Zeng, Wenjun Zhang, Jun Xu, Aibin Liang","doi":"10.1016/j.stemcr.2025.102476","DOIUrl":"10.1016/j.stemcr.2025.102476","url":null,"abstract":"Leukemic stem cells (LSCs) are a small subset of leukemia cells that drive leukemia initiation and maintenance. Herein, we report that CD37, a member of transmembrane 4 superfamily (TM4SF), regulates the survival of acute myeloid leukemia (AML) cells as well as the self-renewal of AML LSCs. The downregulation of CD37 retarded proliferation and increased apoptosis in human AML cell lines THP-1 and OCI-AML2. Deficiency of CD37 in vivo had a minimal effect on normal hematopoiesis but significantly impeded leukemia maintenance and propagation, which led to increased apoptosis and decreased cell cycle entry in AML blasts as well as impaired colony formation and declined frequency of AML LSCs in the serial transplantation. Furthermore, CD37 interacted with integrin α4β7 and activated the phosphatidylinositol 3-kinase (PI3K)-AKT pathway mediated by integrin signaling. Our study provides novel insights for targeted therapy of AML, indicating CD37 as a safe and effective target for immunotherapy.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102476"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perinuclear mitochondrial clustering for mesenchymal-to-epithelial transition in pluripotency induction. 多能诱导中间质向上皮转化的核周线粒体聚类。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102474

Ge Xiang, Zihuang Liu, Zebin Yuan, Zhongfu Ying, Yingzhe Ding, Dongtong Lin, Haihao Qin, Shanshan Dong, Shihe Zhou, Hao Yuan, Wei Xie, Zhihong Zheng, Yongqiang Chen, Linpeng Li, Qi Long, Liang Yang, Yi Wu, Keshi Chen, Feixiang Bao, Yile Huang, Wei Li, Junwei Wang, Yang Liu, Dajiang Qin, Xingguo Liu

{"title":"Perinuclear mitochondrial clustering for mesenchymal-to-epithelial transition in pluripotency induction.","authors":"Ge Xiang, Zihuang Liu, Zebin Yuan, Zhongfu Ying, Yingzhe Ding, Dongtong Lin, Haihao Qin, Shanshan Dong, Shihe Zhou, Hao Yuan, Wei Xie, Zhihong Zheng, Yongqiang Chen, Linpeng Li, Qi Long, Liang Yang, Yi Wu, Keshi Chen, Feixiang Bao, Yile Huang, Wei Li, Junwei Wang, Yang Liu, Dajiang Qin, Xingguo Liu","doi":"10.1016/j.stemcr.2025.102474","DOIUrl":"10.1016/j.stemcr.2025.102474","url":null,"abstract":"Remodeled mitochondria are characteristic of pluripotent stem cells. However, a role for mitochondrial movement and distribution in pluripotency remains unknown. Here, we show that mitochondrial retrograde transport-mediated perinuclear clustering via dynein complex occurs at the early phase of pluripotency induction. Interestingly, this mitochondrial redistribution is regulated by Yamanaka factor OCT4 but not SOX2 or KLF4. This mitochondrial redistribution, which has effect on the efficiency of somatic cell reprogramming, also depends on DRP1-mediated mitochondrial fission. Importantly, perinuclear mitochondrial clustering is required for mesenchymal-to-epithelial transition (MET), an early step in reprogramming, during which β-catenin regulates the MET process. Furthermore, sufficient amount of β-catenin plays a key role in maintaining stabilization of E-CADHERIN. Taken together, these studies show that perinuclear mitochondrial clustering is an essential organellar step for MET process of pluripotency induction, which may shed light on the subcellular relationship between mitochondrial dynamics, pluripotency, and cellular morphology.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102474"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation. 在女性hiPSCs中，X染色体失活后的基因再激活是可预测的，但也是可变的，并通过分化持续存在。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-03 DOI: 10.1016/j.stemcr.2025.102472

Ana Cláudia Raposo, Paulo Caldas, Joana Jeremias, Maria Arez, Francisca Cazaux Mateus, Pedro Barbosa, Rui Sousa-Luís, Frederico Água, David Oxley, Annalisa Mupo, Melanie Eckersley-Maslin, Miguel Casanova, Ana Rita Grosso, Simão Teixeira da Rocha

{"title":"Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation.","authors":"Ana Cláudia Raposo, Paulo Caldas, Joana Jeremias, Maria Arez, Francisca Cazaux Mateus, Pedro Barbosa, Rui Sousa-Luís, Frederico Água, David Oxley, Annalisa Mupo, Melanie Eckersley-Maslin, Miguel Casanova, Ana Rita Grosso, Simão Teixeira da Rocha","doi":"10.1016/j.stemcr.2025.102472","DOIUrl":"10.1016/j.stemcr.2025.102472","url":null,"abstract":"Female human induced pluripotent stem cells frequently undergo X-chromosome inactivation (XCI) erosion, marked by X-inactive specific transcript (XIST) RNA loss and partial reactivation of the inactive X (Xi). This overlooked phenomenon limits our understanding of its impact on stem cell applications. Here, we show that XCI erosion is frequent and heterogeneous, leading to the reactivation of several X-linked genes. These are primarily located on the short arm of the X chromosome, particularly near escape genes and within H3K27me3-enriched domains, with reactivation linked to reduced promoter DNA methylation. Interestingly, escape genes further increase their expression from Xi upon XCI erosion, highlighting the critical role of XIST in their dosage regulation. Importantly, global (hydroxy)methylation levels and imprinted regions remain unaffected, and analysis of trilineage commitment and cardiomyocyte formation reveals that XCI erosion persists across differentiation. These findings underscore the need for greater awareness of the implications of XCI erosion for stem cell research and clinical applications.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102472"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safeguarding genomic imprints in naive human pluripotency. 保护人类多能性的基因组印记。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102475

Alejandro De Los Angeles

引用次数: 0