Stem Cell Reports最新文献_第10页

Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. 常驻间充质祖细胞在稳态和再生骨骼肌中需要自分泌igf - 1。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-25 DOI: 10.1016/j.stemcr.2026.102973

Yangyi E Luo, Zoe Abe-Teh, Tarek Y Alsaghir, Li-Ying Kuo, Fahong Yu, Bradley E Stoker, Yumei Zhou, Ambili Bai Appu, Elijah M Dy, Feng Yue, Daniel Kopinke, Elisabeth R Barton

{"title":"Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle.","authors":"Yangyi E Luo, Zoe Abe-Teh, Tarek Y Alsaghir, Li-Ying Kuo, Fahong Yu, Bradley E Stoker, Yumei Zhou, Ambili Bai Appu, Elijah M Dy, Feng Yue, Daniel Kopinke, Elisabeth R Barton","doi":"10.1016/j.stemcr.2026.102973","DOIUrl":"10.1016/j.stemcr.2026.102973","url":null,"abstract":"Fibro-adipogenic progenitors (FAPs) are muscle-resident mesenchymal stromal cells essential for homeostasis and regeneration but can produce fibrosis or intramuscular fat in pathological conditions. Insulin-like growth factor-I (IGF-I) regulates regeneration through actions on muscle fibers, satellite cells, macrophages, and extracellular matrix (ECM) remodeling but has multiple sources. To assess the role of FAP-derived IGF-I, we generated inducible FAP-specific Igf1-deficient (FID) mice. Following BaCl2 injury, FID muscles displayed impaired regeneration, with smaller fibers, fewer Pax7+ and MyoD+ cells, increased CD68+ macrophages, decreased collagen, and suppressed FAP proliferation. After glycerol-induced injury, FID muscles had reduced fat. Primary FID FAPs displayed blunted proliferation, upregulated immune-regulatory genes, and downregulated ECM and growth genes, with delayed fibrogenic and adipogenic differentiation. scRNA-seq of homeostatic muscle revealed reduced protein translation and ECM indices alongside increased senescence markers in FID samples. Taken together, FAP IGF-I is critical for FAP function, with direct and indirect impact on muscle regeneration.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102973"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148333303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early replication fragile sites are associated with cancer-related CNVs and SNVs in human embryonic stem cells. 人类胚胎干细胞的早期复制脆弱位点与癌症相关的cnv和snv相关。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-18 DOI: 10.1016/j.stemcr.2026.102968

Yu-Ping Dong, Menglin Qiu, Haoyu Tang, Wen Shi, Yi Lu, Fang Ji, Hongwei Liao, Songmin Ying, Ping Zheng, Lin Wang

{"title":"Early replication fragile sites are associated with cancer-related CNVs and SNVs in human embryonic stem cells.","authors":"Yu-Ping Dong, Menglin Qiu, Haoyu Tang, Wen Shi, Yi Lu, Fang Ji, Hongwei Liao, Songmin Ying, Ping Zheng, Lin Wang","doi":"10.1016/j.stemcr.2026.102968","DOIUrl":"10.1016/j.stemcr.2026.102968","url":null,"abstract":"Long-term culture of human embryonic stem cells (hESCs) often induces chromosomal abnormalities, which limits their clinical use. However, the underlying mechanisms are unclear. Early replication fragile sites (ERFSs) are genomic loci susceptible to breakage in early S-phase and serve as hotspots for chromosomal rearrangements, with established links to carcinogenesis. To map ERFSs in hESCs, we established the early S-phase synchronization protocols and identified ERFSs. These ERFSs are enriched in GC content and short interspersed nuclear elements (SINEs) and are frequently located in promoters or enhancers of genes involved in pluripotency, proliferation, and genomic stability. ERFSs also overlap with regions associated with copy number variants (CNVs) and single nucleotide variants (SNVs) linked to cancers. Furthermore, we found that chromatin accessibility contributes to ERFS formation. Collectively, these findings provide a key resource for advancing ERFS research, offering insights into the phenotypic and genomic alterations observed in long-term hESC cultures.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102968"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Closing the gap? China's new regulation on biomedical technologies. 缩小差距？中国对生物医学技术的新规定。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-25 DOI: 10.1016/j.stemcr.2026.102978

