Science China Life Sciences最新文献

{"title":"Therapeutic hypothermia for acute ischemic stroke: from preclinical studies to clinical trials.","authors":"Xi Chen, Jin Xu, Jiachen He, Jiaqi Guo, Shuaili Xu, Xuefan Yao, Yuanyuan Liu, Xiaohan Xu, Huimin Wei, Ming Li, Chuanjie Wu, Longfei Wu, Marc Fisher, Xunming Ji, Di Wu","doi":"10.1007/s11427-024-2738-2","DOIUrl":"10.1007/s11427-024-2738-2","url":null,"abstract":"<p><p>Therapeutic hypothermia (TH) is acknowledged as a promising neuroprotective strategy in clinical settings. However, its application in managing acute ischemic stroke (AIS) remains unclear due to variable clinical outcomes in bench and bedsides. A comprehensive review of original studies concerning hypothermia in ischemic stroke was conducted, sourcing data from PubMed, Web of Science, Embase, and Ovid Medicine databases covering the period from January 1, 1990 to October 31, 2023. Our search strategy yielded 1,218 articles from PubMed, 1,094 from Web of Science, 3,083 from Embase, and 2,841 from Ovid Medicine. After removing duplicates, review articles, meta-analyses, and in vitro studies focusing on hypoxic-ischemic encephalopathy or global cerebral ischemia, a total of 304 articles out of 5,669 papers were ultimately selected for in-depth analysis. Overall, we have found that there are significant differences in depth, duration, and delay between bench and bedside studies. We want to introduce the concepts of \"actual brain temperature\", \"hypothermia initiation time\", and \"effective hypothermic duration\" as crucial for the optimization of hypothermic therapy in AIS. We recommend critical parameters for the clinical translation of hypothermia, including a target temperature range of 34-35°C, a duration of 2-4 h, immediate initiation post-insult, and natural rewarming processes. We also advocate for selective brain cooling when reperfusion therapy is achieved. We find great differences in administrating TH for AIS between bench and bedsides. More efforts are still needed to enhance the likelihood of successful clinical translation and deepen the understanding of hypothermia's role in AIS treatment.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2867-2879"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial infection in weaned piglets promotes diarrhea by inducing the NLRP3 inflammasome-pyroptosis pathway.","authors":"Jie Fu, Zipeng Jiang, Chaoyue Wen, Weifa Su, Mingzhi Yang, Huan He, Yalin Liu, Tao Gong, Shouchuan Jiang, Fengqin Wang, Zeqing Lu, Mingliang Jin, Yizhen Wang","doi":"10.1007/s11427-024-2728-2","DOIUrl":"10.1007/s11427-024-2728-2","url":null,"abstract":"<p><p>Bacterial infections in weaned piglets are a major cause of diarrhea and even death. However, the mechanism by which bacterial infections in weaned piglets lead to diarrhea remains unclear. The NLRP3 inflammasome and pyroptosis are considered key factors in mucosal immune damage. Therefore, we aimed to investigate whether bacterial infections in weaned piglets promote diarrhea through the NLRP3 inflammasome-pyroptosis pathway. Our research findings indicate that compared with weaned healthy piglets, those with weaning diarrhea exhibit activation of the NLRP3 inflammasome and pyroptosis. Weaned piglets with diarrhea had reduced microbiota diversity in the gut, significant differences in β-diversity, a notable increase in the ratio of Firmicutes to Bacteroidetes, and a significant increase in the abundance of pathogens, such as Escherichia coli and Clostridium perfringens. Further infection experiments with enterotoxigenic Escherichia coli (ETEC) K88 and C. perfringen in weaned piglets revealed that these pathogens disrupt intestinal integrity, promote diarrhea incidence, increase serum IL-1β and IL-18 levels, and activate the NLRP3 inflammasome-pyroptosis pathway. Mechanistic studies using NLRP3 knockout mice showed significant alleviation of elevated serum IL-1β and IL-18 levels, intestinal inflammatory cell infiltration, downregulation of intestinal barrier protein gene expression, and reduction in diarrhea incidence caused by ETEC K88 and C. perfringen infection. Finally, we investigated the role of sow's breast-milk immunoglobulins in activating the NLRP3 inflammasome-pyroptosis pathway and the incidence of diarrhea in weaned piglets. The results demonstrate that sow's breast milk immunoglobulins are beneficial for protecting weaned piglets from pathogenic bacterial infection-induced NLRP3 inflammasome activation and diarrhea occurrence. In conclusion, our study elucidates the inflammatory mechanisms underlying diarrhea occurrence in piglets due to reduced levels of immunoglobulins from sow's breast milk after weaning, thereby promoting NLRP3 inflammasome-pyroptosis pathway activation mediated by pathogenic bacterial infection.