Kouta Niizuma, Satoru Morikawa, Eric Gars, Jinyi Xiang, Tomoko Matsubara-Takahashi, Rei Saito, Eri Takematsu, Yuting Wang, Haojun Xu, Arata Wakimoto, Tze Kai Tan, Yoshiaki Kubota, Charles K F Chan, Irving L Weissman, Taneaki Nakagawa, Adam C Wilkinson, Hiromitsu Nakauchi, Ryo Yamamoto
{"title":"Elevated hematopoietic stem cell frequency in mouse alveolar bone marrow.","authors":"Kouta Niizuma, Satoru Morikawa, Eric Gars, Jinyi Xiang, Tomoko Matsubara-Takahashi, Rei Saito, Eri Takematsu, Yuting Wang, Haojun Xu, Arata Wakimoto, Tze Kai Tan, Yoshiaki Kubota, Charles K F Chan, Irving L Weissman, Taneaki Nakagawa, Adam C Wilkinson, Hiromitsu Nakauchi, Ryo Yamamoto","doi":"10.1016/j.stemcr.2024.11.004","DOIUrl":null,"url":null,"abstract":"<p><p>Hematopoietic stem cells (HSCs) are crucial for maintaining hematopoietic homeostasis and are localized within distinct bone marrow (BM) niches. While BM niches are often considered similar across different skeletal sites, we discovered that the alveolar BM (al-BM) in the mandible harbors the highest frequency of immunophenotypic HSCs in nine different skeletal sites. Transplantation assays revealed significantly increased engraftment from al-BM compared to femur, tibia, or pelvis BM, likely due to a higher proportion of alveolar HSCs. Moreover, hematopoietic progenitor cells (c-Kit<sup>+</sup> Sca-1<sup>+</sup> Lin<sup>-</sup>) in al-BM exhibited increased quiescence and reduced apoptosis, indicating superior maintenance and survival characteristics. We also observed an enrichment of mesenchymal stromal cells and skeletal stem cells in al-BM, suggesting a more supportive microenvironment. These findings indicate that al-BM provides a unique microenvironment conducive to higher frequency of HSCs, offering new insights into site-specific hematopoiesis.</p>","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102374"},"PeriodicalIF":5.9000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem Cell Reports","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.stemcr.2024.11.004","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}
Elevated hematopoietic stem cell frequency in mouse alveolar bone marrow.
Hematopoietic stem cells (HSCs) are crucial for maintaining hematopoietic homeostasis and are localized within distinct bone marrow (BM) niches. While BM niches are often considered similar across different skeletal sites, we discovered that the alveolar BM (al-BM) in the mandible harbors the highest frequency of immunophenotypic HSCs in nine different skeletal sites. Transplantation assays revealed significantly increased engraftment from al-BM compared to femur, tibia, or pelvis BM, likely due to a higher proportion of alveolar HSCs. Moreover, hematopoietic progenitor cells (c-Kit+ Sca-1+ Lin-) in al-BM exhibited increased quiescence and reduced apoptosis, indicating superior maintenance and survival characteristics. We also observed an enrichment of mesenchymal stromal cells and skeletal stem cells in al-BM, suggesting a more supportive microenvironment. These findings indicate that al-BM provides a unique microenvironment conducive to higher frequency of HSCs, offering new insights into site-specific hematopoiesis.
期刊介绍:
Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.