Ubaka Ogbogu

引用次数: 0

aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo signaling kinase LATS1. aPKC-ζ III通过改变Par-3与Hippo信号激酶LATS1的相互作用促进滋养细胞融合。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-25 DOI: 10.1016/j.stemcr.2026.102975

Sumaiyah Z Shaha, Wendy K Duan, Juan Garcia Rivas, Ivan K Domingo, Meghan Riddell

{"title":"aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo signaling kinase LATS1.","authors":"Sumaiyah Z Shaha, Wendy K Duan, Juan Garcia Rivas, Ivan K Domingo, Meghan Riddell","doi":"10.1016/j.stemcr.2026.102975","DOIUrl":"10.1016/j.stemcr.2026.102975","url":null,"abstract":"The first trimester of pregnancy is a critical developmental period for the placenta. In humans, the maternal-facing exchange surface is formed by a single giant multinucleate syncytium: the syncytiotrophoblast (ST). The ST arises from villous lineage commitment of trophoblast stem cells (TSC) and the differentiation and fusion of progenitor cytotrophoblasts (pCT) to form the multinucleate syncytium. The Hippo signaling co-transcription factor YAP1 promotes pCT maintenance and TSC stemness; however, how Hippo signaling is regulated remains unknown. We have identified a novel PRKCZ-encoded aPKC isoform, aPKC-ζ III, that is highly expressed in pCT and ST. Here, we establish that aPKC-ζ III promotes pCT fusion by activation of Hippo signaling. Specifically, aPKC-ζ III outcompetes the Hippo kinase LATS1 for scaffolding protein Par-3 binding, resulting in YAP1 inactivation and pCT fusion. Our findings identify a key modulator of Hippo signaling in human trophoblasts that is critical for first-trimester ST differentiation.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102975"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148333279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of the naive pluripotency gene network with the response to GSK3 inhibition by Tcf7l1-driven enhancer decommissioning. tcf7l1驱动的增强子退役将幼稚多能基因网络与GSK3抑制应答整合起来。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-07-02 DOI: 10.1016/j.stemcr.2026.102980

Jieun Kwon, Jenny Y Zhang, Brian R Shy, Matthew S MacDougall, Eva H W Leung, Constantinos Chronis, Fotini Gounari, Bradley J Merrill

{"title":"Integration of the naive pluripotency gene network with the response to GSK3 inhibition by Tcf7l1-driven enhancer decommissioning.","authors":"Jieun Kwon, Jenny Y Zhang, Brian R Shy, Matthew S MacDougall, Eva H W Leung, Constantinos Chronis, Fotini Gounari, Bradley J Merrill","doi":"10.1016/j.stemcr.2026.102980","DOIUrl":"10.1016/j.stemcr.2026.102980","url":null,"abstract":"The transition out of naive pluripotency requires the simultaneous dissolution of the naive gene regulatory network as well as a redirection of transcriptional response to WNT/β-CATENIN signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at TCF7L1-bound cis-regulatory elements in mouse pluripotent stem cells. A combination of gene knockout and inducible expression lines of ESCs was generated to experimentally isolate effects of Tcf/Lef factors on pluripotency. Naive cis-regulatory elements were specifically bound by and rapidly decommissioned by TCF7L1 upon releasing cells from GSK3 inhibition. These findings suggest that Tcf7l1-mediated enhancer decommissioning serves as a critical mechanism by which pluripotent cells rapidly alter the response to signaling pathways in preparation for gastrulation, and it may be a mechanism used in diverse cell types for modulating the effects of WNT/β-CATENIN signaling pathway in a cell-specific manner to stimulate a variety of cell fate decisions.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102980"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148377029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b. 通过过表达Dnmt3b减少雄激素单倍体胚胎干细胞产生的半克隆小鼠的自我二倍体化和提高存活率。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-03 DOI: 10.1016/j.stemcr.2026.102974