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"3021-3036"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver sinusoidal endothelial cells secret C-X-C motif chemokine ligand 10 to promote the recruitment of invariant NKT cells in acetaminophen-induced liver injury.","authors":"Fei Wang, Shuangshuang Zhang, Yan Yao, Penghui Yu, Ke Xue, Xuan Xu, Gaoxiang Li, Hong Zhou","doi":"10.1007/s11427-025-2942-8","DOIUrl":"10.1007/s11427-025-2942-8","url":null,"abstract":"<p><p>Drug-induced liver injury (DILI) has become a significant public health concern. Liver sinusoidal endothelial cells (LSECs), serving as the primary defense barrier in the liver, play a crucial role in maintaining the sinusoidal microenvironment. However, the adaptive changes occurring in LSECs and the mechanisms that regulate the immune microenvironment during the initial stages of acetaminophen (APAP)-induced liver injury (AILI) remain unclear. Specifically, a significant knowledge gap remains regarding how LSECs interact with immune cells in AILI. In our study, we observed distinct morphological changes in the LSECs during the early stages of AILI. Using single-nuclear RNA sequencing, we identified a disease-specific subpopulation of LSECs, characterized by significant enrichment of biological processes associated with vascular remodeling and cell migration following APAP treatment. Simultaneously, APAP enhanced intercellular communication between LSECs and T/NK cells, with an emphasis on invariant natural killer T (iNKT) cells. Specifically, this LSEC population exhibited significant upregulation of CXCL10, a chemokine that plays a pivotal role in the recruitment of hepatic iNKT and CD4⁺ T cells. Pharmacological inhibition of CXCR3, a receptor for CXCL10, using AMG487 effectively blocked the APAP-induced recruitment of these immune cells. In summary, our study elucidates the distinctive alterations in LSECs associated with early AILI and identifies the CXCL10-CXCR3 axis as a critical pathway mediating the recruitment of hepatic iNKT and CD4⁺ T cells. These findings provide valuable insights into the development of novel therapeutic strategies aimed at targeting the CXCL10-CXCR3 signaling axis for the treatment of AILI.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2982-2994"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective expansion of TCF7-expressing tumor-reactive T cell subpopulations during ovarian tumor-infiltrating T cell production ex vivo.","authors":"Dingfeng Liu, Ting Zhang, Qinli Sun, Dongli Cai, Yanan Lou, Genyu Wang, Bowen Xie, Yicheng Zhu, Chong Wang, Zhouping Lu, Chenfei Liu, Yuan Li, Xi Zhang, Tianhui He, Jing Hao, Xinyi Guo, Jiaming Li, Xiaowei Xi, Ling Ni, Hongyan Guo, Jing Ge, Liping Jin, Chen Dong","doi":"10.1007/s11427-025-2958-3","DOIUrl":"10.1007/s11427-025-2958-3","url":null,"abstract":"<p><p>Tumor-infiltrating lymphocyte (TIL) therapy was recently approved for melanoma patients; however, the dynamic changes in T cell subpopulations during TIL production remain poorly understood. Here, we analyzed epithelial ovarian cancer samples at various stages of ex vivo TIL culture using paired single-cell RNA and TCR sequencing. We also assessed the expansion potential and tumor reactivity of the identified TIL subpopulations. Single-cell transcriptomic analysis revealed that CD8⁺ TILs exhibited reduced cellular diversity following ex vivo expansion, selectively expanding stem-like TCF7⁺ precursors of exhausted T cells (Tpex) and effector-like tissue-resident memory (Trm) cells. TCR clonotype analysis showed that Tpex cells accumulated through self-renewal, while Trm cells primarily originated from TCF7⁺GZMK⁺ early effector memory cells in tumors. Additionally, TCR tracing identified preferential activation and reprogramming of CD4⁺ T follicular helper (Tfh)-like cells, especially TCF7⁺ ones. All three TCF7⁺ subpopulations showed robust expansion potential and tumor reactivity in vitro. Notably, CCR7⁺CD200⁺ T cells, enriched for TCF-1⁺CD8⁺ Tpex and CD4⁺ Tfh-like cells in the tumor microenvironment, exhibited self-renewal during in vitro expansion and demonstrated tumor reactivity both in vivo and in vitro. These findings highlight the selective expansion of tumor-reactive TCF7⁺ T cells during TIL culture and suggest that CCR7 and CD200 serve as important surface markers for generating stem-like, tumor-reactive cells, potentially improving TIL therapy in cancers.