Science China Life Sciences最新文献_第4页

Disruption of the miR396 binding site in GROWTH-REGULATING FACTOR 4 enhances grain size in wheat.

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-04 DOI: 10.1007/s11427-024-2878-9

Boshu Li, Xinwei Jiang, Zhuangzhuang Chai, Jinxing Liu, Caixia Gao, Kunling Chen

引用次数: 0

m⁶A reader YTHDF2 governs the onset of atrial fibrillation by modulating Cacna1c translation. m6A 读者 YTHDF2 通过调节 Cacna1c 的翻译来控制心房颤动的发生。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-10-18 DOI: 10.1007/s11427-024-2674-2

Chuansheng Chen, Guanghua Wang, Qicheng Zou, Ke Xiong, Zhiwen Chen, Beihua Shao, Yi Liu, Duanyang Xie, Yong Ji

{"title":"m6A reader YTHDF2 governs the onset of atrial fibrillation by modulating Cacna1c translation.","authors":"Chuansheng Chen, Guanghua Wang, Qicheng Zou, Ke Xiong, Zhiwen Chen, Beihua Shao, Yi Liu, Duanyang Xie, Yong Ji","doi":"10.1007/s11427-024-2674-2","DOIUrl":"10.1007/s11427-024-2674-2","url":null,"abstract":"Atrial fibrillation (AF) is the most common arrhythmia, which is tightly associated with the abnormal expression and function of ion channels in the atrial cardiomyocytes. N6-methyladenosine (m6A), a widespread chemical modification in eukaryotic mRNA, is known to play a significant regulatory role in the pathogenesis of heart disease. However, the significance of m6A regulatory proteins in the onset of AF remains unclear. Here, we demonstrate that the m6A reader protein YTHDF2 regulates atrial electrical remodeling and AF onset by modulating the Cav1.2 expression. Firstly, YTHDF2 expression was selectively upregulated in rat atrial cardiomyocytes with AF. Secondly, YTHDF2 knockout reduced AF susceptibility in mice. Thirdly, the knockout of YTHDF2 increased Cav1.2 protein levels in an m6A-in-dependent manner, ultimately prolonging the atrial myocardial refractory period, a critical electrophysiological substrate for the onset of AF. Fourthly, the N-terminal domain of YTHDF2 was identified as critical for Cacna1c mRNA translation regulation. Overall, our findings unveil that YTHDF2 can alter Cav1.2 protein expression in an m6A-independent manner, thereby facilitating the onset of AF. Our study suggests that YTHDF2 may be a potential intervention target for AF.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"706-721"},"PeriodicalIF":8.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative genome editing in plants: a transposase and CRISPR combination approach. 创新的植物基因组编辑：转座酶和 CRISPR 组合方法。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-11-01 DOI: 10.1007/s11427-024-2729-2

Hamza Sohail, Iqra Noor, Xuehao Chen, Xiaodong Yang

引用次数: 0

Establishing age-group specific reference intervals of human salivary proteome and its preliminary application for epilepsy diagnosis. 建立人唾液蛋白质组的年龄组特异性参考区间及其在癫痫诊断中的初步应用。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-12-25 DOI: 10.1007/s11427-024-2730-6

Nianci Xue, Xia Xia, Yini Wang, Xianju Li, Nairen Zheng, Yi Wang, Baoying Gong, Bin Zhang, Yanjia Chen, Yue Chen, Yanjuan Li, Hong Cao, Wofeng Liu, Hongqiang Huang, Shuo Yang, Lisen Sui, Lin Meng, Jianwen Guo, Jun Qin