Wenteng He, Xiaobai Zhang, Yalin Zhang, Weisheng Zheng, Zeyu Xiong, Xinjie Hu, Mingzhu Wang, Linfeng Zhang, Kun Zhao, Zhibin Qiao, Weiyi Lai, Cong Lv, Xiaochen Kou, Yanhong Zhao, Jiqing Yin, Wenqiang Liu, Yonghua Jiang, Mo Chen, Ruimin Xu, Rongrong Le, Chong Li, Hong Wang, Xiaoping Wan, Hailin Wang, Zhiming Han, Cizhong Jiang, Shaorong Gao, Jiayu Chen

引用次数: 0

Single-cell multiomic approaches define a gradual, spatially regulated epigenetic and transcriptional transition from embryonic to adult neural stem cells. 单细胞多组学方法定义了从胚胎到成体神经干细胞的一个渐进的、空间调控的表观遗传和转录转变。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-18 DOI: 10.1016/j.stemcr.2026.102967

Beatrix S Wang, Konstantina Karamboulas, Nareh Tahmasian, Daniel J Dennis, David R Kaplan, Freda D Miller

{"title":"Single-cell multiomic approaches define a gradual, spatially regulated epigenetic and transcriptional transition from embryonic to adult neural stem cells.","authors":"Beatrix S Wang, Konstantina Karamboulas, Nareh Tahmasian, Daniel J Dennis, David R Kaplan, Freda D Miller","doi":"10.1016/j.stemcr.2026.102967","DOIUrl":"10.1016/j.stemcr.2026.102967","url":null,"abstract":"Here, we ask how adult neural stem cells (NSCs) arise developmentally, focusing on murine cortical precursors that generate excitatory neurons embryonically and interneurons and glial cells postnatally. Using complementary single-cell spatial, transcriptomic, and epigenomic approaches, we show that postnatal NSC state acquisition involves a gradual transcriptional and epigenetic shift in the entire embryonic cortical precursor cell population and identify a distinct transition precursor state at E17/18 when both embryonic and the first postnatal progeny are being generated. Non-proliferative adult NSCs are also first seen at this transition timepoint, but they arise in a spatial domain distinct from that of the first postnatal progeny, indicating that NSC state acquisition is not a necessary prelude to the switch in cell genesis. These findings support a gradual epigenetically continuous model for the transition from developing cortical precursors to NSCs and show that this is spatially separable from the transition to generating postnatal cell types.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102967"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical vascular organoid therapy promotes microvascular regeneration and functional recovery in porcine ischemic cardiomyopathy. 局部血管类器官治疗促进猪缺血性心肌病微血管再生和功能恢复。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-07-09 DOI: 10.1016/j.stemcr.2026.102999

John A Farag, Shin Yajima, Yujiro Kawai, Koji Kawago, Eric Pfrender, Umayr Syed, Jennifer K Lyons, Tsuyoshi Ueyama, Hiroyuki Takashima, Yuka Matsuura, Gentaro Ikeda, Yu Liu, Yuanjia Zhu, Stefan Elde, Phillip C Yang, Y Joseph Woo, Yasuhiro Shudo

{"title":"Topical vascular organoid therapy promotes microvascular regeneration and functional recovery in porcine ischemic cardiomyopathy.","authors":"John A Farag, Shin Yajima, Yujiro Kawai, Koji Kawago, Eric Pfrender, Umayr Syed, Jennifer K Lyons, Tsuyoshi Ueyama, Hiroyuki Takashima, Yuka Matsuura, Gentaro Ikeda, Yu Liu, Yuanjia Zhu, Stefan Elde, Phillip C Yang, Y Joseph Woo, Yasuhiro Shudo","doi":"10.1016/j.stemcr.2026.102999","DOIUrl":"10.1016/j.stemcr.2026.102999","url":null,"abstract":"Ischemic heart disease (IHD) often progresses to heart failure due to persistent microvascular dysfunction not corrected by conventional epicardial revascularization. We developed a scaffold-free vascular organoid composed of human endothelial progenitor cells (EPCs) and mesenchymal stem cell-derived smooth muscle cells (SMCs) and evaluated its therapeutic potential in a porcine IHD model. Fourteen days after ischemia induction, bilayer EPC-SMC organoids were transplanted onto the left ventricular surface, and animals were followed for four weeks. Cardiac MRI demonstrated the preservation of left ventricular ejection fraction and modest improvement in regional function compared with controls. Histological and immunohistochemical analyses revealed the migration of transplanted cells into host myocardium, increased vascular density, and enhanced vessel maturation, while gene expression profiling showed the upregulation of angiogenesis-related genes. These findings demonstrate that scaffold-free EPC-SMC vascular organoids can engraft, promote microvascular remodeling, and preserve cardiac function after ischemic injury.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102999"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148424354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional maturation of enteric neurons derived from human induced pluripotent stem cells. 人诱导多能干细胞肠神经元的功能成熟。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-04 DOI: 10.1016/j.stemcr.2026.102942

