Science China Life Sciences最新文献_第3页

The discovery of tiny RNAs that have crucial roles. 微小rna的发现起着至关重要的作用。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2024-12-28 DOI: 10.1007/s11427-024-2819-y

Shouhong Guang, Mo-Fang Liu

引用次数: 0

Reanalysis of whole genome sequencing ends a diagnostic Odyssey of neurodevelopmental disorders caused by RNU4-2 variants.

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2025-02-17 DOI: 10.1007/s11427-024-2829-2

Shiqi Fan, Shuanghao Yang, Miao Sun, Weiyue Gu, Xue Zhang

引用次数: 0

Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages. 代谢组学和RNA-seq揭示了不同年龄肥瘦猪骨骼肌代谢和分泌代谢谱的差异。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2025-01-10 DOI: 10.1007/s11427-024-2654-2

Liu Guo, Junfei Xu, Wenyue Zhou, Sisi Chen, Hanjing Shi, Mengmeng Han, Zekun Yang, Yehui Duan, Weijun Pang, Yulong Yin, Fengna Li

{"title":"Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages.","authors":"Liu Guo, Junfei Xu, Wenyue Zhou, Sisi Chen, Hanjing Shi, Mengmeng Han, Zekun Yang, Yehui Duan, Weijun Pang, Yulong Yin, Fengna Li","doi":"10.1007/s11427-024-2654-2","DOIUrl":"10.1007/s11427-024-2654-2","url":null,"abstract":"Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity, aging and metabolic disease. Since obese and lean pigs are ideal models for metabolic research. Here, we compared metabolome and transcriptome of Longissimus dorsi (LD) muscle between Taoyuan black (TB, obese) and Duroc (lean) pigs at different ages. We defined the \"window phase\" of intramuscular fat (IMF) deposition in TB pig, which has significantly higher IMF than Duroc pig. Our results displayed discrepant lipid composition and different expression genes (DEGs) enriched in lipid metabolism, and both metabolome and transcriptome analyses revealed stronger energy expenditure and more active amino acid and protein metabolism in Duroc pig. 10 up- and 51 down-regulated biomarker metabolites with age- and breed-specificity were identified. Potential secretory metabolites, including organic acid (fumaric acid, succinate, malic acid, and gamma-aminobutyric acid), amino acid (L-lysine, and L-glutamic acid), lipid (2-hydroxyisovaleric acid, and L-carnitine) were demonstrated a significant correlation with IMF deposition. Our research highlights the huge difference of metabolic spectrum in skeletal muscle between obese and lean model and muscle-derived secretory metabolites might act as an ambassador of intercellular communication to regulate systematic metabolism.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"1102-1117"},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peri-centrosomal localization of small interfering RNAs in C. elegans. 秀丽隐杆线虫小干扰rna在中心体周围的定位。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2025-01-14 DOI: 10.1007/s11427-024-2818-7

Qile Jin, Xuezhu Feng, Minjie Hong, Ke Wang, Xiangyang Chen, Jiewei Cheng, Yan Kuang, Xiaoyue Si, Mingjing Xu, Xinya Huang, Shouhong Guang, Chengming Zhu

{"title":"Peri-centrosomal localization of small interfering RNAs in C. elegans.","authors":"Qile Jin, Xuezhu Feng, Minjie Hong, Ke Wang, Xiangyang Chen, Jiewei Cheng, Yan Kuang, Xiaoyue Si, Mingjing Xu, Xinya Huang, Shouhong Guang, Chengming Zhu","doi":"10.1007/s11427-024-2818-7","DOIUrl":"10.1007/s11427-024-2818-7","url":null,"abstract":"The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs (cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering (si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material (PCM) components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase (RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material (PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"895-911"},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective G6PDH inactivation for Helicobacter pylori eradication with transformed polysulfide. G6PDH选择性失活转化多硫化物根除幽门螺杆菌。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2025-01-14 DOI: 10.1007/s11427-024-2775-3

Xiaonan Wang, Ning Zhou, Xuejiao J Gao, Zijing Zhu, Minmin Sun, Qian Wang, Haolin Cao, Xuetong Wu, Caiyu Zhou, Qingkang Zheng, Ye Yuan, Yuan Liu, Lei Chen, Jing Jiang, Pengcheng Bu, Lizeng Gao

{"title":"Selective G6PDH inactivation for Helicobacter pylori eradication with transformed polysulfide.","authors":"Xiaonan Wang, Ning Zhou, Xuejiao J Gao, Zijing Zhu, Minmin Sun, Qian Wang, Haolin Cao, Xuetong Wu, Caiyu Zhou, Qingkang Zheng, Ye Yuan, Yuan Liu, Lei Chen, Jing Jiang, Pengcheng Bu, Lizeng Gao","doi":"10.1007/s11427-024-2775-3","DOIUrl":"10.1007/s11427-024-2775-3","url":null,"abstract":"Alternative treatment for the highly prevalent Helicobacter pylori infection is imperative due to rising antibiotic resistance. We unexpectedly discovered that the anti-H. pylori component in garlic is hydrogen polysulfide (H2Sn, n⩾2), not organic polysulfides. Studies on the mechanism of action (MoA) show that H2Sn specifically inactivates H. pylori glucose-6-phosphate dehydrogenase (G6PDH) by interfering with electron transfer from glucose-6-phosphate (G6P) to nicotinamide adenine dinucleotide phosphate (NADP+). However, low H2Sn yield makes garlic derivatives hard to be a reliable donor of H2Sn to treat H. pylori infection. To address this challenge, we established a polysulfide transformation process from garlic organosulfur compounds into Fe3S4 that generates H2Sn with a 25-58 times increase in yield. Through chitosan encapsulation, we designed a gastric-adaptive H2Sn microreactor (GAPSR) that eradicates H. pylori with 250 times higher efficiency under gastric conditions. A single GAPSR achieves more rapid H. pylori eradication than combined antibiotics therapy without disturbing the gut microbiota. These findings indicate a distinct MoA transformation mediated by polysulfide as an alternative candidate to treat H. pylori infection.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"1158-1173"},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function.