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2891-2907"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocrine cancer organoids in basic and translational medical research.","authors":"Dong Chen, Zhulan Chen, Huihui Yang, Lei Zhang, Chenchen Hu, Zhuo Yang, Peng Li, Xi Su, Xiaoling Liu, Wei Wei, Yongsheng Zhao","doi":"10.1007/s11427-024-2888-8","DOIUrl":"10.1007/s11427-024-2888-8","url":null,"abstract":"<p><p>Endocrine cancers are a heterogeneous group of malignancies that originate from cells capable of secreting hormones. Examples include but are not limited to thyroid cancer, adrenocortical carcinoma, prostate cancer, and pancreatic cancer. Our limited understanding of endocrine cancers is partially due to constraints related to model systems, which cannot accurately replicate the pathogenesis of these tumors. Patient-derived organoids (PDOs) are clusters of multiple cell types that grow in a three-dimensional environment. They have become innovative models that faithfully reproduce genotype and phenotype of the tissues from which they originated, facilitating the prediction of patient treatment responses and guiding the development of precision medicine. This article provides a comprehensive review of the establishment of endocrine cancer PDOs and their applications in cancer research, drug screening, and personalized therapy. These excellent preclinical models have the potential to advance our understanding of endocrine cancers in basic research and clinical practice. In addition, we discuss the challenges related to current organoid technologies and provide future perspectives on the applications of organoids in precision medicine to improve the management of endocrine cancers.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2842-2866"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic WNT signaling controls differentiation of hematopoietic progenitor cells from human pluripotent stem cells.","authors":"Mo Li, Keiichiro Suzuki, Mengge Wang, Christopher Benner, Manching Ku, Li Ma, Ladan Kobari, Na Young Kim, Nuria Montserrat, Chan-Jung Chang, Guanghui Liu, Jing Qu, Jinna Xu, Yingzi Zhang, Emi Aizawa, Jun Wu, Luc Douay, Concepcion Rodriguez Esteban, Juan Carlos Izpisua Belmonte","doi":"10.1007/s11427-024-2816-0","DOIUrl":"10.1007/s11427-024-2816-0","url":null,"abstract":"<p><p>Human pluripotent stem cells (hPSCs) can in theory give rise to any hematopoietic lineages, thereby offering opportunities for disease modeling, drug screening and cell therapies. However, gaps in our knowledge of the signaling requirements for the specification of human hematopoietic stem/progenitor cells (HSPCs), which lie at the apex of all hematopoietic lineages, greatly limit the potential of hPSC in hematological research and application. Transcriptomic analysis reveals aberrant regulation of WNT signaling during maturation of hPSC-derived hematopoietic progenitor cells (hPSC-HPCs), which results in higher mitochondria activity, misregulation of HOX genes, loss of self-renewal and precocious differentiation. These defects are partly due to the activation of the WNT target gene CDX2. Late-stage WNT inhibition improves the yield, self-renewal, multilineage differentiation, and transcriptional and metabolic profiles of hPSC-HPCs. Genome-wide mapping of transcription factor (TF) accessible chromatin reveals a significant overrepresentation of myeloid TF binding motifs in hPSC-HPCs, which could underlie their myeloid-biased lineage potential. Together our findings uncover a previously unappreciated dynamic requirement of the WNT signaling pathway during the specification of human HSPCs. Modulating the WNT pathway with small molecules normalizes the molecular differences between hPSC-HPCs and endogenous hematopoietic stem cells (HSCs), thereby representing a promising approach to improve the differentiation and function of hPSC-HPCs.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2829-2841"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEX5 deficiency enhances radiosensitivity via MGST1-GSH detoxifying function and promotes ferroptosis in liver cancer.","authors":"Zhuhui Yuan, Tong Liu, Ping Jiang, Yang Zhao, Ruiping Guo, Xiaoyong Ye, Qiang He, Hao Wang, Lixiang Xue, Junjie Wang","doi":"10.1007/s11427-024-2938-3","DOIUrl":"10.1007/s11427-024-2938-3","url":null,"abstract":"<p><p>Recent research underscores a growing connection between liver cancer irradiation resistance and ferroptosis, with glutathione (GSH) serving as a pivotal factor in this resistance by mitigating oxidative stress. Despite the crucial role of peroxisomes in modulating this redox balance, the influence of peroxisome biogenesis gene 5 (PEX5), a highly expressed gene in liver cancer cells that regulates peroxisome formation, on GSH-mediated resistance to radiation-induced ferroptosis remains poorly understood. We employed targeted metabolomics, RNA sequencing, and Raman spectroscopy, in radioresistant liver tumor cell models, human liver cancer organoids, and mouse model to investigate the phenomenon and potential mechanisms. Our findings revealed that combining ferroptosis inducers and radiotherapy (IR) effectively overcame radioresistance in both radioresistant liver tumor cells (IRR) and human liver cancer organoids. Notably, we observed a correlation between increased levels of amino acids involved in GSH