Eve S Rowland, Maciej Daniszewski, Caio Seguin, Maria A Di Biase, Gunes S Yildiz, Atefeh Namipashaki, Alice Pébay, Faranak Fattahi, Lincon A Stamp, Marlene M Hao

{"title":"Functional maturation of enteric neurons derived from human induced pluripotent stem cells.","authors":"Eve S Rowland, Maciej Daniszewski, Caio Seguin, Maria A Di Biase, Gunes S Yildiz, Atefeh Namipashaki, Alice Pébay, Faranak Fattahi, Lincon A Stamp, Marlene M Hao","doi":"10.1016/j.stemcr.2026.102942","DOIUrl":"10.1016/j.stemcr.2026.102942","url":null,"abstract":"The enteric nervous system (ENS) plays a crucial role in regulating gastrointestinal function, including motility, secretion, and absorption. Enteric neurons and glia derived from human induced pluripotent stem cells (iPSCs) have vast applications in disease modeling, drug screening, and cell replacement therapy, yet our understanding of their functional maturation remains limited. Using a defined protocol for the generation of iPSC-derived enteric neurons, we examined neuronal differentiation with immunohistochemistry, gene expression analysis, and cellular-resolution calcium (Ca2+) imaging from day (D)21 to D57 of differentiation. By D21, cells expressing pan-neuronal markers were present, and at D22 the cultures were responsive to K+-induced depolarization as well as neurotransmitter receptor agonists, with maturation of responses to high K+ depolarization during the differentiation period. In addition, the development of spontaneous neuronal network activity was observed at 2 months post-differentiation. Together these findings provide a timeline of the functional maturation of iPSC-derived ENS cells.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102942"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148164334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of voluntary exercise, diet, and selenium on hypothalamic adult neurogenesis. 自主运动、饮食和硒对下丘脑成人神经发生的影响。

IF 5.6 2区医学

Stem Cell Reports Pub Date : 2026-07-14 Epub Date: 2026-06-11 DOI: 10.1016/j.stemcr.2026.102953

Sara K M Jörgensen, Rachel E Martin, Andrew Want, James Morgan, David Petrik

{"title":"Effects of voluntary exercise, diet, and selenium on hypothalamic adult neurogenesis.","authors":"Sara K M Jörgensen, Rachel E Martin, Andrew Want, James Morgan, David Petrik","doi":"10.1016/j.stemcr.2026.102953","DOIUrl":"10.1016/j.stemcr.2026.102953","url":null,"abstract":"Hypothalamic adult neurogenesis is implicated in energy homeostasis; however, the impact of physical exercise, particularly under high-fat diet (HFD) conditions, on this process remains unclear. To address this, we subjected mice to short-term and long-term voluntary running under control and HFD conditions. We show that long-term, but not short-term, running upregulates hypothalamic neurogenesis in Control mice. Conversely, short-term running rescues HFD-induced neurogenesis in the median eminence (ME), promoting the differentiation and survival of newly generated neurons. This rescue effect is absent with long-term running. Furthermore, we show that selenium mimics the effects of short-term running on ME neurogenesis and increases the activation and proliferation of hypothalamic adult neural stem cells, suggesting its role as a hypothalamic exerkine. Our findings indicate that voluntary exercise differentially influences adult neurogenesis in the hypothalamus compared to the hippocampus, with its neurogenic effects being modulated by diet, exercise duration, and regional differences within hypothalamic compartments.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102953"},"PeriodicalIF":5.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0