{"title":"Establishing age-group specific reference intervals of human salivary proteome and its preliminary application for epilepsy diagnosis.","authors":"Nianci Xue, Xia Xia, Yini Wang, Xianju Li, Nairen Zheng, Yi Wang, Baoying Gong, Bin Zhang, Yanjia Chen, Yue Chen, Yanjuan Li, Hong Cao, Wofeng Liu, Hongqiang Huang, Shuo Yang, Lisen Sui, Lin Meng, Jianwen Guo, Jun Qin","doi":"10.1007/s11427-024-2730-6","DOIUrl":"10.1007/s11427-024-2730-6","url":null,"abstract":"Salivary proteins serve multifaceted roles in maintaining oral health and hold significant potential for diagnosing and monitoring diseases due to the non-invasive nature of saliva sampling. However, the clinical utility of current saliva biomarker studies is limited by the lack of reference intervals (RIs) to correctly interpret the testing result. Here, we developed a rapid and robust saliva proteome profiling workflow, obtaining coverage of >1,200 proteins from a 50-µL unstimulated salivary flow with 30 min gradients. With the workflow, we investigated protein variation in a cohort of 1,743 healthy individuals. The significant differences in non-linear saliva proteomes among age groups resulted in the establishment of age-related RIs covering 1,123 salivary protein variations. We then utilized a cohort of 30 epilepsy patients as a case study to illustrate the practical application of RIs in identifying disease-enriched outlier proteins, elucidating their cellular origins, determining disease diagnosis, and visualizing outlier proteins in each epilepsy patient. Our study showed the classification model based on the RI achieved PR-AUC of 0.815 (95%CI: 0.813-0.826). Additionally, we validated these results in an independent test set. Furthermore, the epilepsy cohort could be further stratified into 2 major subtypes, with one subtype characterized by disrupted metabolic proteins and containing mostly Focal Cortical Dysplasia (FCD) type III epilepsy patients. Overall, our study provided a proof-of-principle workflow for the use of salivary proteome for health monitoring, epilepsy diagnosis and subtyping.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"809-824"},"PeriodicalIF":8.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FHL2 deficiency aggravates Candida albicans infection through decreased myelopoiesis. FHL2缺乏通过减少骨髓生成加重白色念珠菌感染。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-08 DOI: 10.1007/s11427-024-2645-y

Rongmei Gao, Kanchao Chen, Yimin Wang, Rongxia Guo, Xiaoyu Zhang, Peng Wu, Weili Wang, Qingxiang Huang, Xuemei Xie, Shangda Yang, Yanling Lv, Qian Ren, Fei Liu, Song Chen, Fengxia Ma, Tao Cheng, Hui Cheng

引用次数: 0

Strategic codon selection for enhanced genetic code expansion. 增强遗传密码扩展的策略密码子选择。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-11-28 DOI: 10.1007/s11427-024-2758-2

Zhouqing Luo, Junbiao Dai

引用次数: 0

Green revolution gene drives adaptation of Arabidopsis to the extremely high altitude.

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.1007/s11427-024-2769-x

Xing-Hui Hou, Yong-Chao Xu, Tianshu Sun, Yan-Bo Gong, Xin-Tong Li, Guang-Teng Jin, Yu-Tao Bian, Yi-Ni Liu, Juan Jiang, Xiao-Min Niu, Hongya Gu, Ya-Long Guo

{"title":"Green revolution gene drives adaptation of Arabidopsis to the extremely high altitude.","authors":"Xing-Hui Hou, Yong-Chao Xu, Tianshu Sun, Yan-Bo Gong, Xin-Tong Li, Guang-Teng Jin, Yu-Tao Bian, Yi-Ni Liu, Juan Jiang, Xiao-Min Niu, Hongya Gu, Ya-Long Guo","doi":"10.1007/s11427-024-2769-x","DOIUrl":"10.1007/s11427-024-2769-x","url":null,"abstract":"To elucidate the process of adaptation, particularly the traits subject to natural selection and the molecular mechanisms underlying their natural variation, is one of the primary objectives of evolutionary biology. The uplifted landscape offers an excellent framework for understanding how organisms adapt to dramatic climatic gradients. To investigate the genetic basis of plant adaptation to the extremely high altitude, we first compared the genomic and phenotypic variations of two closely related Arabidopsis thaliana accessions from high altitude (Xizang, also known as \"Tibet\") and low altitude (Yunnan), respectively. The Xizang population represents a relict group characterized by a small effective population size. Notably, the Xizang genome has more transposable elements (TEs) and more gene loss-of-function (LoF) mutations. Differentially expressed genes were enriched in biological processes of cellular response to oxygen-containing compound, regulation of defense response, and response to light intensity. Intriguingly, the phenotypic selection analysis revealed that silique density was under natural selection. Furthermore, we genetically mapped and validated that the LoF mutation of GA20ox1, the homologous gene of green revolution in rice, resulted in a higher silique density in Xizang Arabidopsis. Given that GA20ox1 is linked to Arabidopsis adaptation to the Alps Mountains, its parallel evolution plays an important role in the adaptation to Alpine habitats. Overall, our results highlight that identifying adaptive traits and elucidating the molecular mechanisms underlying natural variation of these traits is crucial for unraveling the mystery of adaptive evolution and has significant implications for crop breeding.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"859-870"},"PeriodicalIF":8.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing CRISPR base editing technology through innovative strategies and ideas. 通过创新战略和理念推进 CRISPR 碱基编辑技术。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-09-02 DOI: 10.1007/s11427-024-2699-5