synthesis and IR-induced ferroptosis resistance in IRR cells, indicating that GSH plays a significant protective role against ferroptosis in these cells. Furthermore, exogenous GSH supplementation was found to potentiate resistance to IR-induced ferroptosis, further confirming the protective role of GSH. However, suppression of PEX5, a previously identified radiosensitivity regulator, reversed GSH-mediated IR-ferroptosis resistance. Specifically, PEX5 suppression enhanced ferroptosis activation and radiation damage by impairing GSH function. Mechanistically, PEX5 binds to the transmembrane domain of MGST1, a crucial regulator for GSH detoxification. By inhibiting PEX5, we disrupted MGST1 functionality, resulting in the attenuation of radioresistance and the enhancement of ferroptosis, as MGST1's role in GSH detoxification was impaired, thereby increasing vulnerability to radiation-induced cellular ferroptosis. PEX5 deficiency induces ferroptosis by impairing the MGST1-GSH detoxification pathway, thereby enhancing radiation-induced damage via the regulation of redox balance. This novel function of PEX5 offers new insight in ferroptosis mediated by peroxisome and provides the potential novel therapeutic strategy to enhance the anti-tumor effects of radiation.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2908-2922"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlock secrets to eternal youth: gene-hacked human stem cells combat aging in primates.","authors":"Ying Wang, Yufang Shi","doi":"10.1007/s11427-025-3001-1","DOIUrl":"10.1007/s11427-025-3001-1","url":null,"abstract":"","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"3125-3127"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization patterns of intestinal pioneering microbiota of different broiler breeds and their effects on composition of intestinal mucosal barrier during early life.","authors":"Yan Hu, Zhuang Liu, Hong Yao, Shu Wu, Qianyun Zhang, Shifeng Pan, Shourong Shi","doi":"10.1007/s11427-024-2873-3","DOIUrl":"10.1007/s11427-024-2873-3","url":null,"abstract":"<p><p>Intestinal pioneering microbiota can affect host growth, development, and health via microbial programming. However, the presence of microbial colonization in the intestine of embryonic chickens, development and colonization patterns of intestinal pioneering microbiota of different broiler breeds and their effects on the composition of intestinal mucosal barrier during early life remain unknown. Arbor Acres (AA) chickens exhibiting high growth efficiency traits and Chinese local Tibetan chickens exhibiting high environmental adaptability traits were used as experimental animals to verify the absence of bacterial colonization and a sterile state in embryonic chickens intestine under normal maternal health. During neonatal early stage, jejunal mucosal structure and barrier function of AA chickens with higher growth efficiency were more conducive to digestion and absorption, corresponding to persistently higher microbial maturity, whereas those of Tibetan chickens with lower growth efficiency were more conducive to stress resistance, corresponding to lower microbial maturity. Colonization patterns of intestinal pioneering microbiota were significantly different between the two breeds. The dominant microbiota of AA chickens, such as Erysipelatoclostridium, Hydrogenoanalobacterium and Shuttleworthia, were related to growth and metabolic functions, whereas those of Tibetan chickens, such as Limosilactobacillus, Ligilactobacillus and Prevotella, were related to immune and anti-stress functions. Transplanting intestinal pioneering microbiota of the donor could transfer the abundance of dominant microbiota to the recipient in a symbiotic state. Growth efficiency and adaptability of transplanted AA chickens improved, accompanied by optimized jejunal mucosal structure and function. However, the growth efficiency of transplanted Tibetan chickens was not affected by the modified microbiota diversity. It was suggested that cross-FMT technology achieved inter-breed complementary advantages of high growth efficiency and high adaptability traits of broilers during neonatal early life; the higher maturity of intestinal pioneering microbiota of the recipient, the more growth efficiency of the recipient would be susceptibly affected by transplanting intestinal pioneering microbiota of the donor.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"3051-3073"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alfalfa pan-genome unveiled-a breakthrough in alfalfa genomics-assisted breeding.","authors":"Gai Huang, Lulu Li, Xiaofeng Cao, Hao Lin","doi":"10.1007/s11427-025-2995-7","DOIUrl":"10.1007/s11427-025-2995-7","url":null,"abstract":"","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"3131-3132"},"PeriodicalIF":9.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}