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 DOI: 10.1007/s11427-024-2892-1

Pan Gong, Mengxin Gao, Yalin Chen, Mingzhen Zhang, Yucong Huang, Xiaohua Hu, Siwen Zhao, Hui Zhang, Mengjiao Pan, Buwei Cao, Qingtang Shen, Yong Liu, Rosa Lozano-Durán, Aiming Wang, Xueping Zhou, Fangfang Li

{"title":"Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function.","authors":"Pan Gong, Mengxin Gao, Yalin Chen, Mingzhen Zhang, Yucong Huang, Xiaohua Hu, Siwen Zhao, Hui Zhang, Mengjiao Pan, Buwei Cao, Qingtang Shen, Yong Liu, Rosa Lozano-Durán, Aiming Wang, Xueping Zhou, Fangfang Li","doi":"10.1007/s11427-024-2892-1","DOIUrl":"https://doi.org/10.1007/s11427-024-2892-1","url":null,"abstract":"The vast majority of known viruses belong to the positive-sense single-stranded RNA (+ssRNA) class. Tobamoviruses are among the most destructive plant viruses and threaten global food security. It is generally accepted that +ssRNA viruses including tobamoviruses encode proteins solely on their positive strand (+RNA). Here, we identified additional open-reading frames (ORFs) in the negative strand of tobamoviruses, named reverse ORFs (rORFs). Using cucumber green mottle mosaic virus (CGMMV) as a model, we detected the corresponding peptides of rORFs by mass spectrometry analysis and confirmed the translation of rORFs by ribosome profiling. Furthermore, we demonstrated that these rORFs may be translated from an internal ribosome entry site. Mutation of rORF1 and rORF2 significantly reduced the virulence of CGMMV, whereas ectopic expression of rORF1 and rORF2 could rescue the pathogenicity of the mutants. While the rORF2 protein localizes at the cell membrane and in the nucleolus, rORF1 colocalizes with peroxisomes, where it interacts with the viral 126-kD replication protein. Additionally, we screened peroxisomal rORF1-interacting proteins using artificial intelligence tools and found that PEX3 mediated rORF1 targeting to peroxisomes. This study reveals that the tobamoviral proteome is larger than previously thought, and sheds light on peroxisomes as novel virulence targets important for virus infectivity.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profile of Chao Liu. 刘超简介。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 DOI: 10.1007/s11427-024-2723-8

引用次数: 0

Not just a combination: a fully-integrated strategy of cellular therapy for acute myeloid leukemia expressing CD7, a heretofore elusive target. 不仅仅是一种组合：一种完全整合的细胞治疗策略，用于表达CD7的急性髓性白血病，这是一个迄今为止难以捉摸的目标。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2024-12-20 DOI: 10.1007/s11427-024-2711-y

Norbert-Claude Gorin

引用次数: 0

Histone methylation reprogramming underpins gene expression dynamics in Phytophthora during host infection. 组蛋白甲基化重编程支持宿主感染期间疫霉基因表达动态。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2024-12-11 DOI: 10.1007/s11427-024-2780-4

Haidong Shu, Guoyu Yin, Meihui Wu, Yujie Fang, Yufeng Fang, Yuanchao Wang, Han Chen, Suomeng Dong

引用次数: 0

Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces. 利用链霉菌的I-E型CRISPR/Cas系统对链霉菌进行高效的基因组编辑。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2025-04-01 Epub Date: 2025-01-14 DOI: 10.1007/s11427-024-2677-4

Yuhui Xie, Xiaoyan Liu, Tingting Wu, Yunzi Luo

{"title":"Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces.","authors":"Yuhui Xie, Xiaoyan Liu, Tingting Wu, Yunzi Luo","doi":"10.1007/s11427-024-2677-4","DOIUrl":"10.1007/s11427-024-2677-4","url":null,"abstract":"Since their discovery, CRISPR/Cas systems have significantly expanded the genetic toolbox, aiding in the exploration and enhanced production of natural products across various microbes. Among these, class 2 CRISPR/Cas systems are simpler and more broadly used, but they frequently fail to function effectively in many Streptomyces strains. In this study, we present an engineered class 1 type I CRISPR/Cas system derived from Streptomyces avermitilis, which enables efficient gene editing in phylogenetically distant Streptomyces strains. Through a plasmid interference assay, we identified the effective protospacer adjacent motif as 5'-AAN-3'. Utilizing this system, we achieved targeted chromosomal deletions ranging from 8 bp to 100 kb, with efficiencies exceeding 92%. We further utilized this system to insert DNA fragments into different Streptomyces genomes, facilitating the heterologous expression of exogenous genes and the activation of endogenous natural product biosynthetic gene clusters. Overall, we established a type I CRISPR/Cas-based gene-editing methodology that significantly advances the exploration of Streptomyces, known for their rich natural product resources. This is the first report of a gene editing tool developed based on the endogenous class 1 type I CRISPR/Cas system in Streptomyces spp. Our work enriches the Streptomyces gene manipulation toolbox and advances the discovery of valuable natural products within these organisms.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"1174-1182"},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0