Xiongwei Fan, Yang Lei, Liren Wang, Xiushan Wu, Dali Li

{"title":"Advancing CRISPR base editing technology through innovative strategies and ideas.","authors":"Xiongwei Fan, Yang Lei, Liren Wang, Xiushan Wu, Dali Li","doi":"10.1007/s11427-024-2699-5","DOIUrl":"10.1007/s11427-024-2699-5","url":null,"abstract":"The innovation of CRISPR/Cas gene editing technology has developed rapidly in recent years. It is widely used in the fields of disease animal model construction, biological breeding, disease diagnosis and screening, gene therapy, cell localization, cell lineage tracking, synthetic biology, information storage, etc. However, developing idealized editors in various fields is still a goal for future development. This article focuses on the development and innovation of non-DSB editors BE and PE in the platform-based CRISPR system. It first explains the application of ideas for improvement such as \"substitution\", \"combination\", \"adaptation\", and \"adjustment\" in BE and PE development and then catalogues the ingenious inversions and leaps of thought reflected in the innovations made to CRISPR technology. It will then elaborate on the efforts currently being made to develop small editors to solve the problem of AAV overload and summarize the current application status of editors for in vivo gene modification using AAV as a delivery system. Finally, it summarizes the inspiration brought by CRISPR/Cas innovation and assesses future prospects for development of an idealized editor.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"610-627"},"PeriodicalIF":8.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant diversity increases diversity and network complexity rather than alters community assembly processes of leaf-associated fungi in a subtropical forest. 植物多样性增加了亚热带森林中叶相关真菌的多样性和网络复杂性，而不是改变了群落组装过程。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-10-18 DOI: 10.1007/s11427-024-2630-6

Jie Li, Xing-Chun Li, Hui-Yun Gan, Yue Zhang, Zi-Xuan Guo, Yu-Xuan Liu, Yong-Qing Lin, Liang-Dong Guo

{"title":"Plant diversity increases diversity and network complexity rather than alters community assembly processes of leaf-associated fungi in a subtropical forest.","authors":"Jie Li, Xing-Chun Li, Hui-Yun Gan, Yue Zhang, Zi-Xuan Guo, Yu-Xuan Liu, Yong-Qing Lin, Liang-Dong Guo","doi":"10.1007/s11427-024-2630-6","DOIUrl":"10.1007/s11427-024-2630-6","url":null,"abstract":"Plant diversity significantly impacts ecosystem processes and functions, yet its influence on the community assembly of leaf fungi remains poorly understood. In this study, we investigated leaf epiphytic and endophytic fungal communities in a Chinese subtropical tree species richness experiment, ranging from 1 to 16 species, using amplicon sequencing to target the internal transcribed spacer 1 region of the rDNA. We found that the community assembly of epiphytic and endophytic fungi was predominantly governed by stochastic processes, with a higher contribution of dispersal limitation on epiphytic than on endophytic fungal communities but a higher contribution of selection on endophytic than on epiphytic fungal communities. The plant-epiphytic fungus interaction network was more complex (e.g., more highly connected and strongly nested but less specialized and modularized) than the plant-endophytic fungus interaction network. Additionally, tree species richness was positively correlated with the network complexity and diversity of epiphytic (α-, β- and γ-diversity) and endophytic (β- and γ-diversity) fungi, but was not associated with the contribution of the stochastic and deterministic processes on the community assembly of epiphytic and endophytic fungi. This study highlights that tree species diversity enhances the diversity and network complexity, rather than alters the ecological processes in community assembly of leaf-associated fungi.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"846-858"},"PeriodicalIF":8.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoinhibition and cofactors of a helper NLR. 辅助NLR的自身抑制和辅助因子。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-03-01 Epub Date: 2024-12-04 DOI: 10.1007/s11427-024-2708-0

Hongyuan Zheng, Xuemin Zhou, Chenyu Zhao, Daowen Wang, Zhengqing Fu

引用次数: 0